Bacillus vietnamensis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Rossellomorea

Average proteome isoelectric point is 6.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4104 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0P6WFC2|A0A0P6WFC2_9BACI Holin OS=Bacillus vietnamensis OX=218284 GN=AM506_12920 PE=3 SV=1
MM1 pKa = 7.83AFPSNAQYY9 pKa = 10.86TPVLVGGSPLFDD21 pKa = 3.39VLGDD25 pKa = 3.8EE26 pKa = 5.19SPVSTDD32 pKa = 2.46IVGNSTFPAGFFAYY46 pKa = 10.1DD47 pKa = 3.26GTNVYY52 pKa = 9.92FRR54 pKa = 11.84LRR56 pKa = 11.84LNGDD60 pKa = 3.18PRR62 pKa = 11.84NNQLTGFRR70 pKa = 11.84NFAWGVLINTTGVAGTYY87 pKa = 10.17DD88 pKa = 3.36WLFNIDD94 pKa = 3.6GLNNRR99 pKa = 11.84VSLIQNTVKK108 pKa = 10.63LVNSWNDD115 pKa = 3.28PAEE118 pKa = 4.43GTGGGNPNFAQPITNFDD135 pKa = 3.84YY136 pKa = 11.39ARR138 pKa = 11.84VTPADD143 pKa = 3.55SSIGGDD149 pKa = 3.04QDD151 pKa = 5.45FFLDD155 pKa = 3.5WFLPASTVFSFLGINASSSIRR176 pKa = 11.84AVYY179 pKa = 10.09FSSANANNYY188 pKa = 9.83NKK190 pKa = 10.52DD191 pKa = 3.33SLRR194 pKa = 11.84TSEE197 pKa = 4.49GFSFANAFTDD207 pKa = 5.38PITPGQADD215 pKa = 3.0IRR217 pKa = 11.84ARR219 pKa = 11.84LATNKK224 pKa = 9.09VRR226 pKa = 11.84NSGPQTVVLGQQATWTGTVSITNTGLSQATSVFLDD261 pKa = 4.82DD262 pKa = 5.96IIGPDD267 pKa = 3.44QVNSFLVNSTSQGLTTYY284 pKa = 11.1NPTTKK289 pKa = 10.8LLTWNVGNLNPGATATLTFTLNGAFTTSGSRR320 pKa = 11.84NLDD323 pKa = 3.01RR324 pKa = 11.84VQATGFDD331 pKa = 3.88SSSGNAIQSNTSVVTINVQQTATINGTITDD361 pKa = 3.68QSTGLVLPNTTVSLLQGISIIATTQSNASGFYY393 pKa = 9.45TFTNVVPGNYY403 pKa = 8.15TVQAARR409 pKa = 11.84TNYY412 pKa = 8.13VTGTANVSAVGGTSTTADD430 pKa = 3.12IALVPQPSTISGNVSNGGPINNATVQLLNNSGTVVATTTTNVAGNYY476 pKa = 9.74SFVNVTPGLYY486 pKa = 10.19NVTVLASGFQSHH498 pKa = 6.09TKK500 pKa = 10.51SVMTEE505 pKa = 3.71PNQAAVVNFILIANPGSISGTVRR528 pKa = 11.84DD529 pKa = 4.02STNNTVIALANVEE542 pKa = 4.59LLDD545 pKa = 4.43SNGIPIASTTANGSGQYY562 pKa = 10.48SFNNLAPGNYY572 pKa = 7.27QVRR575 pKa = 11.84SFAANYY581 pKa = 7.58STTTVSSTVTAGNITNTNIFLEE603 pKa = 4.49PNPGSIQGTVIDD615 pKa = 4.84SEE617 pKa = 4.68TMSAITGASVQAVNSQNVIVASTITNGSGQYY648 pKa = 10.23SLSSLLPGSYY658 pKa = 10.8SLIFTANGYY667 pKa = 7.68ATQTLGAVVTSNAVTIVDD685 pKa = 3.61AALSKK690 pKa = 10.88LAGALIGTVQDD701 pKa = 4.16PNAVAIPGATVTVFQNNIQVGSVITDD727 pKa = 3.18SNGNYY732 pKa = 8.3MVSGLPPGSYY742 pKa = 8.92TVVVSAPNYY751 pKa = 5.7TTEE754 pKa = 4.22SVAAMIEE761 pKa = 4.07NGQTTTVNVTLNEE774 pKa = 4.35DD775 pKa = 3.67PGTLTGFVRR784 pKa = 11.84DD785 pKa = 4.18TNNVPIPGGSVTVQISTGAGIIVATTVTAPDD816 pKa = 3.51GSYY819 pKa = 9.22TVPNLSPGNYY829 pKa = 7.72TVVAAASNFQAATQGVTISSNTTSIVNFNLASDD862 pKa = 4.28PGSISGIVTNAQTGTPIIGANVQVRR887 pKa = 11.84VVDD890 pKa = 3.47SSGAVIATVLTDD902 pKa = 3.32EE903 pKa = 4.47NGQYY907 pKa = 9.93VVNGLAPGIYY917 pKa = 8.62TVVVSAQDD925 pKa = 3.58FQTNAATIQVISNQTRR941 pKa = 11.84DD942 pKa = 3.18GSIALQPDD950 pKa = 3.98PGQITGTVVDD960 pKa = 4.55SVGSNPIPGASVSIVNSSGNLITTVLTDD988 pKa = 3.39TNGVFMVEE996 pKa = 4.84GLAPDD1001 pKa = 3.88NYY1003 pKa = 9.18TVNVFANNFQNGMVGALVSSGQTTPVSIALNPDD1036 pKa = 3.55PGTITGTVSPQVPNTIVQLRR1056 pKa = 11.84DD1057 pKa = 3.4VNNVLIDD1064 pKa = 3.94SVVANQDD1071 pKa = 3.16GTFSFNNLTPGTYY1084 pKa = 8.9TVLASAPNYY1093 pKa = 8.52STAQAGVSVLANQTSTVGLTLVPNPGSVSGIVTDD1127 pKa = 3.96NLGSPIVNAIVQIFDD1142 pKa = 3.5QNNILIGSGFTDD1154 pKa = 2.9SSGQYY1159 pKa = 9.78IVGNLPSGSFNVVVNSPGFGQVITGINLGVGEE1191 pKa = 5.46DD1192 pKa = 3.58LTGVNISLIPNPGIIDD1208 pKa = 3.74GQITNFATGDD1218 pKa = 3.86TIAGATVVIIDD1229 pKa = 5.74GISQIPIATTTTSAFGNYY1247 pKa = 8.6SVSGLSPGSYY1257 pKa = 9.13IVSASKK1263 pKa = 10.62MNFTTEE1269 pKa = 3.81QIGAIVISDD1278 pKa = 3.49SATRR1282 pKa = 11.84ADD1284 pKa = 4.02LALGEE1289 pKa = 4.29NPGTISGNVEE1299 pKa = 3.95DD1300 pKa = 4.83TNGNPITGNGIQISVFNEE1318 pKa = 3.46NNVLVVSFLANSDD1331 pKa = 3.15GTYY1334 pKa = 10.28SVPSLAPGTYY1344 pKa = 9.92FITASAPNYY1353 pKa = 10.06SSSTVSAIVEE1363 pKa = 4.51SNQTTTVTNVLAANPVTLTVQVVIGNTSTPIEE1395 pKa = 4.41GSAVTIRR1402 pKa = 11.84HH1403 pKa = 5.85SNNIVIATGITDD1415 pKa = 3.55VNGLVTFFSLPTGTLNITADD1435 pKa = 3.47ASSFGTDD1442 pKa = 2.89TKK1444 pKa = 11.29SVIGGPGDD1452 pKa = 3.55SLSAVLSLAPNPGHH1466 pKa = 6.23IQGLVSNLASGEE1478 pKa = 4.29AIPNAVIQLYY1488 pKa = 10.6DD1489 pKa = 3.28ITNVLVQTTLSNQNGAYY1506 pKa = 9.64AFSGVTPGVYY1516 pKa = 9.02TVIANAADD1524 pKa = 4.67FGPEE1528 pKa = 3.41TAGAIVTSNQTSFLSFALSPNPGGVQGYY1556 pKa = 8.35VRR1558 pKa = 11.84NSVTLDD1564 pKa = 4.04PIVGATVVVRR1574 pKa = 11.84EE1575 pKa = 4.29LSGTGPVIFTTITDD1589 pKa = 3.27SDD1591 pKa = 4.27GFFQTTTLSPRR1602 pKa = 11.84VYY1604 pKa = 10.3VLVGSSPDD1612 pKa = 3.34FGSNSVSAEE1621 pKa = 3.92VMSGGVTNVEE1631 pKa = 3.79ILLNPNPGALTGTVRR1646 pKa = 11.84DD1647 pKa = 3.87AQTLQTLTDD1656 pKa = 3.46TLVRR1660 pKa = 11.84VINNQGTIVATVQTSIDD1677 pKa = 3.28GTYY1680 pKa = 9.91FIPGLTEE1687 pKa = 3.91GSYY1690 pKa = 9.32TVSAINTGYY1699 pKa = 10.59QSLLQQVNILPNSTIVLDD1717 pKa = 4.7FNLLANPATLSGTVVDD1733 pKa = 6.27AITGSPLTGVIIEE1746 pKa = 4.62VYY1748 pKa = 10.81VSGADD1753 pKa = 3.07ILVRR1757 pKa = 11.84RR1758 pKa = 11.84VLTDD1762 pKa = 3.03EE1763 pKa = 4.24NGNYY1767 pKa = 10.23LIEE1770 pKa = 4.6GLPQGTFDD1778 pKa = 5.74VKK1780 pKa = 11.12AQLQDD1785 pKa = 3.4YY1786 pKa = 8.98AISVNTVFLSPGEE1799 pKa = 4.14SEE1801 pKa = 4.55EE1802 pKa = 4.66LNIALVPFPATVQGTIRR1819 pKa = 11.84DD1820 pKa = 4.0AVTQDD1825 pKa = 4.3PISGALIKK1833 pKa = 10.62VVIPNTDD1840 pKa = 2.66IVVGSIITSSDD1851 pKa = 2.61GTYY1854 pKa = 10.75LIGNLPSGSYY1864 pKa = 10.39NVVISAEE1871 pKa = 4.53GYY1873 pKa = 7.47ATEE1876 pKa = 4.51VIPVILAPNGTEE1888 pKa = 4.16TVNADD1893 pKa = 4.45LDD1895 pKa = 4.26PNPAGITGFVLNAQTTVGIQGALVRR1920 pKa = 11.84VFNSDD1925 pKa = 2.92GVFIISTLTDD1935 pKa = 3.47DD1936 pKa = 3.74NGFYY1940 pKa = 10.29TISGLAQGQYY1950 pKa = 11.19AVIASADD1957 pKa = 3.54GFGDD1961 pKa = 3.59QIAIVTLSPGEE1972 pKa = 4.21TEE1974 pKa = 4.29SLNFSLSNQTATLRR1988 pKa = 11.84GTVRR1992 pKa = 11.84DD1993 pKa = 3.69AVSNQPIQSALVQVFRR2009 pKa = 11.84IGTSIPVASVLTDD2022 pKa = 3.51GSGKK2026 pKa = 10.48YY2027 pKa = 9.41EE2028 pKa = 4.04FTGLDD2033 pKa = 3.22PRR2035 pKa = 11.84EE2036 pKa = 3.95YY2037 pKa = 10.14RR2038 pKa = 11.84VVFSADD2044 pKa = 3.28GYY2046 pKa = 7.57TSEE2049 pKa = 4.15VFRR2052 pKa = 11.84VFLTNGEE2059 pKa = 4.32VQTLNAEE2066 pKa = 4.6LGRR2069 pKa = 11.84RR2070 pKa = 11.84PATIRR2075 pKa = 11.84GRR2077 pKa = 11.84VTDD2080 pKa = 4.33ANTGEE2085 pKa = 4.92PIQSAGVITVISGSGIIVASTLTDD2109 pKa = 3.23QEE2111 pKa = 4.25GNYY2114 pKa = 9.98ILTGLSAGDD2123 pKa = 3.54YY2124 pKa = 10.36NVIFSAVGYY2133 pKa = 9.69VSQTVMIRR2141 pKa = 11.84LSTGEE2146 pKa = 3.77MAIINAALEE2155 pKa = 4.52SNPATLTGFVRR2166 pKa = 11.84DD2167 pKa = 4.06AATLSPIEE2175 pKa = 3.97NALIQVFKK2183 pKa = 10.79PDD2185 pKa = 3.22GTLIGITVSDD2195 pKa = 3.59MNGNYY2200 pKa = 8.88TISGLPGGMLVIIATATGYY2219 pKa = 10.23QSQLQTVTLTPGTTSALNFLLADD2242 pKa = 3.75NPGSVSGTVTDD2253 pKa = 3.65IQTGEE2258 pKa = 4.75PISQVLVQIFPVGSLVPIRR2277 pKa = 11.84STLTDD2282 pKa = 3.45PGGFYY2287 pKa = 10.41ILTGLPPGTYY2297 pKa = 10.08VIRR2300 pKa = 11.84FTASGYY2306 pKa = 8.19PVKK2309 pKa = 10.48EE2310 pKa = 3.86VTIVLAEE2317 pKa = 4.15GEE2319 pKa = 4.13NRR2321 pKa = 11.84LLNVQLGEE2329 pKa = 4.45VIPPPPSNLRR2339 pKa = 11.84PEE2341 pKa = 4.83CISVEE2346 pKa = 4.03KK2347 pKa = 10.69LYY2349 pKa = 11.12DD2350 pKa = 3.44WVIATHH2356 pKa = 6.73NSTQAFGLSPDD2367 pKa = 3.36CRR2369 pKa = 11.84KK2370 pKa = 9.9LVNGLLEE2377 pKa = 4.46RR2378 pKa = 11.84GEE2380 pKa = 4.93GIDD2383 pKa = 4.08IQCQLDD2389 pKa = 3.69PSLSPGCSLISFEE2402 pKa = 4.81RR2403 pKa = 11.84GTPGTVEE2410 pKa = 3.32IRR2412 pKa = 11.84GEE2414 pKa = 4.18VKK2416 pKa = 10.75LLVTLSSRR2424 pKa = 11.84TDD2426 pKa = 3.29NAEE2429 pKa = 3.85TCTMEE2434 pKa = 3.97VPVYY2438 pKa = 9.88FDD2440 pKa = 4.75KK2441 pKa = 10.96IVAVCLPEE2449 pKa = 6.04GMDD2452 pKa = 3.76AGNVNCSIVDD2462 pKa = 4.06FKK2464 pKa = 11.34CRR2466 pKa = 11.84EE2467 pKa = 3.85KK2468 pKa = 11.18GVILTRR2474 pKa = 11.84DD2475 pKa = 3.17QAKK2478 pKa = 10.15IPFIACLEE2486 pKa = 4.06IEE2488 pKa = 4.17ILQPVTLEE2496 pKa = 3.98VLGEE2500 pKa = 4.01FCRR2503 pKa = 11.84PRR2505 pKa = 11.84TVVEE2509 pKa = 4.07RR2510 pKa = 11.84VKK2512 pKa = 10.87EE2513 pKa = 4.26DD2514 pKa = 3.41EE2515 pKa = 4.41DD2516 pKa = 4.0LCC2518 pKa = 6.47

Molecular weight: 260.34 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0P6WC62|A0A0P6WC62_9BACI Uncharacterized protein OS=Bacillus vietnamensis OX=218284 GN=AM506_16775 PE=4 SV=1
MM1 pKa = 7.44KK2 pKa = 9.6RR3 pKa = 11.84TFQPNNRR10 pKa = 11.84KK11 pKa = 9.34RR12 pKa = 11.84KK13 pKa = 8.16KK14 pKa = 9.1NHH16 pKa = 4.74GFRR19 pKa = 11.84ARR21 pKa = 11.84MSSKK25 pKa = 10.35NGRR28 pKa = 11.84NVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84SKK37 pKa = 10.59GRR39 pKa = 11.84KK40 pKa = 8.44VLSAA44 pKa = 4.05

Molecular weight: 5.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4104

0

4104

1185518

26

2518

288.9

32.43

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.579 ± 0.041

0.691 ± 0.012

5.306 ± 0.035

7.753 ± 0.05

4.57 ± 0.033

7.205 ± 0.041

2.224 ± 0.022

7.517 ± 0.042

6.784 ± 0.039

9.837 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.884 ± 0.02

4.024 ± 0.03

3.597 ± 0.021

3.505 ± 0.024

4.135 ± 0.031

6.33 ± 0.028

5.376 ± 0.037

7.15 ± 0.037

1.062 ± 0.015

3.472 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski