Enterobacteria phage mEp235

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Hendrixvirinae; Nochtlivirus; Enterobacteria virus mEp235

Average proteome isoelectric point is 7.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 61 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K7PM87|K7PM87_9CAUD Late gene regulator Q OS=Enterobacteria phage mEp235 OX=1147150 GN=mEp235_055 PE=3 SV=1
MM1 pKa = 7.82IDD3 pKa = 3.4PNRR6 pKa = 11.84SYY8 pKa = 10.61EE9 pKa = 3.97QEE11 pKa = 4.12SVEE14 pKa = 5.1RR15 pKa = 11.84ALTCANCGQKK25 pKa = 10.32LHH27 pKa = 5.86VLEE30 pKa = 4.31VHH32 pKa = 6.17VCSDD36 pKa = 4.12CCAEE40 pKa = 5.29LMSDD44 pKa = 4.35PNSSMYY50 pKa = 10.6EE51 pKa = 3.98EE52 pKa = 5.0EE53 pKa = 4.99DD54 pKa = 4.39DD55 pKa = 4.1EE56 pKa = 4.75

Molecular weight: 6.35 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K7PKU3|K7PKU3_9CAUD Rad52/22 family double-strand break repair protein OS=Enterobacteria phage mEp235 OX=1147150 GN=mEp235_032 PE=4 SV=1
MM1 pKa = 7.35YY2 pKa = 8.23LTLQEE7 pKa = 3.73WNARR11 pKa = 11.84QRR13 pKa = 11.84RR14 pKa = 11.84PRR16 pKa = 11.84SLEE19 pKa = 3.71TVRR22 pKa = 11.84RR23 pKa = 11.84WVRR26 pKa = 11.84EE27 pKa = 3.65CRR29 pKa = 11.84IFPPPVKK36 pKa = 10.35DD37 pKa = 3.1GRR39 pKa = 11.84EE40 pKa = 4.02YY41 pKa = 11.07LFHH44 pKa = 6.88EE45 pKa = 4.73SAVKK49 pKa = 10.46VDD51 pKa = 4.11LNRR54 pKa = 11.84PVTDD58 pKa = 3.69SLLKK62 pKa = 10.45RR63 pKa = 11.84IRR65 pKa = 11.84NGKK68 pKa = 8.21KK69 pKa = 10.05AKK71 pKa = 10.06SS72 pKa = 3.44

Molecular weight: 8.69 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

61

0

61

11367

44

1183

186.3

20.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.8 ± 0.458

1.152 ± 0.174

5.929 ± 0.237

5.973 ± 0.3

3.193 ± 0.22

7.548 ± 0.355

1.575 ± 0.248

5.833 ± 0.229

6.044 ± 0.379

7.707 ± 0.339

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.745 ± 0.2

4.381 ± 0.248

3.985 ± 0.251

4.364 ± 0.264

6.255 ± 0.38

6.853 ± 0.352

5.736 ± 0.366

6.246 ± 0.31

1.663 ± 0.131

3.018 ± 0.162
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski