Gloeobacter kilaueensis JS1

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Gloeobacteria; Gloeobacterales; Gloeobacteraceae; Gloeobacter; Gloeobacter kilaueensis

Average proteome isoelectric point is 6.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4462 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|U5QJF8|U5QJF8_9CYAN Cytochrome b6-f complex subunit 4 OS=Gloeobacter kilaueensis JS1 OX=1183438 GN=petD PE=3 SV=1
MM1 pKa = 7.61ISKK4 pKa = 6.76TTLAALVSAGLLLPLLGSSAGAYY27 pKa = 8.81TYY29 pKa = 11.07VEE31 pKa = 4.08QGGDD35 pKa = 3.22AGEE38 pKa = 4.43TPTTFQNIAIGAPGLTGGAGSFDD61 pKa = 3.17IQGNISEE68 pKa = 4.53IDD70 pKa = 3.43QVSTGFADD78 pKa = 3.57ADD80 pKa = 3.99VYY82 pKa = 11.48AVALTAGQAFTATVTATSTTQPNGVPTADD111 pKa = 3.43TDD113 pKa = 4.47LVLLNSQGSGLAFNANIFVDD133 pKa = 4.17PNDD136 pKa = 3.86PFNNNLNSQLTFTPTVSGTYY156 pKa = 10.08YY157 pKa = 10.78LGISLAGYY165 pKa = 10.27DD166 pKa = 4.56PINFASGSGVFDD178 pKa = 4.43PSLTPATGTYY188 pKa = 9.63QPPQPTPLWDD198 pKa = 3.6SYY200 pKa = 11.85FEE202 pKa = 4.79LGSIAPDD209 pKa = 3.53TFAYY213 pKa = 10.8NIDD216 pKa = 3.95LQGATPTPEE225 pKa = 3.89PTVGGGFIAVGALGLVAGRR244 pKa = 11.84RR245 pKa = 11.84RR246 pKa = 11.84KK247 pKa = 9.8ARR249 pKa = 11.84AKK251 pKa = 8.67QTSS254 pKa = 3.01

Molecular weight: 25.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|U5QBP7|U5QBP7_9CYAN LysR family transcriptional regulator OS=Gloeobacter kilaueensis JS1 OX=1183438 GN=GKIL_0034 PE=3 SV=1
MM1 pKa = 7.68RR2 pKa = 11.84RR3 pKa = 11.84TLEE6 pKa = 3.64GTTRR10 pKa = 11.84KK11 pKa = 9.32RR12 pKa = 11.84QKK14 pKa = 9.42TSGFRR19 pKa = 11.84ARR21 pKa = 11.84MRR23 pKa = 11.84TPGGRR28 pKa = 11.84RR29 pKa = 11.84VLSARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84GRR39 pKa = 11.84HH40 pKa = 4.72RR41 pKa = 11.84LAII44 pKa = 4.09

Molecular weight: 5.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4462

0

4462

1407085

27

2796

315.3

34.59

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.933 ± 0.055

0.967 ± 0.014

4.973 ± 0.034

5.882 ± 0.045

3.725 ± 0.023

7.876 ± 0.038

1.818 ± 0.018

4.801 ± 0.029

2.917 ± 0.032

11.819 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.511 ± 0.013

2.892 ± 0.027

5.557 ± 0.036

4.68 ± 0.028

7.073 ± 0.038

5.962 ± 0.031

5.216 ± 0.03

7.167 ± 0.03

1.493 ± 0.018

2.736 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski