Sinobacterium caligoides

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Cellvibrionales; Spongiibacteraceae; Sinobacterium

Average proteome isoelectric point is 6.07

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3807 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3N2DKC4|A0A3N2DKC4_9GAMM Hemoglobin OS=Sinobacterium caligoides OX=933926 GN=EDC56_2891 PE=4 SV=1
MM1 pKa = 7.63KK2 pKa = 9.91KK3 pKa = 10.59LLMATMISLAASAQAEE19 pKa = 4.46IITFDD24 pKa = 3.61MQVLDD29 pKa = 4.74VITQGGEE36 pKa = 3.83AISANEE42 pKa = 3.68GAIFAEE48 pKa = 4.48LDD50 pKa = 2.86IDD52 pKa = 3.82TDD54 pKa = 3.34IGEE57 pKa = 4.69GEE59 pKa = 4.17LFGINSFSMTYY70 pKa = 10.27VEE72 pKa = 4.77ALTVEE77 pKa = 4.68EE78 pKa = 4.75SFQGDD83 pKa = 3.87EE84 pKa = 4.18QVHH87 pKa = 5.72IAHH90 pKa = 6.99TYY92 pKa = 7.6EE93 pKa = 3.53QGSRR97 pKa = 11.84YY98 pKa = 7.79TFNTTSFDD106 pKa = 3.57IEE108 pKa = 4.3DD109 pKa = 3.9RR110 pKa = 11.84DD111 pKa = 3.76EE112 pKa = 5.71GEE114 pKa = 4.42LDD116 pKa = 3.34GFAYY120 pKa = 10.66LAFTGGADD128 pKa = 3.46SGEE131 pKa = 4.05MSSFDD136 pKa = 4.08GGYY139 pKa = 9.9NCQSSSAAVEE149 pKa = 4.22GCEE152 pKa = 4.25GVSGTAIDD160 pKa = 4.2AYY162 pKa = 10.67GLVLLVHH169 pKa = 6.4WVEE172 pKa = 4.33SEE174 pKa = 3.83PTILDD179 pKa = 3.39FLVFTDD185 pKa = 3.37ITTGGAYY192 pKa = 9.44SEE194 pKa = 4.57KK195 pKa = 10.81LSFFYY200 pKa = 9.58GTGAFVAWGSAQPISDD216 pKa = 3.53IADD219 pKa = 3.48EE220 pKa = 4.84LEE222 pKa = 4.53PTTSPSPSEE231 pKa = 4.12PSSQGIVVDD240 pKa = 5.45CDD242 pKa = 3.79INWSQMCEE250 pKa = 4.0NLPVSQVPVPGAVWLFSSALAGFGILARR278 pKa = 11.84RR279 pKa = 11.84RR280 pKa = 11.84NRR282 pKa = 11.84IASS285 pKa = 3.52

Molecular weight: 30.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3N2DHF6|A0A3N2DHF6_9GAMM Histidine kinase OS=Sinobacterium caligoides OX=933926 GN=EDC56_3288 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.49RR12 pKa = 11.84KK13 pKa = 7.87RR14 pKa = 11.84THH16 pKa = 5.99GFRR19 pKa = 11.84SRR21 pKa = 11.84MATKK25 pKa = 10.35SGRR28 pKa = 11.84ALINRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.64GRR39 pKa = 11.84KK40 pKa = 8.88RR41 pKa = 11.84LACC44 pKa = 4.34

Molecular weight: 5.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3807

0

3807

1262493

29

9327

331.6

36.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.44 ± 0.05

1.181 ± 0.019

5.851 ± 0.062

6.072 ± 0.038

3.825 ± 0.031

7.198 ± 0.047

2.195 ± 0.021

6.042 ± 0.031

4.438 ± 0.057

10.505 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.433 ± 0.026

3.868 ± 0.034

3.914 ± 0.024

4.524 ± 0.041

5.041 ± 0.056

7.014 ± 0.045

5.141 ± 0.063

6.999 ± 0.045

1.217 ± 0.014

3.102 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski