Acinetobacter larvae
Average proteome isoelectric point is 6.58
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3108 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1B2M2T1|A0A1B2M2T1_9GAMM RNA pyrophosphohydrolase OS=Acinetobacter larvae OX=1789224 GN=rppH PE=3 SV=1
MM1 pKa = 7.74 LFDD4 pKa = 4.49 LFKK7 pKa = 11.12 NSSLSKK13 pKa = 8.19 YY14 pKa = 9.91 IEE16 pKa = 4.18 YY17 pKa = 10.45 AVCDD21 pKa = 4.36 DD22 pKa = 4.39 DD23 pKa = 4.38 CLEE26 pKa = 4.1 VFFIDD31 pKa = 4.26 SDD33 pKa = 3.89 EE34 pKa = 6.13 LFILWNSNLDD44 pKa = 3.27 INYY47 pKa = 8.19 ATIISNNIRR56 pKa = 11.84 LPSLIIFF63 pKa = 4.68
Molecular weight: 7.39 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.758
IPC2_protein 3.77
IPC_protein 3.668
Toseland 3.465
ProMoST 3.884
Dawson 3.681
Bjellqvist 3.859
Wikipedia 3.681
Rodwell 3.503
Grimsley 3.389
Solomon 3.643
Lehninger 3.605
Nozaki 3.834
DTASelect 4.05
Thurlkill 3.554
EMBOSS 3.681
Sillero 3.795
Patrickios 0.604
IPC_peptide 3.643
IPC2_peptide 3.757
IPC2.peptide.svr19 3.747
Protein with the highest isoelectric point:
>tr|A0A1B2M3R2|A0A1B2M3R2_9GAMM Transposase OS=Acinetobacter larvae OX=1789224 GN=BFG52_03195 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.55 RR3 pKa = 11.84 TFQPSEE9 pKa = 3.97 LKK11 pKa = 10.13 RR12 pKa = 11.84 KK13 pKa = 8.98 RR14 pKa = 11.84 VHH16 pKa = 6.36 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.4 AGRR28 pKa = 11.84 QVLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.18 GRR39 pKa = 11.84 HH40 pKa = 5.1 SLTVV44 pKa = 3.06
Molecular weight: 5.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.402
IPC2_protein 10.862
IPC_protein 12.442
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.31
Grimsley 12.647
Solomon 13.1
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.047
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.077
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3108
0
3108
1021974
33
5402
328.8
36.65
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.694 ± 0.047
1.002 ± 0.016
5.251 ± 0.047
5.069 ± 0.041
4.013 ± 0.031
6.309 ± 0.069
2.621 ± 0.028
6.729 ± 0.042
4.87 ± 0.047
10.679 ± 0.077
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.349 ± 0.022
4.178 ± 0.045
3.883 ± 0.037
6.536 ± 0.079
4.536 ± 0.043
6.152 ± 0.036
5.008 ± 0.06
6.385 ± 0.052
1.301 ± 0.021
3.433 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here