Flavimobilis soli
Average proteome isoelectric point is 6.01
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2501 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2A9EC68|A0A2A9EC68_9MICO Serine hydroxymethyltransferase OS=Flavimobilis soli OX=442709 GN=glyA PE=3 SV=1
MM1 pKa = 7.17 TKK3 pKa = 10.22 SRR5 pKa = 11.84 LCRR8 pKa = 11.84 GVMAGSVLVLLLAGCSDD25 pKa = 4.5 DD26 pKa = 5.02 PEE28 pKa = 4.61 TPAPGTTGASSTTTDD43 pKa = 3.72 PAEE46 pKa = 4.54 TPQGPALCAEE56 pKa = 4.46 EE57 pKa = 4.69 TVADD61 pKa = 3.92 PASFATAGEE70 pKa = 4.15 IVAVQDD76 pKa = 3.91 EE77 pKa = 4.39 MSEE80 pKa = 4.08 AAGTSWRR87 pKa = 11.84 RR88 pKa = 11.84 YY89 pKa = 8.63 VPVTVTNPGTATCRR103 pKa = 11.84 VKK105 pKa = 11.13 AFVTVEE111 pKa = 4.02 GDD113 pKa = 3.01 GGAYY117 pKa = 9.61 EE118 pKa = 4.81 VDD120 pKa = 3.4 TADD123 pKa = 3.84 VVLEE127 pKa = 4.38 PGATANVQLFNLDD140 pKa = 3.66 DD141 pKa = 3.82 VVEE144 pKa = 4.3 FDD146 pKa = 4.4 GDD148 pKa = 4.0 TEE150 pKa = 4.4 GGQPVEE156 pKa = 4.11 QLTIAVQEE164 pKa = 4.27 VATWPVYY171 pKa = 10.48 DD172 pKa = 4.59 YY173 pKa = 11.69 YY174 pKa = 11.44 DD175 pKa = 4.34 ADD177 pKa = 3.9 VEE179 pKa = 4.67 FGAVSGEE186 pKa = 4.16 GADD189 pKa = 3.29 AVLPVTITKK198 pKa = 10.15 KK199 pKa = 10.19 AVRR202 pKa = 11.84 DD203 pKa = 4.04 GVPAPYY209 pKa = 10.82 GLTPQDD215 pKa = 3.21 DD216 pKa = 4.77 VYY218 pKa = 11.15 IQGVDD223 pKa = 2.97 ASGAVVAHH231 pKa = 6.25 FSAWALEE238 pKa = 4.32 AIEE241 pKa = 4.47 VGEE244 pKa = 4.28 TTTYY248 pKa = 10.03 EE249 pKa = 3.95 IPATIAARR257 pKa = 11.84 YY258 pKa = 9.54 DD259 pKa = 3.66 YY260 pKa = 10.84 EE261 pKa = 5.54 KK262 pKa = 11.07 DD263 pKa = 3.48 PEE265 pKa = 4.42 FPSYY269 pKa = 11.0 AYY271 pKa = 10.02 QGLSATEE278 pKa = 4.21 DD279 pKa = 3.36 VAEE282 pKa = 4.0 YY283 pKa = 10.13 RR284 pKa = 11.84 VVRR287 pKa = 11.84 FEE289 pKa = 4.03 PAFLEE294 pKa = 4.59 DD295 pKa = 3.37
Molecular weight: 31.03 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.719
IPC2_protein 3.757
IPC_protein 3.757
Toseland 3.554
ProMoST 3.872
Dawson 3.732
Bjellqvist 3.91
Wikipedia 3.643
Rodwell 3.579
Grimsley 3.452
Solomon 3.719
Lehninger 3.681
Nozaki 3.846
DTASelect 4.037
Thurlkill 3.592
EMBOSS 3.656
Sillero 3.872
Patrickios 0.896
IPC_peptide 3.719
IPC2_peptide 3.846
IPC2.peptide.svr19 3.783
Protein with the highest isoelectric point:
>tr|A0A2A9ED64|A0A2A9ED64_9MICO ThiF family protein OS=Flavimobilis soli OX=442709 GN=ATL41_0930 PE=4 SV=1
MM1 pKa = 7.4 GSVIKK6 pKa = 10.42 KK7 pKa = 8.47 RR8 pKa = 11.84 RR9 pKa = 11.84 KK10 pKa = 9.22 RR11 pKa = 11.84 MAKK14 pKa = 9.41 KK15 pKa = 9.87 KK16 pKa = 9.7 HH17 pKa = 5.84 RR18 pKa = 11.84 KK19 pKa = 8.56 LLRR22 pKa = 11.84 KK23 pKa = 7.78 TRR25 pKa = 11.84 HH26 pKa = 3.65 QRR28 pKa = 11.84 RR29 pKa = 11.84 NKK31 pKa = 9.78 KK32 pKa = 9.85
Molecular weight: 4.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.493
IPC2_protein 11.14
IPC_protein 12.691
Toseland 12.866
ProMoST 13.349
Dawson 12.866
Bjellqvist 12.852
Wikipedia 13.334
Rodwell 12.735
Grimsley 12.91
Solomon 13.349
Lehninger 13.261
Nozaki 12.866
DTASelect 12.852
Thurlkill 12.866
EMBOSS 13.364
Sillero 12.866
Patrickios 12.457
IPC_peptide 13.349
IPC2_peptide 12.34
IPC2.peptide.svr19 9.071
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2501
0
2501
860565
30
2427
344.1
36.56
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
14.058 ± 0.08
0.574 ± 0.012
6.311 ± 0.043
5.712 ± 0.048
2.596 ± 0.026
9.012 ± 0.04
1.99 ± 0.023
3.426 ± 0.032
2.076 ± 0.041
9.993 ± 0.061
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.714 ± 0.019
1.68 ± 0.021
5.637 ± 0.04
2.662 ± 0.023
7.491 ± 0.049
5.383 ± 0.036
6.458 ± 0.047
10.055 ± 0.046
1.367 ± 0.019
1.804 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here