Enterobacteria phage mEp237

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.65

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 63 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K7PJT2|K7PJT2_9CAUD Minor tail protein OS=Enterobacteria phage mEp237 OX=1147151 GN=mEp237_016 PE=4 SV=1
MM1 pKa = 7.13KK2 pKa = 10.26HH3 pKa = 5.85SDD5 pKa = 2.99IRR7 pKa = 11.84KK8 pKa = 9.17VIIDD12 pKa = 3.91ALEE15 pKa = 4.05SAIGTDD21 pKa = 3.47VIYY24 pKa = 10.73FDD26 pKa = 4.27GRR28 pKa = 11.84PAVLEE33 pKa = 4.45EE34 pKa = 4.3GDD36 pKa = 4.1FPAVAVYY43 pKa = 8.52LTDD46 pKa = 3.48AEE48 pKa = 4.64YY49 pKa = 10.08TGEE52 pKa = 4.19EE53 pKa = 4.28LDD55 pKa = 3.7ADD57 pKa = 3.63SWQAILHH64 pKa = 5.95IEE66 pKa = 4.24VFLEE70 pKa = 4.07AQVPDD75 pKa = 4.01SDD77 pKa = 5.16LDD79 pKa = 3.38DD80 pKa = 3.59WMEE83 pKa = 3.95TRR85 pKa = 11.84VYY87 pKa = 10.09PVLAEE92 pKa = 4.16VPGLEE97 pKa = 4.18SLITTMVQQGYY108 pKa = 10.04DD109 pKa = 3.56YY110 pKa = 11.36QRR112 pKa = 11.84DD113 pKa = 3.61DD114 pKa = 6.45DD115 pKa = 3.94MALWSSADD123 pKa = 3.42LKK125 pKa = 11.49YY126 pKa = 11.03SITYY130 pKa = 10.99DD131 pKa = 3.22MM132 pKa = 5.82

Molecular weight: 14.95 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K7PHF7|K7PHF7_9CAUD Uncharacterized protein OS=Enterobacteria phage mEp237 OX=1147151 GN=mEp237_034 PE=4 SV=1
MM1 pKa = 7.63RR2 pKa = 11.84LINRR6 pKa = 11.84GSKK9 pKa = 9.91QSPLARR15 pKa = 11.84QACDD19 pKa = 2.78IALAAHH25 pKa = 6.63LQTYY29 pKa = 9.95GDD31 pKa = 3.88YY32 pKa = 11.08GRR34 pKa = 11.84SKK36 pKa = 10.11MKK38 pKa = 8.96EE39 pKa = 3.88TYY41 pKa = 7.33TVKK44 pKa = 10.69VEE46 pKa = 4.31GVKK49 pKa = 10.5VWVEE53 pKa = 3.7VVNRR57 pKa = 11.84KK58 pKa = 9.34ASYY61 pKa = 10.27VATAMTGMRR70 pKa = 11.84RR71 pKa = 11.84LRR73 pKa = 11.84SLPGQVGG80 pKa = 3.11

Molecular weight: 8.9 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

63

0

63

13272

46

1151

210.7

23.34

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.69 ± 0.544

1.07 ± 0.176

5.749 ± 0.241

6.329 ± 0.407

3.255 ± 0.186

7.286 ± 0.358

1.673 ± 0.235

5.402 ± 0.233

5.267 ± 0.338

7.783 ± 0.278

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.675 ± 0.192

4.589 ± 0.232

4.106 ± 0.242

4.498 ± 0.302

5.877 ± 0.265

6.766 ± 0.267

6.412 ± 0.354

6.721 ± 0.251

1.688 ± 0.145

3.165 ± 0.163
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski