Fructilactobacillus fructivorans
Average proteome isoelectric point is 6.78
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1281 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0C1LZ35|A0A0C1LZ35_9LACO Putative ribosomal protein OS=Fructilactobacillus fructivorans OX=1614 GN=LF543_03025 PE=4 SV=1
MM1 pKa = 7.87 IYY3 pKa = 11.02 GEE5 pKa = 4.1 FASFYY10 pKa = 11.2 DD11 pKa = 3.57 EE12 pKa = 5.88 LFDD15 pKa = 4.74 NEE17 pKa = 5.87 LYY19 pKa = 10.82 KK20 pKa = 10.51 KK21 pKa = 8.4 WCNFVVSNVPKK32 pKa = 10.27 SARR35 pKa = 11.84 ILDD38 pKa = 4.35 LACGTGRR45 pKa = 11.84 LLVQLKK51 pKa = 9.68 NAGYY55 pKa = 9.43 QVAGADD61 pKa = 4.49 LSDD64 pKa = 4.91 DD65 pKa = 3.63 MLAIANDD72 pKa = 3.84 HH73 pKa = 6.46 LNEE76 pKa = 4.08 NGIFDD81 pKa = 4.4 VEE83 pKa = 4.51 LINANMLDD91 pKa = 4.08 LDD93 pKa = 4.33 GLPKK97 pKa = 10.6 YY98 pKa = 10.54 GAITCFDD105 pKa = 4.19 DD106 pKa = 5.15 SICYY110 pKa = 9.03 LANIDD115 pKa = 4.38 QVMQMFAQVRR125 pKa = 11.84 NHH127 pKa = 6.48 LSSNGKK133 pKa = 9.36 FLFDD137 pKa = 4.96 VITPYY142 pKa = 9.29 QTDD145 pKa = 3.03 IVYY148 pKa = 9.86 PGYY151 pKa = 10.06 MFNTNDD157 pKa = 3.38 GDD159 pKa = 4.53 HH160 pKa = 6.83 AFMWNTFIGKK170 pKa = 8.85 VPHH173 pKa = 6.39 SVEE176 pKa = 4.05 HH177 pKa = 6.72 DD178 pKa = 3.49 LSFFDD183 pKa = 4.93 YY184 pKa = 10.87 NHH186 pKa = 6.63 EE187 pKa = 4.03 LDD189 pKa = 5.79 AFDD192 pKa = 5.41 EE193 pKa = 4.43 YY194 pKa = 11.79 NEE196 pKa = 4.18 THH198 pKa = 7.19 KK199 pKa = 10.96 EE200 pKa = 3.65 RR201 pKa = 11.84 TYY203 pKa = 11.69 SLVEE207 pKa = 3.83 YY208 pKa = 10.73 QDD210 pKa = 3.8 ALKK213 pKa = 10.86 SAGFNNVRR221 pKa = 11.84 VSSDD225 pKa = 3.28 FGNDD229 pKa = 2.95 EE230 pKa = 4.03 VHH232 pKa = 7.86 DD233 pKa = 4.09 DD234 pKa = 3.38 TTRR237 pKa = 11.84 WFFVCSEE244 pKa = 4.17 EE245 pKa = 4.34
Molecular weight: 28.05 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.947
IPC2_protein 4.19
IPC_protein 4.19
Toseland 3.986
ProMoST 4.317
Dawson 4.177
Bjellqvist 4.355
Wikipedia 4.101
Rodwell 4.024
Grimsley 3.897
Solomon 4.177
Lehninger 4.126
Nozaki 4.279
DTASelect 4.533
Thurlkill 4.024
EMBOSS 4.113
Sillero 4.317
Patrickios 1.074
IPC_peptide 4.164
IPC2_peptide 4.291
IPC2.peptide.svr19 4.189
Protein with the highest isoelectric point:
>tr|A0A0C1PMJ3|A0A0C1PMJ3_9LACO DUF3324 domain-containing protein OS=Fructilactobacillus fructivorans OX=1614 GN=LfDm3_0645 PE=4 SV=1
MM1 pKa = 7.63 NFLTKK6 pKa = 10.59 NSLPIGFRR14 pKa = 11.84 KK15 pKa = 9.53 IKK17 pKa = 10.17 NIPNIVKK24 pKa = 10.46 NNVRR28 pKa = 11.84 VLRR31 pKa = 11.84 TIRR34 pKa = 11.84 NGLKK38 pKa = 10.31
Molecular weight: 4.42 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.357
IPC2_protein 10.789
IPC_protein 12.325
Toseland 12.501
ProMoST 12.983
Dawson 12.501
Bjellqvist 12.486
Wikipedia 12.969
Rodwell 12.398
Grimsley 12.544
Solomon 12.983
Lehninger 12.896
Nozaki 12.501
DTASelect 12.486
Thurlkill 12.501
EMBOSS 12.998
Sillero 12.501
Patrickios 12.149
IPC_peptide 12.983
IPC2_peptide 11.974
IPC2.peptide.svr19 9.008
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1281
0
1281
397748
37
3377
310.5
34.73
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.813 ± 0.061
0.445 ± 0.016
6.592 ± 0.08
5.157 ± 0.084
4.291 ± 0.068
6.696 ± 0.064
2.149 ± 0.029
7.623 ± 0.076
7.592 ± 0.082
8.867 ± 0.079
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.867 ± 0.032
5.788 ± 0.061
3.676 ± 0.045
4.107 ± 0.052
3.681 ± 0.061
6.515 ± 0.278
5.491 ± 0.071
7.204 ± 0.056
0.896 ± 0.028
3.551 ± 0.047
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here