Apple chlorotic leaf spot virus (isolate apple) (ACLSV)

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Tymovirales; Betaflexiviridae; Trivirinae; Trichovirus; Apple chlorotic leaf spot virus

Average proteome isoelectric point is 6.93

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|P54891|RDRP_ACLSA RNA-directed RNA polymerase OS=Apple chlorotic leaf spot virus (isolate apple) OX=73472 PE=4 SV=1
MM1 pKa = 7.42AAVLNLQLKK10 pKa = 9.93VDD12 pKa = 4.05ADD14 pKa = 4.15LKK16 pKa = 11.28AFLAAEE22 pKa = 4.21GRR24 pKa = 11.84PLHH27 pKa = 6.35GKK29 pKa = 7.75TGAILEE35 pKa = 4.3QTLEE39 pKa = 4.37AIFANIAIQGTSEE52 pKa = 3.75QTEE55 pKa = 4.1FLDD58 pKa = 3.45VLVEE62 pKa = 4.23VKK64 pKa = 10.81SMEE67 pKa = 4.09DD68 pKa = 3.16QKK70 pKa = 11.74VVGSFNLKK78 pKa = 9.94EE79 pKa = 3.97VVGLIKK85 pKa = 10.26IFRR88 pKa = 11.84TTSSDD93 pKa = 3.56PNISSMTFRR102 pKa = 11.84QVCEE106 pKa = 3.82AFAPEE111 pKa = 4.08ARR113 pKa = 11.84NGLVKK118 pKa = 10.76LKK120 pKa = 10.82YY121 pKa = 10.13KK122 pKa = 10.81GVFTNLFSTTPEE134 pKa = 3.9VGGKK138 pKa = 9.94YY139 pKa = 9.57PEE141 pKa = 5.03LMFDD145 pKa = 4.06FNKK148 pKa = 9.68GLNMFIMNKK157 pKa = 9.41AQQKK161 pKa = 10.48VITNMNRR168 pKa = 11.84RR169 pKa = 11.84LLQTEE174 pKa = 4.41FAKK177 pKa = 10.83SEE179 pKa = 4.15NEE181 pKa = 3.7AKK183 pKa = 9.72MSSVTTDD190 pKa = 3.07LCVV193 pKa = 2.87

Molecular weight: 21.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P54892|MP_ACLSA Putative movement protein OS=Apple chlorotic leaf spot virus (isolate apple) OX=73472 PE=3 SV=1
MM1 pKa = 7.72MIRR4 pKa = 11.84GHH6 pKa = 5.93KK7 pKa = 10.14LKK9 pKa = 10.23IAEE12 pKa = 4.25GDD14 pKa = 3.48IPIAGVKK21 pKa = 9.81SSRR24 pKa = 11.84IYY26 pKa = 11.03SDD28 pKa = 3.02VSPFRR33 pKa = 11.84KK34 pKa = 10.12ASDD37 pKa = 4.92LMIHH41 pKa = 5.83WNEE44 pKa = 3.84FVFKK48 pKa = 10.88VMPEE52 pKa = 4.42DD53 pKa = 3.29IAGDD57 pKa = 4.15GFRR60 pKa = 11.84LASIPVIPSSEE71 pKa = 4.05VQAVLRR77 pKa = 11.84KK78 pKa = 9.69RR79 pKa = 11.84EE80 pKa = 4.1STNYY84 pKa = 7.76VHH86 pKa = 7.3WGALSISIDD95 pKa = 3.16ALFRR99 pKa = 11.84KK100 pKa = 9.49NAGVSGWCYY109 pKa = 10.45VYY111 pKa = 11.0DD112 pKa = 3.98NRR114 pKa = 11.84WEE116 pKa = 4.18TFEE119 pKa = 4.38QAMLQKK125 pKa = 10.47FRR127 pKa = 11.84FNLDD131 pKa = 3.1SGSATLVTSPNFPVSLDD148 pKa = 3.76DD149 pKa = 4.79PGLSNSISVAVMFEE163 pKa = 3.79NLNFKK168 pKa = 10.66LEE170 pKa = 4.24SYY172 pKa = 9.46PISVRR177 pKa = 11.84VGNMCRR183 pKa = 11.84FFDD186 pKa = 4.65SFLSCVKK193 pKa = 10.78NKK195 pKa = 9.87VDD197 pKa = 5.0SNFLLEE203 pKa = 4.68AANADD208 pKa = 3.96PLGAGAFGFEE218 pKa = 4.23QDD220 pKa = 4.16DD221 pKa = 3.85QVSEE225 pKa = 4.25LFNYY229 pKa = 8.89IQTVPTQAIKK239 pKa = 10.65SRR241 pKa = 11.84EE242 pKa = 4.09HH243 pKa = 5.44EE244 pKa = 4.27VPRR247 pKa = 11.84GLFGMMGKK255 pKa = 9.96KK256 pKa = 9.39KK257 pKa = 10.4VKK259 pKa = 10.24SFEE262 pKa = 4.07FASGSRR268 pKa = 11.84NLGRR272 pKa = 11.84RR273 pKa = 11.84KK274 pKa = 8.91PQRR277 pKa = 11.84GRR279 pKa = 11.84PLEE282 pKa = 4.14RR283 pKa = 11.84SASLRR288 pKa = 11.84VAPGFRR294 pKa = 11.84SQDD297 pKa = 2.94EE298 pKa = 4.4RR299 pKa = 11.84VEE301 pKa = 4.13HH302 pKa = 6.25QGLSTDD308 pKa = 3.31SDD310 pKa = 3.95FEE312 pKa = 4.18NFLRR316 pKa = 11.84KK317 pKa = 9.94GKK319 pKa = 10.4GKK321 pKa = 10.5AGTEE325 pKa = 4.55SITSEE330 pKa = 3.89GSSFDD335 pKa = 3.91NISARR340 pKa = 11.84EE341 pKa = 3.87FQFARR346 pKa = 11.84QDD348 pKa = 3.42QKK350 pKa = 12.0AKK352 pKa = 10.64DD353 pKa = 3.82GGSAEE358 pKa = 4.32PPIKK362 pKa = 10.14SGRR365 pKa = 11.84RR366 pKa = 11.84SEE368 pKa = 4.39SVPGRR373 pKa = 11.84RR374 pKa = 11.84RR375 pKa = 11.84QTPSWKK381 pKa = 10.16DD382 pKa = 3.22RR383 pKa = 11.84GNSGTDD389 pKa = 2.9TGGHH393 pKa = 5.69LRR395 pKa = 11.84EE396 pKa = 4.7HH397 pKa = 7.27SDD399 pKa = 3.41TGDD402 pKa = 3.25LGTNRR407 pKa = 11.84VPGRR411 pKa = 11.84AGGGEE416 pKa = 3.57IHH418 pKa = 6.72GGSEE422 pKa = 4.15GGGVIQSEE430 pKa = 4.44GGSRR434 pKa = 11.84FDD436 pKa = 5.53QNIQDD441 pKa = 4.16YY442 pKa = 11.0IFGPEE447 pKa = 4.13YY448 pKa = 9.96KK449 pKa = 10.09QHH451 pKa = 7.13DD452 pKa = 4.65LPPSVV457 pKa = 3.85

Molecular weight: 50.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

2535

193

1885

845.0

96.12

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.207 ± 0.727

2.091 ± 0.655

5.641 ± 0.314

7.495 ± 0.407

5.838 ± 0.306

6.943 ± 1.234

2.367 ± 0.43

5.957 ± 0.583

7.89 ± 0.871

8.679 ± 0.921

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.761 ± 0.274

4.024 ± 0.478

3.708 ± 0.43

3.274 ± 0.434

6.114 ± 0.557

8.481 ± 0.794

3.708 ± 0.559

5.838 ± 0.507

1.065 ± 0.187

2.919 ± 0.677
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski