Altererythrobacter sp. HN-Y73

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Erythrobacteraceae; Altererythrobacter

Average proteome isoelectric point is 6.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3349 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A420ERE8|A0A420ERE8_9SPHN M3 family peptidase OS=Altererythrobacter sp. HN-Y73 OX=2320269 GN=D6851_01700 PE=3 SV=1
MM1 pKa = 7.52LALSACGPEE10 pKa = 4.93EE11 pKa = 3.8ISSPGSGGDD20 pKa = 3.51VIINPSPTPGPTPTPTPGSSGSYY43 pKa = 8.65TAAEE47 pKa = 4.29SCPDD51 pKa = 3.16IGDD54 pKa = 3.83PAGLSDD60 pKa = 3.78GGVINVPGGTVRR72 pKa = 11.84SCVLPGTFTVSSSLPQTEE90 pKa = 4.73DD91 pKa = 3.01NAKK94 pKa = 9.64VVYY97 pKa = 9.53EE98 pKa = 3.63ISGAVNVGDD107 pKa = 5.43DD108 pKa = 3.79ADD110 pKa = 4.15ADD112 pKa = 4.2YY113 pKa = 11.6EE114 pKa = 4.53DD115 pKa = 5.59GEE117 pKa = 4.82TPATNVVLTIDD128 pKa = 3.68PGVVLFGGTGNSYY141 pKa = 9.69MVVNRR146 pKa = 11.84GNKK149 pKa = 8.18INAVGTVDD157 pKa = 3.58APIIFTSRR165 pKa = 11.84QNILGNTSDD174 pKa = 5.33DD175 pKa = 4.53VDD177 pKa = 4.2GQWGGVILLGRR188 pKa = 11.84APISDD193 pKa = 4.09CGATGITGGTAEE205 pKa = 5.23CEE207 pKa = 4.03NQIEE211 pKa = 4.56GVATGGYY218 pKa = 10.08FGGDD222 pKa = 2.77QDD224 pKa = 4.39ADD226 pKa = 3.23NSGVMKK232 pKa = 10.02YY233 pKa = 8.67FQIRR237 pKa = 11.84YY238 pKa = 9.54SGFAIAKK245 pKa = 9.84DD246 pKa = 3.75NEE248 pKa = 4.43LQSLTTGGTGHH259 pKa = 5.93NTEE262 pKa = 3.58IDD264 pKa = 3.68YY265 pKa = 9.87FQSFNSSDD273 pKa = 3.43DD274 pKa = 3.23GMEE277 pKa = 3.93FFGGYY282 pKa = 10.1VNMKK286 pKa = 8.74HH287 pKa = 5.77AVVVGASDD295 pKa = 4.94DD296 pKa = 5.1SIDD299 pKa = 3.52TDD301 pKa = 4.31FGVQANMQYY310 pKa = 10.77VIAVQRR316 pKa = 11.84TSTGNGMIEE325 pKa = 4.15ADD327 pKa = 3.37SSEE330 pKa = 4.18NQLRR334 pKa = 11.84PPRR337 pKa = 11.84QDD339 pKa = 2.69TRR341 pKa = 11.84IANATFIHH349 pKa = 6.39QSGSPDD355 pKa = 3.35AAGLRR360 pKa = 11.84YY361 pKa = 9.82RR362 pKa = 11.84GGADD366 pKa = 2.97VSLVNTVMNASSNTCLDD383 pKa = 3.03IDD385 pKa = 3.87QAEE388 pKa = 4.51TFQSDD393 pKa = 3.39SDD395 pKa = 4.39RR396 pKa = 11.84NDD398 pKa = 3.33ATDD401 pKa = 3.77PAPDD405 pKa = 3.22NGAPFFSAVYY415 pKa = 6.8MQCGTAFTDD424 pKa = 5.55DD425 pKa = 3.66GDD427 pKa = 3.91ADD429 pKa = 3.6KK430 pKa = 11.8AEE432 pKa = 4.24AAFGATSNDD441 pKa = 3.34AFSGGVASNASFTTTLNMTFINGTNEE467 pKa = 3.5NGAASWDD474 pKa = 3.27PSVFNRR480 pKa = 11.84NGFTFDD486 pKa = 2.94NVGYY490 pKa = 9.82IGAVRR495 pKa = 11.84DD496 pKa = 4.68EE497 pKa = 4.21NDD499 pKa = 2.26TWYY502 pKa = 10.77QGWTCDD508 pKa = 4.64SSIADD513 pKa = 3.93FGSGSNCADD522 pKa = 3.28SPFNN526 pKa = 3.98

Molecular weight: 54.38 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A420EMC9|A0A420EMC9_9SPHN Uncharacterized protein OS=Altererythrobacter sp. HN-Y73 OX=2320269 GN=D6851_07065 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.51GFRR19 pKa = 11.84ARR21 pKa = 11.84KK22 pKa = 7.81ATVGGRR28 pKa = 11.84KK29 pKa = 8.12VLRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.02KK41 pKa = 10.61LSAA44 pKa = 4.03

Molecular weight: 5.16 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3349

0

3349

1079632

17

4265

322.4

35.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.761 ± 0.059

0.851 ± 0.014

6.24 ± 0.044

6.1 ± 0.045

3.685 ± 0.026

8.524 ± 0.05

2.063 ± 0.026

5.267 ± 0.026

3.324 ± 0.035

9.832 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.53 ± 0.022

2.877 ± 0.027

5.099 ± 0.028

3.406 ± 0.023

6.938 ± 0.051

5.821 ± 0.04

5.248 ± 0.04

6.594 ± 0.038

1.437 ± 0.02

2.403 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski