Kitasatospora aureofaciens (Streptomyces aureofaciens)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Kitasatospora

Average proteome isoelectric point is 6.38

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7620 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1E7N186|A0A1E7N186_KITAU Enolase OS=Kitasatospora aureofaciens OX=1894 GN=eno PE=3 SV=1
MM1 pKa = 7.49PVVTDD6 pKa = 4.33PYY8 pKa = 11.32KK9 pKa = 10.75PGTPCWIDD17 pKa = 4.43LMASDD22 pKa = 4.32QQAALDD28 pKa = 4.19FYY30 pKa = 11.32RR31 pKa = 11.84DD32 pKa = 3.38LFGWQGEE39 pKa = 4.17IGPAEE44 pKa = 3.93FGGYY48 pKa = 9.01AVCTLGGRR56 pKa = 11.84PVAGIMAQSGPEE68 pKa = 4.17GQPLPPVAWTTYY80 pKa = 9.88LASDD84 pKa = 4.13DD85 pKa = 3.71VDD87 pKa = 3.34AARR90 pKa = 11.84AAIAGAGGTVLYY102 pKa = 11.0DD103 pKa = 3.67PMDD106 pKa = 3.7VGALGRR112 pKa = 11.84MMVAADD118 pKa = 3.32PTGAVFGVWQAMDD131 pKa = 4.24FIGAGVVNEE140 pKa = 4.37PGALVWNEE148 pKa = 4.27LNTADD153 pKa = 3.82TGAAGGFYY161 pKa = 10.25QSALGLRR168 pKa = 11.84PATIQGMDD176 pKa = 3.43GYY178 pKa = 11.37YY179 pKa = 10.32SLNVGDD185 pKa = 3.86RR186 pKa = 11.84TVGGMQEE193 pKa = 3.66IPKK196 pKa = 9.98YY197 pKa = 10.37LDD199 pKa = 3.53AGTPSHH205 pKa = 6.32WMPYY209 pKa = 10.11FSVDD213 pKa = 3.46DD214 pKa = 3.83TDD216 pKa = 4.15SVVDD220 pKa = 4.66ALVKK224 pKa = 10.72QNGTVVQPPFDD235 pKa = 3.83MQSGRR240 pKa = 11.84MAVVKK245 pKa = 10.21DD246 pKa = 3.4PQGAVFAVIQAPEE259 pKa = 3.94AQLPDD264 pKa = 3.68SPP266 pKa = 4.86

Molecular weight: 27.83 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1E7NB81|A0A1E7NB81_KITAU DNA-binding response regulator OS=Kitasatospora aureofaciens OX=1894 GN=B6264_14705 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILAARR35 pKa = 11.84RR36 pKa = 11.84GKK38 pKa = 10.43GRR40 pKa = 11.84ASLSAA45 pKa = 3.83

Molecular weight: 5.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7620

0

7620

2446950

29

9000

321.1

34.24

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.12 ± 0.047

0.794 ± 0.008

5.754 ± 0.02

5.527 ± 0.028

2.664 ± 0.014

9.604 ± 0.028

2.326 ± 0.015

2.94 ± 0.02

1.833 ± 0.024

10.794 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.571 ± 0.013

1.79 ± 0.019

6.392 ± 0.031

2.903 ± 0.019

8.0 ± 0.036

4.867 ± 0.024

6.155 ± 0.026

8.43 ± 0.026

1.524 ± 0.013

2.013 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski