Planktothrix agardhii NIVA-CYA 126/8

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Oscillatoriophycideae; Oscillatoriales; Microcoleaceae; Planktothrix; Planktothrix agardhii

Average proteome isoelectric point is 6.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4186 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A073CS64|A0A073CS64_PLAAG Uma2 domain-containing protein OS=Planktothrix agardhii NIVA-CYA 126/8 OX=388467 GN=A19Y_1855 PE=4 SV=1
MM1 pKa = 7.86IDD3 pKa = 3.05TGVDD7 pKa = 3.48YY8 pKa = 11.13NHH10 pKa = 7.86PDD12 pKa = 3.2LSANIWTNPGEE23 pKa = 4.15IAGDD27 pKa = 4.26GIDD30 pKa = 4.09NDD32 pKa = 4.3SNGYY36 pKa = 9.52IDD38 pKa = 6.35DD39 pKa = 3.64VRR41 pKa = 11.84GWDD44 pKa = 4.12FAYY47 pKa = 11.06NDD49 pKa = 4.04NNPMDD54 pKa = 3.75VHH56 pKa = 6.17GHH58 pKa = 4.65GTHH61 pKa = 5.77VAGTIAGKK69 pKa = 10.46GNNGVGVTGVAWNAKK84 pKa = 8.92IMPLKK89 pKa = 10.31FLNDD93 pKa = 3.77SGWGYY98 pKa = 9.31TSDD101 pKa = 4.48AISAINYY108 pKa = 7.05ATAKK112 pKa = 9.97GVKK115 pKa = 8.44LTNNSWGGDD124 pKa = 3.48GYY126 pKa = 8.95TQALYY131 pKa = 10.77DD132 pKa = 4.8AINTAGQQGALFIAAAGNSSQNTDD156 pKa = 3.12TTPAYY161 pKa = 8.05PASYY165 pKa = 10.52DD166 pKa = 3.45LANIISVASTTRR178 pKa = 11.84TDD180 pKa = 3.34GLSWFSNYY188 pKa = 9.78GATTVDD194 pKa = 3.78LGAPGSDD201 pKa = 4.69IYY203 pKa = 11.2SAWPNSTYY211 pKa = 10.03NTISGTSMASPHH223 pKa = 4.84VTGAAALLWSQNPTWTAQQVKK244 pKa = 9.5NRR246 pKa = 11.84LMSTGDD252 pKa = 3.92SISALNGKK260 pKa = 6.36TVSGKK265 pKa = 10.11RR266 pKa = 11.84LNIYY270 pKa = 9.14NALMSSNLPTVTVNVSPVTVQEE292 pKa = 4.68DD293 pKa = 4.11GSGNLTYY300 pKa = 10.83SFSRR304 pKa = 11.84SGNLTSAMTVNFGVAGTANAAAVGSDD330 pKa = 3.14PADD333 pKa = 3.39YY334 pKa = 10.1TVLTNSAVTFSPSTKK349 pKa = 9.75TGTITFAAGSSTAQLVVDD367 pKa = 4.64PTADD371 pKa = 3.31TLAEE375 pKa = 4.26SQNEE379 pKa = 4.0TVVFNINSGTGYY391 pKa = 10.33IGGTPNTATGTIVSEE406 pKa = 4.03EE407 pKa = 3.96VLPIFTNPNSITIPSSGSASPYY429 pKa = 9.02PSTINVSGVSGNIANIQVSLSGLSHH454 pKa = 6.84TWPDD458 pKa = 3.69DD459 pKa = 3.04VDD461 pKa = 3.54MFLRR465 pKa = 11.84GPGGQKK471 pKa = 10.42VMLMSDD477 pKa = 3.22AGDD480 pKa = 3.49FADD483 pKa = 4.92LNNVNLTFSDD493 pKa = 4.07SASGTLPDD501 pKa = 4.54GSQITSGTYY510 pKa = 9.82RR511 pKa = 11.84PTDD514 pKa = 3.6YY515 pKa = 10.91QVGDD519 pKa = 4.12TFPTPAPAGPYY530 pKa = 8.21GTALSAFNGTNPNGAWQLFVQDD552 pKa = 3.58DD553 pKa = 4.21TGWDD557 pKa = 3.45SGSIAGGWSLTIQRR571 pKa = 11.84TSTINGTAGADD582 pKa = 3.73NLIGTANPDD591 pKa = 3.91IINGLAGNDD600 pKa = 3.72TLNGNTGADD609 pKa = 3.58TLVGGLGNDD618 pKa = 3.01IYY620 pKa = 11.73VVDD623 pKa = 4.03NTGDD627 pKa = 3.29IATEE631 pKa = 4.01LASQGTDD638 pKa = 4.11LIQSSVTYY646 pKa = 8.07TLPANVEE653 pKa = 4.22DD654 pKa = 4.1LTLTGTTAINGTGNALANIITGNTANNILDD684 pKa = 4.01GSSGADD690 pKa = 3.17QLKK693 pKa = 10.74GGTGNDD699 pKa = 3.36TYY701 pKa = 11.75VVDD704 pKa = 3.68NTGDD708 pKa = 3.54VVTEE712 pKa = 4.42LASQGTDD719 pKa = 4.11LIQSSVTYY727 pKa = 8.07TLPANVEE734 pKa = 4.22DD735 pKa = 4.1LTLTGTTAINGTGNTVANIITGNTANNILDD765 pKa = 4.01GSSGADD771 pKa = 3.17QLKK774 pKa = 10.74GGTGNDD780 pKa = 3.36TYY782 pKa = 11.75VVDD785 pKa = 3.68NTGDD789 pKa = 3.54VVTEE793 pKa = 4.42LASQGTDD800 pKa = 4.11LIQSSVTYY808 pKa = 8.07TLPANVEE815 pKa = 4.22DD816 pKa = 4.1LTLTGTTAINGTGNTLANTVTGNTANNILNGGTGNDD852 pKa = 3.47NLIGGSGTDD861 pKa = 3.31QLLGSDD867 pKa = 4.59GNDD870 pKa = 3.42SLSGDD875 pKa = 3.94AGNDD879 pKa = 3.36TLTGGLGADD888 pKa = 3.14KK889 pKa = 10.75FIYY892 pKa = 8.36NTNAAFTTTAVGVDD906 pKa = 4.14TITDD910 pKa = 3.82FNISQTDD917 pKa = 3.49QIVLDD922 pKa = 3.81KK923 pKa = 9.4TTFTSISSAAGTGFSVASEE942 pKa = 4.15FAKK945 pKa = 9.75VTSDD949 pKa = 3.32ALAATSAADD958 pKa = 3.27IVYY961 pKa = 8.13NTATGGLFYY970 pKa = 10.7NQNGTAAGLGTGAQFLTLTNKK991 pKa = 9.62PALTATQFLIQAA1003 pKa = 4.57

Molecular weight: 101.89 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A073CMB1|A0A073CMB1_PLAAG Isoamyl acetate-hydrolyzing esterase 1 OS=Planktothrix agardhii NIVA-CYA 126/8 OX=388467 GN=A19Y_4130 PE=4 SV=1
MM1 pKa = 7.64EE2 pKa = 5.6KK3 pKa = 10.02SQKK6 pKa = 9.93IKK8 pKa = 11.14LDD10 pKa = 3.24MNRR13 pKa = 11.84NKK15 pKa = 10.44KK16 pKa = 7.37FTKK19 pKa = 10.3GATLFLTGLATVGLVLGEE37 pKa = 4.48AVFSSASAAQQLFCNGRR54 pKa = 11.84MNNGWTYY61 pKa = 11.11SAQFLNGRR69 pKa = 11.84FTEE72 pKa = 4.25IRR74 pKa = 11.84WEE76 pKa = 4.1RR77 pKa = 11.84SGQPPIVSNLSFQSTNNARR96 pKa = 11.84QPVYY100 pKa = 9.67TGSIMAATMVTLVDD114 pKa = 4.15LSRR117 pKa = 11.84GNVRR121 pKa = 11.84PGSQISVTAEE131 pKa = 3.32EE132 pKa = 4.9WGTSTGNCGLSSGNSMGNSSDD153 pKa = 2.68WFTNARR159 pKa = 11.84KK160 pKa = 9.58NLIGSNEE167 pKa = 3.82NQARR171 pKa = 11.84RR172 pKa = 11.84WLTRR176 pKa = 11.84NNFVFNQTMEE186 pKa = 4.02HH187 pKa = 5.15TNRR190 pKa = 11.84RR191 pKa = 11.84IIEE194 pKa = 3.81RR195 pKa = 11.84WSRR198 pKa = 11.84NNPNGVIDD206 pKa = 4.46VIFVNNRR213 pKa = 11.84VSDD216 pKa = 3.9VVMVRR221 pKa = 3.56

Molecular weight: 24.64 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4186

0

4186

1394687

28

4714

333.2

37.34

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.87 ± 0.034

0.968 ± 0.011

4.985 ± 0.028

6.498 ± 0.039

4.05 ± 0.026

6.548 ± 0.04

1.751 ± 0.016

7.499 ± 0.033

5.131 ± 0.032

11.151 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.816 ± 0.017

4.783 ± 0.035

4.921 ± 0.031

5.678 ± 0.036

4.594 ± 0.028

6.408 ± 0.031

5.686 ± 0.033

6.086 ± 0.032

1.407 ± 0.015

3.169 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski