Amino acid dipepetide frequency for Siphonobacter sp. BAB-5404

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.987AlaAla: 5.987 ± 0.102
0.648AlaCys: 0.648 ± 0.022
3.9AlaAsp: 3.9 ± 0.064
4.828AlaGlu: 4.828 ± 0.069
3.586AlaPhe: 3.586 ± 0.053
5.481AlaGly: 5.481 ± 0.07
1.299AlaHis: 1.299 ± 0.034
4.76AlaIle: 4.76 ± 0.068
4.133AlaLys: 4.133 ± 0.071
7.653AlaLeu: 7.653 ± 0.087
1.655AlaMet: 1.655 ± 0.041
3.317AlaAsn: 3.317 ± 0.07
2.49AlaPro: 2.49 ± 0.048
3.471AlaGln: 3.471 ± 0.062
3.267AlaArg: 3.267 ± 0.061
5.012AlaSer: 5.012 ± 0.073
4.324AlaThr: 4.324 ± 0.069
4.702AlaVal: 4.702 ± 0.065
0.928AlaTrp: 0.928 ± 0.031
3.016AlaTyr: 3.016 ± 0.061
0.0AlaXaa: 0.0 ± 0.0
Cys
0.371CysAla: 0.371 ± 0.017
0.099CysCys: 0.099 ± 0.009
0.36CysAsp: 0.36 ± 0.019
0.382CysGlu: 0.382 ± 0.018
0.401CysPhe: 0.401 ± 0.018
0.536CysGly: 0.536 ± 0.023
0.2CysHis: 0.2 ± 0.013
0.458CysIle: 0.458 ± 0.02
0.342CysLys: 0.342 ± 0.017
0.79CysLeu: 0.79 ± 0.025
0.178CysMet: 0.178 ± 0.011
0.284CysAsn: 0.284 ± 0.014
0.312CysPro: 0.312 ± 0.016
0.335CysGln: 0.335 ± 0.017
0.327CysArg: 0.327 ± 0.014
0.477CysSer: 0.477 ± 0.022
0.395CysThr: 0.395 ± 0.02
0.428CysVal: 0.428 ± 0.018
0.109CysTrp: 0.109 ± 0.01
0.259CysTyr: 0.259 ± 0.015
0.0CysXaa: 0.0 ± 0.0
Asp
3.585AspAla: 3.585 ± 0.058
0.323AspCys: 0.323 ± 0.015
2.193AspAsp: 2.193 ± 0.046
3.173AspGlu: 3.173 ± 0.053
2.871AspPhe: 2.871 ± 0.043
3.501AspGly: 3.501 ± 0.052
1.006AspHis: 1.006 ± 0.027
2.98AspIle: 2.98 ± 0.054
2.622AspLys: 2.622 ± 0.052
5.23AspLeu: 5.23 ± 0.068
0.949AspMet: 0.949 ± 0.033
2.057AspAsn: 2.057 ± 0.04
2.134AspPro: 2.134 ± 0.041
2.201AspGln: 2.201 ± 0.042
2.614AspArg: 2.614 ± 0.047
2.926AspSer: 2.926 ± 0.056
2.549AspThr: 2.549 ± 0.045
3.208AspVal: 3.208 ± 0.059
0.897AspTrp: 0.897 ± 0.023
2.374AspTyr: 2.374 ± 0.047
0.0AspXaa: 0.0 ± 0.0
Glu
5.097GluAla: 5.097 ± 0.089
0.299GluCys: 0.299 ± 0.015
2.742GluAsp: 2.742 ± 0.053
4.299GluGlu: 4.299 ± 0.08
2.506GluPhe: 2.506 ± 0.05
3.7GluGly: 3.7 ± 0.06
1.163GluHis: 1.163 ± 0.035
3.917GluIle: 3.917 ± 0.067
4.691GluLys: 4.691 ± 0.062
6.091GluLeu: 6.091 ± 0.085
1.511GluMet: 1.511 ± 0.037
3.049GluAsn: 3.049 ± 0.046
1.969GluPro: 1.969 ± 0.039
2.586GluGln: 2.586 ± 0.05
3.018GluArg: 3.018 ± 0.053
3.238GluSer: 3.238 ± 0.055
3.251GluThr: 3.251 ± 0.051
4.267GluVal: 4.267 ± 0.063
0.78GluTrp: 0.78 ± 0.024
2.162GluTyr: 2.162 ± 0.043
0.0GluXaa: 0.0 ± 0.0
Phe
3.211PheAla: 3.211 ± 0.056
0.442PheCys: 0.442 ± 0.019
2.594PheAsp: 2.594 ± 0.046
2.745PheGlu: 2.745 ± 0.048
2.432PhePhe: 2.432 ± 0.053
3.307PheGly: 3.307 ± 0.05
0.934PheHis: 0.934 ± 0.026
2.975PheIle: 2.975 ± 0.054
2.503PheLys: 2.503 ± 0.047
4.804PheLeu: 4.804 ± 0.07
1.093PheMet: 1.093 ± 0.031
2.356PheAsn: 2.356 ± 0.047
1.898PhePro: 1.898 ± 0.042
1.992PheGln: 1.992 ± 0.046
2.316PheArg: 2.316 ± 0.045
3.605PheSer: 3.605 ± 0.056
3.03PheThr: 3.03 ± 0.053
2.946PheVal: 2.946 ± 0.049
0.762PheTrp: 0.762 ± 0.026
1.967PheTyr: 1.967 ± 0.04
0.0PheXaa: 0.0 ± 0.0
Gly
4.284GlyAla: 4.284 ± 0.076
0.57GlyCys: 0.57 ± 0.021
3.08GlyAsp: 3.08 ± 0.059
3.546GlyGlu: 3.546 ± 0.062
3.524GlyPhe: 3.524 ± 0.047
4.904GlyGly: 4.904 ± 0.084
1.22GlyHis: 1.22 ± 0.034
4.911GlyIle: 4.911 ± 0.061
4.8GlyLys: 4.8 ± 0.068
6.938GlyLeu: 6.938 ± 0.078
1.711GlyMet: 1.711 ± 0.036
3.35GlyAsn: 3.35 ± 0.063
1.777GlyPro: 1.777 ± 0.037
2.712GlyGln: 2.712 ± 0.049
3.032GlyArg: 3.032 ± 0.055
4.594GlySer: 4.594 ± 0.077
4.327GlyThr: 4.327 ± 0.074
4.729GlyVal: 4.729 ± 0.066
1.109GlyTrp: 1.109 ± 0.032
3.054GlyTyr: 3.054 ± 0.048
0.0GlyXaa: 0.0 ± 0.0
His
1.22HisAla: 1.22 ± 0.034
0.179HisCys: 0.179 ± 0.012
0.801HisAsp: 0.801 ± 0.028
1.16HisGlu: 1.16 ± 0.031
1.117HisPhe: 1.117 ± 0.032
1.131HisGly: 1.131 ± 0.032
0.535HisHis: 0.535 ± 0.022
1.113HisIle: 1.113 ± 0.03
0.858HisLys: 0.858 ± 0.029
2.149HisLeu: 2.149 ± 0.043
0.324HisMet: 0.324 ± 0.019
0.723HisAsn: 0.723 ± 0.027
1.106HisPro: 1.106 ± 0.032
0.999HisGln: 0.999 ± 0.027
0.985HisArg: 0.985 ± 0.027
1.213HisSer: 1.213 ± 0.035
1.15HisThr: 1.15 ± 0.034
1.074HisVal: 1.074 ± 0.033
0.365HisTrp: 0.365 ± 0.018
0.987HisTyr: 0.987 ± 0.028
0.0HisXaa: 0.0 ± 0.0
Ile
4.827IleAla: 4.827 ± 0.067
0.572IleCys: 0.572 ± 0.021
3.416IleAsp: 3.416 ± 0.054
3.871IleGlu: 3.871 ± 0.062
2.723IlePhe: 2.723 ± 0.058
4.451IleGly: 4.451 ± 0.062
1.379IleHis: 1.379 ± 0.033
3.857IleIle: 3.857 ± 0.065
3.274IleLys: 3.274 ± 0.053
5.93IleLeu: 5.93 ± 0.074
1.064IleMet: 1.064 ± 0.027
3.09IleAsn: 3.09 ± 0.054
3.342IlePro: 3.342 ± 0.05
2.858IleGln: 2.858 ± 0.05
3.474IleArg: 3.474 ± 0.052
4.391IleSer: 4.391 ± 0.068
4.125IleThr: 4.125 ± 0.068
3.777IleVal: 3.777 ± 0.064
0.758IleTrp: 0.758 ± 0.025
2.262IleTyr: 2.262 ± 0.047
0.0IleXaa: 0.0 ± 0.0
Lys
5.094LysAla: 5.094 ± 0.076
0.242LysCys: 0.242 ± 0.016
3.245LysAsp: 3.245 ± 0.066
4.011LysGlu: 4.011 ± 0.066
2.061LysPhe: 2.061 ± 0.047
4.131LysGly: 4.131 ± 0.062
1.052LysHis: 1.052 ± 0.03
3.811LysIle: 3.811 ± 0.06
4.162LysLys: 4.162 ± 0.074
5.219LysLeu: 5.219 ± 0.076
1.544LysMet: 1.544 ± 0.033
3.148LysAsn: 3.148 ± 0.055
2.82LysPro: 2.82 ± 0.055
2.356LysGln: 2.356 ± 0.049
2.71LysArg: 2.71 ± 0.044
3.599LysSer: 3.599 ± 0.052
3.64LysThr: 3.64 ± 0.058
4.046LysVal: 4.046 ± 0.062
0.688LysTrp: 0.688 ± 0.022
2.206LysTyr: 2.206 ± 0.047
0.0LysXaa: 0.0 ± 0.0
Leu
7.725LeuAla: 7.725 ± 0.093
0.733LeuCys: 0.733 ± 0.024
4.8LeuAsp: 4.8 ± 0.059
5.768LeuGlu: 5.768 ± 0.076
4.783LeuPhe: 4.783 ± 0.067
6.522LeuGly: 6.522 ± 0.096
1.857LeuHis: 1.857 ± 0.037
6.335LeuIle: 6.335 ± 0.083
6.519LeuLys: 6.519 ± 0.082
10.463LeuLeu: 10.463 ± 0.125
2.247LeuMet: 2.247 ± 0.047
4.973LeuAsn: 4.973 ± 0.071
4.695LeuPro: 4.695 ± 0.061
4.127LeuGln: 4.127 ± 0.065
4.757LeuArg: 4.757 ± 0.066
7.376LeuSer: 7.376 ± 0.08
6.366LeuThr: 6.366 ± 0.065
6.331LeuVal: 6.331 ± 0.092
1.179LeuTrp: 1.179 ± 0.038
3.439LeuTyr: 3.439 ± 0.05
0.0LeuXaa: 0.0 ± 0.0
Met
1.912MetAla: 1.912 ± 0.041
0.109MetCys: 0.109 ± 0.01
1.105MetAsp: 1.105 ± 0.029
1.234MetGlu: 1.234 ± 0.029
0.705MetPhe: 0.705 ± 0.023
1.576MetGly: 1.576 ± 0.039
0.431MetHis: 0.431 ± 0.019
1.385MetIle: 1.385 ± 0.033
1.866MetLys: 1.866 ± 0.038
2.027MetLeu: 2.027 ± 0.045
0.557MetMet: 0.557 ± 0.02
1.301MetAsn: 1.301 ± 0.03
1.089MetPro: 1.089 ± 0.026
0.912MetGln: 0.912 ± 0.029
0.98MetArg: 0.98 ± 0.03
1.298MetSer: 1.298 ± 0.037
1.196MetThr: 1.196 ± 0.031
1.349MetVal: 1.349 ± 0.038
0.18MetTrp: 0.18 ± 0.012
0.622MetTyr: 0.622 ± 0.023
0.0MetXaa: 0.0 ± 0.0
Asn
3.668AsnAla: 3.668 ± 0.055
0.297AsnCys: 0.297 ± 0.017
2.247AsnAsp: 2.247 ± 0.042
2.653AsnGlu: 2.653 ± 0.048
2.322AsnPhe: 2.322 ± 0.042
3.459AsnGly: 3.459 ± 0.068
0.96AsnHis: 0.96 ± 0.029
2.662AsnIle: 2.662 ± 0.054
2.314AsnLys: 2.314 ± 0.046
4.689AsnLeu: 4.689 ± 0.069
0.906AsnMet: 0.906 ± 0.026
2.288AsnAsn: 2.288 ± 0.066
2.905AsnPro: 2.905 ± 0.048
2.457AsnGln: 2.457 ± 0.05
2.782AsnArg: 2.782 ± 0.059
2.805AsnSer: 2.805 ± 0.056
2.803AsnThr: 2.803 ± 0.055
3.006AsnVal: 3.006 ± 0.05
0.74AsnTrp: 0.74 ± 0.026
2.275AsnTyr: 2.275 ± 0.046
0.0AsnXaa: 0.0 ± 0.0
Pro
3.441ProAla: 3.441 ± 0.069
0.238ProCys: 0.238 ± 0.014
2.546ProAsp: 2.546 ± 0.046
3.341ProGlu: 3.341 ± 0.053
2.098ProPhe: 2.098 ± 0.043
2.826ProGly: 2.826 ± 0.048
0.791ProHis: 0.791 ± 0.03
2.57ProIle: 2.57 ± 0.047
2.263ProLys: 2.263 ± 0.044
4.03ProLeu: 4.03 ± 0.063
0.865ProMet: 0.865 ± 0.026
2.098ProAsn: 2.098 ± 0.042
1.242ProPro: 1.242 ± 0.041
1.74ProGln: 1.74 ± 0.04
1.528ProArg: 1.528 ± 0.038
2.82ProSer: 2.82 ± 0.051
2.544ProThr: 2.544 ± 0.04
3.207ProVal: 3.207 ± 0.053
0.498ProTrp: 0.498 ± 0.02
1.733ProTyr: 1.733 ± 0.035
0.0ProXaa: 0.0 ± 0.0
Gln
3.522GlnAla: 3.522 ± 0.057
0.177GlnCys: 0.177 ± 0.013
1.834GlnAsp: 1.834 ± 0.037
2.733GlnGlu: 2.733 ± 0.038
1.865GlnPhe: 1.865 ± 0.041
2.556GlnGly: 2.556 ± 0.054
0.867GlnHis: 0.867 ± 0.027
2.739GlnIle: 2.739 ± 0.044
2.879GlnLys: 2.879 ± 0.052
4.512GlnLeu: 4.512 ± 0.067
0.998GlnMet: 0.998 ± 0.028
2.111GlnAsn: 2.111 ± 0.044
1.953GlnPro: 1.953 ± 0.044
2.526GlnGln: 2.526 ± 0.054
2.134GlnArg: 2.134 ± 0.044
2.764GlnSer: 2.764 ± 0.048
2.722GlnThr: 2.722 ± 0.049
3.108GlnVal: 3.108 ± 0.047
0.605GlnTrp: 0.605 ± 0.024
1.665GlnTyr: 1.665 ± 0.036
0.0GlnXaa: 0.0 ± 0.0
Arg
2.797ArgAla: 2.797 ± 0.051
0.272ArgCys: 0.272 ± 0.015
2.123ArgAsp: 2.123 ± 0.046
2.841ArgGlu: 2.841 ± 0.051
2.481ArgPhe: 2.481 ± 0.043
2.628ArgGly: 2.628 ± 0.05
0.845ArgHis: 0.845 ± 0.027
3.482ArgIle: 3.482 ± 0.054
3.212ArgLys: 3.212 ± 0.053
4.987ArgLeu: 4.987 ± 0.061
1.303ArgMet: 1.303 ± 0.031
2.481ArgAsn: 2.481 ± 0.048
1.887ArgPro: 1.887 ± 0.039
2.381ArgGln: 2.381 ± 0.046
2.367ArgArg: 2.367 ± 0.046
2.946ArgSer: 2.946 ± 0.05
2.719ArgThr: 2.719 ± 0.048
2.998ArgVal: 2.998 ± 0.058
0.762ArgTrp: 0.762 ± 0.027
2.163ArgTyr: 2.163 ± 0.04
0.0ArgXaa: 0.0 ± 0.0
Ser
4.337SerAla: 4.337 ± 0.069
0.539SerCys: 0.539 ± 0.021
3.184SerAsp: 3.184 ± 0.052
3.533SerGlu: 3.533 ± 0.055
3.535SerPhe: 3.535 ± 0.055
4.923SerGly: 4.923 ± 0.075
1.217SerHis: 1.217 ± 0.028
4.329SerIle: 4.329 ± 0.064
3.496SerLys: 3.496 ± 0.052
7.044SerLeu: 7.044 ± 0.078
1.345SerMet: 1.345 ± 0.031
2.973SerAsn: 2.973 ± 0.062
2.723SerPro: 2.723 ± 0.047
2.703SerGln: 2.703 ± 0.048
3.012SerArg: 3.012 ± 0.048
4.517SerSer: 4.517 ± 0.086
3.89SerThr: 3.89 ± 0.055
4.391SerVal: 4.391 ± 0.061
0.954SerTrp: 0.954 ± 0.03
2.865SerTyr: 2.865 ± 0.053
0.0SerXaa: 0.0 ± 0.0
Thr
4.525ThrAla: 4.525 ± 0.06
0.357ThrCys: 0.357 ± 0.018
3.198ThrAsp: 3.198 ± 0.047
3.259ThrGlu: 3.259 ± 0.058
3.007ThrPhe: 3.007 ± 0.046
4.491ThrGly: 4.491 ± 0.058
1.147ThrHis: 1.147 ± 0.029
3.913ThrIle: 3.913 ± 0.066
3.001ThrLys: 3.001 ± 0.06
6.405ThrLeu: 6.405 ± 0.072
0.984ThrMet: 0.984 ± 0.028
2.762ThrAsn: 2.762 ± 0.054
3.156ThrPro: 3.156 ± 0.058
2.616ThrGln: 2.616 ± 0.048
2.43ThrArg: 2.43 ± 0.048
3.904ThrSer: 3.904 ± 0.062
3.59ThrThr: 3.59 ± 0.067
3.94ThrVal: 3.94 ± 0.056
0.705ThrTrp: 0.705 ± 0.023
2.636ThrTyr: 2.636 ± 0.056
0.0ThrXaa: 0.0 ± 0.0
Val
4.929ValAla: 4.929 ± 0.068
0.545ValCys: 0.545 ± 0.021
3.244ValAsp: 3.244 ± 0.055
3.833ValGlu: 3.833 ± 0.067
3.106ValPhe: 3.106 ± 0.05
4.312ValGly: 4.312 ± 0.064
1.149ValHis: 1.149 ± 0.031
4.392ValIle: 4.392 ± 0.067
3.867ValLys: 3.867 ± 0.055
6.384ValLeu: 6.384 ± 0.083
1.499ValMet: 1.499 ± 0.037
3.199ValAsn: 3.199 ± 0.057
2.698ValPro: 2.698 ± 0.045
2.5ValGln: 2.5 ± 0.045
3.073ValArg: 3.073 ± 0.047
4.61ValSer: 4.61 ± 0.057
4.228ValThr: 4.228 ± 0.065
4.447ValVal: 4.447 ± 0.068
0.82ValTrp: 0.82 ± 0.026
2.382ValTyr: 2.382 ± 0.046
0.0ValXaa: 0.0 ± 0.0
Trp
1.014TrpAla: 1.014 ± 0.028
0.106TrpCys: 0.106 ± 0.009
0.654TrpAsp: 0.654 ± 0.024
0.775TrpGlu: 0.775 ± 0.021
0.627TrpPhe: 0.627 ± 0.025
0.969TrpGly: 0.969 ± 0.024
0.27TrpHis: 0.27 ± 0.015
0.895TrpIle: 0.895 ± 0.028
0.942TrpLys: 0.942 ± 0.023
1.456TrpLeu: 1.456 ± 0.035
0.401TrpMet: 0.401 ± 0.02
0.741TrpAsn: 0.741 ± 0.027
0.418TrpPro: 0.418 ± 0.02
0.659TrpGln: 0.659 ± 0.024
0.6TrpArg: 0.6 ± 0.022
0.784TrpSer: 0.784 ± 0.026
0.693TrpThr: 0.693 ± 0.025
0.914TrpVal: 0.914 ± 0.029
0.224TrpTrp: 0.224 ± 0.012
0.509TrpTyr: 0.509 ± 0.021
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.914TyrAla: 2.914 ± 0.052
0.296TyrCys: 0.296 ± 0.014
2.128TyrAsp: 2.128 ± 0.053
2.35TyrGlu: 2.35 ± 0.043
2.163TyrPhe: 2.163 ± 0.048
2.766TyrGly: 2.766 ± 0.049
0.869TyrHis: 0.869 ± 0.026
1.958TyrIle: 1.958 ± 0.041
2.037TyrLys: 2.037 ± 0.039
4.193TyrLeu: 4.193 ± 0.063
0.697TyrMet: 0.697 ± 0.023
1.937TyrAsn: 1.937 ± 0.047
1.807TyrPro: 1.807 ± 0.039
2.141TyrGln: 2.141 ± 0.047
2.267TyrArg: 2.267 ± 0.038
2.632TyrSer: 2.632 ± 0.046
2.483TyrThr: 2.483 ± 0.049
2.351TyrVal: 2.351 ± 0.041
0.587TyrTrp: 0.587 ± 0.02
1.861TyrTyr: 1.861 ± 0.046
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3865 proteins (1279042 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski