Planctomycetes bacterium Poly30

Taxonomy: cellular organisms; Bacteria; PVC group; Planctomycetes; unclassified Planctomycetes

Average proteome isoelectric point is 5.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5628 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A518EMG8|A0A518EMG8_9BACT Uncharacterized protein OS=Planctomycetes bacterium Poly30 OX=2528018 GN=Poly30_07810 PE=4 SV=1
MM1 pKa = 7.11QKK3 pKa = 10.53SLLFLAGSALLAIPASAQATFTLMGSGTAADD34 pKa = 4.0MNSDD38 pKa = 2.97GTAVVGRR45 pKa = 11.84DD46 pKa = 3.57GQGAFLWTATAGYY59 pKa = 8.2MPLGGSDD66 pKa = 3.34AVSVSEE72 pKa = 5.24DD73 pKa = 3.29GTVVLGNFTDD83 pKa = 3.76VNGMQSAGRR92 pKa = 11.84WTAATGWVSIGDD104 pKa = 4.1LGSSSGTSLSSAYY117 pKa = 10.27GMSGDD122 pKa = 3.8GNRR125 pKa = 11.84ATGLGWLNAGTAGAFEE141 pKa = 4.42WTPTSGTLQLPQLGPNSSRR160 pKa = 11.84GNCVSLDD167 pKa = 3.33GVYY170 pKa = 10.18IGGWDD175 pKa = 3.94EE176 pKa = 5.65DD177 pKa = 3.77PTGPRR182 pKa = 11.84RR183 pKa = 11.84ASIWGPSGTQFLPLVDD199 pKa = 4.03PVNNPEE205 pKa = 4.51GIGEE209 pKa = 4.27VFGLSSDD216 pKa = 3.18GAYY219 pKa = 10.44AVGGAGDD226 pKa = 3.93TAWLYY231 pKa = 10.46EE232 pKa = 4.26LSTGTLTDD240 pKa = 4.91FGLLPNCCGGFFDD253 pKa = 5.47RR254 pKa = 11.84GFSEE258 pKa = 4.84AVSDD262 pKa = 3.8DD263 pKa = 3.4GQRR266 pKa = 11.84VVGLFGSGPFSFVGTIWGPTSGYY289 pKa = 10.24EE290 pKa = 3.91RR291 pKa = 11.84LSDD294 pKa = 3.63VLVAAGANLQGFEE307 pKa = 4.31ITSGTDD313 pKa = 2.84ISPNGGIILGNAVPTGSFSGQWFIATLDD341 pKa = 3.8PTSGPGTVYY350 pKa = 8.97CTPAAANSTGAPATISTSGSDD371 pKa = 3.26SVANNDD377 pKa = 4.3LVLTASNLPNNSFAYY392 pKa = 9.74FINSMTQAMIVMPGGSQGTVCVGGSVGRR420 pKa = 11.84FLQQIQNSGSNGSISISVDD439 pKa = 3.45LVNLPQPNGLVSVMPGEE456 pKa = 4.31TWNFQAWYY464 pKa = 9.78RR465 pKa = 11.84DD466 pKa = 3.75ANPTSTSNLTDD477 pKa = 5.23AISVTFQQ484 pKa = 2.75

Molecular weight: 49.08 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A518ESU8|A0A518ESU8_9BACT EamA-like transporter family protein OS=Planctomycetes bacterium Poly30 OX=2528018 GN=Poly30_26880 PE=4 SV=1
MM1 pKa = 7.38KK2 pKa = 10.62LKK4 pKa = 10.4VRR6 pKa = 11.84NSKK9 pKa = 10.29AKK11 pKa = 9.59KK12 pKa = 8.82AKK14 pKa = 8.8KK15 pKa = 8.78TGFLTRR21 pKa = 11.84QKK23 pKa = 10.73SAGGRR28 pKa = 11.84KK29 pKa = 6.56TNKK32 pKa = 9.01RR33 pKa = 11.84QRR35 pKa = 11.84ARR37 pKa = 11.84HH38 pKa = 5.17GSFF41 pKa = 3.22

Molecular weight: 4.69 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5628

0

5628

2299414

30

2708

408.6

44.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.765 ± 0.044

0.824 ± 0.01

6.06 ± 0.022

6.666 ± 0.039

3.588 ± 0.018

9.305 ± 0.031

2.007 ± 0.013

4.005 ± 0.018

2.474 ± 0.026

10.282 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.95 ± 0.013

2.158 ± 0.022

5.604 ± 0.023

2.959 ± 0.016

7.533 ± 0.039

6.447 ± 0.026

5.406 ± 0.025

7.565 ± 0.025

1.472 ± 0.011

1.929 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski