Enterobacteriaceae bacterium 4M9

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; unclassified Enterobacteriaceae

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4055 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6B2FKK9|A0A6B2FKK9_9ENTR Flagellar biosynthesis protein FlgN OS=Enterobacteriaceae bacterium 4M9 OX=2699502 GN=GWD52_01605 PE=3 SV=1
MM1 pKa = 7.69SDD3 pKa = 2.89VTLFSHH9 pKa = 6.99RR10 pKa = 11.84GSNPYY15 pKa = 9.38PDD17 pKa = 4.24HH18 pKa = 6.39TLEE21 pKa = 4.86AYY23 pKa = 9.93NAGIDD28 pKa = 3.34WGADD32 pKa = 3.73FIEE35 pKa = 4.99PDD37 pKa = 4.3LYY39 pKa = 9.99ITSDD43 pKa = 3.49GVLVAAHH50 pKa = 7.37DD51 pKa = 4.24DD52 pKa = 3.48MGFANMTYY60 pKa = 10.62AQALAQNSSLMRR72 pKa = 11.84FDD74 pKa = 5.02QIIDD78 pKa = 3.71LVNTRR83 pKa = 11.84SVEE86 pKa = 3.88TGRR89 pKa = 11.84QIGITYY95 pKa = 8.8EE96 pKa = 3.96IKK98 pKa = 9.83NTGNDD103 pKa = 3.53ALTLQAAEE111 pKa = 5.22LSVQMLIDD119 pKa = 3.73KK120 pKa = 10.57GFTNPDD126 pKa = 3.65LIFINSASSDD136 pKa = 3.43TLRR139 pKa = 11.84YY140 pKa = 10.36LNDD143 pKa = 3.12TVMPQSGVDD152 pKa = 3.84FPLIRR157 pKa = 11.84LLSVQEE163 pKa = 4.11SLALKK168 pKa = 10.24YY169 pKa = 10.86GQFNPASVTDD179 pKa = 3.96FADD182 pKa = 5.75GIAVPAYY189 pKa = 8.85IKK191 pKa = 10.95APGVPFSPTINIVDD205 pKa = 3.74SSLVAKK211 pKa = 9.54VHH213 pKa = 5.37AQGLQIHH220 pKa = 5.63TWEE223 pKa = 4.11ALGNEE228 pKa = 4.29FTDD231 pKa = 4.19ARR233 pKa = 11.84LAYY236 pKa = 10.11LDD238 pKa = 3.75KK239 pKa = 10.87TGVDD243 pKa = 4.67AIYY246 pKa = 10.78SDD248 pKa = 5.12YY249 pKa = 10.86IDD251 pKa = 4.13SSTIGLNKK259 pKa = 9.93ADD261 pKa = 3.88GVNTVLGDD269 pKa = 3.89TNSTTLTGTSGDD281 pKa = 3.75DD282 pKa = 3.67VIYY285 pKa = 10.95AMQGDD290 pKa = 4.49DD291 pKa = 3.05IVYY294 pKa = 10.12GKK296 pKa = 10.61GGNDD300 pKa = 3.12VLYY303 pKa = 11.0GDD305 pKa = 5.21GGNDD309 pKa = 3.29TLIAGNGNDD318 pKa = 3.77ILVGGAGNDD327 pKa = 3.39YY328 pKa = 10.71LYY330 pKa = 11.19AGLADD335 pKa = 4.24SLQSQNVLDD344 pKa = 4.32GGVGNDD350 pKa = 3.51VIVTSGTDD358 pKa = 3.25TILYY362 pKa = 7.04GTGDD366 pKa = 4.29GIDD369 pKa = 3.91LLVTNNTSTLQFEE382 pKa = 5.1DD383 pKa = 3.57IARR386 pKa = 11.84QDD388 pKa = 3.26VTFQQYY394 pKa = 11.5NNDD397 pKa = 3.43LVISINNGGALIVQNGASDD416 pKa = 3.85AGSLPGSLVFSDD428 pKa = 6.34GILQTADD435 pKa = 2.91IQTSGSVSPEE445 pKa = 3.87TIAALPDD452 pKa = 3.51LQALLNSGNQLASADD467 pKa = 3.53LWVAA471 pKa = 3.72

Molecular weight: 49.65 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6B2FN82|A0A6B2FN82_9ENTR Uncharacterized MFS-type transporter GWD52_15040 OS=Enterobacteriaceae bacterium 4M9 OX=2699502 GN=GWD52_15040 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.6RR12 pKa = 11.84NRR14 pKa = 11.84SHH16 pKa = 7.16GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.4NGRR28 pKa = 11.84QVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.22GRR39 pKa = 11.84ARR41 pKa = 11.84LTVSKK46 pKa = 10.99

Molecular weight: 5.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4055

0

4055

1319694

20

7194

325.4

35.86

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.071 ± 0.044

1.067 ± 0.017

5.309 ± 0.052

5.581 ± 0.04

3.717 ± 0.033

7.64 ± 0.061

2.219 ± 0.019

5.428 ± 0.032

3.855 ± 0.041

10.816 ± 0.073

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.648 ± 0.028

3.767 ± 0.04

4.471 ± 0.033

4.482 ± 0.042

5.898 ± 0.06

6.069 ± 0.036

5.538 ± 0.089

7.179 ± 0.04

1.549 ± 0.017

2.696 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski