Amino acid dipepetide frequency for Enterobacteria phage mEp235

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.734AlaAla: 10.734 ± 1.855
0.704AlaCys: 0.704 ± 0.271
5.983AlaAsp: 5.983 ± 0.713
5.807AlaGlu: 5.807 ± 0.652
3.167AlaPhe: 3.167 ± 0.621
7.039AlaGly: 7.039 ± 0.83
1.232AlaHis: 1.232 ± 0.356
7.566AlaIle: 7.566 ± 0.819
6.687AlaLys: 6.687 ± 0.792
7.83AlaLeu: 7.83 ± 0.893
4.135AlaMet: 4.135 ± 0.515
4.311AlaAsn: 4.311 ± 0.69
2.112AlaPro: 2.112 ± 0.427
4.135AlaGln: 4.135 ± 0.696
4.575AlaArg: 4.575 ± 0.655
7.302AlaSer: 7.302 ± 0.933
5.191AlaThr: 5.191 ± 0.611
5.279AlaVal: 5.279 ± 0.611
1.32AlaTrp: 1.32 ± 0.309
2.903AlaTyr: 2.903 ± 0.447
0.0AlaXaa: 0.0 ± 0.0
Cys
1.32CysAla: 1.32 ± 0.37
0.264CysCys: 0.264 ± 0.146
0.88CysAsp: 0.88 ± 0.228
0.704CysGlu: 0.704 ± 0.225
0.0CysPhe: 0.0 ± 0.0
0.968CysGly: 0.968 ± 0.38
0.44CysHis: 0.44 ± 0.25
0.616CysIle: 0.616 ± 0.209
0.704CysLys: 0.704 ± 0.261
0.704CysLeu: 0.704 ± 0.26
0.264CysMet: 0.264 ± 0.165
0.352CysAsn: 0.352 ± 0.16
0.352CysPro: 0.352 ± 0.179
0.44CysGln: 0.44 ± 0.183
0.968CysArg: 0.968 ± 0.38
1.144CysSer: 1.144 ± 0.334
0.176CysThr: 0.176 ± 0.106
0.616CysVal: 0.616 ± 0.225
0.44CysTrp: 0.44 ± 0.243
0.176CysTyr: 0.176 ± 0.12
0.0CysXaa: 0.0 ± 0.0
Asp
6.247AspAla: 6.247 ± 0.792
0.792AspCys: 0.792 ± 0.299
4.047AspAsp: 4.047 ± 0.599
2.991AspGlu: 2.991 ± 0.417
2.2AspPhe: 2.2 ± 0.445
4.751AspGly: 4.751 ± 0.702
0.616AspHis: 0.616 ± 0.224
3.255AspIle: 3.255 ± 0.473
4.135AspLys: 4.135 ± 0.679
4.399AspLeu: 4.399 ± 0.728
1.848AspMet: 1.848 ± 0.364
1.936AspAsn: 1.936 ± 0.292
2.727AspPro: 2.727 ± 0.496
2.112AspGln: 2.112 ± 0.445
4.135AspArg: 4.135 ± 0.584
2.991AspSer: 2.991 ± 0.556
2.112AspThr: 2.112 ± 0.4
4.663AspVal: 4.663 ± 0.52
1.056AspTrp: 1.056 ± 0.313
2.288AspTyr: 2.288 ± 0.41
0.0AspXaa: 0.0 ± 0.0
Glu
5.983GluAla: 5.983 ± 0.697
0.792GluCys: 0.792 ± 0.312
2.463GluAsp: 2.463 ± 0.406
3.607GluGlu: 3.607 ± 0.63
2.024GluPhe: 2.024 ± 0.399
3.959GluGly: 3.959 ± 0.743
0.616GluHis: 0.616 ± 0.193
2.903GluIle: 2.903 ± 0.369
3.783GluLys: 3.783 ± 0.527
5.807GluLeu: 5.807 ± 0.62
1.232GluMet: 1.232 ± 0.327
2.991GluAsn: 2.991 ± 0.449
2.288GluPro: 2.288 ± 0.384
3.519GluGln: 3.519 ± 0.513
3.783GluArg: 3.783 ± 0.659
3.959GluSer: 3.959 ± 0.454
3.431GluThr: 3.431 ± 0.433
4.047GluVal: 4.047 ± 0.594
0.792GluTrp: 0.792 ± 0.293
1.76GluTyr: 1.76 ± 0.388
0.0GluXaa: 0.0 ± 0.0
Phe
2.991PheAla: 2.991 ± 0.516
0.176PheCys: 0.176 ± 0.119
2.639PheAsp: 2.639 ± 0.424
2.288PheGlu: 2.288 ± 0.41
0.616PhePhe: 0.616 ± 0.31
2.903PheGly: 2.903 ± 0.38
0.616PheHis: 0.616 ± 0.229
2.112PheIle: 2.112 ± 0.512
1.76PheLys: 1.76 ± 0.312
1.584PheLeu: 1.584 ± 0.323
0.968PheMet: 0.968 ± 0.319
1.672PheAsn: 1.672 ± 0.36
1.32PhePro: 1.32 ± 0.242
0.44PheGln: 0.44 ± 0.171
1.496PheArg: 1.496 ± 0.399
2.463PheSer: 2.463 ± 0.442
2.639PheThr: 2.639 ± 0.425
1.672PheVal: 1.672 ± 0.319
0.528PheTrp: 0.528 ± 0.172
1.056PheTyr: 1.056 ± 0.3
0.0PheXaa: 0.0 ± 0.0
Gly
6.335GlyAla: 6.335 ± 0.868
1.144GlyCys: 1.144 ± 0.312
4.575GlyAsp: 4.575 ± 0.702
4.047GlyGlu: 4.047 ± 0.574
2.815GlyPhe: 2.815 ± 0.504
5.983GlyGly: 5.983 ± 0.746
1.32GlyHis: 1.32 ± 0.317
4.311GlyIle: 4.311 ± 0.551
4.927GlyLys: 4.927 ± 0.603
6.071GlyLeu: 6.071 ± 0.844
2.376GlyMet: 2.376 ± 0.446
4.135GlyAsn: 4.135 ± 0.704
1.056GlyPro: 1.056 ± 0.307
2.991GlyGln: 2.991 ± 0.565
5.543GlyArg: 5.543 ± 0.641
3.607GlySer: 3.607 ± 0.539
5.367GlyThr: 5.367 ± 0.882
5.455GlyVal: 5.455 ± 0.719
1.056GlyTrp: 1.056 ± 0.351
2.376GlyTyr: 2.376 ± 0.38
0.0GlyXaa: 0.0 ± 0.0
His
1.496HisAla: 1.496 ± 0.388
0.352HisCys: 0.352 ± 0.158
0.792HisAsp: 0.792 ± 0.234
1.056HisGlu: 1.056 ± 0.338
0.792HisPhe: 0.792 ± 0.286
1.056HisGly: 1.056 ± 0.26
0.616HisHis: 0.616 ± 0.218
0.88HisIle: 0.88 ± 0.305
0.968HisLys: 0.968 ± 0.288
1.144HisLeu: 1.144 ± 0.373
0.176HisMet: 0.176 ± 0.104
0.528HisAsn: 0.528 ± 0.175
0.704HisPro: 0.704 ± 0.256
0.792HisGln: 0.792 ± 0.289
1.408HisArg: 1.408 ± 0.367
1.144HisSer: 1.144 ± 0.35
0.44HisThr: 0.44 ± 0.22
1.144HisVal: 1.144 ± 0.401
0.088HisTrp: 0.088 ± 0.088
0.176HisTyr: 0.176 ± 0.114
0.0HisXaa: 0.0 ± 0.0
Ile
5.367IleAla: 5.367 ± 0.609
1.056IleCys: 1.056 ± 0.286
4.575IleAsp: 4.575 ± 0.565
3.783IleGlu: 3.783 ± 0.578
2.024IlePhe: 2.024 ± 0.442
4.575IleGly: 4.575 ± 0.65
1.232IleHis: 1.232 ± 0.315
3.079IleIle: 3.079 ± 0.609
2.639IleLys: 2.639 ± 0.392
4.311IleLeu: 4.311 ± 0.484
1.144IleMet: 1.144 ± 0.351
2.727IleAsn: 2.727 ± 0.488
2.024IlePro: 2.024 ± 0.323
2.815IleGln: 2.815 ± 0.587
4.047IleArg: 4.047 ± 0.709
4.399IleSer: 4.399 ± 0.636
3.519IleThr: 3.519 ± 0.583
2.288IleVal: 2.288 ± 0.385
0.704IleTrp: 0.704 ± 0.233
2.024IleTyr: 2.024 ± 0.352
0.0IleXaa: 0.0 ± 0.0
Lys
5.543LysAla: 5.543 ± 0.765
0.528LysCys: 0.528 ± 0.222
3.079LysAsp: 3.079 ± 0.48
3.959LysGlu: 3.959 ± 0.675
1.496LysPhe: 1.496 ± 0.35
4.311LysGly: 4.311 ± 0.511
0.968LysHis: 0.968 ± 0.365
3.255LysIle: 3.255 ± 0.457
4.487LysLys: 4.487 ± 0.708
5.279LysLeu: 5.279 ± 0.814
1.76LysMet: 1.76 ± 0.421
2.288LysAsn: 2.288 ± 0.389
2.903LysPro: 2.903 ± 0.53
3.167LysGln: 3.167 ± 0.64
3.695LysArg: 3.695 ± 0.736
3.431LysSer: 3.431 ± 0.573
3.255LysThr: 3.255 ± 0.453
3.519LysVal: 3.519 ± 0.448
1.408LysTrp: 1.408 ± 0.261
2.024LysTyr: 2.024 ± 0.32
0.0LysXaa: 0.0 ± 0.0
Leu
8.182LeuAla: 8.182 ± 1.024
0.968LeuCys: 0.968 ± 0.3
5.191LeuAsp: 5.191 ± 0.575
5.191LeuGlu: 5.191 ± 0.674
2.024LeuPhe: 2.024 ± 0.412
4.047LeuGly: 4.047 ± 0.582
1.056LeuHis: 1.056 ± 0.348
3.959LeuIle: 3.959 ± 0.549
4.663LeuLys: 4.663 ± 0.676
5.543LeuLeu: 5.543 ± 0.801
2.024LeuMet: 2.024 ± 0.477
3.431LeuAsn: 3.431 ± 0.489
4.047LeuPro: 4.047 ± 0.663
3.431LeuGln: 3.431 ± 0.564
5.015LeuArg: 5.015 ± 0.505
6.335LeuSer: 6.335 ± 0.623
5.015LeuThr: 5.015 ± 0.604
4.223LeuVal: 4.223 ± 0.595
0.616LeuTrp: 0.616 ± 0.189
2.112LeuTyr: 2.112 ± 0.431
0.0LeuXaa: 0.0 ± 0.0
Met
3.167MetAla: 3.167 ± 0.501
0.088MetCys: 0.088 ± 0.101
1.672MetAsp: 1.672 ± 0.346
0.88MetGlu: 0.88 ± 0.262
0.528MetPhe: 0.528 ± 0.206
1.672MetGly: 1.672 ± 0.296
0.352MetHis: 0.352 ± 0.174
1.76MetIle: 1.76 ± 0.43
2.2MetLys: 2.2 ± 0.396
2.463MetLeu: 2.463 ± 0.41
0.968MetMet: 0.968 ± 0.321
1.056MetAsn: 1.056 ± 0.306
1.408MetPro: 1.408 ± 0.313
1.144MetGln: 1.144 ± 0.309
1.232MetArg: 1.232 ± 0.246
2.727MetSer: 2.727 ± 0.545
1.408MetThr: 1.408 ± 0.368
1.848MetVal: 1.848 ± 0.463
0.264MetTrp: 0.264 ± 0.171
0.616MetTyr: 0.616 ± 0.24
0.0MetXaa: 0.0 ± 0.0
Asn
4.575AsnAla: 4.575 ± 0.744
0.616AsnCys: 0.616 ± 0.25
2.024AsnAsp: 2.024 ± 0.356
2.903AsnGlu: 2.903 ± 0.436
1.144AsnPhe: 1.144 ± 0.371
4.839AsnGly: 4.839 ± 0.737
1.144AsnHis: 1.144 ± 0.372
3.431AsnIle: 3.431 ± 0.471
2.551AsnLys: 2.551 ± 0.437
2.551AsnLeu: 2.551 ± 0.554
0.792AsnMet: 0.792 ± 0.248
1.76AsnAsn: 1.76 ± 0.438
2.727AsnPro: 2.727 ± 0.503
1.76AsnGln: 1.76 ± 0.446
1.848AsnArg: 1.848 ± 0.549
2.2AsnSer: 2.2 ± 0.5
3.167AsnThr: 3.167 ± 0.517
1.584AsnVal: 1.584 ± 0.502
0.704AsnTrp: 0.704 ± 0.217
1.496AsnTyr: 1.496 ± 0.401
0.0AsnXaa: 0.0 ± 0.0
Pro
3.871ProAla: 3.871 ± 0.766
0.616ProCys: 0.616 ± 0.242
2.376ProAsp: 2.376 ± 0.346
3.167ProGlu: 3.167 ± 0.558
1.936ProPhe: 1.936 ± 0.358
3.431ProGly: 3.431 ± 0.416
0.528ProHis: 0.528 ± 0.229
1.584ProIle: 1.584 ± 0.41
2.463ProLys: 2.463 ± 0.49
2.815ProLeu: 2.815 ± 0.662
1.496ProMet: 1.496 ± 0.365
1.496ProAsn: 1.496 ± 0.364
1.76ProPro: 1.76 ± 0.423
1.76ProGln: 1.76 ± 0.381
2.112ProArg: 2.112 ± 0.428
2.551ProSer: 2.551 ± 0.449
1.76ProThr: 1.76 ± 0.406
2.639ProVal: 2.639 ± 0.4
0.528ProTrp: 0.528 ± 0.203
0.968ProTyr: 0.968 ± 0.248
0.0ProXaa: 0.0 ± 0.0
Gln
4.047GlnAla: 4.047 ± 0.72
0.44GlnCys: 0.44 ± 0.226
1.32GlnAsp: 1.32 ± 0.355
2.288GlnGlu: 2.288 ± 0.365
1.408GlnPhe: 1.408 ± 0.34
1.76GlnGly: 1.76 ± 0.343
0.792GlnHis: 0.792 ± 0.284
2.815GlnIle: 2.815 ± 0.444
3.079GlnLys: 3.079 ± 0.635
3.431GlnLeu: 3.431 ± 0.538
1.408GlnMet: 1.408 ± 0.279
2.024GlnAsn: 2.024 ± 0.45
1.496GlnPro: 1.496 ± 0.317
2.815GlnGln: 2.815 ± 0.566
3.255GlnArg: 3.255 ± 0.438
3.343GlnSer: 3.343 ± 0.547
2.991GlnThr: 2.991 ± 0.662
2.551GlnVal: 2.551 ± 0.465
0.968GlnTrp: 0.968 ± 0.281
1.408GlnTyr: 1.408 ± 0.413
0.0GlnXaa: 0.0 ± 0.0
Arg
5.015ArgAla: 5.015 ± 0.586
0.704ArgCys: 0.704 ± 0.269
3.783ArgAsp: 3.783 ± 0.625
4.047ArgGlu: 4.047 ± 0.758
2.376ArgPhe: 2.376 ± 0.489
3.783ArgGly: 3.783 ± 0.682
1.144ArgHis: 1.144 ± 0.324
3.871ArgIle: 3.871 ± 0.558
4.223ArgLys: 4.223 ± 0.61
5.103ArgLeu: 5.103 ± 0.619
1.496ArgMet: 1.496 ± 0.392
3.255ArgAsn: 3.255 ± 0.524
2.551ArgPro: 2.551 ± 0.541
2.639ArgGln: 2.639 ± 0.458
4.575ArgArg: 4.575 ± 1.003
3.343ArgSer: 3.343 ± 0.583
2.727ArgThr: 2.727 ± 0.478
3.871ArgVal: 3.871 ± 0.599
1.144ArgTrp: 1.144 ± 0.275
2.903ArgTyr: 2.903 ± 0.439
0.0ArgXaa: 0.0 ± 0.0
Ser
6.247SerAla: 6.247 ± 0.645
0.352SerCys: 0.352 ± 0.17
4.311SerAsp: 4.311 ± 0.5
3.959SerGlu: 3.959 ± 0.548
2.376SerPhe: 2.376 ± 0.401
6.863SerGly: 6.863 ± 0.763
0.616SerHis: 0.616 ± 0.208
3.343SerIle: 3.343 ± 0.487
3.167SerLys: 3.167 ± 0.687
5.895SerLeu: 5.895 ± 0.737
1.32SerMet: 1.32 ± 0.36
2.991SerAsn: 2.991 ± 0.524
3.255SerPro: 3.255 ± 0.567
2.288SerGln: 2.288 ± 0.46
4.487SerArg: 4.487 ± 0.717
5.631SerSer: 5.631 ± 1.084
3.519SerThr: 3.519 ± 0.484
5.103SerVal: 5.103 ± 1.008
1.144SerTrp: 1.144 ± 0.27
1.672SerTyr: 1.672 ± 0.348
0.0SerXaa: 0.0 ± 0.0
Thr
7.039ThrAla: 7.039 ± 0.932
0.616ThrCys: 0.616 ± 0.226
3.343ThrAsp: 3.343 ± 0.502
3.783ThrGlu: 3.783 ± 0.562
1.848ThrPhe: 1.848 ± 0.465
5.807ThrGly: 5.807 ± 0.819
0.528ThrHis: 0.528 ± 0.216
2.991ThrIle: 2.991 ± 0.504
2.288ThrLys: 2.288 ± 0.562
4.311ThrLeu: 4.311 ± 0.666
0.88ThrMet: 0.88 ± 0.249
1.76ThrAsn: 1.76 ± 0.357
2.815ThrPro: 2.815 ± 0.596
2.903ThrGln: 2.903 ± 0.481
2.727ThrArg: 2.727 ± 0.488
3.519ThrSer: 3.519 ± 0.628
3.167ThrThr: 3.167 ± 0.48
4.135ThrVal: 4.135 ± 0.47
1.232ThrTrp: 1.232 ± 0.339
1.672ThrTyr: 1.672 ± 0.383
0.0ThrXaa: 0.0 ± 0.0
Val
5.455ValAla: 5.455 ± 0.727
0.528ValCys: 0.528 ± 0.239
4.047ValAsp: 4.047 ± 0.581
3.255ValGlu: 3.255 ± 0.521
1.848ValPhe: 1.848 ± 0.305
4.223ValGly: 4.223 ± 0.779
0.792ValHis: 0.792 ± 0.237
4.663ValIle: 4.663 ± 0.594
3.695ValLys: 3.695 ± 0.578
4.575ValLeu: 4.575 ± 0.7
1.936ValMet: 1.936 ± 0.323
3.607ValAsn: 3.607 ± 0.617
2.376ValPro: 2.376 ± 0.483
2.024ValGln: 2.024 ± 0.386
3.343ValArg: 3.343 ± 0.417
5.015ValSer: 5.015 ± 0.715
4.487ValThr: 4.487 ± 0.595
3.959ValVal: 3.959 ± 0.626
1.32ValTrp: 1.32 ± 0.298
1.32ValTyr: 1.32 ± 0.279
0.0ValXaa: 0.0 ± 0.0
Trp
1.32TrpAla: 1.32 ± 0.283
0.352TrpCys: 0.352 ± 0.182
0.968TrpAsp: 0.968 ± 0.264
0.704TrpGlu: 0.704 ± 0.212
0.616TrpPhe: 0.616 ± 0.256
1.32TrpGly: 1.32 ± 0.34
0.616TrpHis: 0.616 ± 0.199
0.264TrpIle: 0.264 ± 0.147
0.704TrpLys: 0.704 ± 0.188
1.32TrpLeu: 1.32 ± 0.421
0.616TrpMet: 0.616 ± 0.223
0.352TrpAsn: 0.352 ± 0.162
0.44TrpPro: 0.44 ± 0.157
0.88TrpGln: 0.88 ± 0.217
1.408TrpArg: 1.408 ± 0.383
0.88TrpSer: 0.88 ± 0.234
0.968TrpThr: 0.968 ± 0.444
1.848TrpVal: 1.848 ± 0.317
0.528TrpTrp: 0.528 ± 0.213
0.528TrpTyr: 0.528 ± 0.201
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.079TyrAla: 3.079 ± 0.434
0.44TyrCys: 0.44 ± 0.156
1.232TyrAsp: 1.232 ± 0.247
1.32TyrGlu: 1.32 ± 0.253
0.704TyrPhe: 0.704 ± 0.236
2.2TyrGly: 2.2 ± 0.416
0.528TyrHis: 0.528 ± 0.256
1.672TyrIle: 1.672 ± 0.339
1.32TyrLys: 1.32 ± 0.338
1.936TyrLeu: 1.936 ± 0.388
0.528TyrMet: 0.528 ± 0.205
1.144TyrAsn: 1.144 ± 0.261
1.496TyrPro: 1.496 ± 0.339
1.584TyrGln: 1.584 ± 0.383
2.903TyrArg: 2.903 ± 0.516
2.551TyrSer: 2.551 ± 0.505
2.024TyrThr: 2.024 ± 0.492
2.024TyrVal: 2.024 ± 0.313
0.792TyrTrp: 0.792 ± 0.21
0.704TyrTyr: 0.704 ± 0.209
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 61 proteins (11367 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski