Flavobacterium psychrophilum (strain ATCC 49511 / DSM 21280 / CIP 103535 / JIP02/86)

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Flavobacterium; Flavobacterium psychrophilum

Average proteome isoelectric point is 6.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2421 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A6H2A2|A6H2A2_FLAPJ Probable DNA/RNA non-specific endonuclease OS=Flavobacterium psychrophilum (strain ATCC 49511 / DSM 21280 / CIP 103535 / JIP02/86) OX=402612 GN=nucA PE=4 SV=1
MM1 pKa = 7.36QDD3 pKa = 2.7ITIKK7 pKa = 9.38ITDD10 pKa = 3.77RR11 pKa = 11.84EE12 pKa = 4.67GVSHH16 pKa = 6.82EE17 pKa = 4.28VQAPTDD23 pKa = 3.26MSMNIMEE30 pKa = 5.15LVRR33 pKa = 11.84MYY35 pKa = 10.97EE36 pKa = 4.08LVPEE40 pKa = 4.35GTIGICGGMAMCASCQCYY58 pKa = 10.14VLNDD62 pKa = 3.45VVLPEE67 pKa = 5.1IGDD70 pKa = 3.89DD71 pKa = 3.55EE72 pKa = 4.89GAMLSEE78 pKa = 4.16AFNVKK83 pKa = 9.72EE84 pKa = 3.93NSRR87 pKa = 11.84LGCQIQITTEE97 pKa = 3.52IDD99 pKa = 3.25GLEE102 pKa = 4.01IEE104 pKa = 5.06LAPEE108 pKa = 3.99SS109 pKa = 3.93

Molecular weight: 11.9 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A6GZ97|A6GZ97_FLAPJ 50S ribosomal protein L23 OS=Flavobacterium psychrophilum (strain ATCC 49511 / DSM 21280 / CIP 103535 / JIP02/86) OX=402612 GN=rplW PE=3 SV=1
MM1 pKa = 7.54SVRR4 pKa = 11.84KK5 pKa = 9.68LKK7 pKa = 10.5PITPGQRR14 pKa = 11.84FRR16 pKa = 11.84VVNGFDD22 pKa = 5.49AITTDD27 pKa = 3.09KK28 pKa = 11.02PEE30 pKa = 4.34RR31 pKa = 11.84SLIAPIKK38 pKa = 10.47NSGGRR43 pKa = 11.84NSQGKK48 pKa = 6.33MTMRR52 pKa = 11.84YY53 pKa = 7.39TGGGHH58 pKa = 4.86KK59 pKa = 9.44QRR61 pKa = 11.84YY62 pKa = 8.74RR63 pKa = 11.84IIDD66 pKa = 3.81FKK68 pKa = 10.3RR69 pKa = 11.84TKK71 pKa = 10.53DD72 pKa = 3.74GIPASVKK79 pKa = 10.54SIEE82 pKa = 4.0YY83 pKa = 10.38DD84 pKa = 3.37PNRR87 pKa = 11.84TAFIALLAYY96 pKa = 10.31ADD98 pKa = 3.96GEE100 pKa = 4.24KK101 pKa = 10.14TYY103 pKa = 10.54IIAQNGLQVGQKK115 pKa = 9.1VVSGEE120 pKa = 3.88GSAPEE125 pKa = 4.11IGNTLPLSKK134 pKa = 10.28IPLGTVISCIEE145 pKa = 3.9LRR147 pKa = 11.84PGQGAVIARR156 pKa = 11.84SAGTFAQLMARR167 pKa = 11.84DD168 pKa = 4.06GKK170 pKa = 10.75YY171 pKa = 9.05ATIKK175 pKa = 9.6MPSGEE180 pKa = 4.18TRR182 pKa = 11.84LILLTCSATIGAVSNSDD199 pKa = 2.96HH200 pKa = 6.02QLIVSGKK207 pKa = 9.19AGRR210 pKa = 11.84TRR212 pKa = 11.84WLGRR216 pKa = 11.84RR217 pKa = 11.84PRR219 pKa = 11.84TRR221 pKa = 11.84PVAMNPVDD229 pKa = 3.63HH230 pKa = 7.13PMGGGEE236 pKa = 4.08GRR238 pKa = 11.84SSGGHH243 pKa = 4.7PRR245 pKa = 11.84SRR247 pKa = 11.84NGIPAKK253 pKa = 10.29GYY255 pKa = 7.29RR256 pKa = 11.84TRR258 pKa = 11.84SKK260 pKa = 10.85KK261 pKa = 10.4NPSNKK266 pKa = 10.11YY267 pKa = 8.41IVEE270 pKa = 4.07RR271 pKa = 11.84RR272 pKa = 11.84KK273 pKa = 10.24KK274 pKa = 10.1

Molecular weight: 29.71 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2421

0

2421

806156

25

3325

333.0

37.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.294 ± 0.061

0.817 ± 0.019

5.007 ± 0.038

6.33 ± 0.059

5.41 ± 0.047

6.014 ± 0.053

1.654 ± 0.024

8.798 ± 0.056

8.571 ± 0.073

9.116 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.152 ± 0.026

6.827 ± 0.052

3.22 ± 0.035

3.489 ± 0.028

3.011 ± 0.031

6.382 ± 0.042

6.082 ± 0.091

5.926 ± 0.051

0.917 ± 0.018

3.985 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski