Massilia eurypsychrophila

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Oxalobacteraceae; Massilia

Average proteome isoelectric point is 6.85

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5261 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2G8TJ22|A0A2G8TJ22_9BURK Methionine aminotransferase OS=Massilia eurypsychrophila OX=1485217 GN=CR105_07840 PE=4 SV=1
MM1 pKa = 7.1LTALNALQSTAQPFTVDD18 pKa = 4.28VIDD21 pKa = 4.06VDD23 pKa = 3.94ADD25 pKa = 3.65PALVARR31 pKa = 11.84FDD33 pKa = 3.69EE34 pKa = 4.84LVPVLYY40 pKa = 10.75GDD42 pKa = 4.36LAAPEE47 pKa = 4.36LCHH50 pKa = 6.6YY51 pKa = 10.12FLDD54 pKa = 4.26AAAVRR59 pKa = 11.84AYY61 pKa = 10.22LASDD65 pKa = 3.48HH66 pKa = 6.72LSPGG70 pKa = 3.32

Molecular weight: 7.46 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2G8TFZ6|A0A2G8TFZ6_9BURK DUF2807 domain-containing protein OS=Massilia eurypsychrophila OX=1485217 GN=CR105_10840 PE=4 SV=1
MM1 pKa = 7.51NLRR4 pKa = 11.84THH6 pKa = 6.32SRR8 pKa = 11.84PFAFAMLAMLFSLASHH24 pKa = 6.77RR25 pKa = 11.84AARR28 pKa = 11.84LQLRR32 pKa = 11.84RR33 pKa = 11.84PPAGLSGAPARR44 pKa = 11.84VRR46 pKa = 11.84RR47 pKa = 11.84LTFGNPIRR55 pKa = 11.84RR56 pKa = 11.84LGLLAGAHH64 pKa = 5.88

Molecular weight: 7.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5261

0

5261

1730067

27

2951

328.8

35.64

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.035 ± 0.059

0.895 ± 0.012

5.443 ± 0.027

5.026 ± 0.032

3.647 ± 0.019

8.161 ± 0.034

2.142 ± 0.017

4.844 ± 0.024

3.644 ± 0.034

10.316 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.696 ± 0.017

2.978 ± 0.022

4.855 ± 0.025

3.797 ± 0.022

6.508 ± 0.033

5.615 ± 0.026

5.191 ± 0.027

7.501 ± 0.026

1.29 ± 0.013

2.416 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski