Donghicola tyrosinivorans
Average proteome isoelectric point is 6.07
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4472 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2T0W7T4|A0A2T0W7T4_9RHOB Uncharacterized protein OS=Donghicola tyrosinivorans OX=1652492 GN=CLV74_1391 PE=4 SV=1
MM1 pKa = 7.14 ATSTTFQVMYY11 pKa = 10.02 LGQRR15 pKa = 11.84 ALIDD19 pKa = 3.42 TTQGNNTAEE28 pKa = 4.12 NAAGILDD35 pKa = 4.34 TYY37 pKa = 11.34 GSASDD42 pKa = 4.76 PMYY45 pKa = 10.83 NWVGTLYY52 pKa = 10.99 ADD54 pKa = 4.74 YY55 pKa = 11.14 LLEE58 pKa = 5.5 DD59 pKa = 5.18 DD60 pKa = 4.68 NNSYY64 pKa = 11.11 DD65 pKa = 3.35 IDD67 pKa = 3.93 NGGGRR72 pKa = 11.84 DD73 pKa = 3.26 RR74 pKa = 11.84 FHH76 pKa = 8.1 VNGFGTQEE84 pKa = 3.43 FDD86 pKa = 3.89 AVAEE90 pKa = 4.07 YY91 pKa = 10.35 AITLTYY97 pKa = 10.88 VDD99 pKa = 4.32 GTTAQITAYY108 pKa = 10.27 VFQDD112 pKa = 3.76 FQGHH116 pKa = 5.28 TFLAPEE122 pKa = 4.17 TTLNADD128 pKa = 3.49 QAALTAKK135 pKa = 9.94 PIQSLALTAVTKK147 pKa = 10.84 SSGDD151 pKa = 3.39 TTAGDD156 pKa = 4.08 MIAARR161 pKa = 11.84 QTGTYY166 pKa = 7.21 KK167 pKa = 10.49 TPVDD171 pKa = 3.64 GTTGNDD177 pKa = 3.29 SMGVGYY183 pKa = 9.23 TDD185 pKa = 3.5 AQGDD189 pKa = 4.06 QITAGNDD196 pKa = 3.3 YY197 pKa = 10.78 ILAGAGNDD205 pKa = 3.37 TVTAGDD211 pKa = 3.77 GNDD214 pKa = 3.57 FVSGGTGADD223 pKa = 4.33 LIYY226 pKa = 10.74 GGNGNDD232 pKa = 3.56 SLQGNDD238 pKa = 4.27 GNDD241 pKa = 3.51 TIYY244 pKa = 11.43 GEE246 pKa = 4.73 LGADD250 pKa = 4.03 TIDD253 pKa = 4.28 GGAGDD258 pKa = 5.3 DD259 pKa = 3.95 SILYY263 pKa = 10.65 GEE265 pKa = 4.89 GADD268 pKa = 3.64 VVYY271 pKa = 10.77 GGAGNDD277 pKa = 3.88 YY278 pKa = 10.79 IDD280 pKa = 5.94 DD281 pKa = 3.84 INGAQLNGANTIYY294 pKa = 10.92 GGDD297 pKa = 4.05 GNDD300 pKa = 4.63 SIYY303 pKa = 10.75 TGNDD307 pKa = 2.63 GDD309 pKa = 5.43 LIYY312 pKa = 10.82 GGQGTDD318 pKa = 3.34 WIDD321 pKa = 3.61 GEE323 pKa = 4.6 LGNDD327 pKa = 3.52 TVYY330 pKa = 11.24 GGADD334 pKa = 3.54 NDD336 pKa = 4.28 TVMGGAGNDD345 pKa = 3.62 SLFGGSGADD354 pKa = 3.94 YY355 pKa = 10.84 IDD357 pKa = 4.31 GGADD361 pKa = 3.06 NDD363 pKa = 4.51 IIYY366 pKa = 10.88 GDD368 pKa = 3.92 FNGNTVNINYY378 pKa = 7.62 TVSSSSLAASTPAARR393 pKa = 11.84 VDD395 pKa = 3.91 DD396 pKa = 4.64 DD397 pKa = 3.82 ATLKK401 pKa = 11.0 VSFDD405 pKa = 3.27 EE406 pKa = 4.51 AATVRR411 pKa = 11.84 LTFGLLEE418 pKa = 4.2 NFEE421 pKa = 4.68 EE422 pKa = 4.61 NIISIDD428 pKa = 3.68 GVVVDD433 pKa = 4.5 IQAMINAGDD442 pKa = 3.74 AVLSGGIAVGATGGVTGTAGSSTSGTITFNVPFTSISVDD481 pKa = 3.42 HH482 pKa = 6.82 TGGGYY487 pKa = 10.56 DD488 pKa = 3.87 DD489 pKa = 3.87 VTVEE493 pKa = 3.95 YY494 pKa = 10.01 LATGGTYY501 pKa = 8.85 TAITDD506 pKa = 3.9 YY507 pKa = 11.82 ANGATGSYY515 pKa = 10.47 AADD518 pKa = 3.38 TVGISGSDD526 pKa = 3.54 TILGGLGDD534 pKa = 3.72 DD535 pKa = 4.73 TIWFGAGDD543 pKa = 3.95 DD544 pKa = 3.89 VVYY547 pKa = 10.83 GGDD550 pKa = 3.9 GNDD553 pKa = 4.49 SIDD556 pKa = 3.81 DD557 pKa = 3.72 RR558 pKa = 11.84 SGDD561 pKa = 3.66 GLVGDD566 pKa = 4.77 NYY568 pKa = 11.17 LDD570 pKa = 4.02 GGAGSDD576 pKa = 4.34 TIWAGYY582 pKa = 10.48 GSDD585 pKa = 3.55 TVLGGDD591 pKa = 4.75 GNDD594 pKa = 4.17 SLHH597 pKa = 6.94 GDD599 pKa = 3.46 QGNDD603 pKa = 3.33 TLSGGLGADD612 pKa = 4.14 TLWGDD617 pKa = 3.83 AGNDD621 pKa = 3.47 VLDD624 pKa = 4.29 GGEE627 pKa = 4.3 GDD629 pKa = 3.83 DD630 pKa = 4.79 HH631 pKa = 7.52 LVAGAGYY638 pKa = 8.02 DD639 pKa = 3.69 TIVASSGIDD648 pKa = 3.1 NVYY651 pKa = 10.68 QFDD654 pKa = 4.99 LNDD657 pKa = 4.1 DD658 pKa = 3.99 NLDD661 pKa = 3.49 GFTNHH666 pKa = 6.53 QIDD669 pKa = 3.87 VSNLTDD675 pKa = 4.24 LEE677 pKa = 4.47 GNPIRR682 pKa = 11.84 WEE684 pKa = 4.08 DD685 pKa = 3.56 VVVTQNVNNHH695 pKa = 5.7 AVLTFPNGEE704 pKa = 4.07 QIILQGITADD714 pKa = 3.58 QVNDD718 pKa = 3.63 KK719 pKa = 11.22 LEE721 pKa = 4.92 LISIGIPCFTVGTMILTPTGEE742 pKa = 4.48 KK743 pKa = 9.41 PIEE746 pKa = 4.01 MLRR749 pKa = 11.84 PGDD752 pKa = 3.63 MVVTRR757 pKa = 11.84 DD758 pKa = 3.88 NGPQPLVWAGSRR770 pKa = 11.84 RR771 pKa = 11.84 LGAGEE776 pKa = 4.29 LARR779 pKa = 11.84 HH780 pKa = 6.19 PEE782 pKa = 3.85 LRR784 pKa = 11.84 PIRR787 pKa = 11.84 IAPGDD792 pKa = 3.42 WAGPRR797 pKa = 11.84 GLLVSPQHH805 pKa = 6.29 GLYY808 pKa = 10.34 AHH810 pKa = 5.68 QSEE813 pKa = 5.16 RR814 pKa = 11.84 GGTDD818 pKa = 2.87 KK819 pKa = 11.26 LIRR822 pKa = 11.84 ATHH825 pKa = 6.41 LARR828 pKa = 11.84 LKK830 pKa = 10.16 GGKK833 pKa = 9.19 VRR835 pKa = 11.84 VANGVTSVTYY845 pKa = 8.77 IHH847 pKa = 6.98 LMFEE851 pKa = 3.88 EE852 pKa = 4.25 HH853 pKa = 6.49 QVIFGNGIASEE864 pKa = 4.21 SFYY867 pKa = 10.34 PGKK870 pKa = 9.93 WGLSSLAAPCRR881 pKa = 11.84 RR882 pKa = 11.84 EE883 pKa = 3.6 ILQLFPEE890 pKa = 4.59 LAGTDD895 pKa = 3.42 VEE897 pKa = 4.32 NAYY900 pKa = 10.12 GQTAMPFARR909 pKa = 11.84 FKK911 pKa = 10.56 EE912 pKa = 4.52 LPDD915 pKa = 3.47 HH916 pKa = 7.08 VYY918 pKa = 10.95 DD919 pKa = 6.05 LSLQTFDD926 pKa = 5.48 RR927 pKa = 11.84 RR928 pKa = 11.84 LLRR931 pKa = 11.84 CGG933 pKa = 3.3
Molecular weight: 96.88 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.669
IPC2_protein 3.681
IPC_protein 3.757
Toseland 3.503
ProMoST 3.935
Dawson 3.77
Bjellqvist 3.91
Wikipedia 3.719
Rodwell 3.567
Grimsley 3.414
Solomon 3.757
Lehninger 3.719
Nozaki 3.872
DTASelect 4.19
Thurlkill 3.567
EMBOSS 3.732
Sillero 3.884
Patrickios 1.1
IPC_peptide 3.757
IPC2_peptide 3.859
IPC2.peptide.svr19 3.769
Protein with the highest isoelectric point:
>tr|A0A2T0WZW2|A0A2T0WZW2_9RHOB Uncharacterized protein OS=Donghicola tyrosinivorans OX=1652492 GN=CLV74_102144 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.44 RR3 pKa = 11.84 TYY5 pKa = 10.31 QPSNLVRR12 pKa = 11.84 KK13 pKa = 9.18 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.42 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.37 AGRR28 pKa = 11.84 KK29 pKa = 8.54 ILNARR34 pKa = 11.84 RR35 pKa = 11.84 ARR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 9.07 SLSAA44 pKa = 3.93
Molecular weight: 5.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.431
IPC2_protein 11.125
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.34
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.076
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.079
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4472
0
4472
1407917
26
18712
314.8
34.2
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.963 ± 0.046
0.919 ± 0.019
6.117 ± 0.043
5.867 ± 0.049
3.699 ± 0.031
8.567 ± 0.063
2.04 ± 0.026
5.353 ± 0.028
3.431 ± 0.039
9.983 ± 0.043
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.896 ± 0.028
2.857 ± 0.029
4.785 ± 0.05
3.438 ± 0.019
6.212 ± 0.056
5.314 ± 0.041
5.679 ± 0.084
7.231 ± 0.039
1.331 ± 0.016
2.318 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here