Fervidicoccus fontis (strain DSM 19380 / JCM 18336 / VKM B-2539 / Kam940)

Taxonomy: cellular organisms; Archaea; TACK group; Crenarchaeota; Thermoprotei; Fervidicoccales; Fervidicoccaceae; Fervidicoccus; Fervidicoccus fontis

Average proteome isoelectric point is 7.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1384 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I0A1T3|I0A1T3_FERFK V-type ATP synthase alpha chain OS=Fervidicoccus fontis (strain DSM 19380 / JCM 18336 / VKM B-2539 / Kam940) OX=1163730 GN=atpA PE=3 SV=1
MM1 pKa = 7.78IFDD4 pKa = 4.74DD5 pKa = 4.46EE6 pKa = 5.67KK7 pKa = 10.69IDD9 pKa = 4.64EE10 pKa = 4.45ISPYY14 pKa = 8.23TKK16 pKa = 9.7WYY18 pKa = 9.05ILGIILGVAAGLSALAFYY36 pKa = 10.44FSLL39 pKa = 4.76

Molecular weight: 4.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I0A2R8|I0A2R8_FERFK Component HyfF of membrane-bound [Ni Fe]-hydrogenase OS=Fervidicoccus fontis (strain DSM 19380 / JCM 18336 / VKM B-2539 / Kam940) OX=1163730 GN=FFONT_1287 PE=4 SV=1
MM1 pKa = 7.41AHH3 pKa = 6.33YY4 pKa = 10.27KK5 pKa = 10.14PLARR9 pKa = 11.84KK10 pKa = 9.11LRR12 pKa = 11.84LSKK15 pKa = 10.56ALKK18 pKa = 9.91SNSPIPVWVAVKK30 pKa = 8.99TKK32 pKa = 10.49RR33 pKa = 11.84KK34 pKa = 9.62VRR36 pKa = 11.84FNFKK40 pKa = 9.79RR41 pKa = 11.84RR42 pKa = 11.84FWRR45 pKa = 11.84RR46 pKa = 11.84NKK48 pKa = 10.68LKK50 pKa = 10.83VV51 pKa = 3.09

Molecular weight: 6.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1384

0

1384

381356

37

2036

275.5

31.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.091 ± 0.055

0.717 ± 0.024

4.441 ± 0.047

7.753 ± 0.097

4.417 ± 0.054

6.67 ± 0.05

1.305 ± 0.025

9.677 ± 0.074

8.223 ± 0.098

10.039 ± 0.071

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.428 ± 0.027

4.396 ± 0.058

3.896 ± 0.039

1.924 ± 0.036

4.416 ± 0.058

7.464 ± 0.078

4.281 ± 0.049

6.702 ± 0.042

0.953 ± 0.024

4.206 ± 0.049
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski