Lentzea aerocolonigenes (Lechevalieria aerocolonigenes) (Saccharothrix aerocolonigenes)
Average proteome isoelectric point is 6.19
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8237 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0F0GTK8|A0A0F0GTK8_LENAE ABC transporter ATP-binding protein OS=Lentzea aerocolonigenes OX=68170 GN=UK23_26465 PE=4 SV=1
MM1 pKa = 8.04 SEE3 pKa = 4.12 DD4 pKa = 4.6 DD5 pKa = 3.71 EE6 pKa = 6.38 DD7 pKa = 4.86 EE8 pKa = 4.25 FAEE11 pKa = 5.26 DD12 pKa = 5.66 DD13 pKa = 4.05 DD14 pKa = 6.46 FEE16 pKa = 6.25 DD17 pKa = 5.9 EE18 pKa = 4.7 EE19 pKa = 5.37 DD20 pKa = 4.16 ADD22 pKa = 4.73 DD23 pKa = 5.04 LFAVTPEE30 pKa = 3.82 PRR32 pKa = 11.84 TVCHH36 pKa = 6.43 LCGGASYY43 pKa = 10.45 IAAPTLAIIGGAPRR57 pKa = 11.84 TVDD60 pKa = 3.11 NGRR63 pKa = 11.84 EE64 pKa = 4.07 CPHH67 pKa = 6.82 CSGARR72 pKa = 11.84 TFNGLVPPLL81 pKa = 3.82
Molecular weight: 8.66 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.75
IPC2_protein 3.77
IPC_protein 3.719
Toseland 3.516
ProMoST 3.897
Dawson 3.719
Bjellqvist 3.872
Wikipedia 3.656
Rodwell 3.554
Grimsley 3.439
Solomon 3.694
Lehninger 3.656
Nozaki 3.846
DTASelect 4.037
Thurlkill 3.579
EMBOSS 3.668
Sillero 3.846
Patrickios 1.863
IPC_peptide 3.694
IPC2_peptide 3.821
IPC2.peptide.svr19 3.777
Protein with the highest isoelectric point:
>tr|A0A0F0GWX4|A0A0F0GWX4_LENAE Uncharacterized protein OS=Lentzea aerocolonigenes OX=68170 GN=UK23_23145 PE=4 SV=1
MM1 pKa = 7.53 SKK3 pKa = 10.53 GKK5 pKa = 8.66 RR6 pKa = 11.84 TFQPNNRR13 pKa = 11.84 RR14 pKa = 11.84 RR15 pKa = 11.84 AKK17 pKa = 8.7 THH19 pKa = 5.15 GFRR22 pKa = 11.84 LRR24 pKa = 11.84 MRR26 pKa = 11.84 TRR28 pKa = 11.84 AGRR31 pKa = 11.84 AILAARR37 pKa = 11.84 RR38 pKa = 11.84 SKK40 pKa = 10.82 GRR42 pKa = 11.84 ATLSAA47 pKa = 4.08
Molecular weight: 5.4 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.286
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.042
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.676
Grimsley 13.086
Solomon 13.539
Lehninger 13.437
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.539
Sillero 13.042
Patrickios 12.398
IPC_peptide 13.539
IPC2_peptide 12.53
IPC2.peptide.svr19 9.215
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
8237
0
8237
2596602
32
10826
315.2
33.96
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.389 ± 0.034
0.804 ± 0.009
5.993 ± 0.023
5.636 ± 0.027
3.093 ± 0.015
8.866 ± 0.026
2.239 ± 0.012
3.639 ± 0.018
2.622 ± 0.021
10.305 ± 0.035
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.837 ± 0.01
2.315 ± 0.021
5.505 ± 0.02
3.089 ± 0.014
7.194 ± 0.031
5.59 ± 0.019
6.064 ± 0.025
9.139 ± 0.029
1.618 ± 0.013
2.063 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here