Amino acid dipepetide frequency for Enterobacteriaceae bacterium 4M9

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	10.58AlaAla: 10.58 ± 0.135	1.193AlaCys: 1.193 ± 0.033	5.045AlaAsp: 5.045 ± 0.082	5.364AlaGlu: 5.364 ± 0.083	3.562AlaPhe: 3.562 ± 0.06	8.18AlaGly: 8.18 ± 0.149	2.05AlaHis: 2.05 ± 0.037	5.527AlaIle: 5.527 ± 0.072	3.405AlaLys: 3.405 ± 0.065	12.881AlaLeu: 12.881 ± 0.142	2.83AlaMet: 2.83 ± 0.054	3.02AlaAsn: 3.02 ± 0.05	4.107AlaPro: 4.107 ± 0.078	5.33AlaGln: 5.33 ± 0.102	6.212AlaArg: 6.212 ± 0.107	5.894AlaSer: 5.894 ± 0.083	5.041AlaThr: 5.041 ± 0.086	6.885AlaVal: 6.885 ± 0.081	1.72AlaTrp: 1.72 ± 0.036	1.88AlaTyr: 1.88 ± 0.037	0.0AlaXaa: 0.0 ± 0.0
Cys	1.071CysAla: 1.071 ± 0.033	0.167CysCys: 0.167 ± 0.011	0.608CysAsp: 0.608 ± 0.023	0.638CysGlu: 0.638 ± 0.024	0.413CysPhe: 0.413 ± 0.019	1.076CysGly: 1.076 ± 0.032	0.319CysHis: 0.319 ± 0.02	0.519CysIle: 0.519 ± 0.021	0.27CysLys: 0.27 ± 0.016	0.948CysLeu: 0.948 ± 0.026	0.227CysMet: 0.227 ± 0.012	0.339CysAsn: 0.339 ± 0.017	0.498CysPro: 0.498 ± 0.022	0.426CysGln: 0.426 ± 0.019	0.715CysArg: 0.715 ± 0.027	0.648CysSer: 0.648 ± 0.021	0.498CysThr: 0.498 ± 0.02	0.784CysVal: 0.784 ± 0.029	0.181CysTrp: 0.181 ± 0.012	0.324CysTyr: 0.324 ± 0.015	0.0CysXaa: 0.0 ± 0.0
Asp	5.551AspAla: 5.551 ± 0.093	0.533AspCys: 0.533 ± 0.023	3.252AspAsp: 3.252 ± 0.11	3.706AspGlu: 3.706 ± 0.064	2.072AspPhe: 2.072 ± 0.041	4.287AspGly: 4.287 ± 0.118	0.835AspHis: 0.835 ± 0.025	3.592AspIle: 3.592 ± 0.058	2.477AspLys: 2.477 ± 0.047	4.292AspLeu: 4.292 ± 0.059	1.412AspMet: 1.412 ± 0.037	2.384AspAsn: 2.384 ± 0.065	2.134AspPro: 2.134 ± 0.05	1.319AspGln: 1.319 ± 0.033	2.507AspArg: 2.507 ± 0.054	3.11AspSer: 3.11 ± 0.054	2.812AspThr: 2.812 ± 0.101	4.037AspVal: 4.037 ± 0.067	0.881AspTrp: 0.881 ± 0.028	1.898AspTyr: 1.898 ± 0.043	0.0AspXaa: 0.0 ± 0.0
Glu	5.371GluAla: 5.371 ± 0.077	0.469GluCys: 0.469 ± 0.022	2.423GluAsp: 2.423 ± 0.052	3.123GluGlu: 3.123 ± 0.071	1.763GluPhe: 1.763 ± 0.038	3.616GluGly: 3.616 ± 0.059	1.488GluHis: 1.488 ± 0.039	3.079GluIle: 3.079 ± 0.058	3.032GluLys: 3.032 ± 0.06	5.988GluLeu: 5.988 ± 0.079	1.71GluMet: 1.71 ± 0.036	2.26GluAsn: 2.26 ± 0.038	2.267GluPro: 2.267 ± 0.049	3.494GluGln: 3.494 ± 0.081	4.016GluArg: 4.016 ± 0.074	2.956GluSer: 2.956 ± 0.058	2.874GluThr: 2.874 ± 0.07	3.765GluVal: 3.765 ± 0.068	0.731GluTrp: 0.731 ± 0.025	1.387GluTyr: 1.387 ± 0.037	0.0GluXaa: 0.0 ± 0.0
Phe	3.658PheAla: 3.658 ± 0.062	0.527PheCys: 0.527 ± 0.019	2.256PheAsp: 2.256 ± 0.045	1.794PheGlu: 1.794 ± 0.042	1.595PhePhe: 1.595 ± 0.042	3.017PheGly: 3.017 ± 0.057	0.78PheHis: 0.78 ± 0.027	2.382PheIle: 2.382 ± 0.055	1.1PheLys: 1.1 ± 0.032	3.197PheLeu: 3.197 ± 0.063	0.965PheMet: 0.965 ± 0.032	1.659PheAsn: 1.659 ± 0.039	1.434PhePro: 1.434 ± 0.039	1.071PheGln: 1.071 ± 0.03	1.844PheArg: 1.844 ± 0.038	3.168PheSer: 3.168 ± 0.056	2.531PheThr: 2.531 ± 0.079	2.398PheVal: 2.398 ± 0.046	0.646PheTrp: 0.646 ± 0.025	1.15PheTyr: 1.15 ± 0.028	0.0PheXaa: 0.0 ± 0.0
Gly	6.823GlyAla: 6.823 ± 0.096	1.034GlyCys: 1.034 ± 0.028	3.975GlyAsp: 3.975 ± 0.097	4.856GlyGlu: 4.856 ± 0.08	3.174GlyPhe: 3.174 ± 0.052	5.646GlyGly: 5.646 ± 0.085	1.616GlyHis: 1.616 ± 0.038	4.698GlyIle: 4.698 ± 0.069	3.875GlyLys: 3.875 ± 0.081	7.587GlyLeu: 7.587 ± 0.102	2.293GlyMet: 2.293 ± 0.045	3.151GlyAsn: 3.151 ± 0.141	2.134GlyPro: 2.134 ± 0.037	3.099GlyGln: 3.099 ± 0.064	3.972GlyArg: 3.972 ± 0.062	4.612GlySer: 4.612 ± 0.155	3.93GlyThr: 3.93 ± 0.12	5.96GlyVal: 5.96 ± 0.084	1.375GlyTrp: 1.375 ± 0.036	2.592GlyTyr: 2.592 ± 0.051	0.0GlyXaa: 0.0 ± 0.0
His	2.002HisAla: 2.002 ± 0.038	0.311HisCys: 0.311 ± 0.016	1.192HisAsp: 1.192 ± 0.03	1.121HisGlu: 1.121 ± 0.031	1.025HisPhe: 1.025 ± 0.031	1.806HisGly: 1.806 ± 0.038	0.702HisHis: 0.702 ± 0.025	1.28HisIle: 1.28 ± 0.034	0.7HisLys: 0.7 ± 0.026	2.151HisLeu: 2.151 ± 0.043	0.515HisMet: 0.515 ± 0.021	0.867HisAsn: 0.867 ± 0.03	1.271HisPro: 1.271 ± 0.035	1.027HisGln: 1.027 ± 0.029	1.301HisArg: 1.301 ± 0.037	1.34HisSer: 1.34 ± 0.037	1.068HisThr: 1.068 ± 0.031	1.258HisVal: 1.258 ± 0.029	0.413HisTrp: 0.413 ± 0.018	0.837HisTyr: 0.837 ± 0.021	0.0HisXaa: 0.0 ± 0.0
Ile	6.132IleAla: 6.132 ± 0.08	0.583IleCys: 0.583 ± 0.024	3.494IleAsp: 3.494 ± 0.072	3.151IleGlu: 3.151 ± 0.066	1.935IlePhe: 1.935 ± 0.047	4.346IleGly: 4.346 ± 0.078	1.109IleHis: 1.109 ± 0.031	3.137IleIle: 3.137 ± 0.05	2.06IleLys: 2.06 ± 0.047	4.462IleLeu: 4.462 ± 0.062	1.164IleMet: 1.164 ± 0.038	2.602IleAsn: 2.602 ± 0.054	2.39IlePro: 2.39 ± 0.044	1.652IleGln: 1.652 ± 0.036	2.814IleArg: 2.814 ± 0.048	3.74IleSer: 3.74 ± 0.061	3.512IleThr: 3.512 ± 0.07	3.822IleVal: 3.822 ± 0.059	0.651IleTrp: 0.651 ± 0.023	1.524IleTyr: 1.524 ± 0.045	0.0IleXaa: 0.0 ± 0.0
Lys	4.012LysAla: 4.012 ± 0.084	0.262LysCys: 0.262 ± 0.015	1.998LysAsp: 1.998 ± 0.05	2.131LysGlu: 2.131 ± 0.047	1.039LysPhe: 1.039 ± 0.029	2.766LysGly: 2.766 ± 0.054	0.802LysHis: 0.802 ± 0.024	2.057LysIle: 2.057 ± 0.048	1.988LysLys: 1.988 ± 0.053	3.883LysLeu: 3.883 ± 0.06	1.129LysMet: 1.129 ± 0.03	1.563LysAsn: 1.563 ± 0.041	1.944LysPro: 1.944 ± 0.042	1.715LysGln: 1.715 ± 0.041	2.354LysArg: 2.354 ± 0.048	2.192LysSer: 2.192 ± 0.043	2.409LysThr: 2.409 ± 0.046	2.807LysVal: 2.807 ± 0.051	0.449LysTrp: 0.449 ± 0.027	1.046LysTyr: 1.046 ± 0.03	0.0LysXaa: 0.0 ± 0.0
Leu	11.913LeuAla: 11.913 ± 0.132	1.297LeuCys: 1.297 ± 0.041	5.507LeuAsp: 5.507 ± 0.073	5.643LeuGlu: 5.643 ± 0.097	4.065LeuPhe: 4.065 ± 0.07	7.464LeuGly: 7.464 ± 0.109	2.299LeuHis: 2.299 ± 0.046	5.414LeuIle: 5.414 ± 0.073	4.221LeuLys: 4.221 ± 0.064	11.954LeuLeu: 11.954 ± 0.186	2.946LeuMet: 2.946 ± 0.062	4.321LeuAsn: 4.321 ± 0.067	5.578LeuPro: 5.578 ± 0.084	3.896LeuGln: 3.896 ± 0.066	6.468LeuArg: 6.468 ± 0.112	7.422LeuSer: 7.422 ± 0.079	6.47LeuThr: 6.47 ± 0.097	7.234LeuVal: 7.234 ± 0.092	1.527LeuTrp: 1.527 ± 0.038	2.523LeuTyr: 2.523 ± 0.047	0.0LeuXaa: 0.0 ± 0.0
Met	2.861MetAla: 2.861 ± 0.052	0.195MetCys: 0.195 ± 0.011	1.179MetAsp: 1.179 ± 0.029	1.251MetGlu: 1.251 ± 0.03	0.824MetPhe: 0.824 ± 0.028	1.8MetGly: 1.8 ± 0.041	0.47MetHis: 0.47 ± 0.021	1.26MetIle: 1.26 ± 0.035	1.311MetLys: 1.311 ± 0.032	3.08MetLeu: 3.08 ± 0.054	0.865MetMet: 0.865 ± 0.03	1.031MetAsn: 1.031 ± 0.028	1.384MetPro: 1.384 ± 0.038	1.208MetGln: 1.208 ± 0.031	1.478MetArg: 1.478 ± 0.037	1.868MetSer: 1.868 ± 0.048	1.723MetThr: 1.723 ± 0.036	1.949MetVal: 1.949 ± 0.046	0.253MetTrp: 0.253 ± 0.013	0.489MetTyr: 0.489 ± 0.019	0.0MetXaa: 0.0 ± 0.0
Asn	3.849AsnAla: 3.849 ± 0.073	0.339AsnCys: 0.339 ± 0.017	2.047AsnAsp: 2.047 ± 0.041	1.919AsnGlu: 1.919 ± 0.044	1.284AsnPhe: 1.284 ± 0.031	3.261AsnGly: 3.261 ± 0.118	0.846AsnHis: 0.846 ± 0.026	2.273AsnIle: 2.273 ± 0.056	1.419AsnLys: 1.419 ± 0.04	3.468AsnLeu: 3.468 ± 0.058	0.869AsnMet: 0.869 ± 0.029	1.709AsnAsn: 1.709 ± 0.052	2.035AsnPro: 2.035 ± 0.042	1.509AsnGln: 1.509 ± 0.044	2.072AsnArg: 2.072 ± 0.044	2.165AsnSer: 2.165 ± 0.06	2.169AsnThr: 2.169 ± 0.06	2.676AsnVal: 2.676 ± 0.064	0.587AsnTrp: 0.587 ± 0.033	1.171AsnTyr: 1.171 ± 0.032	0.0AsnXaa: 0.0 ± 0.0
Pro	4.496ProAla: 4.496 ± 0.06	0.38ProCys: 0.38 ± 0.019	2.876ProAsp: 2.876 ± 0.058	3.225ProGlu: 3.225 ± 0.054	1.734ProPhe: 1.734 ± 0.04	3.766ProGly: 3.766 ± 0.061	0.99ProHis: 0.99 ± 0.027	1.608ProIle: 1.608 ± 0.047	1.324ProLys: 1.324 ± 0.036	5.166ProLeu: 5.166 ± 0.068	1.043ProMet: 1.043 ± 0.033	1.231ProAsn: 1.231 ± 0.034	1.744ProPro: 1.744 ± 0.044	2.602ProGln: 2.602 ± 0.051	2.017ProArg: 2.017 ± 0.045	2.167ProSer: 2.167 ± 0.053	2.327ProThr: 2.327 ± 0.095	4.124ProVal: 4.124 ± 0.06	0.771ProTrp: 0.771 ± 0.026	1.115ProTyr: 1.115 ± 0.03	0.0ProXaa: 0.0 ± 0.0
Gln	4.991GlnAla: 4.991 ± 0.085	0.386GlnCys: 0.386 ± 0.021	1.891GlnAsp: 1.891 ± 0.044	2.21GlnGlu: 2.21 ± 0.045	1.365GlnPhe: 1.365 ± 0.035	3.119GlnGly: 3.119 ± 0.056	1.182GlnHis: 1.182 ± 0.038	2.157GlnIle: 2.157 ± 0.042	1.723GlnLys: 1.723 ± 0.043	4.825GlnLeu: 4.825 ± 0.088	1.216GlnMet: 1.216 ± 0.035	1.52GlnAsn: 1.52 ± 0.04	2.254GlnPro: 2.254 ± 0.055	3.173GlnGln: 3.173 ± 0.08	3.246GlnArg: 3.246 ± 0.069	2.332GlnSer: 2.332 ± 0.042	2.341GlnThr: 2.341 ± 0.055	3.086GlnVal: 3.086 ± 0.056	0.651GlnTrp: 0.651 ± 0.024	1.155GlnTyr: 1.155 ± 0.031	0.0GlnXaa: 0.0 ± 0.0
Arg	5.131ArgAla: 5.131 ± 0.084	0.635ArgCys: 0.635 ± 0.028	3.215ArgAsp: 3.215 ± 0.059	4.021ArgGlu: 4.021 ± 0.075	2.607ArgPhe: 2.607 ± 0.049	3.58ArgGly: 3.58 ± 0.06	1.616ArgHis: 1.616 ± 0.045	3.317ArgIle: 3.317 ± 0.058	2.125ArgLys: 2.125 ± 0.044	6.922ArgLeu: 6.922 ± 0.116	1.611ArgMet: 1.611 ± 0.04	2.105ArgAsn: 2.105 ± 0.045	2.311ArgPro: 2.311 ± 0.044	3.076ArgGln: 3.076 ± 0.06	3.8ArgArg: 3.8 ± 0.082	3.007ArgSer: 3.007 ± 0.058	2.654ArgThr: 2.654 ± 0.041	3.971ArgVal: 3.971 ± 0.068	1.08ArgTrp: 1.08 ± 0.03	2.198ArgTyr: 2.198 ± 0.049	0.0ArgXaa: 0.0 ± 0.0
Ser	6.273SerAla: 6.273 ± 0.086	0.559SerCys: 0.559 ± 0.023	3.18SerAsp: 3.18 ± 0.05	3.264SerGlu: 3.264 ± 0.047	2.276SerPhe: 2.276 ± 0.045	5.811SerGly: 5.811 ± 0.106	1.468SerHis: 1.468 ± 0.036	2.852SerIle: 2.852 ± 0.057	1.91SerLys: 1.91 ± 0.042	6.692SerLeu: 6.692 ± 0.089	1.444SerMet: 1.444 ± 0.034	1.928SerAsn: 1.928 ± 0.049	2.644SerPro: 2.644 ± 0.05	2.583SerGln: 2.583 ± 0.053	3.7SerArg: 3.7 ± 0.06	3.654SerSer: 3.654 ± 0.066	3.23SerThr: 3.23 ± 0.103	4.54SerVal: 4.54 ± 0.08	1.059SerTrp: 1.059 ± 0.037	1.622SerTyr: 1.622 ± 0.04	0.0SerXaa: 0.0 ± 0.0
Thr	5.204ThrAla: 5.204 ± 0.099	0.503ThrCys: 0.503 ± 0.02	2.846ThrAsp: 2.846 ± 0.144	2.555ThrGlu: 2.555 ± 0.069	2.119ThrPhe: 2.119 ± 0.047	4.713ThrGly: 4.713 ± 0.089	1.231ThrHis: 1.231 ± 0.033	2.861ThrIle: 2.861 ± 0.104	1.375ThrLys: 1.375 ± 0.04	7.765ThrLeu: 7.765 ± 0.1	1.081ThrMet: 1.081 ± 0.026	1.586ThrAsn: 1.586 ± 0.048	3.515ThrPro: 3.515 ± 0.114	2.341ThrGln: 2.341 ± 0.046	3.307ThrArg: 3.307 ± 0.058	3.011ThrSer: 3.011 ± 0.058	3.141ThrThr: 3.141 ± 0.151	4.266ThrVal: 4.266 ± 0.164	0.873ThrTrp: 0.873 ± 0.032	1.084ThrTyr: 1.084 ± 0.036	0.0ThrXaa: 0.0 ± 0.0
Val	7.337ValAla: 7.337 ± 0.089	0.76ValCys: 0.76 ± 0.021	3.854ValAsp: 3.854 ± 0.086	4.084ValGlu: 4.084 ± 0.074	2.529ValPhe: 2.529 ± 0.052	4.988ValGly: 4.988 ± 0.104	1.278ValHis: 1.278 ± 0.034	4.225ValIle: 4.225 ± 0.059	2.889ValLys: 2.889 ± 0.052	7.61ValLeu: 7.61 ± 0.103	2.157ValMet: 2.157 ± 0.043	2.867ValAsn: 2.867 ± 0.052	3.051ValPro: 3.051 ± 0.051	2.607ValGln: 2.607 ± 0.051	3.989ValArg: 3.989 ± 0.065	4.712ValSer: 4.712 ± 0.068	4.562ValThr: 4.562 ± 0.141	5.644ValVal: 5.644 ± 0.08	0.993ValTrp: 0.993 ± 0.025	1.655ValTyr: 1.655 ± 0.039	0.0ValXaa: 0.0 ± 0.0
Trp	1.035TrpAla: 1.035 ± 0.031	0.206TrpCys: 0.206 ± 0.012	0.665TrpAsp: 0.665 ± 0.024	0.568TrpGlu: 0.568 ± 0.02	0.684TrpPhe: 0.684 ± 0.024	0.964TrpGly: 0.964 ± 0.031	0.46TrpHis: 0.46 ± 0.021	0.692TrpIle: 0.692 ± 0.024	0.498TrpLys: 0.498 ± 0.018	2.429TrpLeu: 2.429 ± 0.061	0.424TrpMet: 0.424 ± 0.019	0.498TrpAsn: 0.498 ± 0.02	0.693TrpPro: 0.693 ± 0.028	1.256TrpGln: 1.256 ± 0.036	1.268TrpArg: 1.268 ± 0.036	0.912TrpSer: 0.912 ± 0.051	0.633TrpThr: 0.633 ± 0.037	0.938TrpVal: 0.938 ± 0.027	0.237TrpTrp: 0.237 ± 0.013	0.433TrpTyr: 0.433 ± 0.019	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.415TyrAla: 2.415 ± 0.044	0.33TyrCys: 0.33 ± 0.015	1.59TyrAsp: 1.59 ± 0.047	1.189TyrGlu: 1.189 ± 0.033	1.108TyrPhe: 1.108 ± 0.033	2.197TyrGly: 2.197 ± 0.043	0.645TyrHis: 0.645 ± 0.021	1.35TyrIle: 1.35 ± 0.037	0.845TyrLys: 0.845 ± 0.027	2.861TyrLeu: 2.861 ± 0.053	0.574TyrMet: 0.574 ± 0.02	1.024TyrAsn: 1.024 ± 0.032	1.322TyrPro: 1.322 ± 0.033	1.437TyrGln: 1.437 ± 0.036	1.902TyrArg: 1.902 ± 0.043	1.782TyrSer: 1.782 ± 0.047	1.451TyrThr: 1.451 ± 0.048	1.645TyrVal: 1.645 ± 0.039	0.417TyrTrp: 0.417 ± 0.019	0.88TyrTyr: 0.88 ± 0.031	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4055 proteins (1319694 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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