Methanohalobium evestigatum (strain ATCC BAA-1072 / DSM 3721 / NBRC 107634 / OCM 161 / Z-7303)

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia; Methanosarcinales; Methanosarcinaceae; Methanohalobium; Methanohalobium evestigatum

Average proteome isoelectric point is 5.87

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2250 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D7E9Z3|D7E9Z3_METEZ HTH_45 domain-containing protein OS=Methanohalobium evestigatum (strain ATCC BAA-1072 / DSM 3721 / NBRC 107634 / OCM 161 / Z-7303) OX=644295 GN=Metev_1568 PE=4 SV=1
MM1 pKa = 7.29KK2 pKa = 10.55RR3 pKa = 11.84LMVLFAGLMAILMSISAASAITVDD27 pKa = 3.77GNKK30 pKa = 9.58TSGEE34 pKa = 3.9WDD36 pKa = 3.61EE37 pKa = 4.55NWAFGQTNNATAASEE52 pKa = 3.95YY53 pKa = 10.72DD54 pKa = 3.4INNLGDD60 pKa = 3.79RR61 pKa = 11.84LEE63 pKa = 4.3IMQGTLQADD72 pKa = 3.57TGDD75 pKa = 3.99YY76 pKa = 10.97NAVDD80 pKa = 4.27PKK82 pKa = 10.88NDD84 pKa = 3.5SGSSYY89 pKa = 11.01AEE91 pKa = 3.75SMALNGNSSGSDD103 pKa = 3.15LYY105 pKa = 11.08KK106 pKa = 10.87VYY108 pKa = 10.57GHH110 pKa = 6.29YY111 pKa = 10.34NQSTDD116 pKa = 2.92TLYY119 pKa = 11.53GMTEE123 pKa = 4.21VYY125 pKa = 10.4GIPGDD130 pKa = 3.55HH131 pKa = 7.55DD132 pKa = 4.56GNGDD136 pKa = 3.58VTTEE140 pKa = 3.98SSAGDD145 pKa = 3.22SGGDD149 pKa = 3.27VGPAGFGLSTQEE161 pKa = 3.55SWSIAFYY168 pKa = 9.28QVKK171 pKa = 10.57NGDD174 pKa = 3.68YY175 pKa = 10.29QDD177 pKa = 3.8YY178 pKa = 11.2SLIQISDD185 pKa = 3.86NDD187 pKa = 3.8WNIIDD192 pKa = 4.14TDD194 pKa = 3.62VGLDD198 pKa = 3.64YY199 pKa = 11.54DD200 pKa = 3.91NVSAKK205 pKa = 10.34FSYY208 pKa = 10.86QNFRR212 pKa = 11.84QIGDD216 pKa = 3.46DD217 pKa = 3.7TLPKK221 pKa = 9.87SVYY224 pKa = 9.39EE225 pKa = 3.87IKK227 pKa = 10.78VEE229 pKa = 4.06NFSKK233 pKa = 10.66FYY235 pKa = 10.91DD236 pKa = 3.66VNPGSKK242 pKa = 10.22LAIQVGAGSNGDD254 pKa = 3.66PQVGEE259 pKa = 4.02DD260 pKa = 3.33SGVVFFEE267 pKa = 4.46IPNPQIDD274 pKa = 3.56IEE276 pKa = 4.6KK277 pKa = 8.79FTKK280 pKa = 10.67DD281 pKa = 3.95MGGDD285 pKa = 3.19WQQADD290 pKa = 3.54NSADD294 pKa = 3.53PDD296 pKa = 3.89VPFFNSAGQTVEE308 pKa = 3.5WKK310 pKa = 11.25YY311 pKa = 11.11EE312 pKa = 3.77VTNNGTVPFDD322 pKa = 3.69LANVTVTDD330 pKa = 4.36DD331 pKa = 3.62KK332 pKa = 11.71GVTPVLDD339 pKa = 3.67EE340 pKa = 4.56TSDD343 pKa = 4.29DD344 pKa = 4.04GNDD347 pKa = 4.56DD348 pKa = 3.46ILSPGEE354 pKa = 3.91TWTYY358 pKa = 7.71TASGTVEE365 pKa = 4.09EE366 pKa = 4.8CPEE369 pKa = 4.17GSVYY373 pKa = 10.28TYY375 pKa = 11.32EE376 pKa = 5.03NIGTVNAISPFGEE389 pKa = 4.35VNDD392 pKa = 4.79TDD394 pKa = 3.99PSHH397 pKa = 6.71YY398 pKa = 9.97KK399 pKa = 10.03CQEE402 pKa = 3.71PTPGIDD408 pKa = 2.97IEE410 pKa = 4.85KK411 pKa = 8.48YY412 pKa = 8.99TNGEE416 pKa = 4.04NADD419 pKa = 3.82TGPGPKK425 pKa = 9.66VSNEE429 pKa = 3.95STVTWTYY436 pKa = 10.17EE437 pKa = 3.65VHH439 pKa = 6.71NNGSDD444 pKa = 3.48DD445 pKa = 3.91LQNVQVTDD453 pKa = 4.22DD454 pKa = 3.88KK455 pKa = 11.65LGTLPEE461 pKa = 4.0TAIVDD466 pKa = 3.89KK467 pKa = 11.43GDD469 pKa = 3.75GDD471 pKa = 5.06DD472 pKa = 3.94ILNIGEE478 pKa = 4.17TWVYY482 pKa = 11.23EE483 pKa = 4.09MTGTADD489 pKa = 4.22CNFTEE494 pKa = 4.79DD495 pKa = 3.79NPYY498 pKa = 11.23EE499 pKa = 4.17NTATTNGTGVITGEE513 pKa = 4.34SVEE516 pKa = 4.44DD517 pKa = 3.85TDD519 pKa = 5.09PSHH522 pKa = 6.91YY523 pKa = 10.14YY524 pKa = 10.22CKK526 pKa = 10.17PPNGVPALTPTSLMGLIGALGLIGIVGLKK555 pKa = 10.28RR556 pKa = 11.84RR557 pKa = 11.84DD558 pKa = 3.25

Molecular weight: 60.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D7E8J1|D7E8J1_METEZ Pilin_N domain-containing protein OS=Methanohalobium evestigatum (strain ATCC BAA-1072 / DSM 3721 / NBRC 107634 / OCM 161 / Z-7303) OX=644295 GN=Metev_0627 PE=4 SV=1
MM1 pKa = 7.55AKK3 pKa = 10.25GYY5 pKa = 7.33RR6 pKa = 11.84TKK8 pKa = 11.17GRR10 pKa = 11.84VTRR13 pKa = 11.84SAGRR17 pKa = 11.84FGTRR21 pKa = 11.84YY22 pKa = 9.33GRR24 pKa = 11.84KK25 pKa = 9.16DD26 pKa = 3.08RR27 pKa = 11.84KK28 pKa = 10.24LIANIEE34 pKa = 4.09EE35 pKa = 4.22QMHH38 pKa = 5.98KK39 pKa = 10.33KK40 pKa = 9.52HH41 pKa = 6.38RR42 pKa = 11.84CSNCARR48 pKa = 11.84VSVRR52 pKa = 11.84RR53 pKa = 11.84TGTGIWEE60 pKa = 4.34CTKK63 pKa = 10.56CGHH66 pKa = 6.1TFAGGTYY73 pKa = 9.32TPQTSVGKK81 pKa = 7.63TVPRR85 pKa = 11.84AIRR88 pKa = 11.84KK89 pKa = 9.27AIEE92 pKa = 4.2EE93 pKa = 4.11EE94 pKa = 4.52VEE96 pKa = 3.91

Molecular weight: 10.82 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2250

0

2250

666791

31

2559

296.4

33.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.654 ± 0.051

1.14 ± 0.022

6.477 ± 0.053

7.358 ± 0.057

3.863 ± 0.038

6.723 ± 0.05

1.83 ± 0.021

8.446 ± 0.056

7.253 ± 0.066

8.466 ± 0.063

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.524 ± 0.029

5.926 ± 0.07

3.597 ± 0.026

2.8 ± 0.026

3.814 ± 0.041

6.937 ± 0.048

5.491 ± 0.054

6.913 ± 0.038

0.849 ± 0.018

3.936 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski