Equus caballus papillomavirus 5
Average proteome isoelectric point is 6.69
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|K9M8U7|K9M8U7_9PAPI E4 OS=Equus caballus papillomavirus 5 OX=1235429 PE=4 SV=1
MM1 pKa = 8.06 DD2 pKa = 5.48 APPVKK7 pKa = 9.69 RR8 pKa = 11.84 RR9 pKa = 11.84 KK10 pKa = 9.37 RR11 pKa = 11.84 AAVDD15 pKa = 3.33 QLYY18 pKa = 7.79 KK19 pKa = 7.73 TCKK22 pKa = 9.48 MGGDD26 pKa = 4.21 CPPDD30 pKa = 3.34 VVPKK34 pKa = 10.91 VEE36 pKa = 4.28 GDD38 pKa = 3.71 TVADD42 pKa = 6.04 RR43 pKa = 11.84 ILKK46 pKa = 8.42 WLSSFFYY53 pKa = 10.43 FGNLGISTGRR63 pKa = 11.84 GTGGRR68 pKa = 11.84 LGYY71 pKa = 8.94 TPLGGGGGHH80 pKa = 6.55 AAEE83 pKa = 4.93 GGGVRR88 pKa = 11.84 VGAPISTLGVGVDD101 pKa = 3.56 AVGPAEE107 pKa = 4.25 AVPVDD112 pKa = 3.9 VLQPSAPSIIPLGEE126 pKa = 4.12 GVSVDD131 pKa = 3.64 TVDD134 pKa = 4.68 VIAEE138 pKa = 4.07 VLPPPGGARR147 pKa = 11.84 PEE149 pKa = 4.11 VTAEE153 pKa = 4.16 VPGQPATIDD162 pKa = 3.26 VAMDD166 pKa = 3.07 VVPRR170 pKa = 11.84 LRR172 pKa = 11.84 AAVSRR177 pKa = 11.84 STFHH181 pKa = 6.67 NPAFQVEE188 pKa = 4.43 LQSLGTGEE196 pKa = 4.44 SSASEE201 pKa = 3.65 QAFVFGHH208 pKa = 5.8 AGGRR212 pKa = 11.84 LVDD215 pKa = 3.89 TVGEE219 pKa = 4.49 DD220 pKa = 3.21 IEE222 pKa = 4.65 MVALGDD228 pKa = 3.84 GVPRR232 pKa = 11.84 TSTPRR237 pKa = 11.84 SSSATPRR244 pKa = 11.84 SRR246 pKa = 11.84 LWGRR250 pKa = 11.84 RR251 pKa = 11.84 FEE253 pKa = 4.26 QVQVADD259 pKa = 4.18 PAFLSAPDD267 pKa = 3.71 TLVQYY272 pKa = 10.23 GFSNPAYY279 pKa = 9.95 DD280 pKa = 4.17 PEE282 pKa = 6.07 ASLVFPYY289 pKa = 10.61 TEE291 pKa = 4.32 GEE293 pKa = 3.81 ARR295 pKa = 11.84 AAPSTLFQDD304 pKa = 3.47 VAYY307 pKa = 10.42 LGRR310 pKa = 11.84 PEE312 pKa = 3.97 ITSHH316 pKa = 6.45 KK317 pKa = 9.85 EE318 pKa = 3.71 SIGVSRR324 pKa = 11.84 LGRR327 pKa = 11.84 RR328 pKa = 11.84 ATVQTRR334 pKa = 11.84 SGTIIGAEE342 pKa = 3.65 VHH344 pKa = 6.25 FRR346 pKa = 11.84 YY347 pKa = 9.68 EE348 pKa = 4.04 LSPITSEE355 pKa = 5.02 DD356 pKa = 3.23 IEE358 pKa = 4.92 LSVLTTQDD366 pKa = 3.22 EE367 pKa = 4.51 EE368 pKa = 4.36 ALSVVDD374 pKa = 5.88 LEE376 pKa = 4.96 SLTSAYY382 pKa = 9.65 SEE384 pKa = 4.42 RR385 pKa = 11.84 EE386 pKa = 4.06 LLEE389 pKa = 5.27 DD390 pKa = 4.29 DD391 pKa = 3.84 QPNIHH396 pKa = 6.36 GVLSFTDD403 pKa = 3.62 EE404 pKa = 4.2 EE405 pKa = 5.08 GATTSLPLAPPQARR419 pKa = 11.84 FFYY422 pKa = 10.15 TGPFLQGGRR431 pKa = 11.84 GVSDD435 pKa = 3.42 GRR437 pKa = 11.84 GQAAGEE443 pKa = 4.24 SSHH446 pKa = 7.22 PGTDD450 pKa = 3.45 VIIEE454 pKa = 4.05 YY455 pKa = 10.06 PEE457 pKa = 5.0 AGGSYY462 pKa = 9.76 FLHH465 pKa = 6.37 PTAPCRR471 pKa = 11.84 RR472 pKa = 11.84 KK473 pKa = 9.94 RR474 pKa = 11.84 RR475 pKa = 11.84 YY476 pKa = 9.9 CFADD480 pKa = 3.62 GLLDD484 pKa = 4.31 AGQSEE489 pKa = 5.1 VLSPPCACYY498 pKa = 10.36
Molecular weight: 52.51 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.766
IPC2_protein 4.774
IPC_protein 4.724
Toseland 4.584
ProMoST 4.825
Dawson 4.673
Bjellqvist 4.813
Wikipedia 4.533
Rodwell 4.571
Grimsley 4.495
Solomon 4.673
Lehninger 4.622
Nozaki 4.774
DTASelect 4.94
Thurlkill 4.584
EMBOSS 4.558
Sillero 4.851
Patrickios 3.923
IPC_peptide 4.673
IPC2_peptide 4.838
IPC2.peptide.svr19 4.816
Protein with the highest isoelectric point:
>tr|K9M934|K9M934_9PAPI E7 OS=Equus caballus papillomavirus 5 OX=1235429 PE=4 SV=1
MM1 pKa = 7.36 KK2 pKa = 9.89 PSPPLLSPAQVVVTARR18 pKa = 11.84 KK19 pKa = 9.54 EE20 pKa = 3.92 EE21 pKa = 4.25 AGTDD25 pKa = 3.67 QTDD28 pKa = 3.49 SAATQAQGGLAQFPEE43 pKa = 4.33 TLVDD47 pKa = 3.58 YY48 pKa = 10.46 LKK50 pKa = 10.86 RR51 pKa = 11.84 NHH53 pKa = 6.25 LTGADD58 pKa = 3.21 VWPRR62 pKa = 11.84 QSGCLLNSRR71 pKa = 11.84 GKK73 pKa = 10.55 ALLQCMTAGTAEE85 pKa = 4.18 EE86 pKa = 4.63 APLAVTPSLASEE98 pKa = 3.78 ARR100 pKa = 11.84 GYY102 pKa = 9.87 WEE104 pKa = 5.05 IPPPPAPCRR113 pKa = 11.84 ARR115 pKa = 11.84 MRR117 pKa = 11.84 RR118 pKa = 11.84 TRR120 pKa = 11.84 LAHH123 pKa = 5.66 RR124 pKa = 11.84 QRR126 pKa = 11.84 LKK128 pKa = 8.98 QTLWHH133 pKa = 6.3 LQRR136 pKa = 11.84 EE137 pKa = 4.62 LSEE140 pKa = 3.8 LMYY143 pKa = 10.74 WMDD146 pKa = 3.92 VGILL150 pKa = 3.51
Molecular weight: 16.63 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.623
IPC2_protein 8.361
IPC_protein 8.492
Toseland 8.785
ProMoST 8.814
Dawson 9.121
Bjellqvist 9.209
Wikipedia 9.18
Rodwell 9.18
Grimsley 8.697
Solomon 9.385
Lehninger 9.37
Nozaki 9.414
DTASelect 9.033
Thurlkill 9.107
EMBOSS 9.311
Sillero 9.355
Patrickios 5.016
IPC_peptide 9.37
IPC2_peptide 8.273
IPC2.peptide.svr19 7.828
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
2411
84
632
344.4
38.24
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.715 ± 0.88
2.406 ± 0.57
5.89 ± 0.42
5.931 ± 0.216
4.106 ± 0.57
8.295 ± 1.13
1.742 ± 0.191
2.986 ± 0.541
4.148 ± 0.608
8.337 ± 0.45
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.701 ± 0.327
3.36 ± 0.892
6.802 ± 0.528
4.148 ± 0.26
7.258 ± 0.414
7.507 ± 0.604
5.641 ± 0.398
6.802 ± 0.539
1.866 ± 0.395
3.36 ± 0.432
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here