Enterobacterial phage mEp213

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Aguilavirus; Enterobacteria virus mEp213

Average proteome isoelectric point is 6.93

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 74 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K7PH59|K7PH59_9CAUD Tail assembly protein OS=Enterobacterial phage mEp213 OX=1147156 GN=mEp213_017 PE=4 SV=1
MM1 pKa = 7.4LAGMTSSEE9 pKa = 4.2LGDD12 pKa = 2.82WHH14 pKa = 6.96LFYY17 pKa = 10.65RR18 pKa = 11.84EE19 pKa = 5.17HH20 pKa = 6.67YY21 pKa = 9.92FQDD24 pKa = 3.55AQLDD28 pKa = 3.73AHH30 pKa = 6.77FSGLLYY36 pKa = 10.72SISTLFFRR44 pKa = 11.84DD45 pKa = 3.71PEE47 pKa = 4.13LTPAHH52 pKa = 6.63FSLLSPSEE60 pKa = 4.19SVISDD65 pKa = 4.45DD66 pKa = 5.53EE67 pKa = 5.64PDD69 pKa = 5.1DD70 pKa = 4.14DD71 pKa = 4.64TLMTAAEE78 pKa = 5.28GITGGIRR85 pKa = 11.84YY86 pKa = 9.1GPADD90 pKa = 3.26

Molecular weight: 9.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K7PH71|K7PH71_9CAUD Prophage repressor OS=Enterobacterial phage mEp213 OX=1147156 GN=mEp213_052 PE=4 SV=1
MM1 pKa = 7.07TVVITYY7 pKa = 9.95LADD10 pKa = 4.18DD11 pKa = 3.61NARR14 pKa = 11.84NRR16 pKa = 11.84RR17 pKa = 11.84RR18 pKa = 11.84ARR20 pKa = 11.84RR21 pKa = 11.84QAQRR25 pKa = 11.84EE26 pKa = 3.88QAMQEE31 pKa = 3.48QRR33 pKa = 11.84LARR36 pKa = 11.84KK37 pKa = 8.86IALKK41 pKa = 10.84LSGCVRR47 pKa = 11.84ADD49 pKa = 3.17KK50 pKa = 10.69AASLGSLRR58 pKa = 11.84CKK60 pKa = 10.3KK61 pKa = 10.62AEE63 pKa = 3.97EE64 pKa = 4.39CSGSICLPNVAIYY77 pKa = 10.18AAGYY81 pKa = 9.31RR82 pKa = 11.84KK83 pKa = 9.92SKK85 pKa = 10.61QLTARR90 pKa = 3.76

Molecular weight: 10.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

74

0

74

13603

43

1226

183.8

20.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.255 ± 0.423

1.088 ± 0.159

5.83 ± 0.326

6.572 ± 0.462

3.095 ± 0.149

7.27 ± 0.295

1.522 ± 0.192

5.513 ± 0.24

6.08 ± 0.302

7.601 ± 0.259

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.816 ± 0.193

4.425 ± 0.244

3.764 ± 0.276

4.712 ± 0.545

6.138 ± 0.446

6.638 ± 0.34

5.778 ± 0.29

6.219 ± 0.286

1.808 ± 0.201

2.874 ± 0.147
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski