Tortoise microvirus 6

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 6.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4P8W6Q7|A0A4P8W6Q7_9VIRU Uncharacterized protein OS=Tortoise microvirus 6 OX=2583165 PE=4 SV=1
MM1 pKa = 7.65HH2 pKa = 7.03NNIYY6 pKa = 10.22IIYY9 pKa = 8.43NVLSKK14 pKa = 10.76RR15 pKa = 11.84YY16 pKa = 8.99GDD18 pKa = 3.6VVAYY22 pKa = 9.65PSDD25 pKa = 3.22AFAARR30 pKa = 11.84RR31 pKa = 11.84LSEE34 pKa = 4.12VYY36 pKa = 9.97KK37 pKa = 10.48DD38 pKa = 3.69HH39 pKa = 7.66PEE41 pKa = 3.89HH42 pKa = 6.2LKK44 pKa = 10.86EE45 pKa = 4.45MEE47 pKa = 4.02LCRR50 pKa = 11.84IGSVSIEE57 pKa = 4.19TGVVTPHH64 pKa = 6.61DD65 pKa = 5.19PIRR68 pKa = 11.84IDD70 pKa = 4.65LDD72 pKa = 3.55VDD74 pKa = 3.64PDD76 pKa = 4.01SPLSLNEE83 pKa = 3.74

Molecular weight: 9.44 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4P8W609|A0A4P8W609_9VIRU DNA pilot protein OS=Tortoise microvirus 6 OX=2583165 PE=4 SV=1
MM1 pKa = 7.08KK2 pKa = 10.13QYY4 pKa = 8.57HH5 pKa = 5.25TSILSLHH12 pKa = 6.28SGCNSRR18 pKa = 11.84YY19 pKa = 8.76CHH21 pKa = 5.75VARR24 pKa = 11.84SAKK27 pKa = 10.2KK28 pKa = 10.04KK29 pKa = 9.96IFHH32 pKa = 6.22LTHH35 pKa = 5.64VKK37 pKa = 10.25KK38 pKa = 10.94SNKK41 pKa = 9.67NYY43 pKa = 8.51PQSNKK48 pKa = 10.09KK49 pKa = 8.98MLTTFFICVILILGGWHH66 pKa = 7.3DD67 pKa = 5.05LYY69 pKa = 11.51NAA71 pKa = 4.33

Molecular weight: 8.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9

0

9

1692

67

572

188.0

21.43

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.678 ± 1.078

1.3 ± 0.518

6.265 ± 0.755

4.787 ± 0.928

3.783 ± 0.72

5.024 ± 0.551

2.128 ± 0.67

6.442 ± 0.68

6.738 ± 1.498

8.688 ± 0.491

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.719 ± 0.379

5.142 ± 0.466

4.078 ± 0.774

6.147 ± 1.441

4.61 ± 0.264

7.861 ± 1.134

6.738 ± 0.832

4.314 ± 0.55

0.827 ± 0.166

5.733 ± 0.659
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski