Amino acid dipepetide frequency for Flavobacterium psychrophilum (strain ATCC 49511 / DSM 21280 / CIP 103535 / JIP02/86)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.766AlaAla: 3.766 ± 0.1
0.556AlaCys: 0.556 ± 0.03
2.92AlaAsp: 2.92 ± 0.1
3.667AlaGlu: 3.667 ± 0.108
3.302AlaPhe: 3.302 ± 0.07
4.064AlaGly: 4.064 ± 0.117
1.023AlaHis: 1.023 ± 0.037
5.848AlaIle: 5.848 ± 0.093
5.524AlaLys: 5.524 ± 0.125
5.738AlaLeu: 5.738 ± 0.098
1.487AlaMet: 1.487 ± 0.05
4.066AlaAsn: 4.066 ± 0.076
1.717AlaPro: 1.717 ± 0.069
2.612AlaGln: 2.612 ± 0.062
1.816AlaArg: 1.816 ± 0.044
4.058AlaSer: 4.058 ± 0.083
4.235AlaThr: 4.235 ± 0.167
3.89AlaVal: 3.89 ± 0.088
0.546AlaTrp: 0.546 ± 0.028
2.109AlaTyr: 2.109 ± 0.055
0.0AlaXaa: 0.0 ± 0.0
Cys
0.497CysAla: 0.497 ± 0.037
0.092CysCys: 0.092 ± 0.011
0.471CysAsp: 0.471 ± 0.035
0.46CysGlu: 0.46 ± 0.026
0.479CysPhe: 0.479 ± 0.029
0.617CysGly: 0.617 ± 0.035
0.21CysHis: 0.21 ± 0.019
0.629CysIle: 0.629 ± 0.032
0.605CysLys: 0.605 ± 0.032
0.672CysLeu: 0.672 ± 0.03
0.15CysMet: 0.15 ± 0.013
0.481CysAsn: 0.481 ± 0.028
0.344CysPro: 0.344 ± 0.03
0.259CysGln: 0.259 ± 0.023
0.186CysArg: 0.186 ± 0.014
0.656CysSer: 0.656 ± 0.041
0.52CysThr: 0.52 ± 0.044
0.471CysVal: 0.471 ± 0.025
0.063CysTrp: 0.063 ± 0.008
0.304CysTyr: 0.304 ± 0.02
0.0CysXaa: 0.0 ± 0.0
Asp
3.367AspAla: 3.367 ± 0.09
0.424AspCys: 0.424 ± 0.024
2.433AspAsp: 2.433 ± 0.074
3.193AspGlu: 3.193 ± 0.068
3.612AspPhe: 3.612 ± 0.07
3.012AspGly: 3.012 ± 0.071
0.698AspHis: 0.698 ± 0.028
4.209AspIle: 4.209 ± 0.075
4.624AspLys: 4.624 ± 0.088
4.813AspLeu: 4.813 ± 0.086
1.021AspMet: 1.021 ± 0.035
3.042AspAsn: 3.042 ± 0.068
1.231AspPro: 1.231 ± 0.047
1.306AspGln: 1.306 ± 0.044
1.494AspArg: 1.494 ± 0.049
2.765AspSer: 2.765 ± 0.068
2.47AspThr: 2.47 ± 0.06
3.022AspVal: 3.022 ± 0.068
0.625AspTrp: 0.625 ± 0.027
2.707AspTyr: 2.707 ± 0.058
0.0AspXaa: 0.0 ± 0.0
Glu
4.214GluAla: 4.214 ± 0.096
0.362GluCys: 0.362 ± 0.021
2.853GluAsp: 2.853 ± 0.064
4.273GluGlu: 4.273 ± 0.096
2.821GluPhe: 2.821 ± 0.059
3.234GluGly: 3.234 ± 0.065
0.956GluHis: 0.956 ± 0.035
6.444GluIle: 6.444 ± 0.108
6.677GluLys: 6.677 ± 0.128
5.573GluLeu: 5.573 ± 0.091
1.579GluMet: 1.579 ± 0.051
5.334GluAsn: 5.334 ± 0.109
1.394GluPro: 1.394 ± 0.044
2.084GluGln: 2.084 ± 0.061
2.088GluArg: 2.088 ± 0.064
3.132GluSer: 3.132 ± 0.06
3.636GluThr: 3.636 ± 0.067
3.891GluVal: 3.891 ± 0.072
0.485GluTrp: 0.485 ± 0.025
2.264GluTyr: 2.264 ± 0.055
0.0GluXaa: 0.0 ± 0.0
Phe
3.339PheAla: 3.339 ± 0.074
0.528PheCys: 0.528 ± 0.027
3.283PheAsp: 3.283 ± 0.058
3.553PheGlu: 3.553 ± 0.073
2.895PhePhe: 2.895 ± 0.088
3.649PheGly: 3.649 ± 0.085
0.84PheHis: 0.84 ± 0.034
4.224PheIle: 4.224 ± 0.085
3.849PheLys: 3.849 ± 0.073
4.987PheLeu: 4.987 ± 0.106
1.144PheMet: 1.144 ± 0.038
3.256PheAsn: 3.256 ± 0.068
1.743PhePro: 1.743 ± 0.047
1.634PheGln: 1.634 ± 0.047
1.605PheArg: 1.605 ± 0.046
4.198PheSer: 4.198 ± 0.084
3.167PheThr: 3.167 ± 0.068
3.357PheVal: 3.357 ± 0.082
0.553PheTrp: 0.553 ± 0.027
2.301PheTyr: 2.301 ± 0.057
0.0PheXaa: 0.0 ± 0.0
Gly
3.902GlyAla: 3.902 ± 0.088
0.667GlyCys: 0.667 ± 0.047
2.749GlyAsp: 2.749 ± 0.069
3.199GlyGlu: 3.199 ± 0.067
3.653GlyPhe: 3.653 ± 0.076
4.198GlyGly: 4.198 ± 0.106
1.058GlyHis: 1.058 ± 0.035
5.519GlyIle: 5.519 ± 0.095
5.145GlyLys: 5.145 ± 0.09
5.385GlyLeu: 5.385 ± 0.101
1.474GlyMet: 1.474 ± 0.043
3.745GlyAsn: 3.745 ± 0.091
1.125GlyPro: 1.125 ± 0.046
1.761GlyGln: 1.761 ± 0.058
1.792GlyArg: 1.792 ± 0.053
3.749GlySer: 3.749 ± 0.088
4.054GlyThr: 4.054 ± 0.16
3.777GlyVal: 3.777 ± 0.122
0.607GlyTrp: 0.607 ± 0.033
2.578GlyTyr: 2.578 ± 0.06
0.0GlyXaa: 0.0 ± 0.0
His
0.852HisAla: 0.852 ± 0.03
0.198HisCys: 0.198 ± 0.015
0.815HisAsp: 0.815 ± 0.035
0.96HisGlu: 0.96 ± 0.036
1.157HisPhe: 1.157 ± 0.047
0.888HisGly: 0.888 ± 0.039
0.44HisHis: 0.44 ± 0.029
1.417HisIle: 1.417 ± 0.048
1.26HisLys: 1.26 ± 0.043
1.711HisLeu: 1.711 ± 0.047
0.242HisMet: 0.242 ± 0.019
1.073HisAsn: 1.073 ± 0.037
0.67HisPro: 0.67 ± 0.029
0.737HisGln: 0.737 ± 0.029
0.523HisArg: 0.523 ± 0.027
1.016HisSer: 1.016 ± 0.039
0.846HisThr: 0.846 ± 0.032
0.762HisVal: 0.762 ± 0.03
0.17HisTrp: 0.17 ± 0.014
0.8HisTyr: 0.8 ± 0.033
0.0HisXaa: 0.0 ± 0.0
Ile
6.215IleAla: 6.215 ± 0.096
0.708IleCys: 0.708 ± 0.034
4.963IleAsp: 4.963 ± 0.082
6.068IleGlu: 6.068 ± 0.116
4.085IlePhe: 4.085 ± 0.074
5.305IleGly: 5.305 ± 0.094
1.412IleHis: 1.412 ± 0.048
7.66IleIle: 7.66 ± 0.126
6.927IleLys: 6.927 ± 0.115
7.765IleLeu: 7.765 ± 0.105
1.562IleMet: 1.562 ± 0.046
5.726IleAsn: 5.726 ± 0.089
3.56IlePro: 3.56 ± 0.068
2.934IleGln: 2.934 ± 0.069
2.591IleArg: 2.591 ± 0.05
6.2IleSer: 6.2 ± 0.095
5.412IleThr: 5.412 ± 0.108
5.082IleVal: 5.082 ± 0.084
0.705IleTrp: 0.705 ± 0.024
3.1IleTyr: 3.1 ± 0.06
0.0IleXaa: 0.0 ± 0.0
Lys
5.095LysAla: 5.095 ± 0.132
0.444LysCys: 0.444 ± 0.024
4.184LysAsp: 4.184 ± 0.089
6.258LysGlu: 6.258 ± 0.115
3.446LysPhe: 3.446 ± 0.074
4.463LysGly: 4.463 ± 0.079
1.363LysHis: 1.363 ± 0.048
8.223LysIle: 8.223 ± 0.119
8.067LysLys: 8.067 ± 0.142
7.336LysLeu: 7.336 ± 0.125
2.408LysMet: 2.408 ± 0.057
6.608LysAsn: 6.608 ± 0.1
2.734LysPro: 2.734 ± 0.069
3.028LysGln: 3.028 ± 0.07
2.611LysArg: 2.611 ± 0.058
4.918LysSer: 4.918 ± 0.082
5.534LysThr: 5.534 ± 0.098
4.731LysVal: 4.731 ± 0.091
0.81LysTrp: 0.81 ± 0.032
3.446LysTyr: 3.446 ± 0.071
0.0LysXaa: 0.0 ± 0.0
Leu
5.881LeuAla: 5.881 ± 0.096
0.706LeuCys: 0.706 ± 0.033
4.644LeuAsp: 4.644 ± 0.083
6.062LeuGlu: 6.062 ± 0.098
5.081LeuPhe: 5.081 ± 0.117
5.656LeuGly: 5.656 ± 0.093
1.515LeuHis: 1.515 ± 0.045
7.155LeuIle: 7.155 ± 0.12
7.961LeuLys: 7.961 ± 0.113
8.677LeuLeu: 8.677 ± 0.139
1.971LeuMet: 1.971 ± 0.049
5.981LeuAsn: 5.981 ± 0.117
3.396LeuPro: 3.396 ± 0.06
3.431LeuGln: 3.431 ± 0.075
2.741LeuArg: 2.741 ± 0.077
6.447LeuSer: 6.447 ± 0.079
4.958LeuThr: 4.958 ± 0.088
5.165LeuVal: 5.165 ± 0.084
0.68LeuTrp: 0.68 ± 0.03
3.052LeuTyr: 3.052 ± 0.071
0.0LeuXaa: 0.0 ± 0.0
Met
1.485MetAla: 1.485 ± 0.045
0.146MetCys: 0.146 ± 0.012
0.811MetAsp: 0.811 ± 0.032
1.116MetGlu: 1.116 ± 0.04
0.908MetPhe: 0.908 ± 0.04
1.229MetGly: 1.229 ± 0.037
0.386MetHis: 0.386 ± 0.022
1.692MetIle: 1.692 ± 0.051
2.3MetLys: 2.3 ± 0.051
1.866MetLeu: 1.866 ± 0.055
0.628MetMet: 0.628 ± 0.03
1.467MetAsn: 1.467 ± 0.046
0.799MetPro: 0.799 ± 0.034
0.932MetGln: 0.932 ± 0.036
0.794MetArg: 0.794 ± 0.032
1.481MetSer: 1.481 ± 0.041
1.257MetThr: 1.257 ± 0.038
1.373MetVal: 1.373 ± 0.044
0.16MetTrp: 0.16 ± 0.015
0.687MetTyr: 0.687 ± 0.03
0.0MetXaa: 0.0 ± 0.0
Asn
3.997AsnAla: 3.997 ± 0.078
0.62AsnCys: 0.62 ± 0.033
3.368AsnAsp: 3.368 ± 0.079
4.01AsnGlu: 4.01 ± 0.083
3.69AsnPhe: 3.69 ± 0.085
3.889AsnGly: 3.889 ± 0.09
1.285AsnHis: 1.285 ± 0.039
5.939AsnIle: 5.939 ± 0.09
5.573AsnLys: 5.573 ± 0.101
6.236AsnLeu: 6.236 ± 0.101
1.229AsnMet: 1.229 ± 0.037
4.691AsnAsn: 4.691 ± 0.111
3.021AsnPro: 3.021 ± 0.078
2.616AsnGln: 2.616 ± 0.061
2.007AsnArg: 2.007 ± 0.05
4.159AsnSer: 4.159 ± 0.099
4.231AsnThr: 4.231 ± 0.1
3.612AsnVal: 3.612 ± 0.087
0.75AsnTrp: 0.75 ± 0.033
3.341AsnTyr: 3.341 ± 0.088
0.0AsnXaa: 0.0 ± 0.0
Pro
1.935ProAla: 1.935 ± 0.078
0.225ProCys: 0.225 ± 0.018
1.537ProAsp: 1.537 ± 0.045
2.488ProGlu: 2.488 ± 0.056
1.924ProPhe: 1.924 ± 0.054
1.563ProGly: 1.563 ± 0.058
0.464ProHis: 0.464 ± 0.026
2.963ProIle: 2.963 ± 0.065
2.679ProLys: 2.679 ± 0.057
2.63ProLeu: 2.63 ± 0.059
0.626ProMet: 0.626 ± 0.028
2.509ProAsn: 2.509 ± 0.072
0.679ProPro: 0.679 ± 0.036
1.082ProGln: 1.082 ± 0.042
0.837ProArg: 0.837 ± 0.036
2.05ProSer: 2.05 ± 0.067
2.251ProThr: 2.251 ± 0.079
2.232ProVal: 2.232 ± 0.104
0.283ProTrp: 0.283 ± 0.021
1.243ProTyr: 1.243 ± 0.046
0.0ProXaa: 0.0 ± 0.0
Gln
2.079GlnAla: 2.079 ± 0.067
0.202GlnCys: 0.202 ± 0.016
1.665GlnAsp: 1.665 ± 0.045
2.544GlnGlu: 2.544 ± 0.061
1.781GlnPhe: 1.781 ± 0.05
1.743GlnGly: 1.743 ± 0.05
0.532GlnHis: 0.532 ± 0.026
3.145GlnIle: 3.145 ± 0.069
3.38GlnLys: 3.38 ± 0.078
3.337GlnLeu: 3.337 ± 0.067
0.825GlnMet: 0.825 ± 0.034
2.609GlnAsn: 2.609 ± 0.058
0.925GlnPro: 0.925 ± 0.031
1.324GlnGln: 1.324 ± 0.048
1.067GlnArg: 1.067 ± 0.036
1.991GlnSer: 1.991 ± 0.05
2.116GlnThr: 2.116 ± 0.059
1.917GlnVal: 1.917 ± 0.049
0.349GlnTrp: 0.349 ± 0.022
1.358GlnTyr: 1.358 ± 0.044
0.0GlnXaa: 0.0 ± 0.0
Arg
1.76ArgAla: 1.76 ± 0.052
0.198ArgCys: 0.198 ± 0.017
1.552ArgAsp: 1.552 ± 0.05
1.991ArgGlu: 1.991 ± 0.062
1.675ArgPhe: 1.675 ± 0.047
1.714ArgGly: 1.714 ± 0.054
0.533ArgHis: 0.533 ± 0.026
2.714ArgIle: 2.714 ± 0.065
2.712ArgLys: 2.712 ± 0.06
2.833ArgLeu: 2.833 ± 0.065
0.858ArgMet: 0.858 ± 0.035
1.99ArgAsn: 1.99 ± 0.049
0.938ArgPro: 0.938 ± 0.04
0.961ArgGln: 0.961 ± 0.037
1.078ArgArg: 1.078 ± 0.048
1.601ArgSer: 1.601 ± 0.042
1.657ArgThr: 1.657 ± 0.049
1.831ArgVal: 1.831 ± 0.056
0.279ArgTrp: 0.279 ± 0.019
1.237ArgTyr: 1.237 ± 0.039
0.0ArgXaa: 0.0 ± 0.0
Ser
3.664SerAla: 3.664 ± 0.08
0.651SerCys: 0.651 ± 0.03
3.395SerAsp: 3.395 ± 0.071
3.915SerGlu: 3.915 ± 0.076
4.009SerPhe: 4.009 ± 0.081
4.613SerGly: 4.613 ± 0.13
1.011SerHis: 1.011 ± 0.035
5.277SerIle: 5.277 ± 0.085
5.202SerLys: 5.202 ± 0.085
5.809SerLeu: 5.809 ± 0.087
1.197SerMet: 1.197 ± 0.037
4.157SerAsn: 4.157 ± 0.092
1.908SerPro: 1.908 ± 0.051
2.332SerGln: 2.332 ± 0.059
1.967SerArg: 1.967 ± 0.055
4.019SerSer: 4.019 ± 0.096
3.458SerThr: 3.458 ± 0.091
3.989SerVal: 3.989 ± 0.081
0.629SerTrp: 0.629 ± 0.034
2.614SerTyr: 2.614 ± 0.071
0.0SerXaa: 0.0 ± 0.0
Thr
3.95ThrAla: 3.95 ± 0.142
0.433ThrCys: 0.433 ± 0.039
2.892ThrAsp: 2.892 ± 0.065
3.411ThrGlu: 3.411 ± 0.075
3.13ThrPhe: 3.13 ± 0.07
3.94ThrGly: 3.94 ± 0.118
0.968ThrHis: 0.968 ± 0.035
6.14ThrIle: 6.14 ± 0.152
4.667ThrLys: 4.667 ± 0.091
5.305ThrLeu: 5.305 ± 0.102
0.924ThrMet: 0.924 ± 0.037
4.309ThrAsn: 4.309 ± 0.098
2.496ThrPro: 2.496 ± 0.079
2.104ThrGln: 2.104 ± 0.057
1.543ThrArg: 1.543 ± 0.042
3.881ThrSer: 3.881 ± 0.108
4.199ThrThr: 4.199 ± 0.155
3.796ThrVal: 3.796 ± 0.198
0.454ThrTrp: 0.454 ± 0.027
2.282ThrTyr: 2.282 ± 0.069
0.0ThrXaa: 0.0 ± 0.0
Val
4.132ValAla: 4.132 ± 0.125
0.551ValCys: 0.551 ± 0.031
2.906ValAsp: 2.906 ± 0.068
3.42ValGlu: 3.42 ± 0.076
3.369ValPhe: 3.369 ± 0.068
3.55ValGly: 3.55 ± 0.091
0.861ValHis: 0.861 ± 0.035
5.021ValIle: 5.021 ± 0.089
4.404ValLys: 4.404 ± 0.076
5.828ValLeu: 5.828 ± 0.089
1.254ValMet: 1.254 ± 0.037
3.687ValAsn: 3.687 ± 0.081
1.957ValPro: 1.957 ± 0.064
1.747ValGln: 1.747 ± 0.051
1.763ValArg: 1.763 ± 0.055
4.258ValSer: 4.258 ± 0.08
3.981ValThr: 3.981 ± 0.189
3.879ValVal: 3.879 ± 0.084
0.52ValTrp: 0.52 ± 0.033
2.171ValTyr: 2.171 ± 0.056
0.0ValXaa: 0.0 ± 0.0
Trp
0.537TrpAla: 0.537 ± 0.024
0.091TrpCys: 0.091 ± 0.009
0.445TrpAsp: 0.445 ± 0.025
0.569TrpGlu: 0.569 ± 0.028
0.572TrpPhe: 0.572 ± 0.032
0.49TrpGly: 0.49 ± 0.024
0.21TrpHis: 0.21 ± 0.02
0.716TrpIle: 0.716 ± 0.028
0.772TrpLys: 0.772 ± 0.036
0.856TrpLeu: 0.856 ± 0.036
0.236TrpMet: 0.236 ± 0.019
0.692TrpAsn: 0.692 ± 0.032
0.175TrpPro: 0.175 ± 0.028
0.409TrpGln: 0.409 ± 0.02
0.325TrpArg: 0.325 ± 0.023
0.576TrpSer: 0.576 ± 0.032
0.48TrpThr: 0.48 ± 0.027
0.532TrpVal: 0.532 ± 0.03
0.107TrpTrp: 0.107 ± 0.012
0.381TrpTyr: 0.381 ± 0.022
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.276TyrAla: 2.276 ± 0.047
0.363TyrCys: 0.363 ± 0.023
2.181TyrAsp: 2.181 ± 0.058
2.136TyrGlu: 2.136 ± 0.055
2.516TyrPhe: 2.516 ± 0.061
2.32TyrGly: 2.32 ± 0.064
0.773TyrHis: 0.773 ± 0.03
3.045TyrIle: 3.045 ± 0.064
3.378TyrLys: 3.378 ± 0.079
3.804TyrLeu: 3.804 ± 0.073
0.701TyrMet: 0.701 ± 0.031
2.842TyrAsn: 2.842 ± 0.069
1.391TyrPro: 1.391 ± 0.041
1.595TyrGln: 1.595 ± 0.044
1.284TyrArg: 1.284 ± 0.043
2.662TyrSer: 2.662 ± 0.058
2.362TyrThr: 2.362 ± 0.08
1.949TyrVal: 1.949 ± 0.047
0.396TyrTrp: 0.396 ± 0.021
1.874TyrTyr: 1.874 ± 0.059
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2421 proteins (806156 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski