Fomitopsis rosea
Average proteome isoelectric point is 6.37
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 11196 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4Y9YJ24|A0A4Y9YJ24_9APHY Peptidase S53 domain-containing protein OS=Fomitopsis rosea OX=34475 GN=EVJ58_g4155 PE=4 SV=1
MM1 pKa = 6.85 NTFFFGLLLASLCGLTNALYY21 pKa = 9.64 IPVPVRR27 pKa = 11.84 HH28 pKa = 6.7 DD29 pKa = 3.48 AQVSSRR35 pKa = 11.84 GTGVHH40 pKa = 5.92 NPAIRR45 pKa = 11.84 LVGAFRR51 pKa = 11.84 QQDD54 pKa = 3.51 ANNDD58 pKa = 3.75 SVVNFQDD65 pKa = 3.14 TVYY68 pKa = 9.36 ATNMTIGGQDD78 pKa = 4.28 LLIQLDD84 pKa = 4.04 TGSSDD89 pKa = 2.75 LWVNLEE95 pKa = 3.9 AGEE98 pKa = 4.55 IEE100 pKa = 4.52 FTNTTDD106 pKa = 3.86 LPAEE110 pKa = 3.8 EE111 pKa = 5.32 AYY113 pKa = 10.56 GIGHH117 pKa = 6.23 ITGTIQFANAEE128 pKa = 4.27 LGDD131 pKa = 4.23 NIVPNQAFMNVSNATSFSTIFNDD154 pKa = 4.17 GVRR157 pKa = 11.84 GILGLAFDD165 pKa = 5.15 AGSTVQGTIAQVYY178 pKa = 10.22 GEE180 pKa = 4.97 DD181 pKa = 3.65 STLGRR186 pKa = 11.84 SFLSNLFNQNTTKK199 pKa = 10.68 PLFTVQLGRR208 pKa = 11.84 TDD210 pKa = 3.84 DD211 pKa = 3.88 PQYY214 pKa = 9.14 TAEE217 pKa = 4.11 GAFTIGEE224 pKa = 4.28 YY225 pKa = 10.82 LPDD228 pKa = 3.83 YY229 pKa = 11.32 DD230 pKa = 5.2 EE231 pKa = 4.73 VQDD234 pKa = 3.72 MPKK237 pKa = 10.41 LHH239 pKa = 7.1 RR240 pKa = 11.84 FPNNGTGAAPRR251 pKa = 11.84 WSTVMSGMTINGEE264 pKa = 3.84 PFQFNASGVPDD275 pKa = 3.94 TPEE278 pKa = 4.28 GSVVAVLDD286 pKa = 3.59 TGFTFPPIPGPAVEE300 pKa = 5.51 AIYY303 pKa = 10.8 SGIQGAYY310 pKa = 9.45 YY311 pKa = 8.51 DD312 pKa = 4.57 TNSSLWIVPCNKK324 pKa = 9.6 AANVEE329 pKa = 4.35 FQFGDD334 pKa = 3.34 MSVPIHH340 pKa = 6.54 PLDD343 pKa = 3.42 LTTIVTINNTEE354 pKa = 3.85 VCVNTFRR361 pKa = 11.84 PSTFPVNNQFDD372 pKa = 4.7 LVLGDD377 pKa = 4.55 AFLKK381 pKa = 10.3 NVYY384 pKa = 10.13 VSFNYY389 pKa = 10.86 GDD391 pKa = 3.7 MSADD395 pKa = 3.16 AVPFMQIMPTTDD407 pKa = 2.39 MDD409 pKa = 4.18 AATKK413 pKa = 10.23 EE414 pKa = 4.14 FASVRR419 pKa = 11.84 GAMYY423 pKa = 10.97 AEE425 pKa = 4.6 AAASAAASSASASAASAYY443 pKa = 10.08 AALEE447 pKa = 4.36 SGSASVPCNAGAPTTTPLFTSAWSSSPSAVMTDD480 pKa = 3.4 SAPANTDD487 pKa = 3.2 SPSLYY492 pKa = 10.46 DD493 pKa = 3.83 PSSGDD498 pKa = 3.34 GSSDD502 pKa = 3.18 ATPSSSADD510 pKa = 3.09 ASSATDD516 pKa = 3.26 ASSPDD521 pKa = 3.65 SASPAATTDD530 pKa = 3.11 AATDD534 pKa = 3.58 ASSTDD539 pKa = 3.24 SSSPTTTSDD548 pKa = 3.72 ASSTDD553 pKa = 3.19 ASATATDD560 pKa = 3.96 ASADD564 pKa = 3.58 ATATPTDD571 pKa = 3.75 ASSPTATSDD580 pKa = 3.67 ASDD583 pKa = 3.61 ASSTDD588 pKa = 3.2 SSASTTDD595 pKa = 2.82 ASTDD599 pKa = 3.24 ATATTTDD606 pKa = 3.07 ASADD610 pKa = 3.52 ATATPTDD617 pKa = 3.87 TPADD621 pKa = 3.53 AATTTDD627 pKa = 3.42 ASATSTDD634 pKa = 3.14 SSADD638 pKa = 3.29 ATATPDD644 pKa = 3.7 DD645 pKa = 4.54 SSDD648 pKa = 3.57 NSSTGSLPIDD658 pKa = 4.2 PSASADD664 pKa = 3.76 DD665 pKa = 4.61 ASSSSDD671 pKa = 3.57 DD672 pKa = 3.7 GSGSLSEE679 pKa = 4.21 NLSLLGSPEE688 pKa = 4.19 SKK690 pKa = 10.21 QSSFGGNLYY699 pKa = 10.64 GSNDD703 pKa = 3.34 YY704 pKa = 10.68 EE705 pKa = 4.07 YY706 pKa = 10.9 SRR708 pKa = 11.84 RR709 pKa = 11.84 FRR711 pKa = 11.84 RR712 pKa = 11.84 SYY714 pKa = 10.88 RR715 pKa = 11.84 EE716 pKa = 3.58 PTKK719 pKa = 10.27 TVHH722 pKa = 6.21 EE723 pKa = 4.45 CLAPLIEE730 pKa = 5.66 DD731 pKa = 3.3 YY732 pKa = 11.42 GAFLFALIAGTFIISFALCVVTVFLAIRR760 pKa = 11.84 NHH762 pKa = 4.6 TRR764 pKa = 11.84 RR765 pKa = 11.84 DD766 pKa = 3.19 ARR768 pKa = 11.84 KK769 pKa = 7.95 RR770 pKa = 11.84 AAYY773 pKa = 9.8 LVLEE777 pKa = 4.66 ANAEE781 pKa = 4.07 AEE783 pKa = 4.38 ADD785 pKa = 3.96 PEE787 pKa = 4.13 AAAYY791 pKa = 7.1 TATYY795 pKa = 9.86 HH796 pKa = 5.37 YY797 pKa = 9.57 TDD799 pKa = 5.13 DD800 pKa = 3.95 YY801 pKa = 11.95
Molecular weight: 83.02 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.676
IPC2_protein 3.732
IPC_protein 3.783
Toseland 3.554
ProMoST 3.961
Dawson 3.795
Bjellqvist 3.935
Wikipedia 3.732
Rodwell 3.605
Grimsley 3.452
Solomon 3.783
Lehninger 3.745
Nozaki 3.897
DTASelect 4.19
Thurlkill 3.605
EMBOSS 3.745
Sillero 3.91
Patrickios 1.494
IPC_peptide 3.783
IPC2_peptide 3.884
IPC2.peptide.svr19 3.801
Protein with the highest isoelectric point:
>tr|A0A4Y9XX56|A0A4Y9XX56_9APHY Uncharacterized protein OS=Fomitopsis rosea OX=34475 GN=EVJ58_g8964 PE=4 SV=1
MM1 pKa = 7.3 TLLTKK6 pKa = 10.87 SMAPGADD13 pKa = 3.48 SQIGTSARR21 pKa = 11.84 RR22 pKa = 11.84 HH23 pKa = 4.41 RR24 pKa = 11.84 TAVGAPIVRR33 pKa = 11.84 NCAHH37 pKa = 6.73 RR38 pKa = 11.84 RR39 pKa = 11.84 HH40 pKa = 6.69 PKK42 pKa = 8.34 RR43 pKa = 11.84 THH45 pKa = 6.36 ASTTRR50 pKa = 11.84 RR51 pKa = 11.84 RR52 pKa = 11.84 RR53 pKa = 11.84 RR54 pKa = 11.84 PDD56 pKa = 2.73 ARR58 pKa = 11.84 RR59 pKa = 11.84 GWSSRR64 pKa = 11.84 CRR66 pKa = 11.84 ASASLRR72 pKa = 11.84 RR73 pKa = 11.84 VRR75 pKa = 11.84 WSSSRR80 pKa = 11.84 RR81 pKa = 11.84 RR82 pKa = 11.84 STGGRR87 pKa = 11.84 SRR89 pKa = 11.84 ASGTRR94 pKa = 11.84 SASPARR100 pKa = 11.84 RR101 pKa = 11.84 GKK103 pKa = 10.32 SSPFACSTLNPYY115 pKa = 8.2 ITILLTFLATAXKK128 pKa = 9.74 DD129 pKa = 3.48 AQALXVLEE137 pKa = 5.4 RR138 pKa = 11.84 FIPWADD144 pKa = 3.24 LAVFLTAIPRR154 pKa = 11.84 RR155 pKa = 11.84 VTYY158 pKa = 10.49 RR159 pKa = 11.84 EE160 pKa = 3.9 QQKK163 pKa = 9.77 EE164 pKa = 4.06 RR165 pKa = 11.84 SDD167 pKa = 3.74 SAVLLTSGCKK177 pKa = 9.46 PLPEE181 pKa = 4.38 DD182 pKa = 2.88 WCLRR186 pKa = 11.84 GLGWGGKK193 pKa = 8.16 RR194 pKa = 11.84 VYY196 pKa = 10.97 PMGFWGKK203 pKa = 8.76 EE204 pKa = 3.67 ANVEE208 pKa = 3.98 DD209 pKa = 4.64 RR210 pKa = 11.84 NVEE213 pKa = 4.03 VEE215 pKa = 4.3 VLDD218 pKa = 3.96 KK219 pKa = 11.68 SEE221 pKa = 5.97 GGDD224 pKa = 3.3 QLDD227 pKa = 5.14 GIIEE231 pKa = 4.11 DD232 pKa = 3.46 EE233 pKa = 4.38 RR234 pKa = 11.84 EE235 pKa = 3.86 EE236 pKa = 4.17 NEE238 pKa = 4.24 AANEE242 pKa = 4.42 LGKK245 pKa = 10.69 CWIRR249 pKa = 11.84 VACAGLKK256 pKa = 8.95 IAKK259 pKa = 9.39 HH260 pKa = 5.42 VGGFAYY266 pKa = 10.5 YY267 pKa = 9.05 PAANEE272 pKa = 3.91 EE273 pKa = 4.28 HH274 pKa = 7.16 RR275 pKa = 11.84 GQQ277 pKa = 3.8
Molecular weight: 30.64 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.354
IPC2_protein 9.516
IPC_protein 10.394
Toseland 10.687
ProMoST 10.438
Dawson 10.774
Bjellqvist 10.511
Wikipedia 10.994
Rodwell 10.877
Grimsley 10.818
Solomon 10.921
Lehninger 10.891
Nozaki 10.701
DTASelect 10.496
Thurlkill 10.687
EMBOSS 11.096
Sillero 10.716
Patrickios 10.584
IPC_peptide 10.935
IPC2_peptide 9.75
IPC2.peptide.svr19 8.651
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
11196
0
11196
5146711
12
5558
459.7
50.73
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.52 ± 0.019
1.187 ± 0.007
5.927 ± 0.015
6.181 ± 0.022
3.454 ± 0.013
6.525 ± 0.02
2.547 ± 0.01
4.406 ± 0.014
4.311 ± 0.022
9.077 ± 0.023
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.15 ± 0.009
3.098 ± 0.013
6.55 ± 0.027
3.729 ± 0.014
6.594 ± 0.02
8.004 ± 0.029
5.937 ± 0.016
6.606 ± 0.015
1.447 ± 0.007
2.718 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here