Ammoniphilus sp. CFH 90114

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Aneurinibacillus group; Ammoniphilus; unclassified Ammoniphilus

Average proteome isoelectric point is 6.38

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5120 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Q1T8N8|A0A4Q1T8N8_9BACL Stage II sporulation protein M OS=Ammoniphilus sp. CFH 90114 OX=2493665 GN=spoIIM PE=4 SV=1
MM1 pKa = 7.68DD2 pKa = 3.41QHH4 pKa = 7.06IHH6 pKa = 5.67TNFTSTTEE14 pKa = 3.75AEE16 pKa = 4.08RR17 pKa = 11.84AKK19 pKa = 10.44EE20 pKa = 3.96YY21 pKa = 10.77LVSAGFNQVTIDD33 pKa = 3.64GTQMKK38 pKa = 10.45VMTNEE43 pKa = 4.06EE44 pKa = 3.73NWIAAYY50 pKa = 9.68EE51 pKa = 4.0ILNGLGGSFQEE62 pKa = 4.95GEE64 pKa = 4.29SMPPGFMEE72 pKa = 5.05HH73 pKa = 6.01YY74 pKa = 9.83QLEE77 pKa = 4.63GDD79 pKa = 4.47GEE81 pKa = 4.3EE82 pKa = 4.57GDD84 pKa = 4.14PGQEE88 pKa = 3.34VDD90 pKa = 4.48MVDD93 pKa = 3.16ADD95 pKa = 4.19RR96 pKa = 11.84YY97 pKa = 10.1IDD99 pKa = 3.52INLGYY104 pKa = 10.69GDD106 pKa = 4.29FEE108 pKa = 5.63IIEE111 pKa = 5.15PYY113 pKa = 9.29TLHH116 pKa = 7.77DD117 pKa = 4.28YY118 pKa = 11.52NKK120 pKa = 9.02LTQDD124 pKa = 3.0

Molecular weight: 14.02 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Q1T1U3|A0A4Q1T1U3_9BACL Aspartyl-phosphate phosphatase Spo0E family protein OS=Ammoniphilus sp. CFH 90114 OX=2493665 GN=EIZ39_09935 PE=4 SV=1
MM1 pKa = 7.59KK2 pKa = 8.8PTFSPNNRR10 pKa = 11.84KK11 pKa = 9.29RR12 pKa = 11.84KK13 pKa = 8.74KK14 pKa = 8.63VHH16 pKa = 5.57GFRR19 pKa = 11.84KK20 pKa = 10.01RR21 pKa = 11.84MSTKK25 pKa = 10.18NGRR28 pKa = 11.84KK29 pKa = 8.91VLAARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.81GRR39 pKa = 11.84KK40 pKa = 8.75VLSAA44 pKa = 4.05

Molecular weight: 5.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5120

0

5120

1503553

23

3174

293.7

32.94

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.056 ± 0.037

0.775 ± 0.01

4.862 ± 0.027

7.288 ± 0.044

4.231 ± 0.03

7.148 ± 0.035

2.201 ± 0.018

7.394 ± 0.032

6.147 ± 0.032

10.146 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.923 ± 0.018

3.831 ± 0.024

3.972 ± 0.024

4.16 ± 0.028

4.651 ± 0.029

6.061 ± 0.029

5.317 ± 0.025

7.29 ± 0.026

1.147 ± 0.015

3.4 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski