Enterobacteria phage mEp043 c-1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Aguilavirus; Enterobacteria virus mEp043

Average proteome isoelectric point is 6.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 69 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K7PK00|K7PK00_9CAUD Tail assembly protein OS=Enterobacteria phage mEp043 c-1 OX=1147149 GN=mEp043_018 PE=4 SV=1
MM1 pKa = 7.4LAGMTSSEE9 pKa = 4.2LGDD12 pKa = 2.82WHH14 pKa = 6.96LFYY17 pKa = 10.65RR18 pKa = 11.84EE19 pKa = 5.17HH20 pKa = 6.67YY21 pKa = 9.92FQDD24 pKa = 3.55AQLDD28 pKa = 3.73AHH30 pKa = 6.77FSGLLYY36 pKa = 10.72SISTLFFRR44 pKa = 11.84DD45 pKa = 3.71PEE47 pKa = 4.13LTPAHH52 pKa = 6.63FSLLSPSEE60 pKa = 4.19SVISDD65 pKa = 4.45DD66 pKa = 5.53EE67 pKa = 5.64PDD69 pKa = 5.1DD70 pKa = 4.14DD71 pKa = 4.64TLMTAAEE78 pKa = 5.28GITGGIRR85 pKa = 11.84YY86 pKa = 9.1GPADD90 pKa = 3.26

Molecular weight: 9.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K7PHQ8|K7PHQ8_9CAUD Uncharacterized protein OS=Enterobacteria phage mEp043 c-1 OX=1147149 GN=mEp043_053 PE=4 SV=1
MM1 pKa = 8.07DD2 pKa = 4.41AQTRR6 pKa = 11.84RR7 pKa = 11.84RR8 pKa = 11.84EE9 pKa = 3.77RR10 pKa = 11.84RR11 pKa = 11.84AEE13 pKa = 4.15KK14 pKa = 9.26QAQWKK19 pKa = 8.58AANPLLVGVSAKK31 pKa = 9.96PVNRR35 pKa = 11.84PILSLNRR42 pKa = 11.84KK43 pKa = 7.08PKK45 pKa = 10.54SRR47 pKa = 11.84VEE49 pKa = 4.08SALNPIDD56 pKa = 3.85LTVLAEE62 pKa = 3.84YY63 pKa = 10.59HH64 pKa = 5.65EE65 pKa = 4.71QIEE68 pKa = 4.84SNLQRR73 pKa = 11.84IEE75 pKa = 4.31RR76 pKa = 11.84KK77 pKa = 7.93NQRR80 pKa = 11.84TWYY83 pKa = 8.98SKK85 pKa = 9.72PRR87 pKa = 11.84SEE89 pKa = 4.9MGVTCVGRR97 pKa = 11.84QKK99 pKa = 10.52IKK101 pKa = 10.76LGSKK105 pKa = 9.94PLII108 pKa = 4.11

Molecular weight: 12.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

69

0

69

13156

43

1226

190.7

21.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.912 ± 0.492

1.064 ± 0.147

5.853 ± 0.391

6.605 ± 0.534

3.154 ± 0.148

7.335 ± 0.394

1.543 ± 0.184

5.579 ± 0.258

6.172 ± 0.332

7.692 ± 0.301

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.767 ± 0.193

4.219 ± 0.263

3.785 ± 0.293

4.834 ± 0.536

6.05 ± 0.437

6.712 ± 0.304

5.822 ± 0.347

6.134 ± 0.33

1.839 ± 0.184

2.926 ± 0.178
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski