Indian citrus ringspot virus (isolate Kinnow mandarin/India/K1/1996) (ICRSV)

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Tymovirales; Alphaflexiviridae; Potexvirus; Mandarivirus; Indian citrus ringspot virus

Average proteome isoelectric point is 7.08

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q918W3|RDRP_ICRSV RNA replication protein OS=Indian citrus ringspot virus (isolate Kinnow mandarin/India/K1/1996) OX=651357 GN=ORF1 PE=3 SV=1
MM1 pKa = 8.0DD2 pKa = 4.34FAEE5 pKa = 4.55LLEE8 pKa = 4.69SKK10 pKa = 10.8AFTRR14 pKa = 11.84TRR16 pKa = 11.84LPLSKK21 pKa = 9.93PIVVHH26 pKa = 6.53AVAGAGKK33 pKa = 8.62TSLLEE38 pKa = 3.76NYY40 pKa = 10.21ARR42 pKa = 11.84INPAARR48 pKa = 11.84IYY50 pKa = 10.62TPIAQQSNSLLLSPFTQSLEE70 pKa = 3.86QADD73 pKa = 3.98IVDD76 pKa = 5.06EE77 pKa = 4.59YY78 pKa = 10.69PLSTLHH84 pKa = 6.88PGVEE88 pKa = 4.38YY89 pKa = 11.18VLADD93 pKa = 4.6PIQYY97 pKa = 10.49LGSKK101 pKa = 10.38DD102 pKa = 3.79LLKK105 pKa = 10.45PHH107 pKa = 6.73YY108 pKa = 9.82ICPTTHH114 pKa = 7.23RR115 pKa = 11.84FGHH118 pKa = 5.34STAEE122 pKa = 4.1FLTSLGIEE130 pKa = 4.67TYY132 pKa = 9.95AHH134 pKa = 6.51KK135 pKa = 10.08PDD137 pKa = 4.13RR138 pKa = 11.84LTIANIFKK146 pKa = 9.79TEE148 pKa = 3.69PHH150 pKa = 6.07GQVIACDD157 pKa = 4.27LDD159 pKa = 4.03TQQLAARR166 pKa = 11.84HH167 pKa = 5.56SLDD170 pKa = 3.35YY171 pKa = 11.01LRR173 pKa = 11.84PCQSIGKK180 pKa = 6.12TFKK183 pKa = 10.24DD184 pKa = 3.3TTILISHH191 pKa = 6.91EE192 pKa = 4.36LNRR195 pKa = 11.84DD196 pKa = 3.39TLTKK200 pKa = 10.04EE201 pKa = 3.56IYY203 pKa = 9.86IALTRR208 pKa = 11.84HH209 pKa = 5.4TNSVTILTPDD219 pKa = 3.44APSTSSS225 pKa = 2.88

Molecular weight: 24.95 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q918W2|TGB1_ICRSV Movement and silencing protein TGBp1 OS=Indian citrus ringspot virus (isolate Kinnow mandarin/India/K1/1996) OX=651357 GN=ORF2 PE=3 SV=1
MM1 pKa = 7.9PLQPPPDD8 pKa = 3.75HH9 pKa = 6.28TWAVRR14 pKa = 11.84IIALGLAVTALIFTSTRR31 pKa = 11.84DD32 pKa = 3.48TSRR35 pKa = 11.84HH36 pKa = 5.34VGDD39 pKa = 5.07PSHH42 pKa = 6.68SLPFGGHH49 pKa = 5.16YY50 pKa = 9.98RR51 pKa = 11.84DD52 pKa = 3.68GSKK55 pKa = 10.45VIHH58 pKa = 6.1YY59 pKa = 7.61NSPRR63 pKa = 11.84SSKK66 pKa = 10.31PSNHH70 pKa = 5.59TPYY73 pKa = 11.29LLFAPIGIILLIHH86 pKa = 6.51ALHH89 pKa = 6.78RR90 pKa = 11.84LGNSAHH96 pKa = 6.97ICRR99 pKa = 11.84CTHH102 pKa = 6.46CMPHH106 pKa = 5.92SQTT109 pKa = 3.84

Molecular weight: 12.02 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

2599

60

1658

433.2

48.64

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.119 ± 0.668

2.193 ± 0.427

5.194 ± 0.286

5.04 ± 0.75

3.694 ± 0.347

4.502 ± 0.29

4.04 ± 0.597

5.387 ± 0.61

5.04 ± 0.679

9.504 ± 0.973

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.616 ± 0.289

4.656 ± 0.677

7.734 ± 1.64

4.117 ± 0.711

5.541 ± 1.047

6.272 ± 0.844

8.195 ± 0.889

4.656 ± 0.381

1.308 ± 0.31

3.194 ± 0.232
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski