Cephaloticoccus capnophilus
Average proteome isoelectric point is 6.74
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1549 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A139SNN4|A0A139SNN4_9BACT TonB_dep_Rec domain-containing protein OS=Cephaloticoccus capnophilus OX=1548208 GN=AXK12_03815 PE=3 SV=1
MM1 pKa = 7.16 GAEE4 pKa = 4.59 FAAVPGTEE12 pKa = 4.02 LKK14 pKa = 10.56 ALRR17 pKa = 11.84 VSGQTVPDD25 pKa = 3.56 APYY28 pKa = 10.85 SFSGLQNGLNTLLLEE43 pKa = 4.1 VDD45 pKa = 4.22 YY46 pKa = 11.42 GPEE49 pKa = 4.15 PGSNNQSSGTVTGNLRR65 pKa = 11.84 IYY67 pKa = 6.95 EE68 pKa = 4.24 QPYY71 pKa = 9.99 SMVIGTVYY79 pKa = 9.63 TQDD82 pKa = 3.39 SGTLMDD88 pKa = 5.81 ADD90 pKa = 4.36 DD91 pKa = 5.16 LNVHH95 pKa = 5.74 EE96 pKa = 4.77 VEE98 pKa = 4.47 DD99 pKa = 3.94 VRR101 pKa = 11.84 GLEE104 pKa = 4.13 TTSAVIDD111 pKa = 4.11 ALTITATT118 pKa = 3.45
Molecular weight: 12.44 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.76
IPC2_protein 3.859
IPC_protein 3.783
Toseland 3.592
ProMoST 3.948
Dawson 3.77
Bjellqvist 3.923
Wikipedia 3.694
Rodwell 3.617
Grimsley 3.503
Solomon 3.745
Lehninger 3.706
Nozaki 3.897
DTASelect 4.075
Thurlkill 3.656
EMBOSS 3.706
Sillero 3.897
Patrickios 1.875
IPC_peptide 3.745
IPC2_peptide 3.872
IPC2.peptide.svr19 3.806
Protein with the highest isoelectric point:
>tr|A0A139SMC8|A0A139SMC8_9BACT 4-hydroxythreonine-4-phosphate dehydrogenase OS=Cephaloticoccus capnophilus OX=1548208 GN=AXK12_04695 PE=4 SV=1
MM1 pKa = 7.15 QPTFRR6 pKa = 11.84 PHH8 pKa = 5.63 RR9 pKa = 11.84 KK10 pKa = 8.98 KK11 pKa = 10.38 RR12 pKa = 11.84 ARR14 pKa = 11.84 QIGFRR19 pKa = 11.84 ARR21 pKa = 11.84 KK22 pKa = 7.8 ATASGRR28 pKa = 11.84 KK29 pKa = 8.86 VLAARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.76 GRR39 pKa = 11.84 KK40 pKa = 8.94 RR41 pKa = 11.84 LTVVV45 pKa = 3.08
Molecular weight: 5.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.257
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.705
Grimsley 13.042
Solomon 13.51
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.427
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.173
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1549
0
1549
557160
41
1951
359.7
39.27
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.042 ± 0.083
0.928 ± 0.02
4.972 ± 0.042
6.113 ± 0.054
3.882 ± 0.038
8.045 ± 0.068
2.262 ± 0.032
5.013 ± 0.043
3.536 ± 0.046
10.89 ± 0.071
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.765 ± 0.029
2.821 ± 0.042
5.238 ± 0.045
3.448 ± 0.035
7.079 ± 0.055
5.968 ± 0.046
5.489 ± 0.044
6.618 ± 0.049
1.323 ± 0.028
2.57 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here