Schistosoma mattheei
Average proteome isoelectric point is 6.85
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 22244 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A183PXU8|A0A183PXU8_9TREM Uncharacterized protein OS=Schistosoma mattheei OX=31246 GN=SMTD_LOCUS19184 PE=4 SV=1
MM1 pKa = 7.15 KK2 pKa = 10.33 ASYY5 pKa = 10.75 RR6 pKa = 11.84 NLSKK10 pKa = 10.7 FYY12 pKa = 10.88 LSEE15 pKa = 4.07 NYY17 pKa = 9.75 NEE19 pKa = 4.04 KK20 pKa = 10.38 FYY22 pKa = 10.6 PHH24 pKa = 6.93 FNSLEE29 pKa = 4.36 CIYY32 pKa = 10.82 HH33 pKa = 6.65 PEE35 pKa = 4.12 FPDD38 pKa = 3.32 LVDD41 pKa = 4.45 IVEE44 pKa = 4.79 EE45 pKa = 5.42 DD46 pKa = 5.33 DD47 pKa = 6.63 DD48 pKa = 7.45 DD49 pKa = 7.67 DD50 pKa = 7.7 DD51 pKa = 7.66 DD52 pKa = 7.68 DD53 pKa = 7.42 DD54 pKa = 7.55 DD55 pKa = 7.56 DD56 pKa = 7.7 DD57 pKa = 5.75 IDD59 pKa = 4.64 EE60 pKa = 5.67 DD61 pKa = 5.33 DD62 pKa = 5.72 DD63 pKa = 5.08 VDD65 pKa = 4.75 IDD67 pKa = 4.37 GDD69 pKa = 3.88 DD70 pKa = 5.05 DD71 pKa = 5.04 EE72 pKa = 7.03 VDD74 pKa = 3.55 EE75 pKa = 5.57 QDD77 pKa = 4.85 NNNNNNNNDD86 pKa = 4.26 RR87 pKa = 11.84 IKK89 pKa = 11.07 DD90 pKa = 3.95 AITSSNVNCDD100 pKa = 3.48 PRR102 pKa = 11.84 SVNCPCYY109 pKa = 10.72 SNDD112 pKa = 3.26 NIEE115 pKa = 4.02 YY116 pKa = 10.82 DD117 pKa = 4.8 EE118 pKa = 5.64 IPDD121 pKa = 3.76 IQDD124 pKa = 2.95 KK125 pKa = 11.14 SITFQEE131 pKa = 4.79 GNDD134 pKa = 3.6 GVTWDD139 pKa = 3.61 MKK141 pKa = 10.79 FNQSEE146 pKa = 4.24 NILIKK151 pKa = 10.53 EE152 pKa = 4.01 VTLCRR157 pKa = 11.84 KK158 pKa = 10.16 KK159 pKa = 10.35 PDD161 pKa = 3.47 EE162 pKa = 4.33 KK163 pKa = 11.03 FGLTLCYY170 pKa = 10.14 RR171 pKa = 11.84 QGDD174 pKa = 4.07 TCNTSCNVYY183 pKa = 10.42 VGEE186 pKa = 4.26
Molecular weight: 21.57 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.683
IPC2_protein 3.668
IPC_protein 3.706
Toseland 3.465
ProMoST 3.859
Dawson 3.706
Bjellqvist 3.884
Wikipedia 3.656
Rodwell 3.516
Grimsley 3.376
Solomon 3.694
Lehninger 3.656
Nozaki 3.808
DTASelect 4.088
Thurlkill 3.528
EMBOSS 3.668
Sillero 3.821
Patrickios 1.227
IPC_peptide 3.694
IPC2_peptide 3.795
IPC2.peptide.svr19 3.751
Protein with the highest isoelectric point:
>tr|A0A183PH97|A0A183PH97_9TREM Uncharacterized protein OS=Schistosoma mattheei OX=31246 GN=SMTD_LOCUS13734 PE=4 SV=1
MM1 pKa = 8.02 RR2 pKa = 11.84 LHH4 pKa = 5.18 QLRR7 pKa = 11.84 WLGHH11 pKa = 4.94 VLLMPNHH18 pKa = 6.5 RR19 pKa = 11.84 LLRR22 pKa = 11.84 RR23 pKa = 11.84 AMFSGVRR30 pKa = 11.84 VGWKK34 pKa = 9.39 KK35 pKa = 10.99 ASGGQTKK42 pKa = 7.77 TT43 pKa = 3.3
Molecular weight: 5.03 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.443
IPC2_protein 10.95
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.398
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.149
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.09
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
22244
0
22244
5383249
29
4483
242.0
27.32
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.479 ± 0.02
2.129 ± 0.011
5.235 ± 0.016
5.449 ± 0.018
3.843 ± 0.014
4.359 ± 0.023
2.93 ± 0.011
6.638 ± 0.021
5.664 ± 0.019
9.657 ± 0.025
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.151 ± 0.009
7.129 ± 0.029
4.514 ± 0.019
4.313 ± 0.016
4.959 ± 0.017
10.133 ± 0.035
6.508 ± 0.059
5.368 ± 0.017
1.014 ± 0.007
3.234 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here