Arenitalea lutea
Average proteome isoelectric point is 6.52
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2965 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1M6ANY2|A0A1M6ANY2_9FLAO Predicted DNA-binding protein MmcQ/YjbR family OS=Arenitalea lutea OX=1178825 GN=SAMN05216261_0536 PE=4 SV=1
MM1 pKa = 7.54 KK2 pKa = 10.21 NIKK5 pKa = 9.67 ILSLLLLAIIGFNACDD21 pKa = 3.5 QDD23 pKa = 4.08 DD24 pKa = 4.53 DD25 pKa = 5.15 LVFTAQEE32 pKa = 4.04 PTDD35 pKa = 4.55 GIVFSNSFLDD45 pKa = 4.13 EE46 pKa = 4.24 YY47 pKa = 10.96 VLTVAASNNLGEE59 pKa = 4.22 RR60 pKa = 11.84 FTWKK64 pKa = 10.18 DD65 pKa = 3.16 ANFGVPTNVTYY76 pKa = 10.5 EE77 pKa = 4.26 LEE79 pKa = 3.94 NSIIGDD85 pKa = 3.8 FTDD88 pKa = 3.29 VTTVGTTDD96 pKa = 3.31 GNEE99 pKa = 3.83 LAVTIGKK106 pKa = 8.61 MLEE109 pKa = 3.85 FATAAGLDD117 pKa = 3.74 NDD119 pKa = 5.08 PNTDD123 pKa = 3.64 LPNTGTLYY131 pKa = 10.69 FRR133 pKa = 11.84 VKK135 pKa = 10.81 GFIGTEE141 pKa = 3.98 GLPTYY146 pKa = 10.92 SPVQEE151 pKa = 4.21 LTVVLPEE158 pKa = 3.83 IVEE161 pKa = 4.19 GGGAFEE167 pKa = 3.9 IASWGVVGSGYY178 pKa = 10.66 NSWGAFADD186 pKa = 3.99 GKK188 pKa = 10.81 FYY190 pKa = 8.5 TTAVPGVIVSYY201 pKa = 11.41 VNLVDD206 pKa = 4.49 GEE208 pKa = 4.4 IKK210 pKa = 10.4 FRR212 pKa = 11.84 EE213 pKa = 4.0 NNQWGGDD220 pKa = 3.59 LGDD223 pKa = 4.23 ANGDD227 pKa = 3.73 GVLDD231 pKa = 4.7 ADD233 pKa = 4.52 PDD235 pKa = 3.82 NNIAVTAGDD244 pKa = 3.74 YY245 pKa = 10.84 KK246 pKa = 10.35 ITINTNDD253 pKa = 3.2 NSYY256 pKa = 9.88 TIEE259 pKa = 4.22 PFSWGIVGSGYY270 pKa = 10.55 NDD272 pKa = 3.2 WGGAGPDD279 pKa = 2.97 AKK281 pKa = 10.44 LYY283 pKa = 10.52 YY284 pKa = 10.68 DD285 pKa = 3.61 HH286 pKa = 6.89 TTDD289 pKa = 3.14 TFKK292 pKa = 11.55 ANVKK296 pKa = 10.19 LLTGEE301 pKa = 3.93 IKK303 pKa = 10.54 FRR305 pKa = 11.84 MNNSWGGDD313 pKa = 3.33 LGDD316 pKa = 4.27 ANGDD320 pKa = 3.74 GVLDD324 pKa = 4.26 ADD326 pKa = 4.56 ADD328 pKa = 4.12 NNIATTEE335 pKa = 3.84 GHH337 pKa = 5.9 YY338 pKa = 10.92 VVTVNLNDD346 pKa = 3.26 NSYY349 pKa = 10.92 SIVEE353 pKa = 4.17 GTVWGAVGSGYY364 pKa = 10.54 NDD366 pKa = 3.18 WGGAGPDD373 pKa = 3.34 AALTEE378 pKa = 4.19 IQPGVWYY385 pKa = 10.36 AEE387 pKa = 3.91 NVTLVDD393 pKa = 3.7 GEE395 pKa = 4.7 LKK397 pKa = 10.39 FRR399 pKa = 11.84 PNNTWSGDD407 pKa = 3.61 YY408 pKa = 11.23 GDD410 pKa = 5.56 ANGDD414 pKa = 3.82 NILDD418 pKa = 3.51 QDD420 pKa = 3.96 ADD422 pKa = 3.71 NNIAVTAGNYY432 pKa = 9.57 VISIDD437 pKa = 3.99 FNDD440 pKa = 4.2 PAGPAYY446 pKa = 10.73 YY447 pKa = 10.32 LGSRR451 pKa = 4.06
Molecular weight: 48.1 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.685
IPC2_protein 3.643
IPC_protein 3.681
Toseland 3.452
ProMoST 3.859
Dawson 3.694
Bjellqvist 3.846
Wikipedia 3.63
Rodwell 3.503
Grimsley 3.363
Solomon 3.681
Lehninger 3.643
Nozaki 3.795
DTASelect 4.075
Thurlkill 3.503
EMBOSS 3.643
Sillero 3.808
Patrickios 1.303
IPC_peptide 3.681
IPC2_peptide 3.783
IPC2.peptide.svr19 3.74
Protein with the highest isoelectric point:
>tr|A0A1M6AXR0|A0A1M6AXR0_9FLAO Predicted oxidoreductase OS=Arenitalea lutea OX=1178825 GN=SAMN05216261_0678 PE=4 SV=1
MM1 pKa = 7.84 PSGKK5 pKa = 9.32 KK6 pKa = 9.59 RR7 pKa = 11.84 KK8 pKa = 7.05 RR9 pKa = 11.84 HH10 pKa = 5.11 KK11 pKa = 10.59 VATHH15 pKa = 5.21 KK16 pKa = 10.3 RR17 pKa = 11.84 KK18 pKa = 9.62 KK19 pKa = 9.04 RR20 pKa = 11.84 RR21 pKa = 11.84 RR22 pKa = 11.84 ANRR25 pKa = 11.84 HH26 pKa = 4.77 KK27 pKa = 10.58 KK28 pKa = 9.8 KK29 pKa = 10.51 KK30 pKa = 10.04
Molecular weight: 3.76 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.473
IPC2_protein 11.082
IPC_protein 12.618
Toseland 12.808
ProMoST 13.276
Dawson 12.808
Bjellqvist 12.793
Wikipedia 13.276
Rodwell 12.735
Grimsley 12.837
Solomon 13.29
Lehninger 13.188
Nozaki 12.808
DTASelect 12.793
Thurlkill 12.808
EMBOSS 13.29
Sillero 12.808
Patrickios 12.457
IPC_peptide 13.29
IPC2_peptide 12.266
IPC2.peptide.svr19 9.03
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2965
0
2965
1027488
29
2900
346.5
39.08
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.31 ± 0.044
0.791 ± 0.015
5.798 ± 0.045
6.5 ± 0.041
5.259 ± 0.035
6.339 ± 0.046
1.81 ± 0.022
8.158 ± 0.043
7.864 ± 0.061
9.184 ± 0.051
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.099 ± 0.024
6.52 ± 0.051
3.32 ± 0.027
3.232 ± 0.025
3.222 ± 0.028
6.471 ± 0.034
5.789 ± 0.047
6.257 ± 0.029
1.051 ± 0.017
4.025 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here