Fusobacterium nucleatum subsp. animalis ATCC 51191
Average proteome isoelectric point is 6.85
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2777 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|F9ER90|F9ER90_FUSNU AAA_15 domain-containing protein (Fragment) OS=Fusobacterium nucleatum subsp. animalis ATCC 51191 OX=997347 GN=HMPREF9094_2445 PE=4 SV=1
MM1 pKa = 7.3 TNINEE6 pKa = 4.24 DD7 pKa = 3.2 HH8 pKa = 7.2 SEE10 pKa = 3.98 LLNNIYY16 pKa = 10.9 NKK18 pKa = 10.43 DD19 pKa = 3.16 SEE21 pKa = 5.09 YY22 pKa = 11.23 YY23 pKa = 9.08 FTEE26 pKa = 3.92 KK27 pKa = 10.59 DD28 pKa = 3.53 FKK30 pKa = 10.81 IANQFLNNYY39 pKa = 7.77 DD40 pKa = 3.71 LKK42 pKa = 10.69 IYY44 pKa = 10.87 NSTEE48 pKa = 3.81 EE49 pKa = 3.92 DD50 pKa = 3.64 TMISEE55 pKa = 4.6 VPNFYY60 pKa = 11.16 YY61 pKa = 10.43 DD62 pKa = 2.95 IFKK65 pKa = 10.88 DD66 pKa = 3.99 YY67 pKa = 10.62 VTDD70 pKa = 4.17 DD71 pKa = 3.31 YY72 pKa = 11.78 RR73 pKa = 11.84 EE74 pKa = 4.03 YY75 pKa = 11.58 LEE77 pKa = 4.04 ITSKK81 pKa = 10.82 EE82 pKa = 4.03 KK83 pKa = 10.84 EE84 pKa = 4.06 EE85 pKa = 4.35 PYY87 pKa = 9.39 YY88 pKa = 10.7 TLDD91 pKa = 3.52 GRR93 pKa = 11.84 DD94 pKa = 3.36 VLVSYY99 pKa = 10.78 DD100 pKa = 3.81 KK101 pKa = 11.09 IADD104 pKa = 4.23 RR105 pKa = 11.84 LLTWEE110 pKa = 4.53 NFLKK114 pKa = 10.98 NILIVII120 pKa = 4.66
Molecular weight: 14.61 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.919
IPC2_protein 4.202
IPC_protein 4.139
Toseland 3.961
ProMoST 4.279
Dawson 4.101
Bjellqvist 4.266
Wikipedia 3.999
Rodwell 3.973
Grimsley 3.872
Solomon 4.101
Lehninger 4.05
Nozaki 4.215
DTASelect 4.406
Thurlkill 3.986
EMBOSS 4.024
Sillero 4.253
Patrickios 1.952
IPC_peptide 4.101
IPC2_peptide 4.24
IPC2.peptide.svr19 4.139
Protein with the highest isoelectric point:
>tr|F9EQJ3|F9EQJ3_FUSNU Uncharacterized protein OS=Fusobacterium nucleatum subsp. animalis ATCC 51191 OX=997347 GN=HMPREF9094_2198 PE=4 SV=1
MM1 pKa = 7.41 QLQLKK6 pKa = 9.19 IKK8 pKa = 8.63 KK9 pKa = 6.51 TRR11 pKa = 11.84 KK12 pKa = 8.12 IKK14 pKa = 10.12 RR15 pKa = 11.84 VKK17 pKa = 9.95 IKK19 pKa = 10.63 NLIRR23 pKa = 11.84 KK24 pKa = 8.89 RR25 pKa = 11.84 RR26 pKa = 11.84 IKK28 pKa = 10.07 KK29 pKa = 9.56 VRR31 pKa = 11.84 IIKK34 pKa = 9.95 KK35 pKa = 10.17 KK36 pKa = 9.89 KK37 pKa = 9.11 LKK39 pKa = 9.91 KK40 pKa = 10.52 LKK42 pKa = 10.38 ILL44 pKa = 3.85
Molecular weight: 5.52 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.443
IPC2_protein 11.067
IPC_protein 12.544
Toseland 12.749
ProMoST 13.203
Dawson 12.749
Bjellqvist 12.72
Wikipedia 13.203
Rodwell 12.837
Grimsley 12.793
Solomon 13.217
Lehninger 13.115
Nozaki 12.735
DTASelect 12.72
Thurlkill 12.735
EMBOSS 13.232
Sillero 12.735
Patrickios 12.559
IPC_peptide 13.217
IPC2_peptide 12.193
IPC2.peptide.svr19 8.96
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2777
0
2777
661607
37
3707
238.2
26.97
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.632 ± 0.059
0.783 ± 0.015
5.439 ± 0.042
7.723 ± 0.07
4.672 ± 0.042
6.6 ± 0.072
1.196 ± 0.02
9.751 ± 0.059
9.967 ± 0.071
9.086 ± 0.059
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.469 ± 0.027
6.439 ± 0.067
2.666 ± 0.03
2.199 ± 0.022
3.252 ± 0.034
5.972 ± 0.053
5.011 ± 0.052
6.17 ± 0.047
0.661 ± 0.014
4.31 ± 0.042
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here