Thiothrix caldifontis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Thiotrichales; Thiotrichaceae; Thiothrix

Average proteome isoelectric point is 6.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3757 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H4GV43|A0A1H4GV43_9GAMM Arsenite transporter ACR3 family OS=Thiothrix caldifontis OX=525918 GN=SAMN05660964_03740 PE=3 SV=1
MM1 pKa = 7.6KK2 pKa = 10.04IIKK5 pKa = 10.21LSLCISMVGAFATLPPAYY23 pKa = 10.06ANEE26 pKa = 4.16VVTNCTQVTDD36 pKa = 3.84TGGSDD41 pKa = 3.61SNTNNNEE48 pKa = 3.28SCAAVTIPFDD58 pKa = 4.17FGDD61 pKa = 3.99APEE64 pKa = 4.42TFPVLLANGGARR76 pKa = 11.84HH77 pKa = 5.1QLGTNVYY84 pKa = 9.95LGKK87 pKa = 10.72CVDD90 pKa = 3.71GDD92 pKa = 3.77SGEE95 pKa = 4.37LQGEE99 pKa = 4.42ASADD103 pKa = 3.74DD104 pKa = 4.25ADD106 pKa = 4.24EE107 pKa = 4.35SQPVYY112 pKa = 10.57GVCEE116 pKa = 3.58TDD118 pKa = 4.37AFGKK122 pKa = 10.23QDD124 pKa = 4.3DD125 pKa = 4.17EE126 pKa = 6.06DD127 pKa = 4.22GVAIAEE133 pKa = 4.23LRR135 pKa = 11.84VGDD138 pKa = 4.06TEE140 pKa = 4.35STVQVTTSADD150 pKa = 3.45CKK152 pKa = 10.56LNAWVDD158 pKa = 3.55WNQDD162 pKa = 3.51GSWGGAGEE170 pKa = 4.79QVFLDD175 pKa = 3.7QQLTAGLNNLTMDD188 pKa = 4.1VPAFAAEE195 pKa = 4.14GTAYY199 pKa = 9.71TRR201 pKa = 11.84FRR203 pKa = 11.84CSTAGGDD210 pKa = 4.19GIGGEE215 pKa = 4.37AIDD218 pKa = 6.03GEE220 pKa = 4.48VEE222 pKa = 4.28DD223 pKa = 4.53YY224 pKa = 11.24KK225 pKa = 11.08LTILPAIPKK234 pKa = 9.46VPVSVGDD241 pKa = 4.81KK242 pKa = 10.23IWIDD246 pKa = 3.5ANQNGQQDD254 pKa = 3.52ADD256 pKa = 3.82EE257 pKa = 4.8TGLAGATVTLLDD269 pKa = 4.21KK270 pKa = 11.15DD271 pKa = 4.14GNPVNDD277 pKa = 4.43LDD279 pKa = 4.45GKK281 pKa = 9.64PVAAVTTDD289 pKa = 2.7GGGAYY294 pKa = 10.42VFGNLAEE301 pKa = 4.31GQYY304 pKa = 10.41LIRR307 pKa = 11.84VEE309 pKa = 4.31TPEE312 pKa = 5.28GYY314 pKa = 10.75LPTQGGTDD322 pKa = 3.22VDD324 pKa = 4.44DD325 pKa = 5.16DD326 pKa = 4.88VSNTDD331 pKa = 3.47SNCQVVDD338 pKa = 4.11GKK340 pKa = 8.4TQTSDD345 pKa = 3.8FSLTTDD351 pKa = 4.1NMTVDD356 pKa = 4.11CGFYY360 pKa = 10.05MPTQPVHH367 pKa = 6.82SIGNMVWVDD376 pKa = 3.93DD377 pKa = 4.34GAGDD381 pKa = 4.39KK382 pKa = 10.61TNADD386 pKa = 3.04NGQFDD391 pKa = 3.93EE392 pKa = 6.55GEE394 pKa = 4.06ALLNGIKK401 pKa = 10.53LEE403 pKa = 4.38LRR405 pKa = 11.84DD406 pKa = 3.48TTGAVIEE413 pKa = 4.34STMTSGGYY421 pKa = 9.61YY422 pKa = 10.4LFSGLNAGEE431 pKa = 4.2YY432 pKa = 8.28QVCVAATNFKK442 pKa = 11.0GMGKK446 pKa = 9.82LVGYY450 pKa = 6.79TAGVNGKK457 pKa = 8.81EE458 pKa = 3.8ADD460 pKa = 3.64ANTGGDD466 pKa = 3.67SNDD469 pKa = 3.52NGGEE473 pKa = 3.99LSS475 pKa = 3.48

Molecular weight: 49.37 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H4G3C2|A0A1H4G3C2_9GAMM Sulfide dehydrogenase (Flavocytochrome c) flavoprotein subunit OS=Thiothrix caldifontis OX=525918 GN=SAMN05660964_03262 PE=4 SV=1
MM1 pKa = 7.62KK2 pKa = 10.25LYY4 pKa = 8.95KK5 pKa = 9.87TAFNDD10 pKa = 3.29ADD12 pKa = 3.73LSRR15 pKa = 11.84IVEE18 pKa = 4.35MAWEE22 pKa = 4.24DD23 pKa = 3.55RR24 pKa = 11.84TPFEE28 pKa = 5.94AINTLYY34 pKa = 10.92GLNEE38 pKa = 3.99TAVIDD43 pKa = 4.8LMRR46 pKa = 11.84QQMQPSSFRR55 pKa = 11.84MWRR58 pKa = 11.84KK59 pKa = 9.74RR60 pKa = 11.84MAGRR64 pKa = 11.84TTKK67 pKa = 10.53HH68 pKa = 5.0LGKK71 pKa = 10.38RR72 pKa = 11.84GFLVGRR78 pKa = 11.84FVCPSQRR85 pKa = 11.84TRR87 pKa = 3.22

Molecular weight: 10.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3757

0

3757

1212283

17

4234

322.7

35.68

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.925 ± 0.047

0.987 ± 0.013

5.437 ± 0.042

5.752 ± 0.033

3.771 ± 0.028

7.244 ± 0.044

2.445 ± 0.02

5.425 ± 0.033

4.328 ± 0.037

10.899 ± 0.068

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.545 ± 0.024

3.748 ± 0.039

4.652 ± 0.025

4.755 ± 0.036

5.402 ± 0.035

5.599 ± 0.031

5.808 ± 0.04

6.929 ± 0.034

1.428 ± 0.017

2.92 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski