Rhodobacteraceae bacterium 63075

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; unclassified Rhodobacteraceae

Average proteome isoelectric point is 6.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3352 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A372EY18|A0A372EY18_9RHOB ABC transporter ATP-binding protein OS=Rhodobacteraceae bacterium 63075 OX=2301226 GN=DZK27_03595 PE=4 SV=1
MM1 pKa = 7.93RR2 pKa = 11.84RR3 pKa = 11.84FTPALALVGLLAACGGDD20 pKa = 3.36GTNPFDD26 pKa = 6.24DD27 pKa = 4.39GTTGDD32 pKa = 4.22TGDD35 pKa = 4.11SEE37 pKa = 5.46IPDD40 pKa = 3.92SLAGDD45 pKa = 4.19LQSFSYY51 pKa = 11.04DD52 pKa = 3.28ADD54 pKa = 3.79NQTLTVTGITQDD66 pKa = 3.27SSPISATYY74 pKa = 8.62TRR76 pKa = 11.84NPALDD81 pKa = 3.5TAGYY85 pKa = 8.24EE86 pKa = 4.21AYY88 pKa = 10.49SIQDD92 pKa = 3.55DD93 pKa = 3.98ALSRR97 pKa = 11.84HH98 pKa = 6.36AIALVRR104 pKa = 11.84EE105 pKa = 4.49SGNSGSVRR113 pKa = 11.84AGVVSTGGQFNRR125 pKa = 11.84IHH127 pKa = 6.38HH128 pKa = 6.14GGYY131 pKa = 8.84YY132 pKa = 9.53EE133 pKa = 4.58RR134 pKa = 11.84SGSYY138 pKa = 7.58TQPTTGTARR147 pKa = 11.84YY148 pKa = 8.67AGTYY152 pKa = 10.36AGLTNVQVSGDD163 pKa = 4.16LVPPGPGTPPEE174 pKa = 4.21VLPGQAARR182 pKa = 11.84TEE184 pKa = 4.38GNVLINADD192 pKa = 3.97FNDD195 pKa = 4.08GSIEE199 pKa = 3.78GTIYY203 pKa = 10.75DD204 pKa = 4.38RR205 pKa = 11.84EE206 pKa = 4.23IVDD209 pKa = 3.93TGDD212 pKa = 3.51TLPSIVLVTGEE223 pKa = 4.22IADD226 pKa = 4.36DD227 pKa = 3.8GTFFGEE233 pKa = 4.09EE234 pKa = 3.77VEE236 pKa = 4.38YY237 pKa = 11.25DD238 pKa = 3.36GDD240 pKa = 3.73VDD242 pKa = 5.48NDD244 pKa = 3.11IGDD247 pKa = 3.53YY248 pKa = 11.08GGIFGGPDD256 pKa = 3.21AEE258 pKa = 4.51SVGGVVNLDD267 pKa = 3.56EE268 pKa = 6.02FDD270 pKa = 5.39DD271 pKa = 3.92NTLGFEE277 pKa = 4.6GEE279 pKa = 4.31VEE281 pKa = 4.02TGVFVLEE288 pKa = 4.57SCDD291 pKa = 3.76SADD294 pKa = 3.55ATSPLCPP301 pKa = 4.05

Molecular weight: 31.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A372EYN5|A0A372EYN5_9RHOB MerR family DNA-binding transcriptional regulator OS=Rhodobacteraceae bacterium 63075 OX=2301226 GN=DZK27_04070 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.44RR3 pKa = 11.84TYY5 pKa = 10.31QPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37AGRR28 pKa = 11.84KK29 pKa = 8.41ILNARR34 pKa = 11.84RR35 pKa = 11.84VRR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.13SLSAA44 pKa = 3.93

Molecular weight: 5.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3352

0

3352

1034919

29

2150

308.7

33.59

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.33 ± 0.057

0.889 ± 0.015

5.824 ± 0.036

6.889 ± 0.038

3.753 ± 0.027

8.844 ± 0.047

2.095 ± 0.023

5.036 ± 0.03

3.398 ± 0.035

10.031 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.823 ± 0.023

2.434 ± 0.023

4.886 ± 0.027

3.033 ± 0.025

6.855 ± 0.045

5.242 ± 0.03

5.127 ± 0.031

6.893 ± 0.03

1.369 ± 0.018

2.251 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski