Pirellula sp. SH-Sr6A

Taxonomy: cellular organisms; Bacteria; PVC group; Planctomycetes; Planctomycetia; Pirellulales; Pirellulaceae; Pirellula; unclassified Pirellula

Average proteome isoelectric point is 6.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5207 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A142Y0E4|A0A142Y0E4_9BACT DNA topoisomerase (ATP-hydrolyzing) OS=Pirellula sp. SH-Sr6A OX=1632865 GN=gyrB_2 PE=4 SV=1
MM1 pKa = 7.48SIVSFSRR8 pKa = 11.84QALFSVLFGLLPGLCAIAQPPGPPSEE34 pKa = 4.21TLDD37 pKa = 4.46LVLQVSNGVPQSQTNASVGWQGFGLSVMDD66 pKa = 5.44ADD68 pKa = 5.19GEE70 pKa = 4.25NDD72 pKa = 3.53ADD74 pKa = 4.43PYY76 pKa = 11.23LSYY79 pKa = 11.48ASGSYY84 pKa = 9.92SVYY87 pKa = 10.44EE88 pKa = 4.1PANGAMGQATMQAGARR104 pKa = 11.84NIYY107 pKa = 9.25PAASTNLANLTADD120 pKa = 3.73GLFNLDD126 pKa = 3.52SDD128 pKa = 4.4PGVDD132 pKa = 3.52QIAWTGAWAQATYY145 pKa = 10.27EE146 pKa = 4.07VDD148 pKa = 3.22GTPQEE153 pKa = 5.11DD154 pKa = 4.15EE155 pKa = 4.46FLLATSLIVLEE166 pKa = 4.49INYY169 pKa = 10.04AGNNGFVDD177 pKa = 3.7MRR179 pKa = 11.84SDD181 pKa = 4.31QMSCSAGVTNLAALQIVFDD200 pKa = 3.85QDD202 pKa = 3.38LFEE205 pKa = 5.06PRR207 pKa = 11.84LIGWYY212 pKa = 9.55SKK214 pKa = 11.39YY215 pKa = 10.83EE216 pKa = 3.96NGEE219 pKa = 3.89NTIVNVNEE227 pKa = 4.03LATGITVVASRR238 pKa = 11.84RR239 pKa = 11.84VNRR242 pKa = 11.84NQVFQIGCDD251 pKa = 3.39ATSGSDD257 pKa = 3.46PVALGDD263 pKa = 4.0GATQSGTSIASATLAIDD280 pKa = 3.5AWYY283 pKa = 10.4EE284 pKa = 3.82EE285 pKa = 4.49

Molecular weight: 30.05 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A142Y6Q7|A0A142Y6Q7_9BACT 30S ribosomal protein S5 OS=Pirellula sp. SH-Sr6A OX=1632865 GN=rpsE PE=3 SV=1
MM1 pKa = 7.2VKK3 pKa = 10.04PHH5 pKa = 6.99RR6 pKa = 11.84KK7 pKa = 9.03LKK9 pKa = 10.01KK10 pKa = 9.34ANHH13 pKa = 5.42GQRR16 pKa = 11.84AANSKK21 pKa = 9.65ARR23 pKa = 11.84KK24 pKa = 8.15AKK26 pKa = 9.29RR27 pKa = 11.84RR28 pKa = 11.84KK29 pKa = 9.63VRR31 pKa = 11.84TT32 pKa = 3.48

Molecular weight: 3.75 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5207

0

5207

1912954

29

6457

367.4

40.64

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.071 ± 0.036

1.193 ± 0.016

5.564 ± 0.025

6.318 ± 0.034

3.756 ± 0.02

7.449 ± 0.041

2.125 ± 0.018

5.393 ± 0.022

4.146 ± 0.032

9.862 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.251 ± 0.016

3.313 ± 0.027

5.268 ± 0.027

4.06 ± 0.022

6.671 ± 0.037

7.287 ± 0.029

5.35 ± 0.044

6.825 ± 0.031

1.692 ± 0.016

2.407 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski