Ailuropoda melanoleuca papillomavirus 4
Average proteome isoelectric point is 6.61
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A220IGG4|A0A220IGG4_9PAPI Major capsid protein L1 OS=Ailuropoda melanoleuca papillomavirus 4 OX=2016453 GN=L1 PE=3 SV=1
MM1 pKa = 7.54 IGKK4 pKa = 8.5 QATLCDD10 pKa = 3.48 IVLTEE15 pKa = 3.86 QAEE18 pKa = 4.34 AVDD21 pKa = 4.32 LLCYY25 pKa = 10.18 EE26 pKa = 4.2 QMPSDD31 pKa = 3.59 EE32 pKa = 4.96 EE33 pKa = 4.25 EE34 pKa = 4.3 EE35 pKa = 4.1 QEE37 pKa = 4.23 HH38 pKa = 6.6 RR39 pKa = 11.84 DD40 pKa = 4.06 FYY42 pKa = 11.29 RR43 pKa = 11.84 VAIDD47 pKa = 4.7 CATCKK52 pKa = 10.39 NRR54 pKa = 11.84 LHH56 pKa = 6.35 FVCAANGEE64 pKa = 4.66 DD65 pKa = 3.44 ILKK68 pKa = 10.65 LEE70 pKa = 4.47 EE71 pKa = 4.29 LLFTLGFLCSSCVKK85 pKa = 10.0 TNRR88 pKa = 11.84 LNHH91 pKa = 6.26 GGG93 pKa = 3.46
Molecular weight: 10.5 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.31
IPC2_protein 4.622
IPC_protein 4.495
Toseland 4.355
ProMoST 4.596
Dawson 4.431
Bjellqvist 4.584
Wikipedia 4.291
Rodwell 4.342
Grimsley 4.266
Solomon 4.431
Lehninger 4.38
Nozaki 4.546
DTASelect 4.66
Thurlkill 4.355
EMBOSS 4.317
Sillero 4.609
Patrickios 2.015
IPC_peptide 4.431
IPC2_peptide 4.596
IPC2.peptide.svr19 4.533
Protein with the highest isoelectric point:
>tr|A0A220IGF9|A0A220IGF9_9PAPI Replication protein E1 OS=Ailuropoda melanoleuca papillomavirus 4 OX=2016453 GN=E1 PE=3 SV=1
MM1 pKa = 7.04 EE2 pKa = 5.05 TLNEE6 pKa = 4.03 LLDD9 pKa = 4.0 YY10 pKa = 11.32 VQDD13 pKa = 3.68 QLLILYY19 pKa = 7.34 EE20 pKa = 4.26 TNSNKK25 pKa = 9.69 IADD28 pKa = 4.27 QIKK31 pKa = 9.15 HH32 pKa = 3.79 WHH34 pKa = 6.44 LNRR37 pKa = 11.84 KK38 pKa = 9.06 EE39 pKa = 4.09 NVLLYY44 pKa = 9.13 YY45 pKa = 10.42 ARR47 pKa = 11.84 KK48 pKa = 10.13 NGITRR53 pKa = 11.84 LGMRR57 pKa = 11.84 PIPSLQVSHH66 pKa = 6.02 QKK68 pKa = 10.27 ARR70 pKa = 11.84 AAIEE74 pKa = 4.04 QEE76 pKa = 5.0 LMLQSLGKK84 pKa = 10.75 SNFGSEE90 pKa = 3.69 KK91 pKa = 8.82 WTLQDD96 pKa = 3.24 TSKK99 pKa = 10.87 EE100 pKa = 3.92 RR101 pKa = 11.84 LLAEE105 pKa = 4.01 PRR107 pKa = 11.84 YY108 pKa = 9.68 CFKK111 pKa = 11.05 KK112 pKa = 10.06 GGKK115 pKa = 9.42 EE116 pKa = 3.04 IDD118 pKa = 3.18 VRR120 pKa = 11.84 FDD122 pKa = 3.33 NKK124 pKa = 10.9 AEE126 pKa = 4.34 NITRR130 pKa = 11.84 YY131 pKa = 7.5 TLWDD135 pKa = 3.89 WIYY138 pKa = 11.24 YY139 pKa = 9.8 QDD141 pKa = 6.31 DD142 pKa = 3.37 NDD144 pKa = 3.54 KK145 pKa = 9.14 WQKK148 pKa = 9.66 TEE150 pKa = 3.91 GQVDD154 pKa = 3.78 EE155 pKa = 5.64 RR156 pKa = 11.84 GLFYY160 pKa = 10.9 LDD162 pKa = 3.22 QAGVKK167 pKa = 9.46 VYY169 pKa = 10.05 YY170 pKa = 11.01 VNFEE174 pKa = 4.25 EE175 pKa = 4.82 EE176 pKa = 4.12 SKK178 pKa = 10.96 KK179 pKa = 10.54 YY180 pKa = 10.62 GKK182 pKa = 8.53 TGAYY186 pKa = 8.1 EE187 pKa = 4.13 VLHH190 pKa = 6.69 KK191 pKa = 9.73 LTKK194 pKa = 10.18 NAISTSSFRR203 pKa = 11.84 SSSGGEE209 pKa = 4.19 SEE211 pKa = 5.15 HH212 pKa = 6.41 PTSSGLSSRR221 pKa = 11.84 HH222 pKa = 3.56 STPRR226 pKa = 11.84 KK227 pKa = 9.31 KK228 pKa = 9.72 IHH230 pKa = 5.84 SPGRR234 pKa = 11.84 RR235 pKa = 11.84 TRR237 pKa = 11.84 RR238 pKa = 11.84 YY239 pKa = 8.82 RR240 pKa = 11.84 RR241 pKa = 11.84 RR242 pKa = 11.84 ASPCVSRR249 pKa = 11.84 GRR251 pKa = 11.84 RR252 pKa = 11.84 EE253 pKa = 3.91 RR254 pKa = 11.84 EE255 pKa = 4.07 SPPKK259 pKa = 9.2 EE260 pKa = 3.84 HH261 pKa = 7.37 PARR264 pKa = 11.84 RR265 pKa = 11.84 LLPPSPGEE273 pKa = 3.92 VGGCHH278 pKa = 5.68 HH279 pKa = 7.51 TSQAGAGGRR288 pKa = 11.84 LEE290 pKa = 4.94 RR291 pKa = 11.84 LVQEE295 pKa = 4.84 ARR297 pKa = 11.84 DD298 pKa = 3.85 PPLLVLGGNPNSLKK312 pKa = 10.44 CLRR315 pKa = 11.84 FRR317 pKa = 11.84 LRR319 pKa = 11.84 KK320 pKa = 9.99 GYY322 pKa = 10.63 GGLFDD327 pKa = 4.16 TVSTTWQWTNVRR339 pKa = 11.84 DD340 pKa = 3.74 PKK342 pKa = 10.62 VNHH345 pKa = 6.41 RR346 pKa = 11.84 MLVAFTDD353 pKa = 3.78 TQQRR357 pKa = 11.84 EE358 pKa = 4.09 QFLLKK363 pKa = 10.65 VPLPKK368 pKa = 10.17 SVSHH372 pKa = 6.69 FFGSFNGLL380 pKa = 3.24
Molecular weight: 43.77 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.132
IPC2_protein 9.194
IPC_protein 9.18
Toseland 9.882
ProMoST 9.589
Dawson 10.101
Bjellqvist 9.794
Wikipedia 10.277
Rodwell 10.467
Grimsley 10.175
Solomon 10.131
Lehninger 10.087
Nozaki 9.911
DTASelect 9.78
Thurlkill 9.955
EMBOSS 10.292
Sillero 10.028
Patrickios 9.706
IPC_peptide 10.131
IPC2_peptide 8.404
IPC2.peptide.svr19 8.153
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
2329
93
596
332.7
37.75
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.41 ± 0.579
2.362 ± 0.862
5.882 ± 0.452
6.655 ± 0.57
5.067 ± 0.416
6.183 ± 0.672
2.319 ± 0.459
4.809 ± 0.851
6.526 ± 0.897
9.704 ± 0.738
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.374 ± 0.39
4.208 ± 0.442
5.582 ± 1.087
4.079 ± 0.344
6.226 ± 0.637
7.6 ± 0.807
5.925 ± 0.583
5.539 ± 0.709
1.331 ± 0.372
3.22 ± 0.226
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here