Eubacterium aggregans

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae; Eubacterium

Average proteome isoelectric point is 5.89

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2753 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H4D0L4|A0A1H4D0L4_9FIRM Adenylosuccinate synthetase OS=Eubacterium aggregans OX=81409 GN=purA PE=3 SV=1
MM1 pKa = 7.7KK2 pKa = 10.22KK3 pKa = 10.16RR4 pKa = 11.84LGILGSLLLLAVLLAGCGSGGSGTLSADD32 pKa = 3.56DD33 pKa = 4.38VGTKK37 pKa = 10.22KK38 pKa = 9.25PTGEE42 pKa = 3.79KK43 pKa = 10.43SKK45 pKa = 10.17WAVYY49 pKa = 8.54WYY51 pKa = 10.66CCGSDD56 pKa = 3.66LEE58 pKa = 4.52SEE60 pKa = 4.45YY61 pKa = 11.4GAATADD67 pKa = 3.35IEE69 pKa = 4.43EE70 pKa = 4.43MLGATTSEE78 pKa = 4.13DD79 pKa = 3.35VKK81 pKa = 11.37VVVQTGGAAQWQNDD95 pKa = 3.56VVDD98 pKa = 3.87ASKK101 pKa = 10.63IEE103 pKa = 4.16RR104 pKa = 11.84YY105 pKa = 9.87EE106 pKa = 4.06FGSDD110 pKa = 3.15GLNLVDD116 pKa = 4.66QQPQADD122 pKa = 3.68MGEE125 pKa = 4.23AGTLQSFLSFCQKK138 pKa = 10.66NYY140 pKa = 9.81PADD143 pKa = 3.59NTMLLFWNHH152 pKa = 5.75GGGSVSGVSFDD163 pKa = 3.91EE164 pKa = 4.62NYY166 pKa = 10.83DD167 pKa = 3.76YY168 pKa = 11.66DD169 pKa = 4.36SLTLKK174 pKa = 10.64EE175 pKa = 4.68LDD177 pKa = 3.55TAIGSVYY184 pKa = 10.81SSGQKK189 pKa = 10.33LSVVGMDD196 pKa = 3.49TCLMATLDD204 pKa = 3.88NANIMKK210 pKa = 10.35KK211 pKa = 8.91YY212 pKa = 10.72ANYY215 pKa = 10.23LVASEE220 pKa = 4.33EE221 pKa = 4.23LEE223 pKa = 4.31PGNGWEE229 pKa = 4.07YY230 pKa = 10.83TKK232 pKa = 10.28WLSALGEE239 pKa = 4.32NPDD242 pKa = 3.52ISAVDD247 pKa = 3.97LGKK250 pKa = 10.52AICDD254 pKa = 3.49AFVEE258 pKa = 4.79GCEE261 pKa = 4.36DD262 pKa = 3.62AGTSDD267 pKa = 5.04EE268 pKa = 4.3ITLSVTDD275 pKa = 4.35LSKK278 pKa = 11.23VDD280 pKa = 3.97ALVSAVDD287 pKa = 3.53SMGTEE292 pKa = 4.38VLTKK296 pKa = 10.37AAGNSKK302 pKa = 10.25VVGQFGRR309 pKa = 11.84SARR312 pKa = 11.84AAEE315 pKa = 4.16NYY317 pKa = 9.88GGNNDD322 pKa = 3.42EE323 pKa = 5.34DD324 pKa = 5.93GYY326 pKa = 9.8TNMVDD331 pKa = 5.58LGDD334 pKa = 3.89LMKK337 pKa = 11.07NADD340 pKa = 4.47DD341 pKa = 6.2LIAATDD347 pKa = 3.87KK348 pKa = 11.27NVLSALEE355 pKa = 4.0SAVVYY360 pKa = 10.08KK361 pKa = 11.16VNGPYY366 pKa = 10.14RR367 pKa = 11.84SQASGLSTYY376 pKa = 10.59YY377 pKa = 10.75SYY379 pKa = 11.74DD380 pKa = 3.21GDD382 pKa = 3.82TDD384 pKa = 3.87SLAEE388 pKa = 4.05YY389 pKa = 9.65AQVAASPNYY398 pKa = 9.51TRR400 pKa = 11.84FIQYY404 pKa = 10.84SLGEE408 pKa = 4.22DD409 pKa = 3.36VSAEE413 pKa = 4.12VSQSTGVADD422 pKa = 3.69VQTVQGFNQQLNLSIDD438 pKa = 3.26GDD440 pKa = 4.23GYY442 pKa = 10.97INLKK446 pKa = 10.06LDD448 pKa = 3.75PAGLDD453 pKa = 3.23AVSSVTFDD461 pKa = 5.06LIYY464 pKa = 10.0MDD466 pKa = 3.96EE467 pKa = 4.65GVNEE471 pKa = 4.25MVFMGTDD478 pKa = 3.11NDD480 pKa = 4.84IDD482 pKa = 4.58ANWDD486 pKa = 3.3TGVFKK491 pKa = 11.2DD492 pKa = 3.76NFRR495 pKa = 11.84GVWGAMDD502 pKa = 4.27GNYY505 pKa = 10.55AYY507 pKa = 10.07MEE509 pKa = 3.98ITYY512 pKa = 10.65EE513 pKa = 4.12GDD515 pKa = 3.26DD516 pKa = 3.78YY517 pKa = 12.02NLYY520 pKa = 9.21TVPIKK525 pKa = 11.17LNGEE529 pKa = 4.23EE530 pKa = 4.27TNMTVSYY537 pKa = 10.62SFKK540 pKa = 10.61DD541 pKa = 3.42EE542 pKa = 3.97KK543 pKa = 11.01FKK545 pKa = 11.05ILGVSDD551 pKa = 5.68GIDD554 pKa = 3.59EE555 pKa = 4.32NTGMAEE561 pKa = 4.15RR562 pKa = 11.84SLKK565 pKa = 10.39ALKK568 pKa = 10.58KK569 pKa = 9.47GDD571 pKa = 4.05EE572 pKa = 4.68IILLSYY578 pKa = 10.36AMSMDD583 pKa = 4.1DD584 pKa = 5.89GNDD587 pKa = 2.96EE588 pKa = 4.25LYY590 pKa = 10.31EE591 pKa = 4.08YY592 pKa = 10.18EE593 pKa = 4.65SKK595 pKa = 10.46PIKK598 pKa = 10.58YY599 pKa = 10.14KK600 pKa = 10.95GDD602 pKa = 3.5TQFEE606 pKa = 4.45EE607 pKa = 4.89VEE609 pKa = 4.41LTNGDD614 pKa = 3.37YY615 pKa = 10.82AYY617 pKa = 9.78TFNVKK622 pKa = 8.76DD623 pKa = 3.71TTGKK627 pKa = 8.19TVSSDD632 pKa = 3.42YY633 pKa = 11.44AYY635 pKa = 10.04INYY638 pKa = 9.79NNGDD642 pKa = 3.31ITSSLEE648 pKa = 3.73

Molecular weight: 70.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H4CER7|A0A1H4CER7_9FIRM Diaminopropionate ammonia-lyase OS=Eubacterium aggregans OX=81409 GN=SAMN04515656_11520 PE=4 SV=1
MM1 pKa = 6.5TTFTIIFIYY10 pKa = 10.16CAAINLITFGAMGIDD25 pKa = 3.29KK26 pKa = 10.86AKK28 pKa = 10.37ARR30 pKa = 11.84RR31 pKa = 11.84GAWRR35 pKa = 11.84IPEE38 pKa = 4.07ATLFTLAIVGGSIGAIFGMTLFHH61 pKa = 7.19HH62 pKa = 5.96KK63 pKa = 8.97TKK65 pKa = 10.49HH66 pKa = 4.75LSFRR70 pKa = 11.84LGLPFILLVQLLGLYY85 pKa = 10.59LLLRR89 pKa = 11.84GG90 pKa = 4.13

Molecular weight: 9.92 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2753

0

2753

855004

29

2096

310.6

34.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.635 ± 0.057

1.478 ± 0.023

5.882 ± 0.04

6.721 ± 0.045

3.903 ± 0.033

7.95 ± 0.046

1.839 ± 0.022

7.439 ± 0.042

5.79 ± 0.038

9.186 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.081 ± 0.026

3.827 ± 0.033

3.828 ± 0.028

3.324 ± 0.025

4.375 ± 0.037

5.442 ± 0.036

5.838 ± 0.051

7.067 ± 0.039

0.859 ± 0.015

3.534 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski