Siphonobacter sp. BAB-5404

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Cytophagia; Cytophagales; Cytophagaceae; Siphonobacter; unclassified Siphonobacter

Average proteome isoelectric point is 6.8

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3865 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2N1KJ58|A0A2N1KJ58_9BACT Uncharacterized protein OS=Siphonobacter sp. BAB-5404 OX=1864824 GN=BWI96_18930 PE=4 SV=1
MM1 pKa = 7.58KK2 pKa = 10.4LFRR5 pKa = 11.84YY6 pKa = 7.97VTDD9 pKa = 3.42EE10 pKa = 4.0DD11 pKa = 4.73VIPGILIDD19 pKa = 4.0GKK21 pKa = 10.85SYY23 pKa = 10.66SLEE26 pKa = 4.06PFFEE30 pKa = 4.57ALGEE34 pKa = 4.08EE35 pKa = 4.15TGAYY39 pKa = 10.33DD40 pKa = 3.29FDD42 pKa = 4.87EE43 pKa = 4.61IFFEE47 pKa = 4.41EE48 pKa = 4.25EE49 pKa = 3.72GLAVLEE55 pKa = 5.48DD56 pKa = 3.89YY57 pKa = 11.1LDD59 pKa = 3.47QYY61 pKa = 9.62GTSEE65 pKa = 4.29LPEE68 pKa = 3.75LTGPIEE74 pKa = 4.15YY75 pKa = 9.57ASCVARR81 pKa = 11.84PSKK84 pKa = 10.33IICIGLNYY92 pKa = 9.74ADD94 pKa = 4.42HH95 pKa = 7.05AKK97 pKa = 8.13EE98 pKa = 4.06TNAPIPAEE106 pKa = 4.09PIVFFKK112 pKa = 10.61ATTALCGPNDD122 pKa = 3.9DD123 pKa = 5.17VIIPRR128 pKa = 11.84NSVKK132 pKa = 10.09TDD134 pKa = 3.12WEE136 pKa = 4.2VEE138 pKa = 4.05LAVVISSEE146 pKa = 3.76CRR148 pKa = 11.84YY149 pKa = 10.24VSEE152 pKa = 5.14EE153 pKa = 3.8EE154 pKa = 4.22AEE156 pKa = 4.4DD157 pKa = 4.19YY158 pKa = 10.69IAGYY162 pKa = 10.78AIMNDD167 pKa = 3.12YY168 pKa = 10.86SEE170 pKa = 6.31RR171 pKa = 11.84EE172 pKa = 3.94FQLEE176 pKa = 5.49RR177 pKa = 11.84GGQWDD182 pKa = 3.71KK183 pKa = 11.91GKK185 pKa = 10.98GCDD188 pKa = 3.39NFGPLGPYY196 pKa = 10.1LVTPEE201 pKa = 4.61EE202 pKa = 4.43IEE204 pKa = 4.78DD205 pKa = 3.81VTNLPMWLKK214 pKa = 11.16VNGEE218 pKa = 4.23TLQNSSTTQMIFNVPQIISYY238 pKa = 10.29LSQFMTLLPGDD249 pKa = 4.39VISTGTPPGVGLGFNPPRR267 pKa = 11.84YY268 pKa = 9.36LKK270 pKa = 10.96AGDD273 pKa = 3.51IVEE276 pKa = 4.63LGIEE280 pKa = 4.2GLGEE284 pKa = 3.82QRR286 pKa = 11.84QVAISWEE293 pKa = 3.89DD294 pKa = 3.27AYY296 pKa = 11.06

Molecular weight: 32.85 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2N1KKV3|A0A2N1KKV3_9BACT Por_Secre_tail domain-containing protein OS=Siphonobacter sp. BAB-5404 OX=1864824 GN=BWI96_15590 PE=4 SV=1
MM1 pKa = 7.75EE2 pKa = 5.16SFSVYY7 pKa = 10.21IPVILASMLKK17 pKa = 10.3FVGGPLAGLALNLTLTEE34 pKa = 4.73TILCTVGGMMLSVIIFTFLGRR55 pKa = 11.84IIRR58 pKa = 11.84FGWEE62 pKa = 4.16QIRR65 pKa = 11.84KK66 pKa = 6.55TPRR69 pKa = 11.84KK70 pKa = 9.62RR71 pKa = 11.84FTKK74 pKa = 9.51SSRR77 pKa = 11.84RR78 pKa = 11.84AVTIYY83 pKa = 10.54RR84 pKa = 11.84KK85 pKa = 10.08FGIAGIACLTPLLFTPIGGTLLAVSFKK112 pKa = 10.84ASPLRR117 pKa = 11.84IMSWMLVFAVFWAVIFCWAIYY138 pKa = 10.62NLADD142 pKa = 3.82LRR144 pKa = 11.84KK145 pKa = 9.92LILDD149 pKa = 3.92

Molecular weight: 16.69 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3865

0

3865

1279042

35

2416

330.9

37.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.446 ± 0.039

0.704 ± 0.012

4.939 ± 0.023

6.033 ± 0.039

4.791 ± 0.028

6.817 ± 0.039

1.919 ± 0.016

6.344 ± 0.029

5.978 ± 0.038

10.1 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.183 ± 0.016

4.824 ± 0.04

4.242 ± 0.024

4.427 ± 0.025

4.768 ± 0.025

6.551 ± 0.029

6.003 ± 0.03

6.522 ± 0.028

1.365 ± 0.015

4.044 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski