Amino acid dipepetide frequency for Methanohalobium evestigatum (strain ATCC BAA-1072 / DSM 3721 / NBRC 107634 / OCM 161 / Z-7303)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.757AlaAla: 3.757 ± 0.099
0.681AlaCys: 0.681 ± 0.032
3.556AlaAsp: 3.556 ± 0.088
3.913AlaGlu: 3.913 ± 0.085
2.406AlaPhe: 2.406 ± 0.07
4.369AlaGly: 4.369 ± 0.091
0.973AlaHis: 0.973 ± 0.039
4.739AlaIle: 4.739 ± 0.115
3.731AlaLys: 3.731 ± 0.076
5.146AlaLeu: 5.146 ± 0.12
1.434AlaMet: 1.434 ± 0.056
2.602AlaAsn: 2.602 ± 0.069
1.672AlaPro: 1.672 ± 0.053
1.579AlaGln: 1.579 ± 0.05
2.346AlaArg: 2.346 ± 0.061
3.827AlaSer: 3.827 ± 0.067
2.927AlaThr: 2.927 ± 0.074
4.564AlaVal: 4.564 ± 0.091
0.394AlaTrp: 0.394 ± 0.029
1.929AlaTyr: 1.929 ± 0.058
0.0AlaXaa: 0.0 ± 0.0
Cys
0.585CysAla: 0.585 ± 0.029
0.177CysCys: 0.177 ± 0.017
0.67CysAsp: 0.67 ± 0.034
0.784CysGlu: 0.784 ± 0.033
0.301CysPhe: 0.301 ± 0.021
1.261CysGly: 1.261 ± 0.048
0.301CysHis: 0.301 ± 0.025
0.843CysIle: 0.843 ± 0.037
0.718CysLys: 0.718 ± 0.038
0.718CysLeu: 0.718 ± 0.036
0.259CysMet: 0.259 ± 0.02
0.642CysAsn: 0.642 ± 0.029
0.756CysPro: 0.756 ± 0.043
0.246CysGln: 0.246 ± 0.02
0.513CysArg: 0.513 ± 0.028
0.832CysSer: 0.832 ± 0.036
0.612CysThr: 0.612 ± 0.033
0.688CysVal: 0.688 ± 0.031
0.067CysTrp: 0.067 ± 0.01
0.42CysTyr: 0.42 ± 0.026
0.0CysXaa: 0.0 ± 0.0
Asp
3.602AspAla: 3.602 ± 0.088
0.619AspCys: 0.619 ± 0.035
4.009AspAsp: 4.009 ± 0.086
5.405AspGlu: 5.405 ± 0.103
2.909AspPhe: 2.909 ± 0.07
4.111AspGly: 4.111 ± 0.117
0.856AspHis: 0.856 ± 0.035
6.545AspIle: 6.545 ± 0.1
4.636AspLys: 4.636 ± 0.094
5.006AspLeu: 5.006 ± 0.076
1.767AspMet: 1.767 ± 0.048
3.893AspAsn: 3.893 ± 0.083
2.046AspPro: 2.046 ± 0.06
1.125AspGln: 1.125 ± 0.045
2.319AspArg: 2.319 ± 0.067
4.027AspSer: 4.027 ± 0.095
3.604AspThr: 3.604 ± 0.097
5.072AspVal: 5.072 ± 0.101
0.544AspTrp: 0.544 ± 0.027
2.68AspTyr: 2.68 ± 0.061
0.0AspXaa: 0.0 ± 0.0
Glu
3.517GluAla: 3.517 ± 0.078
0.789GluCys: 0.789 ± 0.03
4.385GluAsp: 4.385 ± 0.081
5.206GluGlu: 5.206 ± 0.129
2.812GluPhe: 2.812 ± 0.068
3.907GluGly: 3.907 ± 0.071
1.341GluHis: 1.341 ± 0.051
6.344GluIle: 6.344 ± 0.11
6.228GluLys: 6.228 ± 0.123
6.783GluLeu: 6.783 ± 0.097
1.998GluMet: 1.998 ± 0.053
5.454GluAsn: 5.454 ± 0.101
2.406GluPro: 2.406 ± 0.075
2.314GluGln: 2.314 ± 0.062
2.786GluArg: 2.786 ± 0.065
4.934GluSer: 4.934 ± 0.101
4.006GluThr: 4.006 ± 0.086
4.354GluVal: 4.354 ± 0.09
0.72GluTrp: 0.72 ± 0.036
3.296GluTyr: 3.296 ± 0.066
0.0GluXaa: 0.0 ± 0.0
Phe
2.434PheAla: 2.434 ± 0.074
0.465PheCys: 0.465 ± 0.03
2.74PheAsp: 2.74 ± 0.063
2.953PheGlu: 2.953 ± 0.071
1.561PhePhe: 1.561 ± 0.065
2.885PheGly: 2.885 ± 0.072
0.583PheHis: 0.583 ± 0.03
3.062PheIle: 3.062 ± 0.089
2.604PheLys: 2.604 ± 0.066
3.262PheLeu: 3.262 ± 0.097
0.948PheMet: 0.948 ± 0.039
2.001PheAsn: 2.001 ± 0.058
1.153PhePro: 1.153 ± 0.047
0.888PheGln: 0.888 ± 0.035
1.344PheArg: 1.344 ± 0.046
2.855PheSer: 2.855 ± 0.071
2.296PheThr: 2.296 ± 0.079
2.783PheVal: 2.783 ± 0.064
0.349PheTrp: 0.349 ± 0.026
1.464PheTyr: 1.464 ± 0.052
0.0PheXaa: 0.0 ± 0.0
Gly
4.064GlyAla: 4.064 ± 0.094
0.99GlyCys: 0.99 ± 0.047
3.884GlyAsp: 3.884 ± 0.089
4.442GlyGlu: 4.442 ± 0.089
3.025GlyPhe: 3.025 ± 0.079
4.303GlyGly: 4.303 ± 0.107
1.233GlyHis: 1.233 ± 0.042
6.483GlyIle: 6.483 ± 0.12
4.781GlyLys: 4.781 ± 0.094
5.535GlyLeu: 5.535 ± 0.112
1.966GlyMet: 1.966 ± 0.058
3.542GlyAsn: 3.542 ± 0.099
1.714GlyPro: 1.714 ± 0.057
1.555GlyGln: 1.555 ± 0.054
2.413GlyArg: 2.413 ± 0.071
4.31GlySer: 4.31 ± 0.092
4.111GlyThr: 4.111 ± 0.1
4.862GlyVal: 4.862 ± 0.098
0.711GlyTrp: 0.711 ± 0.031
3.307GlyTyr: 3.307 ± 0.078
0.0GlyXaa: 0.0 ± 0.0
His
1.0HisAla: 1.0 ± 0.041
0.325HisCys: 0.325 ± 0.02
1.051HisAsp: 1.051 ± 0.041
1.317HisGlu: 1.317 ± 0.052
0.673HisPhe: 0.673 ± 0.032
1.321HisGly: 1.321 ± 0.046
0.406HisHis: 0.406 ± 0.026
1.639HisIle: 1.639 ± 0.055
1.095HisLys: 1.095 ± 0.039
1.731HisLeu: 1.731 ± 0.049
0.447HisMet: 0.447 ± 0.024
0.91HisAsn: 0.91 ± 0.035
1.062HisPro: 1.062 ± 0.038
0.52HisGln: 0.52 ± 0.03
0.817HisArg: 0.817 ± 0.039
1.099HisSer: 1.099 ± 0.04
0.87HisThr: 0.87 ± 0.034
1.173HisVal: 1.173 ± 0.042
0.177HisTrp: 0.177 ± 0.015
0.663HisTyr: 0.663 ± 0.03
0.0HisXaa: 0.0 ± 0.0
Ile
5.348IleAla: 5.348 ± 0.102
0.897IleCys: 0.897 ± 0.04
6.045IleAsp: 6.045 ± 0.11
6.191IleGlu: 6.191 ± 0.111
3.127IlePhe: 3.127 ± 0.1
5.816IleGly: 5.816 ± 0.116
1.62IleHis: 1.62 ± 0.046
6.645IleIle: 6.645 ± 0.14
6.002IleLys: 6.002 ± 0.103
7.101IleLeu: 7.101 ± 0.115
1.833IleMet: 1.833 ± 0.058
5.08IleAsn: 5.08 ± 0.102
3.476IlePro: 3.476 ± 0.085
2.197IleGln: 2.197 ± 0.065
3.107IleArg: 3.107 ± 0.076
6.17IleSer: 6.17 ± 0.101
5.057IleThr: 5.057 ± 0.087
5.445IleVal: 5.445 ± 0.109
0.598IleTrp: 0.598 ± 0.03
2.701IleTyr: 2.701 ± 0.067
0.0IleXaa: 0.0 ± 0.0
Lys
3.647LysAla: 3.647 ± 0.085
0.792LysCys: 0.792 ± 0.037
4.409LysAsp: 4.409 ± 0.09
5.182LysGlu: 5.182 ± 0.099
2.314LysPhe: 2.314 ± 0.066
4.127LysGly: 4.127 ± 0.077
1.512LysHis: 1.512 ± 0.054
5.879LysIle: 5.879 ± 0.116
5.94LysLys: 5.94 ± 0.113
6.113LysLeu: 6.113 ± 0.106
1.878LysMet: 1.878 ± 0.047
5.224LysAsn: 5.224 ± 0.1
2.458LysPro: 2.458 ± 0.059
2.659LysGln: 2.659 ± 0.072
3.145LysArg: 3.145 ± 0.077
5.318LysSer: 5.318 ± 0.101
3.892LysThr: 3.892 ± 0.086
4.219LysVal: 4.219 ± 0.084
0.613LysTrp: 0.613 ± 0.034
3.212LysTyr: 3.212 ± 0.074
0.0LysXaa: 0.0 ± 0.0
Leu
4.894LeuAla: 4.894 ± 0.097
0.771LeuCys: 0.771 ± 0.039
5.795LeuAsp: 5.795 ± 0.103
6.347LeuGlu: 6.347 ± 0.11
3.061LeuPhe: 3.061 ± 0.095
5.616LeuGly: 5.616 ± 0.107
1.432LeuHis: 1.432 ± 0.051
6.767LeuIle: 6.767 ± 0.123
7.028LeuLys: 7.028 ± 0.11
6.963LeuLeu: 6.963 ± 0.142
2.245LeuMet: 2.245 ± 0.063
5.222LeuAsn: 5.222 ± 0.105
3.295LeuPro: 3.295 ± 0.076
2.389LeuGln: 2.389 ± 0.058
2.999LeuArg: 2.999 ± 0.08
6.344LeuSer: 6.344 ± 0.11
4.435LeuThr: 4.435 ± 0.089
5.399LeuVal: 5.399 ± 0.108
0.631LeuTrp: 0.631 ± 0.033
3.028LeuTyr: 3.028 ± 0.077
0.0LeuXaa: 0.0 ± 0.0
Met
1.606MetAla: 1.606 ± 0.047
0.273MetCys: 0.273 ± 0.021
1.944MetAsp: 1.944 ± 0.06
1.836MetGlu: 1.836 ± 0.057
0.864MetPhe: 0.864 ± 0.04
1.912MetGly: 1.912 ± 0.055
0.439MetHis: 0.439 ± 0.024
1.75MetIle: 1.75 ± 0.051
1.902MetLys: 1.902 ± 0.054
2.122MetLeu: 2.122 ± 0.057
0.708MetMet: 0.708 ± 0.044
1.458MetAsn: 1.458 ± 0.045
0.802MetPro: 0.802 ± 0.04
0.696MetGln: 0.696 ± 0.035
0.925MetArg: 0.925 ± 0.034
1.722MetSer: 1.722 ± 0.048
1.299MetThr: 1.299 ± 0.049
1.957MetVal: 1.957 ± 0.054
0.165MetTrp: 0.165 ± 0.015
0.859MetTyr: 0.859 ± 0.034
0.0MetXaa: 0.0 ± 0.0
Asn
3.058AsnAla: 3.058 ± 0.065
0.646AsnCys: 0.646 ± 0.037
3.37AsnAsp: 3.37 ± 0.087
3.886AsnGlu: 3.886 ± 0.079
2.061AsnPhe: 2.061 ± 0.055
3.499AsnGly: 3.499 ± 0.094
1.12AsnHis: 1.12 ± 0.039
5.67AsnIle: 5.67 ± 0.106
4.226AsnLys: 4.226 ± 0.104
5.282AsnLeu: 5.282 ± 0.102
1.299AsnMet: 1.299 ± 0.049
4.097AsnAsn: 4.097 ± 0.117
2.798AsnPro: 2.798 ± 0.07
1.888AsnGln: 1.888 ± 0.063
2.218AsnArg: 2.218 ± 0.056
3.862AsnSer: 3.862 ± 0.11
3.377AsnThr: 3.377 ± 0.084
4.051AsnVal: 4.051 ± 0.098
0.532AsnTrp: 0.532 ± 0.025
2.316AsnTyr: 2.316 ± 0.069
0.0AsnXaa: 0.0 ± 0.0
Pro
1.809ProAla: 1.809 ± 0.055
0.421ProCys: 0.421 ± 0.026
2.894ProAsp: 2.894 ± 0.064
3.989ProGlu: 3.989 ± 0.072
1.474ProPhe: 1.474 ± 0.057
2.364ProGly: 2.364 ± 0.075
0.75ProHis: 0.75 ± 0.038
2.494ProIle: 2.494 ± 0.062
2.07ProLys: 2.07 ± 0.054
2.893ProLeu: 2.893 ± 0.074
0.78ProMet: 0.78 ± 0.032
1.534ProAsn: 1.534 ± 0.046
1.131ProPro: 1.131 ± 0.049
1.003ProGln: 1.003 ± 0.038
1.158ProArg: 1.158 ± 0.046
2.41ProSer: 2.41 ± 0.062
1.684ProThr: 1.684 ± 0.049
3.404ProVal: 3.404 ± 0.083
0.27ProTrp: 0.27 ± 0.023
1.441ProTyr: 1.441 ± 0.054
0.0ProXaa: 0.0 ± 0.0
Gln
1.33GlnAla: 1.33 ± 0.047
0.34GlnCys: 0.34 ± 0.023
1.611GlnAsp: 1.611 ± 0.052
1.939GlnGlu: 1.939 ± 0.052
0.99GlnPhe: 0.99 ± 0.041
1.462GlnGly: 1.462 ± 0.043
0.499GlnHis: 0.499 ± 0.03
2.521GlnIle: 2.521 ± 0.065
2.475GlnLys: 2.475 ± 0.061
2.52GlnLeu: 2.52 ± 0.061
0.879GlnMet: 0.879 ± 0.033
1.834GlnAsn: 1.834 ± 0.064
0.862GlnPro: 0.862 ± 0.038
0.967GlnGln: 0.967 ± 0.048
1.135GlnArg: 1.135 ± 0.046
1.917GlnSer: 1.917 ± 0.059
1.498GlnThr: 1.498 ± 0.05
1.786GlnVal: 1.786 ± 0.065
0.294GlnTrp: 0.294 ± 0.02
1.137GlnTyr: 1.137 ± 0.044
0.0GlnXaa: 0.0 ± 0.0
Arg
2.148ArgAla: 2.148 ± 0.063
0.489ArgCys: 0.489 ± 0.029
2.458ArgAsp: 2.458 ± 0.07
2.903ArgGlu: 2.903 ± 0.08
1.693ArgPhe: 1.693 ± 0.052
2.394ArgGly: 2.394 ± 0.051
0.631ArgHis: 0.631 ± 0.035
3.364ArgIle: 3.364 ± 0.074
3.047ArgLys: 3.047 ± 0.077
3.37ArgLeu: 3.37 ± 0.081
1.018ArgMet: 1.018 ± 0.038
2.07ArgAsn: 2.07 ± 0.06
1.191ArgPro: 1.191 ± 0.044
1.09ArgGln: 1.09 ± 0.044
1.632ArgArg: 1.632 ± 0.053
2.185ArgSer: 2.185 ± 0.058
1.938ArgThr: 1.938 ± 0.056
2.467ArgVal: 2.467 ± 0.067
0.394ArgTrp: 0.394 ± 0.026
1.657ArgTyr: 1.657 ± 0.05
0.0ArgXaa: 0.0 ± 0.0
Ser
3.904SerAla: 3.904 ± 0.09
0.66SerCys: 0.66 ± 0.03
4.559SerAsp: 4.559 ± 0.097
5.279SerGlu: 5.279 ± 0.106
2.632SerPhe: 2.632 ± 0.075
5.597SerGly: 5.597 ± 0.098
1.254SerHis: 1.254 ± 0.046
5.805SerIle: 5.805 ± 0.112
5.126SerLys: 5.126 ± 0.09
5.405SerLeu: 5.405 ± 0.104
1.809SerMet: 1.809 ± 0.047
3.746SerAsn: 3.746 ± 0.09
2.277SerPro: 2.277 ± 0.059
2.122SerGln: 2.122 ± 0.056
2.782SerArg: 2.782 ± 0.063
5.125SerSer: 5.125 ± 0.119
3.626SerThr: 3.626 ± 0.077
4.63SerVal: 4.63 ± 0.099
0.555SerTrp: 0.555 ± 0.033
2.479SerTyr: 2.479 ± 0.057
0.0SerXaa: 0.0 ± 0.0
Thr
3.286ThrAla: 3.286 ± 0.079
0.609ThrCys: 0.609 ± 0.028
3.448ThrAsp: 3.448 ± 0.087
3.701ThrGlu: 3.701 ± 0.088
1.998ThrPhe: 1.998 ± 0.055
4.804ThrGly: 4.804 ± 0.097
1.029ThrHis: 1.029 ± 0.039
4.561ThrIle: 4.561 ± 0.101
3.23ThrLys: 3.23 ± 0.077
4.742ThrLeu: 4.742 ± 0.099
1.143ThrMet: 1.143 ± 0.039
2.792ThrAsn: 2.792 ± 0.088
2.325ThrPro: 2.325 ± 0.057
1.479ThrGln: 1.479 ± 0.046
2.064ThrArg: 2.064 ± 0.06
3.992ThrSer: 3.992 ± 0.083
3.107ThrThr: 3.107 ± 0.086
4.163ThrVal: 4.163 ± 0.101
0.396ThrTrp: 0.396 ± 0.026
2.046ThrTyr: 2.046 ± 0.058
0.0ThrXaa: 0.0 ± 0.0
Val
4.108ValAla: 4.108 ± 0.081
0.865ValCys: 0.865 ± 0.039
5.074ValAsp: 5.074 ± 0.093
5.009ValGlu: 5.009 ± 0.109
2.774ValPhe: 2.774 ± 0.082
4.345ValGly: 4.345 ± 0.108
1.266ValHis: 1.266 ± 0.039
5.637ValIle: 5.637 ± 0.112
4.454ValLys: 4.454 ± 0.089
5.666ValLeu: 5.666 ± 0.103
1.674ValMet: 1.674 ± 0.054
3.926ValAsn: 3.926 ± 0.088
2.686ValPro: 2.686 ± 0.069
1.708ValGln: 1.708 ± 0.047
2.436ValArg: 2.436 ± 0.065
5.18ValSer: 5.18 ± 0.089
3.973ValThr: 3.973 ± 0.106
5.225ValVal: 5.225 ± 0.111
0.513ValTrp: 0.513 ± 0.032
2.616ValTyr: 2.616 ± 0.063
0.0ValXaa: 0.0 ± 0.0
Trp
0.45TrpAla: 0.45 ± 0.029
0.126TrpCys: 0.126 ± 0.014
0.571TrpAsp: 0.571 ± 0.033
0.528TrpGlu: 0.528 ± 0.033
0.385TrpPhe: 0.385 ± 0.026
0.51TrpGly: 0.51 ± 0.031
0.147TrpHis: 0.147 ± 0.015
0.762TrpIle: 0.762 ± 0.038
0.64TrpLys: 0.64 ± 0.033
0.733TrpLeu: 0.733 ± 0.037
0.255TrpMet: 0.255 ± 0.018
0.582TrpAsn: 0.582 ± 0.036
0.19TrpPro: 0.19 ± 0.017
0.252TrpGln: 0.252 ± 0.021
0.264TrpArg: 0.264 ± 0.021
0.553TrpSer: 0.553 ± 0.028
0.417TrpThr: 0.417 ± 0.028
0.592TrpVal: 0.592 ± 0.029
0.148TrpTrp: 0.148 ± 0.015
0.381TrpTyr: 0.381 ± 0.026
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.996TyrAla: 1.996 ± 0.058
0.46TyrCys: 0.46 ± 0.028
2.301TyrAsp: 2.301 ± 0.065
2.77TyrGlu: 2.77 ± 0.068
1.57TyrPhe: 1.57 ± 0.05
2.629TyrGly: 2.629 ± 0.065
0.904TyrHis: 0.904 ± 0.038
2.951TyrIle: 2.951 ± 0.074
2.599TyrLys: 2.599 ± 0.072
3.571TyrLeu: 3.571 ± 0.078
0.9TyrMet: 0.9 ± 0.037
2.647TyrAsn: 2.647 ± 0.069
1.674TyrPro: 1.674 ± 0.051
1.318TyrGln: 1.318 ± 0.048
1.737TyrArg: 1.737 ± 0.051
2.708TyrSer: 2.708 ± 0.061
2.185TyrThr: 2.185 ± 0.059
2.295TyrVal: 2.295 ± 0.06
0.415TyrTrp: 0.415 ± 0.026
1.728TyrTyr: 1.728 ± 0.062
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2250 proteins (666791 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski