Amino acid dipepetide frequency for Enterobacteria phage mEp237

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.097AlaAla: 10.097 ± 1.246
0.904AlaCys: 0.904 ± 0.284
6.179AlaAsp: 6.179 ± 0.732
6.179AlaGlu: 6.179 ± 0.758
3.391AlaPhe: 3.391 ± 0.536
8.213AlaGly: 8.213 ± 0.715
1.808AlaHis: 1.808 ± 0.419
5.275AlaIle: 5.275 ± 0.714
4.295AlaLys: 4.295 ± 0.488
8.741AlaLeu: 8.741 ± 1.2
3.617AlaMet: 3.617 ± 0.488
3.24AlaAsn: 3.24 ± 0.454
2.035AlaPro: 2.035 ± 0.363
5.124AlaGln: 5.124 ± 0.904
5.576AlaArg: 5.576 ± 0.779
6.254AlaSer: 6.254 ± 0.716
4.898AlaThr: 4.898 ± 0.845
6.932AlaVal: 6.932 ± 0.74
1.658AlaTrp: 1.658 ± 0.308
2.487AlaTyr: 2.487 ± 0.423
0.0AlaXaa: 0.0 ± 0.0
Cys
1.055CysAla: 1.055 ± 0.278
0.151CysCys: 0.151 ± 0.111
0.754CysAsp: 0.754 ± 0.233
0.829CysGlu: 0.829 ± 0.257
0.527CysPhe: 0.527 ± 0.222
1.055CysGly: 1.055 ± 0.285
0.226CysHis: 0.226 ± 0.159
0.377CysIle: 0.377 ± 0.153
0.678CysLys: 0.678 ± 0.259
0.377CysLeu: 0.377 ± 0.17
0.226CysMet: 0.226 ± 0.155
0.377CysAsn: 0.377 ± 0.15
0.603CysPro: 0.603 ± 0.199
0.678CysGln: 0.678 ± 0.195
0.754CysArg: 0.754 ± 0.281
0.527CysSer: 0.527 ± 0.189
0.527CysThr: 0.527 ± 0.17
0.301CysVal: 0.301 ± 0.132
0.377CysTrp: 0.377 ± 0.189
0.226CysTyr: 0.226 ± 0.125
0.0CysXaa: 0.0 ± 0.0
Asp
5.727AspAla: 5.727 ± 0.553
0.829AspCys: 0.829 ± 0.267
3.994AspAsp: 3.994 ± 0.661
4.973AspGlu: 4.973 ± 0.815
1.507AspPhe: 1.507 ± 0.323
4.747AspGly: 4.747 ± 0.718
0.377AspHis: 0.377 ± 0.167
2.637AspIle: 2.637 ± 0.426
2.261AspLys: 2.261 ± 0.377
4.747AspLeu: 4.747 ± 0.708
1.432AspMet: 1.432 ± 0.342
2.788AspAsn: 2.788 ± 0.389
2.035AspPro: 2.035 ± 0.593
2.185AspGln: 2.185 ± 0.353
2.713AspArg: 2.713 ± 0.457
3.768AspSer: 3.768 ± 0.513
3.918AspThr: 3.918 ± 0.549
3.315AspVal: 3.315 ± 0.411
1.281AspTrp: 1.281 ± 0.323
2.261AspTyr: 2.261 ± 0.46
0.0AspXaa: 0.0 ± 0.0
Glu
5.35GluAla: 5.35 ± 0.626
0.754GluCys: 0.754 ± 0.3
2.261GluAsp: 2.261 ± 0.417
4.446GluGlu: 4.446 ± 0.85
1.733GluPhe: 1.733 ± 0.351
3.692GluGly: 3.692 ± 0.493
1.055GluHis: 1.055 ± 0.281
3.542GluIle: 3.542 ± 0.544
3.617GluLys: 3.617 ± 0.485
6.932GluLeu: 6.932 ± 0.851
1.733GluMet: 1.733 ± 0.341
2.939GluAsn: 2.939 ± 0.446
2.939GluPro: 2.939 ± 0.615
3.542GluGln: 3.542 ± 0.637
3.542GluArg: 3.542 ± 0.595
4.672GluSer: 4.672 ± 0.45
3.918GluThr: 3.918 ± 0.451
3.391GluVal: 3.391 ± 0.496
0.754GluTrp: 0.754 ± 0.246
2.487GluTyr: 2.487 ± 0.504
0.0GluXaa: 0.0 ± 0.0
Phe
1.959PheAla: 1.959 ± 0.419
0.603PheCys: 0.603 ± 0.184
2.185PheAsp: 2.185 ± 0.4
2.11PheGlu: 2.11 ± 0.382
1.13PhePhe: 1.13 ± 0.328
2.411PheGly: 2.411 ± 0.415
0.829PheHis: 0.829 ± 0.209
1.432PheIle: 1.432 ± 0.316
1.582PheLys: 1.582 ± 0.344
1.884PheLeu: 1.884 ± 0.331
0.829PheMet: 0.829 ± 0.295
1.808PheAsn: 1.808 ± 0.459
2.185PhePro: 2.185 ± 0.537
0.98PheGln: 0.98 ± 0.256
2.261PheArg: 2.261 ± 0.4
2.11PheSer: 2.11 ± 0.372
2.637PheThr: 2.637 ± 0.407
2.035PheVal: 2.035 ± 0.326
0.754PheTrp: 0.754 ± 0.193
0.829PheTyr: 0.829 ± 0.208
0.0PheXaa: 0.0 ± 0.0
Gly
5.501GlyAla: 5.501 ± 0.641
0.904GlyCys: 0.904 ± 0.266
3.843GlyAsp: 3.843 ± 0.464
3.617GlyGlu: 3.617 ± 0.354
2.035GlyPhe: 2.035 ± 0.29
5.877GlyGly: 5.877 ± 0.837
0.678GlyHis: 0.678 ± 0.268
4.672GlyIle: 4.672 ± 0.492
4.295GlyLys: 4.295 ± 0.492
6.028GlyLeu: 6.028 ± 0.702
3.315GlyMet: 3.315 ± 0.495
3.994GlyAsn: 3.994 ± 0.662
1.432GlyPro: 1.432 ± 0.302
3.089GlyGln: 3.089 ± 0.495
4.069GlyArg: 4.069 ± 0.605
4.295GlySer: 4.295 ± 0.578
5.35GlyThr: 5.35 ± 0.91
5.501GlyVal: 5.501 ± 0.527
1.055GlyTrp: 1.055 ± 0.318
3.315GlyTyr: 3.315 ± 0.399
0.0GlyXaa: 0.0 ± 0.0
His
1.582HisAla: 1.582 ± 0.355
0.377HisCys: 0.377 ± 0.159
1.13HisAsp: 1.13 ± 0.269
1.206HisGlu: 1.206 ± 0.339
1.13HisPhe: 1.13 ± 0.35
0.678HisGly: 0.678 ± 0.168
0.829HisHis: 0.829 ± 0.261
1.356HisIle: 1.356 ± 0.283
0.377HisLys: 0.377 ± 0.164
1.658HisLeu: 1.658 ± 0.456
0.301HisMet: 0.301 ± 0.173
0.603HisAsn: 0.603 ± 0.177
0.678HisPro: 0.678 ± 0.263
0.603HisGln: 0.603 ± 0.233
0.98HisArg: 0.98 ± 0.214
0.603HisSer: 0.603 ± 0.201
0.603HisThr: 0.603 ± 0.186
0.98HisVal: 0.98 ± 0.247
0.301HisTrp: 0.301 ± 0.159
0.754HisTyr: 0.754 ± 0.26
0.0HisXaa: 0.0 ± 0.0
Ile
4.973IleAla: 4.973 ± 0.679
0.98IleCys: 0.98 ± 0.256
3.014IleAsp: 3.014 ± 0.484
4.446IleGlu: 4.446 ± 0.703
1.582IlePhe: 1.582 ± 0.311
3.315IleGly: 3.315 ± 0.433
0.754IleHis: 0.754 ± 0.273
3.24IleIle: 3.24 ± 0.496
3.391IleLys: 3.391 ± 0.51
3.24IleLeu: 3.24 ± 0.398
0.829IleMet: 0.829 ± 0.313
3.014IleAsn: 3.014 ± 0.608
2.411IlePro: 2.411 ± 0.427
2.035IleGln: 2.035 ± 0.436
3.315IleArg: 3.315 ± 0.474
4.672IleSer: 4.672 ± 0.649
3.165IleThr: 3.165 ± 0.549
3.165IleVal: 3.165 ± 0.522
1.206IleTrp: 1.206 ± 0.303
1.281IleTyr: 1.281 ± 0.299
0.0IleXaa: 0.0 ± 0.0
Lys
4.295LysAla: 4.295 ± 0.651
0.452LysCys: 0.452 ± 0.198
2.863LysAsp: 2.863 ± 0.422
3.994LysGlu: 3.994 ± 0.612
1.13LysPhe: 1.13 ± 0.332
2.863LysGly: 2.863 ± 0.39
1.055LysHis: 1.055 ± 0.274
3.089LysIle: 3.089 ± 0.57
3.617LysLys: 3.617 ± 0.537
3.918LysLeu: 3.918 ± 0.715
0.904LysMet: 0.904 ± 0.307
2.336LysAsn: 2.336 ± 0.357
2.411LysPro: 2.411 ± 0.378
2.713LysGln: 2.713 ± 0.501
2.487LysArg: 2.487 ± 0.521
4.144LysSer: 4.144 ± 0.558
4.446LysThr: 4.446 ± 0.513
3.466LysVal: 3.466 ± 0.539
0.678LysTrp: 0.678 ± 0.276
1.808LysTyr: 1.808 ± 0.405
0.0LysXaa: 0.0 ± 0.0
Leu
7.611LeuAla: 7.611 ± 0.769
0.829LeuCys: 0.829 ± 0.26
5.802LeuAsp: 5.802 ± 0.639
4.295LeuGlu: 4.295 ± 0.899
1.658LeuPhe: 1.658 ± 0.296
4.898LeuGly: 4.898 ± 0.57
1.507LeuHis: 1.507 ± 0.321
3.617LeuIle: 3.617 ± 0.695
4.22LeuLys: 4.22 ± 0.654
5.501LeuLeu: 5.501 ± 0.963
1.658LeuMet: 1.658 ± 0.334
3.165LeuAsn: 3.165 ± 0.478
4.521LeuPro: 4.521 ± 0.673
3.843LeuGln: 3.843 ± 0.598
5.049LeuArg: 5.049 ± 0.627
6.028LeuSer: 6.028 ± 0.753
5.727LeuThr: 5.727 ± 0.661
4.747LeuVal: 4.747 ± 0.629
0.98LeuTrp: 0.98 ± 0.231
2.185LeuTyr: 2.185 ± 0.353
0.0LeuXaa: 0.0 ± 0.0
Met
3.542MetAla: 3.542 ± 0.596
0.151MetCys: 0.151 ± 0.087
1.356MetAsp: 1.356 ± 0.292
1.055MetGlu: 1.055 ± 0.282
0.678MetPhe: 0.678 ± 0.243
1.658MetGly: 1.658 ± 0.317
0.377MetHis: 0.377 ± 0.17
1.13MetIle: 1.13 ± 0.321
2.487MetLys: 2.487 ± 0.427
2.261MetLeu: 2.261 ± 0.4
0.527MetMet: 0.527 ± 0.198
0.829MetAsn: 0.829 ± 0.285
0.904MetPro: 0.904 ± 0.263
1.13MetGln: 1.13 ± 0.281
1.582MetArg: 1.582 ± 0.398
2.11MetSer: 2.11 ± 0.412
2.185MetThr: 2.185 ± 0.448
1.884MetVal: 1.884 ± 0.304
0.377MetTrp: 0.377 ± 0.186
0.527MetTyr: 0.527 ± 0.202
0.0MetXaa: 0.0 ± 0.0
Asn
4.295AsnAla: 4.295 ± 0.589
0.452AsnCys: 0.452 ± 0.204
2.185AsnAsp: 2.185 ± 0.552
3.014AsnGlu: 3.014 ± 0.511
1.432AsnPhe: 1.432 ± 0.418
4.596AsnGly: 4.596 ± 0.661
0.603AsnHis: 0.603 ± 0.225
2.788AsnIle: 2.788 ± 0.456
1.658AsnLys: 1.658 ± 0.39
2.788AsnLeu: 2.788 ± 0.438
0.98AsnMet: 0.98 ± 0.29
2.185AsnAsn: 2.185 ± 0.448
2.637AsnPro: 2.637 ± 0.382
1.582AsnGln: 1.582 ± 0.318
2.939AsnArg: 2.939 ± 0.59
3.165AsnSer: 3.165 ± 0.501
3.315AsnThr: 3.315 ± 0.621
2.487AsnVal: 2.487 ± 0.453
1.13AsnTrp: 1.13 ± 0.308
1.658AsnTyr: 1.658 ± 0.307
0.0AsnXaa: 0.0 ± 0.0
Pro
4.596ProAla: 4.596 ± 0.652
0.226ProCys: 0.226 ± 0.121
3.315ProAsp: 3.315 ± 0.531
2.939ProGlu: 2.939 ± 0.658
1.884ProPhe: 1.884 ± 0.338
4.069ProGly: 4.069 ± 0.508
0.603ProHis: 0.603 ± 0.202
1.281ProIle: 1.281 ± 0.294
2.035ProLys: 2.035 ± 0.419
2.562ProLeu: 2.562 ± 0.438
0.452ProMet: 0.452 ± 0.159
1.206ProAsn: 1.206 ± 0.261
1.808ProPro: 1.808 ± 0.344
1.733ProGln: 1.733 ± 0.39
1.808ProArg: 1.808 ± 0.488
2.562ProSer: 2.562 ± 0.509
2.261ProThr: 2.261 ± 0.446
4.144ProVal: 4.144 ± 0.633
0.829ProTrp: 0.829 ± 0.252
0.754ProTyr: 0.754 ± 0.22
0.0ProXaa: 0.0 ± 0.0
Gln
4.446GlnAla: 4.446 ± 0.811
0.301GlnCys: 0.301 ± 0.164
1.582GlnAsp: 1.582 ± 0.319
3.391GlnGlu: 3.391 ± 0.479
1.432GlnPhe: 1.432 ± 0.221
1.808GlnGly: 1.808 ± 0.385
0.452GlnHis: 0.452 ± 0.194
2.035GlnIle: 2.035 ± 0.362
2.637GlnLys: 2.637 ± 0.577
4.521GlnLeu: 4.521 ± 0.534
1.582GlnMet: 1.582 ± 0.344
2.261GlnAsn: 2.261 ± 0.405
2.185GlnPro: 2.185 ± 0.42
2.713GlnGln: 2.713 ± 0.636
2.487GlnArg: 2.487 ± 0.326
3.089GlnSer: 3.089 ± 0.534
3.089GlnThr: 3.089 ± 0.526
3.391GlnVal: 3.391 ± 0.411
0.452GlnTrp: 0.452 ± 0.168
1.13GlnTyr: 1.13 ± 0.259
0.0GlnXaa: 0.0 ± 0.0
Arg
5.199ArgAla: 5.199 ± 0.891
0.452ArgCys: 0.452 ± 0.196
2.336ArgAsp: 2.336 ± 0.445
3.089ArgGlu: 3.089 ± 0.498
2.185ArgPhe: 2.185 ± 0.34
3.542ArgGly: 3.542 ± 0.503
1.13ArgHis: 1.13 ± 0.317
3.994ArgIle: 3.994 ± 0.454
3.617ArgLys: 3.617 ± 0.457
5.199ArgLeu: 5.199 ± 0.788
1.884ArgMet: 1.884 ± 0.394
3.542ArgAsn: 3.542 ± 0.443
1.733ArgPro: 1.733 ± 0.293
2.637ArgGln: 2.637 ± 0.564
4.672ArgArg: 4.672 ± 0.826
2.788ArgSer: 2.788 ± 0.517
3.089ArgThr: 3.089 ± 0.383
4.823ArgVal: 4.823 ± 0.595
0.904ArgTrp: 0.904 ± 0.3
1.959ArgTyr: 1.959 ± 0.319
0.0ArgXaa: 0.0 ± 0.0
Ser
7.987SerAla: 7.987 ± 0.839
0.452SerCys: 0.452 ± 0.176
4.823SerAsp: 4.823 ± 0.693
3.542SerGlu: 3.542 ± 0.634
2.11SerPhe: 2.11 ± 0.336
6.33SerGly: 6.33 ± 0.811
1.281SerHis: 1.281 ± 0.294
3.617SerIle: 3.617 ± 0.594
2.863SerLys: 2.863 ± 0.593
4.596SerLeu: 4.596 ± 0.507
1.432SerMet: 1.432 ± 0.223
2.863SerAsn: 2.863 ± 0.381
2.411SerPro: 2.411 ± 0.37
2.788SerGln: 2.788 ± 0.343
3.692SerArg: 3.692 ± 0.495
4.22SerSer: 4.22 ± 0.733
4.069SerThr: 4.069 ± 0.635
5.651SerVal: 5.651 ± 0.69
1.432SerTrp: 1.432 ± 0.271
1.507SerTyr: 1.507 ± 0.343
0.0SerXaa: 0.0 ± 0.0
Thr
7.611ThrAla: 7.611 ± 1.011
0.603ThrCys: 0.603 ± 0.224
3.617ThrAsp: 3.617 ± 0.583
3.542ThrGlu: 3.542 ± 0.498
2.487ThrPhe: 2.487 ± 0.471
5.049ThrGly: 5.049 ± 0.775
1.356ThrHis: 1.356 ± 0.334
3.617ThrIle: 3.617 ± 0.492
2.185ThrLys: 2.185 ± 0.48
5.651ThrLeu: 5.651 ± 0.694
1.582ThrMet: 1.582 ± 0.367
2.336ThrAsn: 2.336 ± 0.473
3.24ThrPro: 3.24 ± 0.558
2.713ThrGln: 2.713 ± 0.38
3.542ThrArg: 3.542 ± 0.435
3.542ThrSer: 3.542 ± 0.553
3.391ThrThr: 3.391 ± 0.562
4.446ThrVal: 4.446 ± 0.692
1.206ThrTrp: 1.206 ± 0.298
2.411ThrTyr: 2.411 ± 0.47
0.0ThrXaa: 0.0 ± 0.0
Val
6.104ValAla: 6.104 ± 0.557
0.452ValCys: 0.452 ± 0.191
3.843ValAsp: 3.843 ± 0.584
3.918ValGlu: 3.918 ± 0.589
2.713ValPhe: 2.713 ± 0.454
3.768ValGly: 3.768 ± 0.596
0.829ValHis: 0.829 ± 0.248
3.994ValIle: 3.994 ± 0.663
5.049ValLys: 5.049 ± 0.671
4.069ValLeu: 4.069 ± 0.573
2.637ValMet: 2.637 ± 0.556
3.994ValAsn: 3.994 ± 0.473
2.411ValPro: 2.411 ± 0.394
2.487ValGln: 2.487 ± 0.438
3.843ValArg: 3.843 ± 0.534
6.028ValSer: 6.028 ± 0.844
4.521ValThr: 4.521 ± 0.695
3.542ValVal: 3.542 ± 0.435
0.754ValTrp: 0.754 ± 0.25
2.261ValTyr: 2.261 ± 0.468
0.0ValXaa: 0.0 ± 0.0
Trp
1.507TrpAla: 1.507 ± 0.248
0.301TrpCys: 0.301 ± 0.13
0.754TrpAsp: 0.754 ± 0.2
0.904TrpGlu: 0.904 ± 0.292
0.754TrpPhe: 0.754 ± 0.228
1.507TrpGly: 1.507 ± 0.373
0.377TrpHis: 0.377 ± 0.196
0.98TrpIle: 0.98 ± 0.256
0.678TrpLys: 0.678 ± 0.198
1.432TrpLeu: 1.432 ± 0.307
0.301TrpMet: 0.301 ± 0.135
0.754TrpAsn: 0.754 ± 0.243
0.678TrpPro: 0.678 ± 0.216
0.98TrpGln: 0.98 ± 0.275
0.904TrpArg: 0.904 ± 0.231
0.904TrpSer: 0.904 ± 0.196
0.98TrpThr: 0.98 ± 0.36
1.281TrpVal: 1.281 ± 0.335
0.226TrpTrp: 0.226 ± 0.136
0.678TrpTyr: 0.678 ± 0.209
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.466TyrAla: 3.466 ± 0.451
0.527TyrCys: 0.527 ± 0.205
1.658TyrAsp: 1.658 ± 0.352
1.808TyrGlu: 1.808 ± 0.326
1.055TyrPhe: 1.055 ± 0.29
2.788TyrGly: 2.788 ± 0.6
0.603TyrHis: 0.603 ± 0.263
1.356TyrIle: 1.356 ± 0.311
1.13TyrLys: 1.13 ± 0.299
1.733TyrLeu: 1.733 ± 0.497
0.452TyrMet: 0.452 ± 0.169
1.658TyrAsn: 1.658 ± 0.363
1.808TyrPro: 1.808 ± 0.351
1.432TyrGln: 1.432 ± 0.307
2.562TyrArg: 2.562 ± 0.536
2.185TyrSer: 2.185 ± 0.615
2.035TyrThr: 2.035 ± 0.378
1.733TyrVal: 1.733 ± 0.388
0.527TyrTrp: 0.527 ± 0.2
1.13TyrTyr: 1.13 ± 0.264
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 63 proteins (13272 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski