Streptomyces sp. NRRL B-1568

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; unclassified Streptomyces

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2743 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0F4K262|A0A0F4K262_9ACTN Uncharacterized protein (Fragment) OS=Streptomyces sp. NRRL B-1568 OX=1609106 GN=VR41_14910 PE=4 SV=1
AA1 pKa = 7.42TLVFDD6 pKa = 4.34YY7 pKa = 7.48PTPGALADD15 pKa = 3.9YY16 pKa = 10.91LLDD19 pKa = 3.64EE20 pKa = 4.97LFGAEE25 pKa = 3.87AAVAVPAAGHH35 pKa = 5.89GVLAVADD42 pKa = 4.36DD43 pKa = 3.96PVVIVGMACRR53 pKa = 11.84YY54 pKa = 8.37PGGVGSPEE62 pKa = 4.02DD63 pKa = 3.32LWRR66 pKa = 11.84LVTEE70 pKa = 4.74GADD73 pKa = 3.16AA74 pKa = 4.81

Molecular weight: 7.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0F4K8X2|A0A0F4K8X2_9ACTN Family 2 glycosyl transferase OS=Streptomyces sp. NRRL B-1568 OX=1609106 GN=VR41_04620 PE=4 SV=1
PP1 pKa = 7.33GPPRR5 pKa = 11.84RR6 pKa = 11.84LWHH9 pKa = 6.65RR10 pKa = 11.84SRR12 pKa = 11.84PSRR15 pKa = 11.84RR16 pKa = 11.84PRR18 pKa = 11.84PSPSAPAPPRR28 pKa = 11.84RR29 pKa = 11.84PSRR32 pKa = 11.84PWTASKK38 pKa = 10.34PPRR41 pKa = 11.84PSRR44 pKa = 11.84SAPARR49 pKa = 11.84SRR51 pKa = 11.84RR52 pKa = 11.84LRR54 pKa = 11.84PPRR57 pKa = 11.84AEE59 pKa = 4.06VGVTAA64 pKa = 5.45

Molecular weight: 7.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2743

0

2743

879460

29

6558

320.6

34.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.892 ± 0.085

0.835 ± 0.013

5.855 ± 0.033

5.78 ± 0.047

2.773 ± 0.027

9.503 ± 0.043

2.424 ± 0.022

3.088 ± 0.036

2.142 ± 0.039

10.427 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.767 ± 0.02

1.707 ± 0.024

6.037 ± 0.037

2.809 ± 0.025

8.005 ± 0.055

4.991 ± 0.034

6.053 ± 0.042

8.433 ± 0.05

1.492 ± 0.018

1.988 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski