Amino acid dipepetide frequency for Polysphondylium pallidum (strain ATCC 26659 / Pp 5 / PN500) (Heterostelium pallidum)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	3.673AlaAla: 3.673 ± 0.049	0.673AlaCys: 0.673 ± 0.013	2.156AlaAsp: 2.156 ± 0.02	2.292AlaGlu: 2.292 ± 0.039	1.825AlaPhe: 1.825 ± 0.017	2.403AlaGly: 2.403 ± 0.027	0.827AlaHis: 0.827 ± 0.012	3.639AlaIle: 3.639 ± 0.03	3.023AlaLys: 3.023 ± 0.032	4.032AlaLeu: 4.032 ± 0.035	1.121AlaMet: 1.121 ± 0.016	2.938AlaAsn: 2.938 ± 0.026	2.18AlaPro: 2.18 ± 0.03	1.685AlaGln: 1.685 ± 0.021	1.541AlaArg: 1.541 ± 0.021	4.103AlaSer: 4.103 ± 0.053	3.629AlaThr: 3.629 ± 0.044	2.622AlaVal: 2.622 ± 0.027	0.35AlaTrp: 0.35 ± 0.008	1.416AlaTyr: 1.416 ± 0.015	0.0AlaXaa: 0.0 ± 0.0
Cys	0.638CysAla: 0.638 ± 0.012	0.321CysCys: 0.321 ± 0.013	0.863CysAsp: 0.863 ± 0.024	0.642CysGlu: 0.642 ± 0.012	0.736CysPhe: 0.736 ± 0.012	0.849CysGly: 0.849 ± 0.016	0.33CysHis: 0.33 ± 0.007	1.005CysIle: 1.005 ± 0.013	0.96CysLys: 0.96 ± 0.014	1.301CysLeu: 1.301 ± 0.015	0.243CysMet: 0.243 ± 0.007	0.96CysAsn: 0.96 ± 0.017	0.657CysPro: 0.657 ± 0.017	0.638CysGln: 0.638 ± 0.01	0.558CysArg: 0.558 ± 0.01	1.381CysSer: 1.381 ± 0.021	0.977CysThr: 0.977 ± 0.023	0.784CysVal: 0.784 ± 0.016	0.157CysTrp: 0.157 ± 0.005	0.584CysTyr: 0.584 ± 0.01	0.0CysXaa: 0.0 ± 0.0
Asp	2.232AspAla: 2.232 ± 0.022	0.844AspCys: 0.844 ± 0.013	4.407AspAsp: 4.407 ± 0.046	3.68AspGlu: 3.68 ± 0.034	2.453AspPhe: 2.453 ± 0.022	2.765AspGly: 2.765 ± 0.028	1.282AspHis: 1.282 ± 0.017	4.55AspIle: 4.55 ± 0.033	3.395AspLys: 3.395 ± 0.03	4.69AspLeu: 4.69 ± 0.027	1.032AspMet: 1.032 ± 0.012	3.954AspAsn: 3.954 ± 0.031	2.155AspPro: 2.155 ± 0.023	2.484AspGln: 2.484 ± 0.022	2.382AspArg: 2.382 ± 0.035	4.742AspSer: 4.742 ± 0.035	2.856AspThr: 2.856 ± 0.023	2.698AspVal: 2.698 ± 0.02	0.523AspTrp: 0.523 ± 0.009	2.229AspTyr: 2.229 ± 0.021	0.0AspXaa: 0.0 ± 0.0
Glu	2.385GluAla: 2.385 ± 0.028	0.88GluCys: 0.88 ± 0.013	3.309GluAsp: 3.309 ± 0.034	5.103GluGlu: 5.103 ± 0.079	2.389GluPhe: 2.389 ± 0.022	2.072GluGly: 2.072 ± 0.022	1.122GluHis: 1.122 ± 0.016	4.286GluIle: 4.286 ± 0.031	4.546GluLys: 4.546 ± 0.058	4.918GluLeu: 4.918 ± 0.038	1.4GluMet: 1.4 ± 0.017	3.513GluAsn: 3.513 ± 0.029	1.622GluPro: 1.622 ± 0.022	2.742GluGln: 2.742 ± 0.031	2.622GluArg: 2.622 ± 0.043	4.78GluSer: 4.78 ± 0.036	2.878GluThr: 2.878 ± 0.024	2.703GluVal: 2.703 ± 0.023	0.556GluTrp: 0.556 ± 0.01	2.241GluTyr: 2.241 ± 0.023	0.0GluXaa: 0.0 ± 0.0
Phe	1.874PheAla: 1.874 ± 0.019	0.626PheCys: 0.626 ± 0.011	2.782PheAsp: 2.782 ± 0.024	2.498PheGlu: 2.498 ± 0.02	1.878PhePhe: 1.878 ± 0.023	2.656PheGly: 2.656 ± 0.033	0.853PheHis: 0.853 ± 0.012	3.402PheIle: 3.402 ± 0.028	3.123PheLys: 3.123 ± 0.025	3.518PheLeu: 3.518 ± 0.027	0.81PheMet: 0.81 ± 0.011	3.477PheAsn: 3.477 ± 0.026	1.532PhePro: 1.532 ± 0.017	1.738PheGln: 1.738 ± 0.019	1.405PheArg: 1.405 ± 0.017	3.703PheSer: 3.703 ± 0.024	2.672PheThr: 2.672 ± 0.025	2.443PheVal: 2.443 ± 0.026	0.369PheTrp: 0.369 ± 0.008	1.741PheTyr: 1.741 ± 0.02	0.0PheXaa: 0.0 ± 0.0
Gly	2.569GlyAla: 2.569 ± 0.03	0.935GlyCys: 0.935 ± 0.021	2.707GlyAsp: 2.707 ± 0.025	2.382GlyGlu: 2.382 ± 0.022	2.138GlyPhe: 2.138 ± 0.023	4.174GlyGly: 4.174 ± 0.055	1.185GlyHis: 1.185 ± 0.02	3.167GlyIle: 3.167 ± 0.024	2.733GlyLys: 2.733 ± 0.024	3.601GlyLeu: 3.601 ± 0.032	0.981GlyMet: 0.981 ± 0.016	3.131GlyAsn: 3.131 ± 0.027	1.297GlyPro: 1.297 ± 0.021	1.868GlyGln: 1.868 ± 0.024	1.758GlyArg: 1.758 ± 0.021	4.723GlySer: 4.723 ± 0.045	2.804GlyThr: 2.804 ± 0.034	3.211GlyVal: 3.211 ± 0.029	0.536GlyTrp: 0.536 ± 0.012	2.005GlyTyr: 2.005 ± 0.023	0.0GlyXaa: 0.0 ± 0.0
His	0.756HisAla: 0.756 ± 0.012	0.398HisCys: 0.398 ± 0.009	0.932HisAsp: 0.932 ± 0.013	0.91HisGlu: 0.91 ± 0.015	1.113HisPhe: 1.113 ± 0.013	0.927HisGly: 0.927 ± 0.016	1.175HisHis: 1.175 ± 0.032	1.585HisIle: 1.585 ± 0.019	1.121HisLys: 1.121 ± 0.017	2.264HisLeu: 2.264 ± 0.023	0.387HisMet: 0.387 ± 0.008	1.384HisAsn: 1.384 ± 0.017	1.071HisPro: 1.071 ± 0.016	1.464HisGln: 1.464 ± 0.022	0.996HisArg: 0.996 ± 0.015	2.033HisSer: 2.033 ± 0.021	1.093HisThr: 1.093 ± 0.013	0.924HisVal: 0.924 ± 0.014	0.217HisTrp: 0.217 ± 0.006	0.932HisTyr: 0.932 ± 0.014	0.0HisXaa: 0.0 ± 0.0
Ile	3.475IleAla: 3.475 ± 0.027	1.084IleCys: 1.084 ± 0.015	5.009IleAsp: 5.009 ± 0.032	4.534IleGlu: 4.534 ± 0.036	3.188IlePhe: 3.188 ± 0.029	3.543IleGly: 3.543 ± 0.028	1.519IleHis: 1.519 ± 0.017	5.294IleIle: 5.294 ± 0.034	5.202IleLys: 5.202 ± 0.032	6.066IleLeu: 6.066 ± 0.038	1.241IleMet: 1.241 ± 0.014	5.512IleAsn: 5.512 ± 0.04	3.49IlePro: 3.49 ± 0.028	3.171IleGln: 3.171 ± 0.026	2.626IleArg: 2.626 ± 0.023	6.366IleSer: 6.366 ± 0.05	4.702IleThr: 4.702 ± 0.042	4.368IleVal: 4.368 ± 0.029	0.546IleTrp: 0.546 ± 0.011	2.717IleTyr: 2.717 ± 0.022	0.0IleXaa: 0.0 ± 0.0
Lys	2.632LysAla: 2.632 ± 0.029	0.938LysCys: 0.938 ± 0.017	3.852LysAsp: 3.852 ± 0.031	4.846LysGlu: 4.846 ± 0.05	2.549LysPhe: 2.549 ± 0.021	2.372LysGly: 2.372 ± 0.023	1.377LysHis: 1.377 ± 0.017	4.469LysIle: 4.469 ± 0.028	5.815LysLys: 5.815 ± 0.061	6.007LysLeu: 6.007 ± 0.041	1.478LysMet: 1.478 ± 0.016	3.851LysAsn: 3.851 ± 0.027	2.611LysPro: 2.611 ± 0.029	3.487LysGln: 3.487 ± 0.034	2.924LysArg: 2.924 ± 0.035	6.117LysSer: 6.117 ± 0.044	3.48LysThr: 3.48 ± 0.026	3.38LysVal: 3.38 ± 0.025	0.672LysTrp: 0.672 ± 0.012	2.675LysTyr: 2.675 ± 0.024	0.0LysXaa: 0.0 ± 0.0
Leu	4.36LeuAla: 4.36 ± 0.04	1.193LeuCys: 1.193 ± 0.016	4.934LeuAsp: 4.934 ± 0.028	5.534LeuGlu: 5.534 ± 0.045	4.191LeuPhe: 4.191 ± 0.03	3.534LeuGly: 3.534 ± 0.026	1.874LeuHis: 1.874 ± 0.021	5.927LeuIle: 5.927 ± 0.035	6.505LeuLys: 6.505 ± 0.045	8.463LeuLeu: 8.463 ± 0.061	1.764LeuMet: 1.764 ± 0.018	5.784LeuAsn: 5.784 ± 0.034	4.228LeuPro: 4.228 ± 0.035	3.873LeuGln: 3.873 ± 0.032	3.163LeuArg: 3.163 ± 0.026	8.178LeuSer: 8.178 ± 0.055	5.065LeuThr: 5.065 ± 0.033	5.069LeuVal: 5.069 ± 0.031	0.688LeuTrp: 0.688 ± 0.012	3.279LeuTyr: 3.279 ± 0.028	0.0LeuXaa: 0.0 ± 0.0
Met	1.096MetAla: 1.096 ± 0.014	0.243MetCys: 0.243 ± 0.006	1.245MetAsp: 1.245 ± 0.017	1.275MetGlu: 1.275 ± 0.016	0.874MetPhe: 0.874 ± 0.013	0.913MetGly: 0.913 ± 0.013	0.288MetHis: 0.288 ± 0.007	1.578MetIle: 1.578 ± 0.018	1.521MetLys: 1.521 ± 0.018	1.673MetLeu: 1.673 ± 0.02	0.595MetMet: 0.595 ± 0.012	1.502MetAsn: 1.502 ± 0.016	0.692MetPro: 0.692 ± 0.011	0.592MetGln: 0.592 ± 0.011	0.682MetArg: 0.682 ± 0.012	1.845MetSer: 1.845 ± 0.018	1.338MetThr: 1.338 ± 0.018	1.304MetVal: 1.304 ± 0.016	0.147MetTrp: 0.147 ± 0.005	0.598MetTyr: 0.598 ± 0.01	0.0MetXaa: 0.0 ± 0.0
Asn	2.645AsnAla: 2.645 ± 0.023	0.909AsnCys: 0.909 ± 0.014	3.909AsnAsp: 3.909 ± 0.029	3.521AsnGlu: 3.521 ± 0.026	2.515AsnPhe: 2.515 ± 0.025	3.838AsnGly: 3.838 ± 0.039	1.756AsnHis: 1.756 ± 0.023	5.879AsnIle: 5.879 ± 0.041	4.136AsnLys: 4.136 ± 0.031	5.364AsnLeu: 5.364 ± 0.032	1.203AsnMet: 1.203 ± 0.015	13.725AsnAsn: 13.725 ± 0.178	2.557AsnPro: 2.557 ± 0.025	3.765AsnGln: 3.765 ± 0.033	2.59AsnArg: 2.59 ± 0.023	7.476AsnSer: 7.476 ± 0.053	4.298AsnThr: 4.298 ± 0.034	3.376AsnVal: 3.376 ± 0.03	0.527AsnTrp: 0.527 ± 0.012	2.585AsnTyr: 2.585 ± 0.027	0.0AsnXaa: 0.0 ± 0.0
Pro	2.223ProAla: 2.223 ± 0.024	0.411ProCys: 0.411 ± 0.011	1.854ProAsp: 1.854 ± 0.02	2.054ProGlu: 2.054 ± 0.021	1.818ProPhe: 1.818 ± 0.02	1.634ProGly: 1.634 ± 0.021	0.852ProHis: 0.852 ± 0.013	3.348ProIle: 3.348 ± 0.032	2.634ProLys: 2.634 ± 0.025	3.699ProLeu: 3.699 ± 0.029	0.89ProMet: 0.89 ± 0.012	2.957ProAsn: 2.957 ± 0.029	3.316ProPro: 3.316 ± 0.062	1.955ProGln: 1.955 ± 0.03	1.266ProArg: 1.266 ± 0.02	4.405ProSer: 4.405 ± 0.042	4.174ProThr: 4.174 ± 0.057	2.472ProVal: 2.472 ± 0.024	0.279ProTrp: 0.279 ± 0.005	1.409ProTyr: 1.409 ± 0.018	0.0ProXaa: 0.0 ± 0.0
Gln	1.796GlnAla: 1.796 ± 0.026	0.705GlnCys: 0.705 ± 0.013	1.766GlnAsp: 1.766 ± 0.02	2.183GlnGlu: 2.183 ± 0.025	2.069GlnPhe: 2.069 ± 0.021	1.281GlnGly: 1.281 ± 0.018	1.434GlnHis: 1.434 ± 0.023	2.993GlnIle: 2.993 ± 0.026	2.548GlnLys: 2.548 ± 0.027	4.79GlnLeu: 4.79 ± 0.036	1.015GlnMet: 1.015 ± 0.013	2.442GlnAsn: 2.442 ± 0.025	2.524GlnPro: 2.524 ± 0.035	8.307GlnGln: 8.307 ± 0.17	1.723GlnArg: 1.723 ± 0.02	4.511GlnSer: 4.511 ± 0.034	2.687GlnThr: 2.687 ± 0.026	2.109GlnVal: 2.109 ± 0.02	0.484GlnTrp: 0.484 ± 0.01	1.944GlnTyr: 1.944 ± 0.023	0.0GlnXaa: 0.0 ± 0.0
Arg	1.718ArgAla: 1.718 ± 0.021	0.601ArgCys: 0.601 ± 0.011	2.276ArgAsp: 2.276 ± 0.034	2.36ArgGlu: 2.36 ± 0.038	1.818ArgPhe: 1.818 ± 0.018	1.686ArgGly: 1.686 ± 0.021	0.761ArgHis: 0.761 ± 0.011	2.595ArgIle: 2.595 ± 0.023	2.436ArgLys: 2.436 ± 0.023	3.506ArgLeu: 3.506 ± 0.03	0.797ArgMet: 0.797 ± 0.012	2.102ArgAsn: 2.102 ± 0.019	1.275ArgPro: 1.275 ± 0.016	1.467ArgGln: 1.467 ± 0.018	1.657ArgArg: 1.657 ± 0.021	3.106ArgSer: 3.106 ± 0.027	1.827ArgThr: 1.827 ± 0.019	2.117ArgVal: 2.117 ± 0.021	0.42ArgTrp: 0.42 ± 0.008	1.651ArgTyr: 1.651 ± 0.016	0.0ArgXaa: 0.0 ± 0.0
Ser	3.923SerAla: 3.923 ± 0.033	1.169SerCys: 1.169 ± 0.023	4.44SerAsp: 4.44 ± 0.032	3.864SerGlu: 3.864 ± 0.03	4.084SerPhe: 4.084 ± 0.03	4.758SerGly: 4.758 ± 0.048	1.734SerHis: 1.734 ± 0.022	7.624SerIle: 7.624 ± 0.049	5.848SerLys: 5.848 ± 0.04	8.5SerLeu: 8.5 ± 0.051	1.919SerMet: 1.919 ± 0.018	8.023SerAsn: 8.023 ± 0.057	4.494SerPro: 4.494 ± 0.049	3.494SerGln: 3.494 ± 0.029	2.779SerArg: 2.779 ± 0.029	12.959SerSer: 12.959 ± 0.111	7.799SerThr: 7.799 ± 0.1	4.679SerVal: 4.679 ± 0.04	0.644SerTrp: 0.644 ± 0.012	3.085SerTyr: 3.085 ± 0.027	0.0SerXaa: 0.0 ± 0.0
Thr	3.284ThrAla: 3.284 ± 0.057	0.848ThrCys: 0.848 ± 0.018	2.921ThrAsp: 2.921 ± 0.024	2.801ThrGlu: 2.801 ± 0.026	2.682ThrPhe: 2.682 ± 0.024	3.152ThrGly: 3.152 ± 0.053	1.119ThrHis: 1.119 ± 0.013	5.198ThrIle: 5.198 ± 0.05	3.809ThrLys: 3.809 ± 0.03	5.945ThrLeu: 5.945 ± 0.042	1.221ThrMet: 1.221 ± 0.017	4.522ThrAsn: 4.522 ± 0.037	3.905ThrPro: 3.905 ± 0.047	2.225ThrGln: 2.225 ± 0.024	1.72ThrArg: 1.72 ± 0.016	6.834ThrSer: 6.834 ± 0.069	9.397ThrThr: 9.397 ± 0.179	3.478ThrVal: 3.478 ± 0.037	0.418ThrTrp: 0.418 ± 0.008	2.001ThrTyr: 2.001 ± 0.021	0.0ThrXaa: 0.0 ± 0.0
Val	3.068ValAla: 3.068 ± 0.026	0.927ValCys: 0.927 ± 0.015	3.43ValAsp: 3.43 ± 0.025	3.391ValGlu: 3.391 ± 0.029	2.379ValPhe: 2.379 ± 0.021	3.084ValGly: 3.084 ± 0.026	1.011ValHis: 1.011 ± 0.013	3.687ValIle: 3.687 ± 0.027	3.427ValLys: 3.427 ± 0.026	4.576ValLeu: 4.576 ± 0.033	1.009ValMet: 1.009 ± 0.013	3.446ValAsn: 3.446 ± 0.029	2.307ValPro: 2.307 ± 0.023	2.072ValGln: 2.072 ± 0.02	1.842ValArg: 1.842 ± 0.018	4.483ValSer: 4.483 ± 0.034	3.301ValThr: 3.301 ± 0.038	3.929ValVal: 3.929 ± 0.036	0.486ValTrp: 0.486 ± 0.01	1.927ValTyr: 1.927 ± 0.021	0.0ValXaa: 0.0 ± 0.0
Trp	0.377TrpAla: 0.377 ± 0.009	0.162TrpCys: 0.162 ± 0.004	0.493TrpAsp: 0.493 ± 0.01	0.429TrpGlu: 0.429 ± 0.009	0.433TrpPhe: 0.433 ± 0.009	0.389TrpGly: 0.389 ± 0.009	0.144TrpHis: 0.144 ± 0.005	0.698TrpIle: 0.698 ± 0.012	0.616TrpLys: 0.616 ± 0.01	0.854TrpLeu: 0.854 ± 0.015	0.231TrpMet: 0.231 ± 0.007	0.613TrpAsn: 0.613 ± 0.013	0.208TrpPro: 0.208 ± 0.006	0.253TrpGln: 0.253 ± 0.007	0.426TrpArg: 0.426 ± 0.01	0.752TrpSer: 0.752 ± 0.012	0.501TrpThr: 0.501 ± 0.01	0.451TrpVal: 0.451 ± 0.01	0.124TrpTrp: 0.124 ± 0.005	0.327TrpTyr: 0.327 ± 0.007	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.405TyrAla: 1.405 ± 0.014	0.721TyrCys: 0.721 ± 0.011	2.068TyrAsp: 2.068 ± 0.022	1.767TyrGlu: 1.767 ± 0.017	1.968TyrPhe: 1.968 ± 0.02	1.874TyrGly: 1.874 ± 0.023	1.038TyrHis: 1.038 ± 0.015	2.728TyrIle: 2.728 ± 0.023	2.134TyrLys: 2.134 ± 0.019	3.836TyrLeu: 3.836 ± 0.031	0.663TyrMet: 0.663 ± 0.011	2.738TyrAsn: 2.738 ± 0.026	1.527TyrPro: 1.527 ± 0.021	2.029TyrGln: 2.029 ± 0.022	1.517TyrArg: 1.517 ± 0.016	3.323TyrSer: 3.323 ± 0.027	2.002TyrThr: 2.002 ± 0.02	1.667TyrVal: 1.667 ± 0.019	0.341TyrTrp: 0.341 ± 0.007	1.767TyrTyr: 1.767 ± 0.021	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 12351 proteins (6384829 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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