Amino acid dipepetide frequency for Streptomyces sp. NRRL B-1568

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	22.423AlaAla: 22.423 ± 0.292	1.263AlaCys: 1.263 ± 0.039	8.414AlaAsp: 8.414 ± 0.123	9.33AlaGlu: 9.33 ± 0.202	3.66AlaPhe: 3.66 ± 0.073	13.285AlaGly: 13.285 ± 0.138	3.088AlaHis: 3.088 ± 0.076	3.291AlaIle: 3.291 ± 0.075	2.92AlaLys: 2.92 ± 0.08	14.733AlaLeu: 14.733 ± 0.171	2.605AlaMet: 2.605 ± 0.051	1.835AlaAsn: 1.835 ± 0.043	7.394AlaPro: 7.394 ± 0.132	3.91AlaGln: 3.91 ± 0.066	10.415AlaArg: 10.415 ± 0.165	5.896AlaSer: 5.896 ± 0.087	6.933AlaThr: 6.933 ± 0.102	12.797AlaVal: 12.797 ± 0.15	1.931AlaTrp: 1.931 ± 0.053	2.797AlaTyr: 2.797 ± 0.063	0.0AlaXaa: 0.0 ± 0.0
Cys	1.112CysAla: 1.112 ± 0.039	0.089CysCys: 0.089 ± 0.011	0.516CysAsp: 0.516 ± 0.023	0.384CysGlu: 0.384 ± 0.02	0.244CysPhe: 0.244 ± 0.015	1.005CysGly: 1.005 ± 0.033	0.206CysHis: 0.206 ± 0.016	0.177CysIle: 0.177 ± 0.014	0.152CysLys: 0.152 ± 0.013	0.791CysLeu: 0.791 ± 0.037	0.126CysMet: 0.126 ± 0.012	0.128CysAsn: 0.128 ± 0.013	0.521CysPro: 0.521 ± 0.026	0.194CysGln: 0.194 ± 0.013	0.674CysArg: 0.674 ± 0.033	0.517CysSer: 0.517 ± 0.026	0.566CysThr: 0.566 ± 0.025	0.662CysVal: 0.662 ± 0.029	0.13CysTrp: 0.13 ± 0.012	0.15CysTyr: 0.15 ± 0.012	0.0CysXaa: 0.0 ± 0.0
Asp	7.944AspAla: 7.944 ± 0.105	0.428AspCys: 0.428 ± 0.023	3.485AspAsp: 3.485 ± 0.08	3.75AspGlu: 3.75 ± 0.072	1.66AspPhe: 1.66 ± 0.042	6.453AspGly: 6.453 ± 0.106	1.442AspHis: 1.442 ± 0.037	1.79AspIle: 1.79 ± 0.052	1.236AspLys: 1.236 ± 0.05	5.86AspLeu: 5.86 ± 0.084	0.77AspMet: 0.77 ± 0.033	0.943AspAsn: 0.943 ± 0.038	4.249AspPro: 4.249 ± 0.081	1.5AspGln: 1.5 ± 0.043	4.776AspArg: 4.776 ± 0.072	2.442AspSer: 2.442 ± 0.053	3.147AspThr: 3.147 ± 0.059	4.668AspVal: 4.668 ± 0.077	0.927AspTrp: 0.927 ± 0.029	1.078AspTyr: 1.078 ± 0.037	0.0AspXaa: 0.0 ± 0.0
Glu	8.007GluAla: 8.007 ± 0.2	0.439GluCys: 0.439 ± 0.021	2.834GluAsp: 2.834 ± 0.064	3.694GluGlu: 3.694 ± 0.094	1.485GluPhe: 1.485 ± 0.037	4.52GluGly: 4.52 ± 0.081	1.587GluHis: 1.587 ± 0.036	2.177GluIle: 2.177 ± 0.054	1.379GluLys: 1.379 ± 0.041	7.227GluLeu: 7.227 ± 0.116	0.954GluMet: 0.954 ± 0.038	0.953GluAsn: 0.953 ± 0.034	3.343GluPro: 3.343 ± 0.071	2.264GluGln: 2.264 ± 0.059	5.575GluArg: 5.575 ± 0.11	2.4GluSer: 2.4 ± 0.052	2.907GluThr: 2.907 ± 0.061	4.262GluVal: 4.262 ± 0.08	0.758GluTrp: 0.758 ± 0.027	1.03GluTyr: 1.03 ± 0.033	0.0GluXaa: 0.0 ± 0.0
Phe	3.907PheAla: 3.907 ± 0.066	0.257PheCys: 0.257 ± 0.017	2.004PheAsp: 2.004 ± 0.053	1.428PheGlu: 1.428 ± 0.043	0.938PhePhe: 0.938 ± 0.035	3.044PheGly: 3.044 ± 0.06	0.667PheHis: 0.667 ± 0.027	0.695PheIle: 0.695 ± 0.029	0.539PheLys: 0.539 ± 0.028	2.539PheLeu: 2.539 ± 0.058	0.372PheMet: 0.372 ± 0.02	0.55PheAsn: 0.55 ± 0.027	1.389PhePro: 1.389 ± 0.043	0.646PheGln: 0.646 ± 0.027	1.993PheArg: 1.993 ± 0.054	1.444PheSer: 1.444 ± 0.049	2.141PheThr: 2.141 ± 0.053	2.195PheVal: 2.195 ± 0.056	0.432PheTrp: 0.432 ± 0.023	0.548PheTyr: 0.548 ± 0.027	0.0PheXaa: 0.0 ± 0.0
Gly	11.041GlyAla: 11.041 ± 0.121	0.894GlyCys: 0.894 ± 0.035	4.954GlyAsp: 4.954 ± 0.081	5.381GlyGlu: 5.381 ± 0.079	2.93GlyPhe: 2.93 ± 0.069	8.813GlyGly: 8.813 ± 0.134	2.546GlyHis: 2.546 ± 0.059	3.607GlyIle: 3.607 ± 0.067	2.544GlyLys: 2.544 ± 0.075	9.579GlyLeu: 9.579 ± 0.108	2.112GlyMet: 2.112 ± 0.042	1.703GlyAsn: 1.703 ± 0.05	5.241GlyPro: 5.241 ± 0.078	2.748GlyGln: 2.748 ± 0.064	7.922GlyArg: 7.922 ± 0.102	5.23GlySer: 5.23 ± 0.093	6.432GlyThr: 6.432 ± 0.095	7.559GlyVal: 7.559 ± 0.113	1.695GlyTrp: 1.695 ± 0.047	2.102GlyTyr: 2.102 ± 0.054	0.0GlyXaa: 0.0 ± 0.0
His	2.854HisAla: 2.854 ± 0.067	0.23HisCys: 0.23 ± 0.018	1.408HisAsp: 1.408 ± 0.042	1.275HisGlu: 1.275 ± 0.038	0.716HisPhe: 0.716 ± 0.027	2.552HisGly: 2.552 ± 0.056	0.733HisHis: 0.733 ± 0.032	0.752HisIle: 0.752 ± 0.028	0.385HisLys: 0.385 ± 0.024	2.579HisLeu: 2.579 ± 0.062	0.37HisMet: 0.37 ± 0.02	0.39HisAsn: 0.39 ± 0.02	1.918HisPro: 1.918 ± 0.05	0.628HisGln: 0.628 ± 0.026	2.139HisArg: 2.139 ± 0.054	1.2HisSer: 1.2 ± 0.036	1.429HisThr: 1.429 ± 0.041	1.845HisVal: 1.845 ± 0.046	0.339HisTrp: 0.339 ± 0.02	0.5HisTyr: 0.5 ± 0.025	0.0HisXaa: 0.0 ± 0.0
Ile	4.759IleAla: 4.759 ± 0.074	0.271IleCys: 0.271 ± 0.016	2.175IleAsp: 2.175 ± 0.054	2.021IleGlu: 2.021 ± 0.055	0.673IlePhe: 0.673 ± 0.028	3.368IleGly: 3.368 ± 0.065	0.674IleHis: 0.674 ± 0.025	0.735IleIle: 0.735 ± 0.031	0.688IleLys: 0.688 ± 0.03	2.267IleLeu: 2.267 ± 0.059	0.416IleMet: 0.416 ± 0.023	0.644IleAsn: 0.644 ± 0.03	1.777IlePro: 1.777 ± 0.042	0.688IleGln: 0.688 ± 0.028	2.276IleArg: 2.276 ± 0.049	1.67IleSer: 1.67 ± 0.043	2.28IleThr: 2.28 ± 0.058	2.663IleVal: 2.663 ± 0.068	0.337IleTrp: 0.337 ± 0.019	0.497IleTyr: 0.497 ± 0.03	0.0IleXaa: 0.0 ± 0.0
Lys	2.976LysAla: 2.976 ± 0.072	0.139LysCys: 0.139 ± 0.013	1.288LysAsp: 1.288 ± 0.047	1.163LysGlu: 1.163 ± 0.045	0.462LysPhe: 0.462 ± 0.026	1.868LysGly: 1.868 ± 0.052	0.433LysHis: 0.433 ± 0.022	0.838LysIle: 0.838 ± 0.034	0.839LysLys: 0.839 ± 0.042	2.068LysLeu: 2.068 ± 0.06	0.393LysMet: 0.393 ± 0.024	0.515LysAsn: 0.515 ± 0.027	1.463LysPro: 1.463 ± 0.062	0.699LysGln: 0.699 ± 0.032	1.396LysArg: 1.396 ± 0.04	1.186LysSer: 1.186 ± 0.043	1.279LysThr: 1.279 ± 0.041	1.806LysVal: 1.806 ± 0.053	0.235LysTrp: 0.235 ± 0.018	0.373LysTyr: 0.373 ± 0.025	0.0LysXaa: 0.0 ± 0.0
Leu	15.774LeuAla: 15.774 ± 0.177	0.845LeuCys: 0.845 ± 0.034	6.59LeuAsp: 6.59 ± 0.095	4.946LeuGlu: 4.946 ± 0.094	2.63LeuPhe: 2.63 ± 0.058	9.405LeuGly: 9.405 ± 0.121	2.554LeuHis: 2.554 ± 0.063	3.111LeuIle: 3.111 ± 0.069	1.902LeuLys: 1.902 ± 0.053	11.294LeuLeu: 11.294 ± 0.151	1.646LeuMet: 1.646 ± 0.047	1.658LeuAsn: 1.658 ± 0.046	6.648LeuPro: 6.648 ± 0.093	2.23LeuGln: 2.23 ± 0.056	8.807LeuArg: 8.807 ± 0.124	5.35LeuSer: 5.35 ± 0.072	6.932LeuThr: 6.932 ± 0.093	8.855LeuVal: 8.855 ± 0.116	1.304LeuTrp: 1.304 ± 0.046	1.786LeuTyr: 1.786 ± 0.05	0.0LeuXaa: 0.0 ± 0.0
Met	2.386MetAla: 2.386 ± 0.057	0.152MetCys: 0.152 ± 0.013	0.919MetAsp: 0.919 ± 0.027	0.763MetGlu: 0.763 ± 0.032	0.467MetPhe: 0.467 ± 0.024	1.404MetGly: 1.404 ± 0.042	0.392MetHis: 0.392 ± 0.023	0.609MetIle: 0.609 ± 0.028	0.395MetLys: 0.395 ± 0.024	1.819MetLeu: 1.819 ± 0.046	0.322MetMet: 0.322 ± 0.019	0.407MetAsn: 0.407 ± 0.021	1.171MetPro: 1.171 ± 0.037	0.497MetGln: 0.497 ± 0.026	1.399MetArg: 1.399 ± 0.04	1.287MetSer: 1.287 ± 0.038	1.462MetThr: 1.462 ± 0.043	1.25MetVal: 1.25 ± 0.038	0.247MetTrp: 0.247 ± 0.019	0.321MetTyr: 0.321 ± 0.02	0.0MetXaa: 0.0 ± 0.0
Asn	2.158AsnAla: 2.158 ± 0.053	0.158AsnCys: 0.158 ± 0.015	0.865AsnAsp: 0.865 ± 0.032	0.791AsnGlu: 0.791 ± 0.033	0.474AsnPhe: 0.474 ± 0.023	1.934AsnGly: 1.934 ± 0.056	0.362AsnHis: 0.362 ± 0.023	0.605AsnIle: 0.605 ± 0.03	0.407AsnLys: 0.407 ± 0.023	1.657AsnLeu: 1.657 ± 0.041	0.263AsnMet: 0.263 ± 0.02	0.38AsnAsn: 0.38 ± 0.023	1.305AsnPro: 1.305 ± 0.041	0.492AsnGln: 0.492 ± 0.023	1.261AsnArg: 1.261 ± 0.039	0.932AsnSer: 0.932 ± 0.032	1.026AsnThr: 1.026 ± 0.036	1.351AsnVal: 1.351 ± 0.043	0.273AsnTrp: 0.273 ± 0.017	0.373AsnTyr: 0.373 ± 0.023	0.0AsnXaa: 0.0 ± 0.0
Pro	8.847ProAla: 8.847 ± 0.138	0.382ProCys: 0.382 ± 0.022	4.461ProAsp: 4.461 ± 0.069	4.249ProGlu: 4.249 ± 0.072	1.559ProPhe: 1.559 ± 0.048	6.722ProGly: 6.722 ± 0.115	1.43ProHis: 1.43 ± 0.049	1.218ProIle: 1.218 ± 0.04	1.225ProLys: 1.225 ± 0.046	5.437ProLeu: 5.437 ± 0.087	1.018ProMet: 1.018 ± 0.035	0.887ProAsn: 0.887 ± 0.03	3.322ProPro: 3.322 ± 0.087	1.944ProGln: 1.944 ± 0.054	3.894ProArg: 3.894 ± 0.09	3.039ProSer: 3.039 ± 0.065	3.022ProThr: 3.022 ± 0.074	5.52ProVal: 5.52 ± 0.089	0.932ProTrp: 0.932 ± 0.037	1.258ProTyr: 1.258 ± 0.041	0.0ProXaa: 0.0 ± 0.0
Gln	4.151GlnAla: 4.151 ± 0.068	0.184GlnCys: 0.184 ± 0.015	1.446GlnAsp: 1.446 ± 0.042	1.433GlnGlu: 1.433 ± 0.044	0.615GlnPhe: 0.615 ± 0.03	2.362GlnGly: 2.362 ± 0.053	0.731GlnHis: 0.731 ± 0.026	0.959GlnIle: 0.959 ± 0.037	0.581GlnLys: 0.581 ± 0.034	3.17GlnLeu: 3.17 ± 0.065	0.499GlnMet: 0.499 ± 0.024	0.462GlnAsn: 0.462 ± 0.026	1.725GlnPro: 1.725 ± 0.052	1.312GlnGln: 1.312 ± 0.047	2.491GlnArg: 2.491 ± 0.055	1.261GlnSer: 1.261 ± 0.038	1.382GlnThr: 1.382 ± 0.038	2.268GlnVal: 2.268 ± 0.05	0.486GlnTrp: 0.486 ± 0.02	0.569GlnTyr: 0.569 ± 0.026	0.0GlnXaa: 0.0 ± 0.0
Arg	9.722ArgAla: 9.722 ± 0.153	0.699ArgCys: 0.699 ± 0.027	4.056ArgAsp: 4.056 ± 0.078	4.935ArgGlu: 4.935 ± 0.098	2.313ArgPhe: 2.313 ± 0.061	5.781ArgGly: 5.781 ± 0.078	2.293ArgHis: 2.293 ± 0.054	3.376ArgIle: 3.376 ± 0.067	1.624ArgLys: 1.624 ± 0.045	9.164ArgLeu: 9.164 ± 0.138	1.744ArgMet: 1.744 ± 0.046	1.292ArgAsn: 1.292 ± 0.04	4.955ArgPro: 4.955 ± 0.085	2.394ArgGln: 2.394 ± 0.054	7.56ArgArg: 7.56 ± 0.121	3.957ArgSer: 3.957 ± 0.083	5.426ArgThr: 5.426 ± 0.089	5.678ArgVal: 5.678 ± 0.079	1.308ArgTrp: 1.308 ± 0.04	1.776ArgTyr: 1.776 ± 0.049	0.0ArgXaa: 0.0 ± 0.0
Ser	6.683SerAla: 6.683 ± 0.089	0.432SerCys: 0.432 ± 0.023	2.52SerAsp: 2.52 ± 0.06	2.321SerGlu: 2.321 ± 0.054	1.57SerPhe: 1.57 ± 0.045	5.904SerGly: 5.904 ± 0.107	1.059SerHis: 1.059 ± 0.036	1.367SerIle: 1.367 ± 0.039	0.996SerLys: 0.996 ± 0.031	5.011SerLeu: 5.011 ± 0.086	1.093SerMet: 1.093 ± 0.04	0.86SerAsn: 0.86 ± 0.032	3.088SerPro: 3.088 ± 0.067	1.18SerGln: 1.18 ± 0.039	3.635SerArg: 3.635 ± 0.067	2.793SerSer: 2.793 ± 0.067	2.936SerThr: 2.936 ± 0.061	4.382SerVal: 4.382 ± 0.07	0.912SerTrp: 0.912 ± 0.035	1.173SerTyr: 1.173 ± 0.036	0.0SerXaa: 0.0 ± 0.0
Thr	9.205ThrAla: 9.205 ± 0.117	0.397ThrCys: 0.397 ± 0.024	3.537ThrAsp: 3.537 ± 0.078	3.048ThrGlu: 3.048 ± 0.063	1.659ThrPhe: 1.659 ± 0.048	6.962ThrGly: 6.962 ± 0.095	1.205ThrHis: 1.205 ± 0.038	1.712ThrIle: 1.712 ± 0.046	1.06ThrLys: 1.06 ± 0.043	5.823ThrLeu: 5.823 ± 0.092	0.912ThrMet: 0.912 ± 0.031	1.067ThrAsn: 1.067 ± 0.038	3.861ThrPro: 3.861 ± 0.079	1.326ThrGln: 1.326 ± 0.039	3.616ThrArg: 3.616 ± 0.072	3.021ThrSer: 3.021 ± 0.062	3.715ThrThr: 3.715 ± 0.082	6.197ThrVal: 6.197 ± 0.101	0.88ThrTrp: 0.88 ± 0.033	1.33ThrTyr: 1.33 ± 0.046	0.0ThrXaa: 0.0 ± 0.0
Val	10.627ValAla: 10.627 ± 0.132	0.761ValCys: 0.761 ± 0.031	4.93ValAsp: 4.93 ± 0.08	4.809ValGlu: 4.809 ± 0.093	2.59ValPhe: 2.59 ± 0.058	6.336ValGly: 6.336 ± 0.103	2.055ValHis: 2.055 ± 0.049	2.95ValIle: 2.95 ± 0.066	1.752ValLys: 1.752 ± 0.05	9.5ValLeu: 9.5 ± 0.11	1.518ValMet: 1.518 ± 0.049	1.65ValAsn: 1.65 ± 0.045	5.183ValPro: 5.183 ± 0.081	2.163ValGln: 2.163 ± 0.051	7.168ValArg: 7.168 ± 0.101	4.472ValSer: 4.472 ± 0.068	5.454ValThr: 5.454 ± 0.093	7.774ValVal: 7.774 ± 0.124	1.155ValTrp: 1.155 ± 0.041	1.488ValTyr: 1.488 ± 0.041	0.0ValXaa: 0.0 ± 0.0
Trp	1.641TrpAla: 1.641 ± 0.046	0.151TrpCys: 0.151 ± 0.011	0.846TrpAsp: 0.846 ± 0.039	0.803TrpGlu: 0.803 ± 0.032	0.45TrpPhe: 0.45 ± 0.025	1.093TrpGly: 1.093 ± 0.04	0.379TrpHis: 0.379 ± 0.02	0.462TrpIle: 0.462 ± 0.021	0.391TrpLys: 0.391 ± 0.022	1.764TrpLeu: 1.764 ± 0.051	0.288TrpMet: 0.288 ± 0.019	0.371TrpAsn: 0.371 ± 0.023	0.791TrpPro: 0.791 ± 0.03	0.667TrpGln: 0.667 ± 0.029	1.272TrpArg: 1.272 ± 0.043	0.927TrpSer: 0.927 ± 0.036	1.004TrpThr: 1.004 ± 0.035	1.013TrpVal: 1.013 ± 0.033	0.301TrpTrp: 0.301 ± 0.016	0.302TrpTyr: 0.302 ± 0.019	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.705TyrAla: 2.705 ± 0.055	0.177TyrCys: 0.177 ± 0.014	1.297TyrAsp: 1.297 ± 0.042	1.274TyrGlu: 1.274 ± 0.043	0.631TyrPhe: 0.631 ± 0.029	2.224TyrGly: 2.224 ± 0.053	0.404TyrHis: 0.404 ± 0.022	0.449TyrIle: 0.449 ± 0.023	0.406TyrLys: 0.406 ± 0.026	1.985TyrLeu: 1.985 ± 0.048	0.249TyrMet: 0.249 ± 0.016	0.374TyrAsn: 0.374 ± 0.022	1.021TyrPro: 1.021 ± 0.039	0.603TyrGln: 0.603 ± 0.029	1.782TyrArg: 1.782 ± 0.049	0.888TyrSer: 0.888 ± 0.031	1.06TyrThr: 1.06 ± 0.037	1.59TyrVal: 1.59 ± 0.045	0.334TyrTrp: 0.334 ± 0.02	0.423TyrTyr: 0.423 ± 0.026	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2743 proteins (879460 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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