Canis familiaris papillomavirus 6
Average proteome isoelectric point is 6.16
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|C8YJL4|C8YJL4_9PAPI Replication protein E1 OS=Canis familiaris papillomavirus 6 OX=1513269 GN=E1 PE=3 SV=1
MM1 pKa = 7.61 IGQEE5 pKa = 4.02 PTLPDD10 pKa = 3.22 IVLTAQLPPEE20 pKa = 4.43 TVDD23 pKa = 2.94 LHH25 pKa = 8.24 CYY27 pKa = 7.4 EE28 pKa = 4.6 QIPAEE33 pKa = 4.12 EE34 pKa = 4.33 EE35 pKa = 4.18 EE36 pKa = 4.45 EE37 pKa = 4.2 EE38 pKa = 4.45 EE39 pKa = 4.08 PASRR43 pKa = 11.84 DD44 pKa = 3.55 LYY46 pKa = 10.78 RR47 pKa = 11.84 VAALCGLCGSRR58 pKa = 11.84 VRR60 pKa = 11.84 FVCLAEE66 pKa = 4.85 SDD68 pKa = 4.62 DD69 pKa = 3.35 IRR71 pKa = 11.84 HH72 pKa = 5.56 FQRR75 pKa = 11.84 LLFNLSFVCLRR86 pKa = 11.84 CVKK89 pKa = 9.57 TEE91 pKa = 3.85 KK92 pKa = 10.49 LNHH95 pKa = 6.27 GGG97 pKa = 3.44
Molecular weight: 10.97 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.611
IPC2_protein 4.774
IPC_protein 4.635
Toseland 4.52
ProMoST 4.724
Dawson 4.571
Bjellqvist 4.711
Wikipedia 4.418
Rodwell 4.495
Grimsley 4.431
Solomon 4.571
Lehninger 4.52
Nozaki 4.685
DTASelect 4.774
Thurlkill 4.507
EMBOSS 4.444
Sillero 4.749
Patrickios 3.3
IPC_peptide 4.584
IPC2_peptide 4.749
IPC2.peptide.svr19 4.696
Protein with the highest isoelectric point:
>tr|C8YJL6|C8YJL6_9PAPI E4 (Fragment) OS=Canis familiaris papillomavirus 6 OX=1513269 PE=4 SV=1
MM1 pKa = 7.63 EE2 pKa = 5.32 NLSKK6 pKa = 10.98 ALDD9 pKa = 3.73 SLQEE13 pKa = 4.1 EE14 pKa = 4.64 LLSLYY19 pKa = 9.66 EE20 pKa = 4.39 KK21 pKa = 10.91 NSGDD25 pKa = 3.11 IADD28 pKa = 5.35 QIRR31 pKa = 11.84 HH32 pKa = 4.87 WNLVRR37 pKa = 11.84 KK38 pKa = 8.51 EE39 pKa = 3.68 QVLFHH44 pKa = 6.48 FARR47 pKa = 11.84 KK48 pKa = 9.78 NGVSRR53 pKa = 11.84 IGMSPVPPQSVSQQKK68 pKa = 10.08 AKK70 pKa = 10.69 SAIEE74 pKa = 3.9 QEE76 pKa = 4.17 ILLQSLADD84 pKa = 3.56 SQYY87 pKa = 11.0 GSEE90 pKa = 4.28 RR91 pKa = 11.84 WTLSDD96 pKa = 3.23 TSKK99 pKa = 10.92 EE100 pKa = 3.9 RR101 pKa = 11.84 LLADD105 pKa = 3.91 PPYY108 pKa = 10.54 CFKK111 pKa = 11.33 KK112 pKa = 10.39 NGKK115 pKa = 8.86 QVDD118 pKa = 3.59 VRR120 pKa = 11.84 FDD122 pKa = 3.63 EE123 pKa = 4.71 QPDD126 pKa = 3.49 NLVRR130 pKa = 11.84 YY131 pKa = 7.48 VLWQYY136 pKa = 11.33 IYY138 pKa = 10.63 FQTEE142 pKa = 3.35 SDD144 pKa = 3.33 TWSKK148 pKa = 10.13 TEE150 pKa = 4.0 GKK152 pKa = 10.15 LDD154 pKa = 3.78 NLGLYY159 pKa = 10.27 YY160 pKa = 10.47 LDD162 pKa = 3.32 EE163 pKa = 4.41 HH164 pKa = 6.48 HH165 pKa = 7.05 FKK167 pKa = 10.86 VYY169 pKa = 10.68 YY170 pKa = 9.83 VDD172 pKa = 4.31 FRR174 pKa = 11.84 KK175 pKa = 9.92 EE176 pKa = 3.6 AEE178 pKa = 4.46 KK179 pKa = 10.73 YY180 pKa = 10.05 SKK182 pKa = 9.16 TGRR185 pKa = 11.84 YY186 pKa = 7.89 EE187 pKa = 4.04 VLSKK191 pKa = 10.25 LTTPAVPTSPFAATGPATGGDD212 pKa = 3.66 ASTSGATARR221 pKa = 11.84 PSTPKK226 pKa = 10.4 KK227 pKa = 9.82 KK228 pKa = 10.35 ALPTTPRR235 pKa = 11.84 RR236 pKa = 11.84 SRR238 pKa = 11.84 RR239 pKa = 11.84 YY240 pKa = 8.86 LRR242 pKa = 11.84 PSSRR246 pKa = 11.84 EE247 pKa = 3.57 TPRR250 pKa = 11.84 RR251 pKa = 11.84 RR252 pKa = 11.84 GFGRR256 pKa = 11.84 RR257 pKa = 11.84 RR258 pKa = 11.84 EE259 pKa = 4.23 GEE261 pKa = 4.12 LAGHH265 pKa = 5.87 STPSPGLVPPSPGEE279 pKa = 3.76 VGQRR283 pKa = 11.84 TQTTPKK289 pKa = 10.5 GNRR292 pKa = 11.84 TRR294 pKa = 11.84 LARR297 pKa = 11.84 LLLDD301 pKa = 4.38 AKK303 pKa = 10.24 DD304 pKa = 3.72 PPVIILKK311 pKa = 10.32 GGPNSLKK318 pKa = 10.35 CIRR321 pKa = 11.84 YY322 pKa = 8.33 RR323 pKa = 11.84 LKK325 pKa = 11.06 SKK327 pKa = 10.52 FSSLFSRR334 pKa = 11.84 ASTTWQWTVSDD345 pKa = 3.73 GTEE348 pKa = 3.14 RR349 pKa = 11.84 WGRR352 pKa = 11.84 SRR354 pKa = 11.84 MLLSFKK360 pKa = 10.07 DD361 pKa = 3.42 TAQRR365 pKa = 11.84 EE366 pKa = 4.62 LFLHH370 pKa = 6.0 TVQLPKK376 pKa = 10.37 SVQYY380 pKa = 11.24 SLGSFDD386 pKa = 5.74 DD387 pKa = 3.99 LL388 pKa = 5.16
Molecular weight: 44.05 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.118
IPC2_protein 9.238
IPC_protein 9.238
Toseland 9.94
ProMoST 9.633
Dawson 10.145
Bjellqvist 9.823
Wikipedia 10.321
Rodwell 10.526
Grimsley 10.218
Solomon 10.175
Lehninger 10.131
Nozaki 9.926
DTASelect 9.809
Thurlkill 9.999
EMBOSS 10.35
Sillero 10.058
Patrickios 9.897
IPC_peptide 10.175
IPC2_peptide 8.361
IPC2.peptide.svr19 8.225
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
2355
97
603
336.4
38.22
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.308 ± 0.304
1.953 ± 0.63
6.709 ± 0.548
6.709 ± 0.61
4.798 ± 0.504
5.605 ± 0.437
1.529 ± 0.131
4.756 ± 0.859
5.86 ± 0.764
9.894 ± 0.744
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.104 ± 0.267
3.355 ± 0.638
6.582 ± 1.231
4.798 ± 0.331
6.582 ± 0.838
7.856 ± 0.877
6.285 ± 0.787
5.478 ± 0.433
1.359 ± 0.337
3.482 ± 0.248
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here