Amino acid dipepetide frequency for Enterobacterial phage mEp213

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.175AlaAla: 11.175 ± 1.219
0.882AlaCys: 0.882 ± 0.267
5.955AlaAsp: 5.955 ± 0.818
7.425AlaGlu: 7.425 ± 1.48
3.602AlaPhe: 3.602 ± 0.657
7.646AlaGly: 7.646 ± 1.097
1.617AlaHis: 1.617 ± 0.38
6.47AlaIle: 6.47 ± 0.753
6.617AlaLys: 6.617 ± 0.9
7.793AlaLeu: 7.793 ± 0.769
3.823AlaMet: 3.823 ± 0.547
4.264AlaAsn: 4.264 ± 0.527
3.014AlaPro: 3.014 ± 0.467
5.073AlaGln: 5.073 ± 1.094
5.661AlaArg: 5.661 ± 0.762
6.249AlaSer: 6.249 ± 0.837
5.367AlaThr: 5.367 ± 0.697
5.587AlaVal: 5.587 ± 0.736
1.838AlaTrp: 1.838 ± 0.358
2.5AlaTyr: 2.5 ± 0.421
0.0AlaXaa: 0.0 ± 0.0
Cys
0.735CysAla: 0.735 ± 0.292
0.147CysCys: 0.147 ± 0.103
0.588CysAsp: 0.588 ± 0.197
0.441CysGlu: 0.441 ± 0.242
0.0CysPhe: 0.0 ± 0.0
1.544CysGly: 1.544 ± 0.324
0.294CysHis: 0.294 ± 0.195
0.662CysIle: 0.662 ± 0.212
1.029CysLys: 1.029 ± 0.324
1.029CysLeu: 1.029 ± 0.263
0.294CysMet: 0.294 ± 0.146
0.294CysAsn: 0.294 ± 0.138
0.588CysPro: 0.588 ± 0.245
0.294CysGln: 0.294 ± 0.166
0.662CysArg: 0.662 ± 0.25
0.956CysSer: 0.956 ± 0.277
0.368CysThr: 0.368 ± 0.159
0.588CysVal: 0.588 ± 0.202
0.147CysTrp: 0.147 ± 0.091
0.221CysTyr: 0.221 ± 0.115
0.0CysXaa: 0.0 ± 0.0
Asp
5.587AspAla: 5.587 ± 0.61
0.662AspCys: 0.662 ± 0.255
3.161AspAsp: 3.161 ± 0.555
4.044AspGlu: 4.044 ± 0.627
1.25AspPhe: 1.25 ± 0.275
6.47AspGly: 6.47 ± 0.832
0.882AspHis: 0.882 ± 0.253
2.72AspIle: 2.72 ± 0.462
3.161AspLys: 3.161 ± 0.459
4.264AspLeu: 4.264 ± 0.596
1.838AspMet: 1.838 ± 0.331
2.794AspAsn: 2.794 ± 0.436
2.059AspPro: 2.059 ± 0.481
1.47AspGln: 1.47 ± 0.31
3.308AspArg: 3.308 ± 0.606
4.117AspSer: 4.117 ± 0.557
2.573AspThr: 2.573 ± 0.477
4.264AspVal: 4.264 ± 0.596
1.323AspTrp: 1.323 ± 0.334
2.353AspTyr: 2.353 ± 0.406
0.0AspXaa: 0.0 ± 0.0
Glu
6.396GluAla: 6.396 ± 1.255
0.515GluCys: 0.515 ± 0.209
3.014GluAsp: 3.014 ± 0.431
5.955GluGlu: 5.955 ± 1.059
1.764GluPhe: 1.764 ± 0.354
4.044GluGly: 4.044 ± 0.52
1.029GluHis: 1.029 ± 0.236
3.382GluIle: 3.382 ± 0.597
4.338GluLys: 4.338 ± 0.63
6.176GluLeu: 6.176 ± 0.767
2.132GluMet: 2.132 ± 0.446
2.5GluAsn: 2.5 ± 0.434
1.691GluPro: 1.691 ± 0.339
3.749GluGln: 3.749 ± 0.647
4.558GluArg: 4.558 ± 0.925
4.632GluSer: 4.632 ± 0.669
3.088GluThr: 3.088 ± 0.465
3.014GluVal: 3.014 ± 0.503
1.47GluTrp: 1.47 ± 0.369
2.279GluTyr: 2.279 ± 0.294
0.0GluXaa: 0.0 ± 0.0
Phe
2.72PheAla: 2.72 ± 0.498
0.588PheCys: 0.588 ± 0.216
2.132PheAsp: 2.132 ± 0.439
1.617PheGlu: 1.617 ± 0.373
0.735PhePhe: 0.735 ± 0.267
2.5PheGly: 2.5 ± 0.489
0.588PheHis: 0.588 ± 0.23
1.764PheIle: 1.764 ± 0.363
0.882PheLys: 0.882 ± 0.252
2.279PheLeu: 2.279 ± 0.414
0.662PheMet: 0.662 ± 0.214
1.323PheAsn: 1.323 ± 0.334
1.103PhePro: 1.103 ± 0.286
0.662PheGln: 0.662 ± 0.22
2.279PheArg: 2.279 ± 0.373
2.573PheSer: 2.573 ± 0.362
2.941PheThr: 2.941 ± 0.427
1.838PheVal: 1.838 ± 0.316
0.809PheTrp: 0.809 ± 0.241
0.956PheTyr: 0.956 ± 0.254
0.0PheXaa: 0.0 ± 0.0
Gly
6.543GlyAla: 6.543 ± 0.727
0.882GlyCys: 0.882 ± 0.217
4.264GlyAsp: 4.264 ± 0.592
3.896GlyGlu: 3.896 ± 0.495
2.5GlyPhe: 2.5 ± 0.499
5.661GlyGly: 5.661 ± 0.973
1.103GlyHis: 1.103 ± 0.233
3.97GlyIle: 3.97 ± 0.483
4.705GlyLys: 4.705 ± 0.682
4.264GlyLeu: 4.264 ± 0.541
3.235GlyMet: 3.235 ± 0.429
4.632GlyAsn: 4.632 ± 0.618
1.029GlyPro: 1.029 ± 0.286
3.088GlyGln: 3.088 ± 0.593
3.602GlyArg: 3.602 ± 0.649
4.264GlySer: 4.264 ± 0.536
4.999GlyThr: 4.999 ± 0.809
5.881GlyVal: 5.881 ± 0.684
2.059GlyTrp: 2.059 ± 0.343
2.132GlyTyr: 2.132 ± 0.384
0.0GlyXaa: 0.0 ± 0.0
His
1.029HisAla: 1.029 ± 0.305
0.294HisCys: 0.294 ± 0.12
0.882HisAsp: 0.882 ± 0.243
0.809HisGlu: 0.809 ± 0.253
0.809HisPhe: 0.809 ± 0.285
1.25HisGly: 1.25 ± 0.325
0.441HisHis: 0.441 ± 0.174
0.735HisIle: 0.735 ± 0.263
1.617HisLys: 1.617 ± 0.398
0.956HisLeu: 0.956 ± 0.306
0.147HisMet: 0.147 ± 0.1
0.221HisAsn: 0.221 ± 0.13
0.809HisPro: 0.809 ± 0.228
0.662HisGln: 0.662 ± 0.227
1.176HisArg: 1.176 ± 0.269
1.25HisSer: 1.25 ± 0.313
0.662HisThr: 0.662 ± 0.234
0.809HisVal: 0.809 ± 0.288
0.294HisTrp: 0.294 ± 0.147
0.368HisTyr: 0.368 ± 0.141
0.0HisXaa: 0.0 ± 0.0
Ile
5.146IleAla: 5.146 ± 0.62
0.882IleCys: 0.882 ± 0.299
4.558IleAsp: 4.558 ± 0.567
3.749IleGlu: 3.749 ± 0.598
1.029IlePhe: 1.029 ± 0.295
3.749IleGly: 3.749 ± 0.514
1.029IleHis: 1.029 ± 0.302
3.308IleIle: 3.308 ± 0.67
3.308IleLys: 3.308 ± 0.52
2.794IleLeu: 2.794 ± 0.441
1.103IleMet: 1.103 ± 0.327
2.5IleAsn: 2.5 ± 0.487
1.764IlePro: 1.764 ± 0.396
2.5IleGln: 2.5 ± 0.535
4.411IleArg: 4.411 ± 0.559
4.338IleSer: 4.338 ± 0.657
4.264IleThr: 4.264 ± 0.563
2.867IleVal: 2.867 ± 0.398
0.809IleTrp: 0.809 ± 0.293
1.029IleTyr: 1.029 ± 0.284
0.0IleXaa: 0.0 ± 0.0
Lys
8.087LysAla: 8.087 ± 1.151
0.515LysCys: 0.515 ± 0.22
2.794LysAsp: 2.794 ± 0.406
4.779LysGlu: 4.779 ± 0.855
1.911LysPhe: 1.911 ± 0.337
3.896LysGly: 3.896 ± 0.441
0.809LysHis: 0.809 ± 0.322
2.647LysIle: 2.647 ± 0.431
3.235LysLys: 3.235 ± 0.532
4.779LysLeu: 4.779 ± 0.673
2.206LysMet: 2.206 ± 0.366
2.647LysAsn: 2.647 ± 0.466
3.455LysPro: 3.455 ± 0.667
2.72LysGln: 2.72 ± 0.528
3.161LysArg: 3.161 ± 0.652
3.455LysSer: 3.455 ± 0.49
4.117LysThr: 4.117 ± 0.556
2.794LysVal: 2.794 ± 0.505
0.809LysTrp: 0.809 ± 0.317
1.985LysTyr: 1.985 ± 0.468
0.0LysXaa: 0.0 ± 0.0
Leu
8.822LeuAla: 8.822 ± 1.136
0.368LeuCys: 0.368 ± 0.218
4.264LeuAsp: 4.264 ± 0.487
5.22LeuGlu: 5.22 ± 0.649
2.206LeuPhe: 2.206 ± 0.384
3.235LeuGly: 3.235 ± 0.475
0.809LeuHis: 0.809 ± 0.293
4.558LeuIle: 4.558 ± 0.474
5.293LeuLys: 5.293 ± 0.731
4.926LeuLeu: 4.926 ± 0.553
1.838LeuMet: 1.838 ± 0.385
4.191LeuAsn: 4.191 ± 0.502
2.867LeuPro: 2.867 ± 0.425
3.529LeuGln: 3.529 ± 0.576
4.705LeuArg: 4.705 ± 0.736
6.47LeuSer: 6.47 ± 0.676
4.926LeuThr: 4.926 ± 0.678
4.632LeuVal: 4.632 ± 0.42
0.809LeuTrp: 0.809 ± 0.287
2.353LeuTyr: 2.353 ± 0.382
0.0LeuXaa: 0.0 ± 0.0
Met
2.5MetAla: 2.5 ± 0.489
0.221MetCys: 0.221 ± 0.115
1.25MetAsp: 1.25 ± 0.308
1.103MetGlu: 1.103 ± 0.293
0.662MetPhe: 0.662 ± 0.197
2.132MetGly: 2.132 ± 0.373
0.368MetHis: 0.368 ± 0.187
1.25MetIle: 1.25 ± 0.3
1.764MetLys: 1.764 ± 0.416
3.014MetLeu: 3.014 ± 0.553
0.882MetMet: 0.882 ± 0.331
0.882MetAsn: 0.882 ± 0.212
1.764MetPro: 1.764 ± 0.293
1.323MetGln: 1.323 ± 0.313
1.764MetArg: 1.764 ± 0.36
2.867MetSer: 2.867 ± 0.482
1.985MetThr: 1.985 ± 0.416
1.617MetVal: 1.617 ± 0.283
0.221MetTrp: 0.221 ± 0.134
0.588MetTyr: 0.588 ± 0.237
0.0MetXaa: 0.0 ± 0.0
Asn
4.411AsnAla: 4.411 ± 0.5
0.515AsnCys: 0.515 ± 0.169
2.279AsnAsp: 2.279 ± 0.517
2.132AsnGlu: 2.132 ± 0.446
1.103AsnPhe: 1.103 ± 0.395
4.705AsnGly: 4.705 ± 0.903
1.029AsnHis: 1.029 ± 0.346
3.529AsnIle: 3.529 ± 0.509
2.5AsnLys: 2.5 ± 0.531
3.088AsnLeu: 3.088 ± 0.454
0.662AsnMet: 0.662 ± 0.24
1.397AsnAsn: 1.397 ± 0.318
2.426AsnPro: 2.426 ± 0.46
2.941AsnGln: 2.941 ± 0.494
1.911AsnArg: 1.911 ± 0.418
2.72AsnSer: 2.72 ± 0.47
2.353AsnThr: 2.353 ± 0.537
1.985AsnVal: 1.985 ± 0.438
0.515AsnTrp: 0.515 ± 0.194
2.059AsnTyr: 2.059 ± 0.4
0.0AsnXaa: 0.0 ± 0.0
Pro
4.926ProAla: 4.926 ± 0.673
0.515ProCys: 0.515 ± 0.208
2.5ProAsp: 2.5 ± 0.429
3.088ProGlu: 3.088 ± 0.557
1.25ProPhe: 1.25 ± 0.311
2.794ProGly: 2.794 ± 0.513
0.662ProHis: 0.662 ± 0.191
0.882ProIle: 0.882 ± 0.275
2.573ProLys: 2.573 ± 0.424
2.573ProLeu: 2.573 ± 0.353
1.176ProMet: 1.176 ± 0.36
1.544ProAsn: 1.544 ± 0.416
1.176ProPro: 1.176 ± 0.281
1.47ProGln: 1.47 ± 0.335
1.911ProArg: 1.911 ± 0.427
1.911ProSer: 1.911 ± 0.347
1.617ProThr: 1.617 ± 0.397
3.235ProVal: 3.235 ± 0.458
0.735ProTrp: 0.735 ± 0.229
1.103ProTyr: 1.103 ± 0.265
0.0ProXaa: 0.0 ± 0.0
Gln
5.22GlnAla: 5.22 ± 1.105
0.588GlnCys: 0.588 ± 0.186
1.985GlnAsp: 1.985 ± 0.419
3.235GlnGlu: 3.235 ± 0.443
1.544GlnPhe: 1.544 ± 0.421
2.573GlnGly: 2.573 ± 0.465
0.662GlnHis: 0.662 ± 0.198
2.573GlnIle: 2.573 ± 0.404
2.5GlnLys: 2.5 ± 0.588
4.632GlnLeu: 4.632 ± 1.177
1.397GlnMet: 1.397 ± 0.289
1.176GlnAsn: 1.176 ± 0.331
1.176GlnPro: 1.176 ± 0.262
3.602GlnGln: 3.602 ± 1.447
3.382GlnArg: 3.382 ± 0.6
2.647GlnSer: 2.647 ± 0.471
3.161GlnThr: 3.161 ± 0.651
3.161GlnVal: 3.161 ± 0.436
0.956GlnTrp: 0.956 ± 0.268
0.956GlnTyr: 0.956 ± 0.309
0.0GlnXaa: 0.0 ± 0.0
Arg
5.22ArgAla: 5.22 ± 0.639
0.956ArgCys: 0.956 ± 0.335
2.867ArgAsp: 2.867 ± 0.59
4.999ArgGlu: 4.999 ± 0.759
1.397ArgPhe: 1.397 ± 0.27
3.235ArgGly: 3.235 ± 0.501
1.397ArgHis: 1.397 ± 0.338
3.823ArgIle: 3.823 ± 0.561
4.999ArgLys: 4.999 ± 0.746
5.293ArgLeu: 5.293 ± 0.799
1.764ArgMet: 1.764 ± 0.372
2.647ArgAsn: 2.647 ± 0.62
1.985ArgPro: 1.985 ± 0.558
3.749ArgGln: 3.749 ± 0.633
4.852ArgArg: 4.852 ± 1.021
3.014ArgSer: 3.014 ± 0.548
2.647ArgThr: 2.647 ± 0.478
3.676ArgVal: 3.676 ± 0.558
1.103ArgTrp: 1.103 ± 0.31
1.691ArgTyr: 1.691 ± 0.393
0.0ArgXaa: 0.0 ± 0.0
Ser
7.205SerAla: 7.205 ± 0.809
0.441SerCys: 0.441 ± 0.171
4.926SerAsp: 4.926 ± 0.53
4.044SerGlu: 4.044 ± 0.974
2.206SerPhe: 2.206 ± 0.421
5.514SerGly: 5.514 ± 0.683
0.515SerHis: 0.515 ± 0.161
3.014SerIle: 3.014 ± 0.55
3.455SerLys: 3.455 ± 0.63
5.514SerLeu: 5.514 ± 0.481
2.059SerMet: 2.059 ± 0.352
3.161SerAsn: 3.161 ± 0.592
2.5SerPro: 2.5 ± 0.522
3.308SerGln: 3.308 ± 0.56
3.455SerArg: 3.455 ± 0.738
4.338SerSer: 4.338 ± 0.653
2.279SerThr: 2.279 ± 0.436
5.881SerVal: 5.881 ± 0.563
1.323SerTrp: 1.323 ± 0.334
1.25SerTyr: 1.25 ± 0.285
0.0SerXaa: 0.0 ± 0.0
Thr
6.764ThrAla: 6.764 ± 0.937
0.588ThrCys: 0.588 ± 0.213
4.117ThrAsp: 4.117 ± 0.478
3.455ThrGlu: 3.455 ± 0.365
2.573ThrPhe: 2.573 ± 0.451
4.926ThrGly: 4.926 ± 0.665
0.515ThrHis: 0.515 ± 0.196
3.235ThrIle: 3.235 ± 0.482
2.72ThrLys: 2.72 ± 0.45
3.896ThrLeu: 3.896 ± 0.745
0.294ThrMet: 0.294 ± 0.142
1.838ThrAsn: 1.838 ± 0.395
3.602ThrPro: 3.602 ± 0.627
1.911ThrGln: 1.911 ± 0.391
2.941ThrArg: 2.941 ± 0.467
3.235ThrSer: 3.235 ± 0.636
3.749ThrThr: 3.749 ± 0.592
4.338ThrVal: 4.338 ± 0.899
1.544ThrTrp: 1.544 ± 0.322
1.544ThrTyr: 1.544 ± 0.321
0.0ThrXaa: 0.0 ± 0.0
Val
6.69ValAla: 6.69 ± 0.87
0.588ValCys: 0.588 ± 0.242
3.161ValAsp: 3.161 ± 0.386
3.308ValGlu: 3.308 ± 0.507
2.647ValPhe: 2.647 ± 0.437
4.044ValGly: 4.044 ± 0.549
0.956ValHis: 0.956 ± 0.234
3.823ValIle: 3.823 ± 0.469
3.455ValLys: 3.455 ± 0.427
4.117ValLeu: 4.117 ± 0.443
1.544ValMet: 1.544 ± 0.358
3.749ValAsn: 3.749 ± 0.674
2.72ValPro: 2.72 ± 0.508
2.794ValGln: 2.794 ± 0.548
3.823ValArg: 3.823 ± 0.492
4.117ValSer: 4.117 ± 0.605
4.485ValThr: 4.485 ± 0.81
3.896ValVal: 3.896 ± 0.585
0.515ValTrp: 0.515 ± 0.185
1.764ValTyr: 1.764 ± 0.369
0.0ValXaa: 0.0 ± 0.0
Trp
1.029TrpAla: 1.029 ± 0.216
0.368TrpCys: 0.368 ± 0.196
1.029TrpAsp: 1.029 ± 0.262
0.735TrpGlu: 0.735 ± 0.26
1.029TrpPhe: 1.029 ± 0.319
1.176TrpGly: 1.176 ± 0.255
0.368TrpHis: 0.368 ± 0.14
1.25TrpIle: 1.25 ± 0.339
1.176TrpLys: 1.176 ± 0.276
1.911TrpLeu: 1.911 ± 0.513
0.515TrpMet: 0.515 ± 0.168
0.662TrpAsn: 0.662 ± 0.295
0.735TrpPro: 0.735 ± 0.303
0.735TrpGln: 0.735 ± 0.2
0.882TrpArg: 0.882 ± 0.285
1.617TrpSer: 1.617 ± 0.391
0.809TrpThr: 0.809 ± 0.25
1.029TrpVal: 1.029 ± 0.228
0.441TrpTrp: 0.441 ± 0.187
0.588TrpTyr: 0.588 ± 0.179
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.353TyrAla: 2.353 ± 0.509
0.368TyrCys: 0.368 ± 0.166
2.573TyrAsp: 2.573 ± 0.495
1.691TyrGlu: 1.691 ± 0.444
0.735TyrPhe: 0.735 ± 0.234
1.617TyrGly: 1.617 ± 0.332
0.147TyrHis: 0.147 ± 0.109
1.544TyrIle: 1.544 ± 0.327
1.47TyrLys: 1.47 ± 0.3
2.72TyrLeu: 2.72 ± 0.335
0.515TyrMet: 0.515 ± 0.177
1.838TyrAsn: 1.838 ± 0.326
1.176TyrPro: 1.176 ± 0.276
1.544TyrGln: 1.544 ± 0.277
2.941TyrArg: 2.941 ± 0.438
1.617TyrSer: 1.617 ± 0.347
1.397TyrThr: 1.397 ± 0.264
1.103TyrVal: 1.103 ± 0.344
0.368TyrTrp: 0.368 ± 0.141
1.029TyrTyr: 1.029 ± 0.353
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 74 proteins (13603 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski