Pseudaminobacter salicylatoxidans
Average proteome isoelectric point is 6.52
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4695 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A316C148|A0A316C148_PSESE Ornithine carbamoyltransferase OS=Pseudaminobacter salicylatoxidans OX=93369 GN=C7441_110165 PE=3 SV=1
MM1 pKa = 8.01 RR2 pKa = 11.84 YY3 pKa = 9.56 SFCHH7 pKa = 6.42 TIHH10 pKa = 7.59 DD11 pKa = 4.11 EE12 pKa = 4.04 ALGIDD17 pKa = 4.11 VIYY20 pKa = 10.64 DD21 pKa = 3.48 IEE23 pKa = 4.69 CEE25 pKa = 3.92 IEE27 pKa = 4.3 LSVVPDD33 pKa = 4.05 AGAPQVSVDD42 pKa = 3.24 GVYY45 pKa = 10.81 VDD47 pKa = 4.57 GKK49 pKa = 10.67 NLFAGSAISKK59 pKa = 10.39 AIAAEE64 pKa = 3.97 IANAAVDD71 pKa = 5.09 DD72 pKa = 5.03 DD73 pKa = 5.23 DD74 pKa = 3.95 LTARR78 pKa = 11.84 AIEE81 pKa = 4.47 DD82 pKa = 3.12 EE83 pKa = 4.43 GFVYY87 pKa = 10.37 RR88 pKa = 11.84 GLGANDD94 pKa = 3.58 PDD96 pKa = 3.4 GRR98 pKa = 11.84 YY99 pKa = 8.53 VHH101 pKa = 6.7 VSS103 pKa = 2.93
Molecular weight: 10.98 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.785
IPC2_protein 3.999
IPC_protein 3.961
Toseland 3.757
ProMoST 4.126
Dawson 3.961
Bjellqvist 4.113
Wikipedia 3.897
Rodwell 3.795
Grimsley 3.668
Solomon 3.948
Lehninger 3.91
Nozaki 4.075
DTASelect 4.317
Thurlkill 3.808
EMBOSS 3.91
Sillero 4.088
Patrickios 1.939
IPC_peptide 3.948
IPC2_peptide 4.062
IPC2.peptide.svr19 3.974
Protein with the highest isoelectric point:
>tr|A0A316BZT8|A0A316BZT8_PSESE Uncharacterized protein OS=Pseudaminobacter salicylatoxidans OX=93369 GN=C7441_112138 PE=4 SV=1
MM1 pKa = 7.92 RR2 pKa = 11.84 RR3 pKa = 11.84 GLKK6 pKa = 10.1 DD7 pKa = 3.76 DD8 pKa = 4.02 FLAYY12 pKa = 10.09 VKK14 pKa = 10.52 RR15 pKa = 11.84 NPHH18 pKa = 5.67 ANTRR22 pKa = 11.84 EE23 pKa = 3.45 IAAALDD29 pKa = 3.39 CHH31 pKa = 5.85 EE32 pKa = 4.91 AYY34 pKa = 10.54 VRR36 pKa = 11.84 STARR40 pKa = 11.84 RR41 pKa = 11.84 QGIRR45 pKa = 11.84 LASNGRR51 pKa = 11.84 RR52 pKa = 11.84 GRR54 pKa = 11.84 PVAAAPKK61 pKa = 9.86 FSRR64 pKa = 11.84 PRR66 pKa = 11.84 LRR68 pKa = 11.84 IKK70 pKa = 9.99 EE71 pKa = 3.87 FRR73 pKa = 11.84 GTPEE77 pKa = 3.9 QYY79 pKa = 10.34 DD80 pKa = 3.37 ALARR84 pKa = 11.84 KK85 pKa = 8.68 VALVDD90 pKa = 4.28 LEE92 pKa = 5.05 HH93 pKa = 7.34 RR94 pKa = 11.84 DD95 pKa = 3.91 CRR97 pKa = 11.84 WPVGDD102 pKa = 5.11 PRR104 pKa = 11.84 DD105 pKa = 3.72 TDD107 pKa = 4.14 FGFCGHH113 pKa = 6.53 IKK115 pKa = 10.47 EE116 pKa = 4.62 PGKK119 pKa = 9.73 PYY121 pKa = 9.97 CAHH124 pKa = 5.86 HH125 pKa = 5.53 QRR127 pKa = 11.84 RR128 pKa = 11.84 SEE130 pKa = 4.12 GGGTPAGRR138 pKa = 11.84 SAHH141 pKa = 6.32 RR142 pKa = 11.84 VQAA145 pKa = 3.9
Molecular weight: 16.37 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.352
IPC2_protein 9.575
IPC_protein 10.511
Toseland 10.657
ProMoST 10.423
Dawson 10.76
Bjellqvist 10.511
Wikipedia 10.994
Rodwell 10.862
Grimsley 10.804
Solomon 10.906
Lehninger 10.862
Nozaki 10.672
DTASelect 10.496
Thurlkill 10.672
EMBOSS 11.067
Sillero 10.701
Patrickios 10.584
IPC_peptide 10.906
IPC2_peptide 9.736
IPC2.peptide.svr19 8.598
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4695
0
4695
1431895
29
3888
305.0
33.16
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.238 ± 0.05
0.799 ± 0.011
5.626 ± 0.033
5.927 ± 0.033
3.862 ± 0.024
8.583 ± 0.046
2.029 ± 0.017
5.447 ± 0.028
3.523 ± 0.027
9.829 ± 0.038
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.645 ± 0.016
2.722 ± 0.02
4.984 ± 0.026
3.035 ± 0.022
7.009 ± 0.039
5.438 ± 0.025
5.279 ± 0.025
7.443 ± 0.031
1.312 ± 0.015
2.27 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here