Polysphondylium pallidum (strain ATCC 26659 / Pp 5 / PN500) (Heterostelium pallidum)

Taxonomy: cellular organisms; Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Acytosteliales; Acytosteliaceae; Heterostelium; Heterostelium album

Average proteome isoelectric point is 6.37

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 12351 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D3BJ12|D3BJ12_POLPP Uncharacterized protein OS=Polysphondylium pallidum (strain ATCC 26659 / Pp 5 / PN500) OX=670386 GN=PPL_08249 PE=4 SV=1
MM1 pKa = 7.54EE2 pKa = 5.55LAFDD6 pKa = 4.12DD7 pKa = 4.29VFTGVFIGDD16 pKa = 4.29DD17 pKa = 3.78DD18 pKa = 6.91ADD20 pKa = 5.28DD21 pKa = 4.65EE22 pKa = 6.24DD23 pKa = 6.26DD24 pKa = 5.89DD25 pKa = 5.02EE26 pKa = 5.35SSPDD30 pKa = 3.4AEE32 pKa = 4.16LSPRR36 pKa = 11.84IILAFEE42 pKa = 4.18LLLIVMCAADD52 pKa = 3.6AAEE55 pKa = 4.84SLSKK59 pKa = 10.82SCVVSDD65 pKa = 3.41ILPYY69 pKa = 9.65STSTLNGSDD78 pKa = 3.11KK79 pKa = 11.13CSGIEE84 pKa = 3.74LFIKK88 pKa = 10.31GIHH91 pKa = 6.28NICLYY96 pKa = 10.31IEE98 pKa = 3.96NRR100 pKa = 11.84YY101 pKa = 6.79TLLYY105 pKa = 10.47RR106 pKa = 11.84VDD108 pKa = 4.24LVII111 pKa = 5.72

Molecular weight: 12.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D3B105|D3B105_POLPP Uncharacterized protein OS=Polysphondylium pallidum (strain ATCC 26659 / Pp 5 / PN500) OX=670386 GN=tipC PE=3 SV=1
MM1 pKa = 6.78STKK4 pKa = 10.29SQGIIIGTRR13 pKa = 11.84RR14 pKa = 11.84SCRR17 pKa = 11.84RR18 pKa = 11.84RR19 pKa = 11.84RR20 pKa = 11.84RR21 pKa = 11.84SRR23 pKa = 11.84RR24 pKa = 11.84NSSSSSRR31 pKa = 11.84RR32 pKa = 11.84RR33 pKa = 11.84SRR35 pKa = 11.84LLLL38 pKa = 3.97

Molecular weight: 4.52 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

12351

0

12351

6384829

16

7412

516.9

58.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.613 ± 0.019

1.459 ± 0.013

5.535 ± 0.017

5.607 ± 0.028

4.31 ± 0.016

4.791 ± 0.024

2.168 ± 0.013

7.365 ± 0.018

6.553 ± 0.025

8.76 ± 0.028

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.0 ± 0.008

7.657 ± 0.031

4.365 ± 0.02

4.931 ± 0.029

3.618 ± 0.016

9.582 ± 0.031

6.748 ± 0.04

5.378 ± 0.018

0.848 ± 0.006

3.711 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski